28-10-2015 дата публикации
Номер: KR101563826B1
The present invention relates to a method for clearing a tissue, which comprises the steps of: perfusing a tissue with hydrogel to make incubation; and performing electrophoresis on the tissue perfused with the hydrogel to replace a lipid bilayer with hydrogel. The method for clearing a tissue according to the present invention could be applied to various parts of the whole brain, and even Purkinje cell layer of cerebellum could be observed through the cleared brain tissues. In addition, the method could be applied to various tissues such as a pancreas, a liver, a lung, a small intestines, a colon, and a kidney, wherein a complete structural relation between cells and blood vessels at the end part of a pancreas was observed by a monophoton microscope; and accordingly, an objective image of complex islet-blood relation was obtained. Moreover, formation of bubbles, discoloration and black deposit shown in a conventional method for clearing a tissue were not shown, and rather transparency ...
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