11-03-2021 дата публикации
Номер: US20210072220A1
Принадлежит:
A method to determine the throughput speed v of a pore, comprising the steps of feeding, by means of a driving force F, a filiform calibration element through the pore, the calibration element having a plurality of markers spaced apart by known distances and configured to produce an interaction event that transmits a signal away from the pore upon interaction with the pore, detecting a plurality of interaction events, and determining a time interval Δt between successive interaction events, and/or a frequency ω of interaction events. 1. A method to determine the throughput speed v of a pore , comprising the steps of:feeding, by means of a driving force F, a filiform calibration element through the pore, the calibration element having a plurality of markers spaced apart by known distances and configured to produce an interaction event that transmits a signal away from the pore upon interaction with the pore;detecting a plurality of interaction events; anddetermining a time interval Δt between successive interaction events, and/or a frequency ω of interaction events.2. The method of claim 1 , wherein a condition for production of the event by a marker is fulfilled at most in an interval along the path that the marker follows when the filiform calibration element is fed through the pore claim 1 , wherein the length of this interval is at most 20% of the length of the pore.3. The method of claim 1 , further comprising:determining, from the known distances and from the time interval Δt and/or the frequency ω, the speed with which the filiform calibration element passes through the pore as the throughput speed v.4. The method of claim 3 , further comprising: determining a throughput resistance R of the pore as a ratio of the speed v per unit of the driving force F.5. The method of claim 1 , further comprising:detecting at least one increase in the mechanical resistance that the filiform calibration element experiences when moving through the pore as an interaction event.6 ...
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