Method for chemical amplification based on fluid partitioning in an immiscible liquid

A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.

Micro-fluidic partitioning between polymeric sheets for chemical amplification and processing

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations.

MICRO-FLUIDIC PARTITIONING BETWEEN POLYMERIC SHEETS FOR CHEMICAL AMPLIFICATION AND PROCESSING

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations. 1. An apparatus for fluid partitioning for chemical amplification or other chemical processing or separations , comprising:two polymeric sheets,fluid to be partition between said two polymeric sheets, anda seal sealing the sheets together.2. The apparatus for fluid partitioning of wherein said seal forms an array of independent fluid volumes that do not have fluid communication.3. The apparatus for fluid partitioning of wherein said seal forms an array of independent fluid volumes between one milli-liter and one pico-liter that do not have fluid communication.4. The apparatus for fluid partitioning of wherein said seal sealing the sheets together is a thermal bond.5. The apparatus for fluid partitioning of wherein said seal sealing the sheets together is a thermal bond formed by thermal bonding to form an array of independent fluid volumes that do not have fluid communication.6. An apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of a sample claim 1 , comprising:a first dispenser of a first polymeric sheet;a second dispenser of a second polymeric sheet wherein said first dispenser and said second ...

Method of Developing a Lithographic Printing Plate Including Post Treatment

A method for producing a lithographic plate from a negative working, radiation imageable plate having an oleophilic resin coating that reacts to radiation by cross linking and is non-ionically adhered to a hydrophilic substrate. The steps include imagewise radiation exposing the coating to produce an imaged plate having partially reacted image areas at an initial double bond conversion rate including unreacted coating material, and completely unreacted nonimage areas; developing the plate by removing only the unreacted, nonimage areas from the substrate while retaining unreacted material in the image areas; and subjecting the upper surface of the plate to blanket post treatment energy, which further reacts the retained unreacted material in the image areas to increase the initial double bond conversion rate, producing a lithographic printing plate with enhanced durability. 1. A method for pre-press production of a lithographic printing plate from a negative working , radiation imageable plate having an oleophilic resin coating material containing active ingredients that participate in cross-linking reactions to radiation and is non-ionically adhered to a hydrophilic substrate , comprising the steps of:(a) imagewise radiation exposing the coating to produce an imaged plate having image areas with partially reacted resin material at an initial double bond conversion per cent and nonimage areas of resin material that are completely unreacted;(b) without preheating, developing the plate with brushes in an aqueous solution to remove only the unreacted, nonimage areas from the substrate while retaining unreacted material in the image areas; and(c) blanket exposing the developed plate to an external source of energy which further reacts the retained unreacted material in the image areas, thereby increasing the initial double bond conversion by at least about 5 per cent.2. The method of claim 1 , wherein the external source of energy in step (c) is at least one of IR and UV ...

METHOD OF CONCURRENTLY FILTERING PARTICLES AND COLLECTING GASES

A system for concurrently filtering particles and collecting gases. Materials are be added (e.g., via coating the ceramic substrate, use of loose powder(s), or other means) to a HEPA filter (ceramic, metal, or otherwise) to collect gases (e.g., radioactive gases such as iodine). The gases could be radioactive, hazardous, or valuable gases. 1. A filter apparatus for concurrently removing particles from a fluid and removing gases from the fluid , comprising:a filter unit body housing;at least one filter element positioned in said filter unit body housing,a filter material associated with said at least one filter element that removes the particles from the fluid, anda material associated with said at least one filter element that removes the gases from the fluid.2. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said at least one filter element positioned in said filter unit body housing is a filter element that includes nanofibers.3. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said at least one filter element positioned in said filter unit body housing is a flat filter element.4. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said at least one filter element positioned in said filter unit body housing is a tubular filter element positioned in said filter unit body housing with a filter material associated with said tubular filter element that removes the particles from the fluid.5. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said material that removes the gases from the fluid includes natural enzymes.6. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said material that removes the gases from the fluid includes zeolites.7. The filter apparatus for concurrently removing particles and gases from a fluid of wherein said material that removes the gases ...

MICRO-FLUIDIC PARTITIONING BETWEEN POLYMERIC SHEETS FOR CHEMICAL AMPLIFICATION AND PROCESSING

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations. 1. An apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of a sample , comprising:a first dispenser of a first polymeric sheet;a second dispenser of a second polymeric sheet wherein said first dispenser and said second dispenser are positioned so that said first polymeric sheet and said second polymeric sheet become parallel;a dispenser of the sample positioned to dispense the sample between said first polymeric sheet and said second polymeric sheet; anda seal unit that seals said first polymeric sheet and said second polymeric sheet together thereby sealing the sample between said first polymeric sheet and said second polymeric sheet and partitioning the sample for chemical amplification or other chemical processing or separations, wherein seal unit that seals said first polymeric sheet and said second polymeric sheet together comprises a roller with grid projections that forms a grid array in said first polymeric sheet and said second polymeric sheet.2. An apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of a sample , comprising:a first ...

Chemical amplification based on fluid partitioning

A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

An aqueous ink jet receiving medium which yields a water resistant ink jet print and a process for providing a water resistant ink jet print are disclosed. The water resistant ink jet receiving medium comprises an ink receptive layer of a crosslinked vinyl amide acrylic acid or methacrylic acid or ester thereof random copolymer and a cationic resin. The random copolymer employed is preferably a vinyl pyrrolidone/acrylic acid or acrylamide/acrylic acid copolymer.

Clamp assembly for clamping a seat in position

A clamp assembly for clamping a seat (32) in position comprising a post (18) to one end of which there is secured a clamp tube (20) whose axis is substantially perpendicular to that of the post (18); a clamp device (22) which is mounted in the clamp tube (20) and which has a plurality of separate parts (24,26,28) between which seat support means (30) may be disposed; and clamp means (34) for clamping the separate parts (24,26,28) together so as to clamp the seat support means (30) therebetween and simultaneously to clamp the clamp device (22) as a whole to the clamp tube (20).

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

Ink jet receptor element having a protective layer

An ink jet image receptor element having a protective coating is disclosed. The protective coating is a single protective layer that contains a fluoropolymer and an acrylic polymer. Because the protective coating is resistant to surface pitting, dirt, stains, and general degradation, images formed from the element can be used effectively on billboards, banners, posters, and other outdoor signs.

Chemical amplification based on fluid partitioning

A system for nucleic acid amplification of a sample comprises partitioning the sample into partitioned sections and performing PCR on the partitioned sections of the sample. Another embodiment of the invention provides a system for nucleic acid amplification and detection of a sample comprising partitioning the sample into partitioned sections, performing PCR on the partitioned sections of the sample, and detecting and analyzing the partitioned sections of the sample.

Wässriges tintenaufnahme- tintenstrahlempfangsmedium, welches einen wasserbeständigen tintenstrahldruck ergibt

Wässriges tintenaufnahme- tintenstrahlempfangsmedium, welches einen wasserbeständigen tintenstrahldruck erzeugt

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

Provided is a receiving medium comprising a substrate and an ink receiving layer provided thereon, said ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinyl alcohol. The ink receiving layer preferably further comprises a solid particulate such as silica or calcium silicate. Also provided is a process for providing a water resistant ink jet print by attaching droplets of a recording liquid on a recording medium, said recording medium comprising an ink receiving layer provided on a substrate with the ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinylalcohol.

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

Provided is a receiving medium comprising a substrate and an ink receiving layer provided thereon, said ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinyl alcohol. The ink receiving layer preferably further comprises a solid particulate such as silica or calcium silicate. Also provided is a process for providing a water resistant ink jet print by attaching droplets of a recording liquid on a recording medium, said recording medium comprising an ink receiving layer provided on a substrate with the ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinylalcohol.

Aqueous ink receptive ink jet receiving medium yielding a water resistant ink jet print

Provided is a receiving medium comprising a substrate and an ink receiving layer provided thereon, said ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinyl alcohol. The ink receiving layer preferably further comprises a solid particulate such as silica or calcium silicate. Also provided is a process for providing a water resistant ink jet print by attaching droplets of a recording liquid on a recording medium, said recording medium comprising an ink receiving layer provided on a substrate with the ink receiving layer comprising a blend of an ethylene vinylacetate copolymer and a hydrolyzed polyvinylalcohol.