SELECTIVE DISTANCE OF IMMUNE COMPLEXES.

SELECTIVE REMOVAL OF IMMUNE COMPLEXES

SELECTIVE REMOVAL OF IMMUNE COMPLEXES

Disclosed is a method and a family of materials useful for removing immune complexes from blood preferentially to soluble antibodies. The material comprises analogs of proteins which bind to the Fc region of immunoglobulin. The analogs are produced by truncating or otherwise altering the amino acid sequence of the binding protein to reduce their affinity for Fc. An array of such analogs disposed about the surface of an insoluble matrix has the ability to form multiple points of attachment to the multiple Fc's in a complex so as to bind complex strongly, whereas only weak associations are developed between the Fc region of soluble IgG and individual analogs. The preferred analogs are truncated proteins homologous to a portion of the domains of Protein A or Protein G which bind with Fc. Complex may be removed from whole blood or serum using the material and conventional plasmapheresis techniques.

SELEKTIVE ENTFERNUNG VON IMMUNKOMPLEXEN.

SELEKTIVE ENTFERNUNG VON IMMUNKOMPLEXEN.

SAMPLE PREPARATION FOR DIFFICULT SAMPLE TYPES

Devices and methods are provided for collecting and handling difficult sample types. 1. A method of amplifying RNA from a direct blood sample comprising:(a) adding the direct blood sample to a sample buffer,(b) bead beating the sample buffer,(c) extracting the RNA from the sample buffer, and(d) amplifying the RNA.2. The method of claim 1 , wherein steps (b) through (d) are performed in a closed sample vessel.3. The method of claim 1 , wherein steps (a) through (d) are performed without centrifugation claim 1 , ethanol precipitation claim 1 , and DNA digestion.4. The method of claim 1 , wherein the RNA is host RNA.5. The method of claim 1 , wherein the direct blood sample is collected in a RNA stabilized solution.6. The method of claim 1 , wherein the direct blood sample is collected in a non-RNA stabilizing solution.7. The method of claim 1 , wherein the direct blood sample is separated prior to step (a) and a blood fraction is used.8. The method of claim 1 , further comprising filtering the sample between steps (a) and (b).9. The method of claim 1 , wherein the RNA is provided in high copy number.10. The method of claim 1 , wherein the RNA is from microorganisms.11. The method of claim 1 , further comprising washing the RNA following the extracting step claim 1 , wherein the washing step comprises no more than three washes.12. The method of claim 1 , wherein the sample buffer includes a guanidinium compound.13. The method of claim 1 , wherein step (g) includes a first-stage multiplex PCR and a plurality of individual second-stage PCR reactions. This application is a divisional application of U.S. patent application Ser. No. 15/340,612, filed Nov. 1, 2016, which claims the benefit, under 35 U.S.C. § 119(e), of U.S. Provisional Application Ser. No. 62/249,592, filed Nov. 2, 2015, the entire contents of each of which are incorporated by reference herein.Embodiments of the present disclosure relate generally to methods and devices for extracting nucleic acids from a ...

SAMPLE PREPARATION FOR DIFFICULT SAMPLE TYPES

Devices and methods are provided for collecting and handling difficult sample types. 1. A sample collection method comprising:collecting a sample comprising a biological or environmental substance using a swab,placing the swab with the collected sample in a sample buffer, andfiltering the sample.2. The sample collection method of further comprising contacting the sample in the sample buffer with a protease.3. The sample collection method of wherein the swab is a flocked swab.4. The sample collection method of wherein the collecting step comprises collecting the sample from a sample vessel without pipetting and the swab is configured to absorb and release an amount of the sample within a four-fold volume range.5. (canceled)6. The sample collection method of wherein the substance is a substance that has a viscosity of 3 cP or greater.7. (canceled)8. The sample collection method of wherein the sample buffer comprises a detergent in an amount of at least 10% by volume of the sample buffer.910.-. (canceled)11. The sample collection method of wherein the sample buffer comprises 15% Triton-X by volume of sample buffer.12. The sample collection method of wherein the filtering step comprises drawing the sample through a filter in the presence of a protease.1314.-. (canceled)15. The sample collection method of wherein the sample comprises a sample matrix and a pathogen claim 1 , wherein the swab preferentially retains the sample matrix while preferentially releasing the pathogen into the sample buffer.16. The sample collection method of wherein there is no pretreatment step of more than 5 minutes.17. (canceled)18. The sample collection method of wherein the sample is sputum and the sputum is not heat treated or treated with DTT prior to the placing step.1920.-. (canceled)21. A sample collection method comprising:collecting a sample comprising a biological or environmental substance, andplacing the sample in a sample buffer wherein the sample buffer comprises a detergent in an ...

CMOS INTEGRATED CIRCUIT AND MANUFACTURING METHOD

METHODS FOR SAMPLE USE

PREPARAÇÃO DE AMOSTRA PARA TIPOS DIFÍCEIS DE AMOSTRA. Trata-se de dispositivos e métodos para coletar e manusear tipos difíceis de amostra. Em um aspecto da presente descrição, um método de coleta de amostra é fornecido, sendo que o método compreende coletar uma amostra que compreende uma substância biológica ou ambiental através do uso de um swab, dispor o swab com a amostra coletada em um tampão de amostra e filtrar a amostra. Em várias modalidades ilustrativas, o tampão de amostra pode incluir uma protease ou um detergente e a amostra pode ser coletada e transferida para o tampão de amostra através do uso de um swab flocado. SAMPLE PREPARATION FOR DIFFICULT SAMPLE TYPES. These are devices and methods for collecting and handling difficult types of samples. In one aspect of the present description, a sample collection method is provided, the method comprising collecting a sample comprising a biological or environmental substance through the use of a swab, disposing the swab with the collected sample in a sample buffer and filter the sample. In various illustrative embodiments, the sample buffer may include a protease or a detergent and the sample may be collected and transferred to the sample buffer using a flocked swab.

Method of making taps and dies

Sample preparation for difficult sample types

Sample preparation for difficult sample types

Devices and methods are provided for collecting and handling difficult sample types. In one aspect of the present disclosure, a sample collection method is provided, the method comprising collecting a sample comprising a biological or environmental substance using a swab, placing the swab with the collected sample in a sample buffer, and filtering the sample. In various illustrative embodiments, the sample buffer may include a protease or a detergent and the sample may be collected and transferred to the sample buffer using a flocked swab.

Rapid cell lysis and nucleic acid recovery

Methods and systems for rapid preparation of a nucleic acid sample.

Method of amplifying nucleic acids

Rapid cell lysis and nucleic acid recovery

Methods and systems for rapid preparation of a nucleic acid sample.

Method of amplifying nucleic acids

Pump