Anesthesia device for animal experiment

The invention discloses an anesthesia device for an animal experiment. The device is characterized by comprising an anesthesia case, a closed vessel filled with a volatile anesthetic; a seal door which can be opened is formed on the front side of the anesthesia case; communicating pipes which are communicated with the inside and the outside of the anesthesia case are arranged on two sides of the anesthesia case respectively; the communicating pipe on one side is arranged on the middle lower part of the case wall, and the communicating pipe on the other side is arranged on the upper part of the case wall; a sealing cover is arranged on the top of the closed vessel; and two straight pipes communicating the inside and the outside of the volatile anesthetic vessel are embedded on the sealing cover, one straight pipe is connected with the communicating pipe on the middle lower part of the case wall on one side of the anesthesia case through an anesthetic recycle device, and the other straight ...

Processing technique of artificial crystal diamond semi-finished products

Device for quantitatively counting body fluid

The invention discloses a device for quantitatively counting body fluid, comprising a liquid receiving vessel, a liquid receiving tube and a hole enlargement part of the liquid receiving tube, wherein the liquid receiving vessel is axially connected with the hole enlargement part of the liquid receiving tube in a detachable manner; the inner cavity of the hole enlargement part of the liquid receiving tube is provided with a positive electrode and a negative electrode which are electrically connected to a binding post which is symmetrical to the outboard diameter direction of the upper part ofthe hole enlargement part of the liquid receiving tube. The invention is characterized in that the liquid receiving tube is an inverted round table structure; the pipe orifice at the lower part of the round table is communicated with the inner cavity of the hole enlargement part of the liquid receiving tube; the positive electrode and negative electrode comprise a first wire electrode and a second wire ...

Device for protein separation and purification teaching experiment

The invention discloses a device for a protein separation and purification teaching experiment, which comprises a perfusion container, wherein a stirrer is arranged in the perfusion container; and the bottom of the container is provided with a drainage tube. The device is characterized in that a rotating disk is arranged below the perfusion container, and a plurality of chromatographic column test tubes are arranged on the circumference of the rotating disc; when each chromatographic column test tube is static, an opening at the upper end is over against an outlet of the drainage tube; the side wall of the drainage tube is provided with a switch plug; the switch plug and the rotating disk realize linkage by a flow control and steering device, i.e. when the switch plug closes the drainage tube, the rotating disk rotates, the chromatographic column test tube which is over against the outlet of the drainage tube is separated, and the adjacent chromatographic column test tube moves to theoutlet ...

Device for quantitatively counting body fluid

The invention discloses a device for quantitatively counting body fluid, comprising a liquid receiving vessel, a liquid receiving tube and a hole enlargement part of the liquid receiving tube, wherein the liquid receiving vessel is axially connected with the hole enlargement part of the liquid receiving tube in a detachable manner; the inner cavity of the hole enlargement part of the liquid receiving tube is provided with a positive electrode and a negative electrode which are electrically connected to a binding post which is symmetrical to the outboard diameter direction of the upper part of the hole enlargement part of the liquid receiving tube. The invention is characterized in that the liquid receiving tube is an inverted round table structure; the pipe orifice at the lower part of the round table is communicated with the inner cavity of the hole enlargement part of the liquid receiving tube; the positive electrode and negative electrode comprise a first wire electrode and a second ...

Processing technique of artificial crystal diamond semi-finished products

Externally-applied ointment for treating burns and scalds and preparation method

The invention discloses an externally-applied ointment for treating burns and scalds and a preparation method. The externally-applied ointment comprises the following components by weight percent: 30-40% of cereus, 5-15% of artemisia rupestris L extractive and 50-60% of badger fat. The preparation method of the externally-applied ointment for treating the burns and the scalds comprises the following steps: firstly, peeling the cereus, crushing, drying in the shade for later use and then drying the artemisia rupestris L extractive at the temperature of 80 DEG C for later use; then, heating the badger fat to the temperature of 80 DEG C, pouring into a container, adding the artemisia rupestris L extractive which is for later use into the badger fat slowly, stirring along with adding, then adding cereus which is for later use, stirring into a paste shape and cooling so as to prepare the externally-applied ointment for treating the burns and the scalds after keeping the temperature and stirring ...

Externally-applied ointment for treating burns and scalds and preparation method

The invention discloses an externally-applied ointment for treating burns and scalds and a preparation method. The externally-applied ointment comprises the following components by weight percent: 30-40% of cereus, 5-15% of artemisia rupestris L extractive and 50-60% of badger fat. The preparation method of the externally-applied ointment for treating the burns and the scalds comprises the following steps: firstly, peeling the cereus, crushing, drying in the shade for later use and then drying the artemisia rupestris L extractive at the temperature of 80 DEG C for later use; then, heating thebadger fat to the temperature of 80 DEG C, pouring into a container, adding the artemisia rupestris L extractive which is for later use into the badger fat slowly, stirring along with adding, then adding cereus which is for later use, stirring into a paste shape and cooling so as to prepare the externally-applied ointment for treating the burns and the scalds after keeping the temperature and stirring ...

Device for preparing plating medium

The invention discloses a device for preparing plating medium, comprising a flask; wherein the flask is filled with culture solution in constant temperature state prepared in advance, and the flask is internally provided with a transfer pipe extending to the outside of the bottleneck. The device is characterized in that the bottleneck is provided with a sealing plug, the transfer pipe is communicated with a suction unit with a negative pressure port outside the bottleneck through the sealing plug, an outlet of the suction unit is led to a quantitative perfusion device which is arranged on a worktable in a directing way by a flexible pipe and is lower the flask, the constant temperature culture solution in the flask is added into the quantitative perfusion device through the flexible pipe under the siphon action of the suction unit and is shunted to the required culture dish by the quantitative perfusion device, so as to form culture media with different diameters and thicknesses.

Device for preparing plating medium

The invention discloses a device for preparing plating medium, comprising a flask; wherein the flask is filled with culture solution in constant temperature state prepared in advance, and the flask is internally provided with a transfer pipe extending to the outside of the bottleneck. The device is characterized in that the bottleneck is provided with a sealing plug, the transfer pipe is communicated with a suction unit with a negative pressure port outside the bottleneck through the sealing plug, an outlet of the suction unit is led to a quantitative perfusion device which is arranged on a worktable in a directing way by a flexible pipe and is lower the flask, the constant temperature culture solution in the flask is added into the quantitative perfusion device through the flexible pipe under the siphon action of the suction unit and is shunted to the required culture dish by the quantitative perfusion device, so as to form culture media with different diameters and thicknesses.

Tetracycline stringent controlled eucaryon expression vector

The invention provides a tetracycline strictly controlled eukaryotic expression vector. Following DNA elements including hCMV, rtTA2-M2, IRES, EGFP-Puro, element (Luc) of coding luciferase and Ptetbi-RFC are respectively introduced and are used for the expression and screening of exogenous interest genes in eukaryotic cells. The vector is strictly controlled by Tet or Dox after being transfected. And the expression level of the genes is quantitatively regulated through the amount of the added Tet or Dox. The expression level of the interest genes can be indirectly detected through the detection of the activity of Luc luciferase. The transfection efficiency of the vector can be completed through the detection of the proportion of green fluorescyte. The stably transfected cells are sorted by a flow cytometry. Thus, the expression of the interest genes is ensured to be simple and convenient. And in a stable cell line obtained through screening, the interest genes are integrated with a host ...