Recombinant fluorescent nano antibody and application thereof in preparation of rabies virus detection reagent

The invention relates to a recombinant fluorescent nano-antibody and application thereof in preparation of a rabies virus detection reagent, and belongs to the technical field of biomolecular diagnostics, a yeast two-hybrid system is utilized to screen out a nano-antibody with strong binding force with rabies virus G protein, and an enhanced green fluorescent protein (EGFP) gene and six histidine (6 * His) tags are connected to the C terminal of the nano-antibody. An antibody fusion protein gene is cloned to a prokaryotic expression vector pMal-p5x, and expression is carried out through a prokaryotic expression engineering bacterium Shuffle T7-B. A fluorescent protein labeled nano antibody obtained through prokaryotic expression and purification is applied to direct immunofluorescence detection (DFA) of rabies virus instead of a fluorescein labeled antibody.