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Применить Всего найдено 4. Отображено 4.
18-07-2013 дата публикации

MATERIALS AND METHOD FOR DETECTING CYTOMEGALOVIRUS (CMV)

Номер: US20130183657A1
Автор: Barry Catherine P., Ho Shiaolan Y.
Принадлежит: ABBOTT LABORATORIES

Methods and kits for amplifying cytomegalovirus (CMV) nucleic acid sequences in a sample comprising pairs of primers for amplification of UL34 and UL80.5 nucleic acid sequences or one or more reagents. 1. A method of amplifying cytomegalovirus (CMV) nucleic acid sequences in a sample , which method comprises:(a) forming a mixture comprising the sample, nucleic acid amplification reagents, a pair of primers for amplification of a UL34 nucleic acid sequence, and a pair of primers for amplification of a UL80.5 nucleic acid sequence, and(b) subjecting the mixture to conditions that promote amplification of the UL34 nucleic acid sequence and the UL80.5 nucleic acid sequence,whereupon CMV nucleic acid sequences in a sample are amplified.4. The method of claim 1 , wherein the mixture in (a) further comprises an internal control (IC) nucleic acid and a pair of primers for amplifying the IC nucleic acid and wherein the conditions in (b) also promote amplification of the IC nucleic acid claim 1 , whereupon the IC nucleic acid sequence is amplified.5. The method of claim 1 , which further comprises simultaneously or subsequently detecting the presence claim 1 , amount or concentration of CMV in the sample claim 1 , whereupon CMV is detected in the sample.6. The method of claim 5 , wherein the mixture in (a) further comprises a probe for detection of amplification of a UL34 nucleic acid sequence and a probe for detection of amplification of a UL80.5 nucleic acid sequence claim 5 , wherein the probe is optionally labeled with FAM and quenched with BHQ-1.9. The method of claim 4 , which further comprises simultaneously or subsequently detecting the presence claim 4 , amount or concentration of IC in the mixture claim 4 , whereupon IC is detected in the mixture.10. A set of primers comprising a pair of forward and reverse primers for amplification of a CMV UL34 nucleic acid sequence and a pair of forward and reverse primers for amplification of a CMV UL80.5 nucleic acid sequence. ...

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Номер записи: 1
06-02-2020 дата публикации

SOLVENT COMPOSITION, ADHESIVE COMPOSITION, AND METHOD OF BONDING SURFACES

Номер: US20200040232A1
Автор: Barry Catherine P., Hansen Christopher, MOROSE Gregory
Принадлежит:

In an embodiment a solvent composition can comprise, based on the total volume of the solvent composition, 10 to 95 volume percent of a first solvent, wherein the first solvent has Hansen solubility parameters of: 15 MPa≤δ≤17 MPa, 4≤δ≤10.5 MPa, and 7≤δ≤10 MPa; 5 to 95 volume percent of a second solvent, wherein the second solvent has Hansen solubility parameters of: 16 MPa≤δ≤17.5 MPa, 0≤δ≤3 MPa, and 0≤δ≤3 MPa; and 0 to 85 volume percent parachlorobenzotrifluoride; wherein the amounts of the first solvent, the second solvent, and parachlorobenzotrifluoride sum to at least 85 volume percent. The first solvent can comprise methyl acetate, acetone, dimethyl carbonate, ethyl acetate, n-butylamine, propyl acetate, tetrahydrofuran, or a combination thereof. The second solvent can comprise cyclohexene, cyclohexane, cyclopentane, methylcyclohexane, or a combination thereof. The solvent compositions are particularly useful in an adhesive composition. An adhesive composition includes the solvent composition, a rubber, and a tackifying resin. A method of bonding two surfaces is also described. 1. A solvent composition comprising , based on the total volume of the solvent composition , {'sup': 0.5', '0.5', '0.5', '0.5, 'sub': D', 'P', 'H, '15 MPa≤δ≤17 MPa, 4≤δ≤10.5 MPa, and 7≤δ≤10 MPa;'}, '10 to 95 volume percent of a first solvent, wherein the first solvent has Hansen solubility parameters of {'sup': 0.5', '0.5', '0.5', '0.5, 'sub': D', 'P', 'H, '16 MPa≤δ≤17.5 MPa, 0≤δ≤3 MPa, and 0≤δ≤3 MPa; and'}, '5 to 95 volume percent of a second solvent, wherein the second solvent has Hansen solubility parameters of0 to 85 volume percent parachlorobenzotrifluoride;wherein the amounts of the first solvent, the second solvent, and parachlorobenzotrifluoride sum to at least 85 volume percent.2. A solvent composition comprising , based on the total volume of the solvent composition ,10 to 95 volume percent of a first solvent, wherein the first solvent consists essentially of methyl acetate, ...

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Номер записи: 2
19-07-2012 дата публикации

Materials and method for detecting cytomegalovirus (cmv)

Номер: CA2820067A1
Автор: Catherine P. Barry, Shiaolan Y. Ho
Принадлежит: Abbott Molecular Inc

A method of amplifying cytomegalovirus (CMV) nucleic acid sequences in a sample comprising (a) forming a mixture comprising the sample, amplification reagents, and pairs of primers for amplification of UL34 and UL80.5 nucleic acid sequences, and (b) subjecting the mixture to conditions that promote amplification, wherein the method can further comprise (c) detecting the presence, amount or concentration of CMV in the sample, in which case the mixture in (a) can further comprise probes for detection of amplification of UL34 and UL80.5 nucleic acid sequences; a set of primers comprising pairs of forward and reverse primers for amplification of UL34 and UL80.5 nucleic acid sequences; a set of probes comprising probes for detection of amplification of UL34 and UL80.5 nucleic acid sequences; and a kit comprising (i) sets of primers for amplification of UL34 and UL80.5 nucleic acid sequences and (ii) instructions for using the sets of primers and/or one or more reagents for using the sets of primers, wherein the kit can further comprise (i) probes for detection of amplification of UL34 and UL80.5 nucleic acid sequences and (ii) instructions for using the probes and/or one or more reagents for using the probes.

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Номер записи: 3
04-10-2018 дата публикации

Solvent composition, adhesive composition, and method of bonding surfaces

Номер: WO2018183990A1
Автор: Catherine P. Barry, Christopher Hansen, Gregory MOROSE
Принадлежит: THE UNIVERSITY OF MASSACHUSETTS

In an embodiment a solvent composition can comprise, based on the total volume of the solvent composition, 10 to 95 volume percent of a first solvent, wherein the first solvent has Hansen solubility parameters of: 15 MPa0.5 ≤ δD ≤17 MPa0.5, 4 ≤ δP ≤ 10.5 MPa0.5, and 7 ≤ δH ≤ 10 MPa0.5; 5 to 95 volume percent of a second solvent, wherein the second solvent has Hansen solubility parameters of: 16 MPa0.5 ≤ δD ≤ 17.5 MPa0.5, 0 ≤ δΡ ≤ 3 MPa0.5, and 0 ≤ δΗ ≤ 3 MPa0.5; and 0 to 85 volume percent parachlorobenzotrifluoride; wherein the amounts of the first solvent, the second solvent, and parachlorobenzotrifluoride sum to at least 85 volume percent. The first solvent can comprise methyl acetate, acetone, dimethyl carbonate, ethyl acetate, n-butylamine, propyl acetate, tetrahydrofuran, or a combination thereof. The second solvent can comprise cyclohexene, cyclohexane, cyclopentane, methylcyclohexane, or a combination thereof. The solvent compositions are particularly useful in an adhesive composition. An adhesive composition includes the solvent composition, a rubber, and a tackifying resin. A method of bonding two surfaces is also described.

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Номер записи: 4