Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix

Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses a solid-phase extraction material that includes a hydrophilic solid support at least partially embedded within a hydrophobic matrix.

Compliant microfluidic sample processing device

Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well and one or more process chambers connected to the input well by one or more delivery channels. The process chambers may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

Sample processing devices and carriers

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.

Thermal structure for sample processing systems

Sample processing systems for processing sample materials located in sample processing devices that are separate from the system are disclosed. The sample processing systems include a rotating base plate with raised and/or non-planar thermal structures on which the sample processing devices are located during operation of the systems. The systems may also include structure to urge the sample processing devices against the base plate and thermal structures.

Biological sterilization indicator method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.

Methods and devices for removal of organic molecules from biological mixtures using anion exchange

Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.

Systems and methods for controlling humidity

Systems and methods for controlling humidity. Methods can include providing a chamber configured to receive objects to be sterilized; injecting a first quantity of water into the chamber; determining a pressure rise and a pressure drop in the chamber resulting from the first injection; calculating an absorption ratio (AR) as the ratio of the pressure drop to the pressure rise; sensing the humidity of the chamber after the first injection to determine a first humidity value (RHchamber); comparing the first humidity value to a pre-selected humidity value (RHsetpoint) to determine a humidity error (RHerror), wherein RHerror=RHsetpoint−RHchamber; and injecting a second quantity of water into the chamber, wherein the second quantity of water is calculated based on the absorption ratio (AR) and the humidity error (RHerror). Systems can perform the method; and the systems and methods can be employed in a sterilizer.

Sample processing devices

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

VARIABLE VALVE APPARATUS AND METHODS

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Sample processing device compression systems and methods

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

Modular sample processing apparatus kits and modules

Modular sample processing apparatus kits that can provide a user with the flexibility to customize a disk-based assay in view of a variety of factors are disclosed. The sample processing apparatus kits of the present invention include one or more process modules that can be retained within openings in a frame. The frame and process modules of the sample processing apparatus kits are preferably adapted for use in sample processing systems that compress the apparatus. The process modules may contain different reagents to perform different tests on the same sample materials or a variety of sample materials. As a result, a single sample processing apparatus can be used to perform a variety of different tests and may include a quality control module capable of providing feedback to the user as to the accuracy of the processes run using the sample processing apparatus. Methods of using the sample processing apparatus that include deforming the process modules and frame are also disclosed.

Methods and devices for removal of organic molecules from biological mixtures using anion exchange

Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.

ENHANCED SAMPLE PROCESSING DEVICES, SYSTEMS AND METHODS

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

COMPLIANT MICROFLUIDIC SAMPLE PROCESSING DEVICE

Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well and one or more process chambers connected to the input well by one or more delivery channels. The process chambers may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

Multi-format sample processing devices

Devices, methods, and systems for processing sample materials are disclosed. The present invention may provide a bridge between standard microtiter plate systems, methods, protocols, etc. (that include wells arranged in rectangular arrays) and rotating sample processing devices and systems that allow users to obtain the rapid processing advantages of the more advanced sample processing devices. The sample processing devices preferably include a rectangular body to improve compatibility of the sample processing devices of the present invention with equipment designed for use with more conventional microtiter plates (which are typically rectangular in shape). The sample processing devices also include at least one set of process chambers arranged in one or more circular arcs and may include input and/or output chambers arranged in a rectilinear grid array.

SYSTEMS AND METHODS FOR CONTROLLING HUMIDITY

Systems and methods for controlling humidity. Methods can include providing a chamber configured to receive objects to be sterilized; injecting a first quantity of water into the chamber; determining a pressure rise and a pressure drop in the chamber resulting from the first injection; calculating an absorption ratio (AR) as the ratio of the pressure drop to the pressure rise; sensing the humidity of the chamber after the first injection to determine a first humidity value (RH); comparing the first humidity value to a pre-selected humidity value (RH) to determine a humidity error (RH), wherein RH=RH−RH; and injecting a second quantity of water into the chamber, wherein the second quantity of water is calculated based on the absorption ratio (AR) and the humidity error (RH). Systems can perform the method; and the systems and methods can be employed in a sterilizer. 1. A method for controlling humidity , the method comprising:providing a chamber configured to receive objects to be sterilized;injecting a first quantity of water into the chamber as water vapor;determining a pressure rise and a pressure drop in the chamber resulting from injecting a first quantity of water into the chamber;calculating an absorption ratio (AR) as the ratio of the pressure drop to the pressure rise;{'sub': 'chamber', 'sensing the humidity of the chamber after injecting a first quantity of water to determine a first humidity value (RH);'}{'sub': setpoint', 'error', 'error', 'setpoint', 'chamber, 'comparing the first humidity value to a pre-selected humidity value (RH) to determine a humidity error (RH), wherein RH=RH−RH; and'}{'sub': 'error', 'injecting a second quantity of water into the chamber as water vapor, wherein the second quantity of water is calculated based on the absorption ratio (AR) and the humidity error (RH).'}2. The method of claim 1 , further comprising positioning a load of objects to be sterilized within the chamber and sealing the chamber.3. The method of claim 1 , ...

SAMPLE PROCESSING DEVICES

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

Biological sterilization indicator system and method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first unactivated position and a second activated position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.

Modular systems and methods for using sample processing devices

Sample processing systems and methods of using those systems for processing sample materials located in devices that are separate from the system are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include connection apparatus that allow for rapid and easy removal and/or replacement of base plates.

Sample processing devices

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

Systems for processing sample processing devices

A system and method for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The system can further include a cover including a first projection, and a housing. A portion of the housing can be movable with respect to the base plate between an open position and a closed position, and can include a second projection. The first projection and the second projection can be adapted to be coupled together when the portion is in the open position and decoupled when the portion is in the closed position. The method can include coupling the cover to the portion of the housing, moving the portion of the housing from the open position to the closed position, and rotating the base plate about the rotation axis.

SAMPLE MIXING ON A MICROFLUIDIC DEVICE

Mixing structures for use on sample processing devices are disclosed. The mixing structures include one or more mixing chambers in fluid communication with a process chamber, such that changing the rotational speed of the sample processing device forces sample material into and out of the mixing chamber to achieve mixing of the sample material. The mixing chambers are in fluid communication with the process chambers through mixing ports that are located on the distal sides of the process chambers with respect to the axis about which the sample processing device is rotated.

SYSTEMS FOR USING SAMPLE PROCESSING DEVICES

Sample processing systems for processing sample materials located in sample processing devices that are separate from the system are disclosed. The sample processing systems include a rotating base plate with raised and/or non-planar thermal structures on which the sample processing devices are located during operation of the systems. The systems may also include structure to urge the sample processing devices against the base plate and thermal structures.

Sample mixing on a microfluidic device

Mixing structures for use on sample processing devices are disclosed. The mixing structures include one or more mixing chambers in fluid communication with a process chamber, such that changing the rotational speed of the sample processing device forces sample material into and out of the mixing chamber to achieve mixing of the sample material. The mixing chambers are in fluid communication with the process chambers through mixing ports that are located on the distal sides of the process chambers with respect to the axis about which the sample processing device is rotated.

Systems for using sample processing devices

Sample processing systems for processing sample materials located in sample processing devices that are separate from the system are disclosed. The sample processing systems include a rotating base plate with raised and/or non-planar thermal structures on which the sample processing devices are located during operation of the systems. The systems may also include structure to urge the sample processing devices against the base plate and thermal structures.

Sample processing device having process chambers with bypass slots

Sample processing devices including process chambers having bypass slots and methods of using the same are disclosed. The bypass slots are formed in the sidewalls of the process chambers and are in fluid communication with distribution channels used to deliver fluid sample materials to the process chambers.

Sample processing device compression systems and methods

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

MULTIPLEX FLUORESCENCE DETECTION DEVICE HAVING REMOVABLE OPTICAL MODULES

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules. Each of the removable optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector.

Sample processing disk cover

BIOLOGICAL STERILIZATION INDICATOR SYSTEM AND METHOD

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.

Sample processing disk cover

Enhanced sample processing devices, systems and methods

Methods for processing sample materials. Methods of the present disclosure can include providing a device including a process chamber array, the process chamber array including a loading chamber and a process chamber; providing sample material in the process chamber array; moving the sample material within the process chamber array by rotating the device; providing paramagnetic particles within the sample material located in the process chamber array; providing a magnet proximate the device; and rotating the device such that the paramagnetic particles within the sample material are subjected to the magnetic field of the magnet during rotation of the device.

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Multiplex fluorescence detection device having removable optical modules

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules. Each of the removable optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector.

Sterilization Indicator Reading Apparatus with a Color Sensor

Aspects of the present disclosure relate to a reading apparatus that a well formed from a portion of a housing. The well dimensioned to receive at least a portion of a sterilization indicator having spores. The reading apparatus can also have a heating element thermally coupled to a portion of the well and an excitation source to excite the substance in the sterilization indicator. The reading apparatus can have a sterilization indicator activation circuit for detecting activation of the sterilization indicator. The excitation source is positioned such that light from the excitation source is directed into the well. A color sensor can be positioned adjacent the well to receive reflected light from the sterilization indicator.

Sample mixing on a microfluidic device

Mixing structures for use on sample processing devices are disclosed. The mixing structures include one or more mixing chambers in fluid communication with a process chamber, such that changing the rotational speed of the sample processing device forces sample material into and out of the mixing chamber to achieve mixing of the sample material. The mixing chambers are in fluid communication with the process chambers through mixing ports that are located on the distal sides of the process chambers with respect to the axis about which the sample processing device is rotated.

INTEGRATED SYSTEMS AND ASSEMBLIES FOR SAMPLE PROCESSING

Methods and devices for the thermal processing of samples are disclosed, including portable, integrated processing assemblies for occluding channels in a fluidic device. 1. A system for processing samples , the system comprising:a fluidic device comprising a deformable seal located along at least a portion of a process array; and 'an elongated housing having an open state and a closed state and including a base adapted to retain a fluidic device; and a slide housing operatively connected to the base at a first end and comprising a staking slide.', 'a sealing apparatus for closing the deformable seals in the fluidic device comprising;'}2. The system of claim 1 , further comprising one or more sealing structures attached to the staking slide claim 1 , the sealing structures facing the base claim 1 , wherein each sealing structure of the one or more sealing structures is adapted to deform at least a portion of the deformable seal.3. The system of claim 2 , wherein the staking slide is mounted for traversing movement across the slide housing claim 2 , and wherein the sealing structures are adapted to deform at least a portion of the deformable seal as the staking slide traverses the slide housing.4. The system of claim 1 , wherein the sealing structures are selected from a group consisting of blades claim 1 , styli claim 1 , pins claim 1 , and wires.5. The system of claim 1 , wherein the staking slide is enclosed within the slide housing.6. The system of claim 1 , wherein a heating element is coupled to the slide housing.7. The system of claim 6 , wherein the heating element comprises a thermally conductive material thermally coupled to the second side of the fluidic device.8. An integrated processing assembly for closing deformable seals in a fluidic device comprising:a base adapted to retain a fluidic device having a deformable seal;a slide housing operatively connected to the base and comprising a staking slide wherein the base and the slide housing define an ...

BIOLOGICAL STERILIZATION INDICATOR SYSTEM AND METHOD

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The reading apparatus can detect at least one of (i) when the well is empty; (ii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and (iii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position. The method can include detecting at least one of the above conditions, which can be used to detect an activation status of the biological sterilization indicator. 1. A biological sterilization indicator system , the system comprising: [ a first portion, and', 'a second portion adapted to be coupled to the first portion, the second portion being movable with respect to the first portion, when coupled to the first portion, between a first position and a second position; and, 'a housing including'}, 'a container containing a liquid and being dimensioned to be positioned in the housing, at least a portion of the container being frangible, the container positioned in at least the first portion of the housing, the container having a first state in which the container is intact when the second portion of the housing is in the first position, and a second state in which the container is fractured when the second portion of the housing is in the second position; and, 'a biological sterilization indicator comprising when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and', 'when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position., 'a reading apparatus comprising a well, the well ...

Sample processing devices

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

PROCESS CHALLENGE DEVICE AND METHODS

A process challenge device comprising process challenge device comprising a container defining a space within the container, wherein the space can fully contain a process indicator; and at least one pressure-actuating valve associated with the container, wherein the at least one pressure-actuating valve regulates entrance of a sterilant into and exiting of a gas and/or liquid out of the space within the container, methods of using the device and a kit including the device are disclosed.

Multi-format sample processing devices and systems

Devices, methods, and systems for processing sample materials are disclosed. The present invention may provide a bridge between standard microtiter plate systems, methods, protocols, etc. (that include wells arranged in rectangular arrays) and rotating sample processing devices and systems that allow users to obtain the rapid processing advantages of the more advanced sample processing devices. The sample processing devices preferably include a rectangular body to improve compatibility of the sample processing devices of the present invention with equipment designed for use with more conventional microtiter plates (which are typically rectangular in shape). The sample processing devices also include at least one set of process chambers arranged in one or more circular arcs and may include input and/or output chambers arranged in a rectilinear grid array.

Modular sample processing apparatus kits and modules

Modular sample processing apparatus kits that can provide a user with the flexibility to customize a disk-based assay in view of a variety of factors are disclosed. The sample processing apparatus kits of the present invention include one or more process modules that can be retained within openings in a frame. The frame and process modules of the sample processing apparatus kits are preferably adapted for use in sample processing systems that compress the apparatus. The process modules may contain different reagents to perform different tests on the same sample materials or a variety of sample materials. As a result, a single sample processing apparatus can be used to perform a variety of different tests and may include a quality control module capable of providing feedback to the user as to the accuracy of the processes run using the sample processing apparatus. Methods of using the sample processing apparatus that include deforming the process modules and frame are also disclosed.

Sterilizer with a humidity control system

The disclosed sterilizer includes a humidity control system for controlling humidity. The sterilizer for sterilizing objects comprises a chamber for receiving objects to be sterilized and a humidity control system. The humidity control system comprises a water control system which can be connected to a water source for injecting a first quantity of water vapor into the chamber, a pressure sensor in fluid communication with the chamber for measuring pressure in the chamber, a humidity sensor in fluid communication, or in selective fluid communication, with the chamber for sensing a first humidity value (RHchamber) of the first quantity of water vapor in the chamber; and a controller configured to inject a second quantity of water vapor into the chamber.

Sample processing devices and carriers

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.

Valve control system for a rotating multiplex fluorescence detection device

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Variable valve apparatus and methods

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

ANNULAR COMPRESSION SYSTEMS AND METHODS FOR SAMPLE PROCESSING DEVICES

Systems and methods for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The base plate can include at least one first magnetic element. The system can further include an annular cover, and a sample processing device comprising at least one thermal process chamber. The annular cover can include an inner edge, an outer edge, and at least one second magnetic element. The method can include positioning the sample processing device between the base plate and the annular cover, such that the inner edge of the annular cover is positioned inwardly of the at least one thermal process chamber, and such that the at least one first magnetic element attracts the at least one second magnetic element to force the annular cover in a first direction along the z-axis, urging the sample processing device into contact with the base plate.

SYSTEMS AND METHODS FOR PROCESSING SAMPLE PROCESSING DEVICES

A system and method for processing sample processing devices. The system can include a base plate adapted to rotate about a rotation axis. The system can further include a cover including a first projection, and a housing. A portion of the housing can be movable with respect to the base plate between an open position and a closed position, and can include a second projection. The first projection and the second projection can be adapted to be coupled together when the portion is in the open position and decoupled when the portion is in the closed position. The method can include coupling the cover to the portion of the housing, moving the portion of the housing from the open position to the closed position, and rotating the base plate about the rotation axis.

BIOLOGICAL STERILIZATION INDICATOR SYSTEM AND METHOD

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.

VALVE CONTROL SYSTEM FOR A ROTATING MULTIPLEX FLUORESCENCE DETECTION DEVICE

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Microbial indicator device for use with process monitoring systems

A microbial indicator device for liquid disinfection comprises a first cavity, a source of biological activity fluidically coupled to the first cavity via a portion of a first fluidic path, a filter membrane positioned in a second fluidic path for a disinfectant, and a first coupling portion fluidically coupled to the source of biologically activity via the second fluidic path. The filter membrane has a first side and a second side, wherein the first side is in fluid communication with the first fluidic path. The filter membrane has a pore size sufficient to retain at least a portion of the source of biological activity on the first side. The device further comprises a frangible container contained in the first cavity, wherein a liquid in the container is in fluid communication with the first cavity when the container is fractured.

SAMPLE PROCESSING DEVICE COMPRESSION SYSTEMS AND METHODS

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

ENHANCED SAMPLE PROCESSING DEVICES, SYSTEMS AND METHODS

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Biological sterilization indicator system and method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The reading apparatus can detect at least one of (i) when the well is empty; (ii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and (iii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position. The method can include detecting at least one of the above conditions, which can be used to detect an activation status of the biological sterilization indicator.

Sterilization process challenge device

A sterilization process challenge device comprising a solid body comprised of walls which define a space, wherein the space can fully contain a sterilization indicator and a volume of gas of at least 5 cubic centimeters and not more than 1000 cubic centimeters; a flow restrictor associated with the solid body such that any flow of the gas out of the space and any flow of a sterilant into the space is restricted; wherein the sterilization indicator indicates whether or not it has been contacted by the sterilant for a time and at a temperature sufficient to sterilize an article; and wherein the walls of the solid body are impervious to the sterilant and have a thickness of at least 0.3 centimeter, methods of using the device and a kit including the device are disclosed.

Process challenge device and methods

A process challenge device comprising process challenge device comprising a container defining a space within the container, wherein the space can fully contain a process indicator; and at least one pressure-actuating valve associated with the container, wherein the at least one pressure-actuating valve regulates entrance of a sterilant into and exiting of a gas and/or liquid out of the space within the container, methods of using the device and a kit including the device are disclosed.

Compliant microfluidic sample processing disks

Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well and one or more process chambers connected to the input well by one or more delivery channels. The process chambers may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

PROCESS CHALLENGE DEVICE AND METHOD

A sterilization process challenge device comprising a first container comprising walls which define a first space within the first container; a process indicator within the first space; at least one sterilant access for a sterilant to enter the first space within the first container; wherein at least one of the walls which defines the first space is a flexible wall; and wherein the walls are impervious to the sterilant; a method of using the device; and a kit including the device are disclosed.

Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system is described that includes a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of optical modules. Each of the optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of optical modules conveys the fluorescent light from the multiple optical modules to a single detector.

ENHANCED SAMPLE PROCESSING DEVICES, SYSTEMS AND METHODS

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

METHODS AND ARTICLES FOR SAMPLE PROCESSING

Methods and devices for the thermal processing of samples are disclosed, including sample processing devices featuring an overflow region for retaining excess fluid, as well as portable sealing apparatuses for occluding channels in a sample processing device. 1. A sample processing device comprising: a loading structure;', 'a main conduit comprising a length;', 'a plurality of process chambers distributed adjacent to the main conduit, wherein the loading structure is in fluid communication with the plurality of process chambers through the main conduit;', 'a deformable seal; and', 'an overflow region having capacity to retain a volume of at least the excess volume., 'a body that comprises a first side attached to a second side, and one or more process arrays formed between the first and second sides, wherein each process array of the one or more process arrays includes2. The sample processing device of claim 1 , wherein the overflow region is located between the loading structure and the plurality of process chambers.3. The sample processing device of claim 2 , wherein the overflow region comprises at least one reservoir adjacent to the main conduit and in fluid communication with the main conduit.4. The sample processing device of claim 3 , wherein the reservoir is configured to be isolated from the main conduit and process chambers when the main conduit is occluded.5. The sample processing device of claim 3 , wherein the reservoir comprises a distal edge portion claim 3 , the edge portion forming a reservoir offset angle with the main conduit claim 3 , and wherein the reservoir offset angle is at least 90 degrees.6. The sample processing device of claim 5 , wherein the reservoir offset angle is at least 120 degrees.7. The sample processing device of claim 1 , wherein the overflow region comprises a portion of the main conduit.8. The sample processing device of wherein the main conduit comprises a central axis and wherein the overflow region comprises a portion of ...

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Sample processing apparatus, methods and systems

Apparatus, methods, and systems for processing sample materials that may be presented in a standard microtiter plate are disclosed. The present invention provides a bridge between the standard microtiter plate systems, methods, protocols, etc. with their stationary wells and rotating sample processing devices that allow users to obtain the rapid processing advantages of the newer sample processing devices while retaining the benefits of the standard microtiter plate formats.

Sample processing devices

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

Valve control system for a rotating multiplex fluorescence detection device

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials.

Sample processing disk cover

Rotatable sample processing disk

Variable valve apparatus and methods

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Sterilizer with a humidity control system

The disclosed sterilizer includes a humidity control system for controlling humidity. The sterilizer for sterilizing objects comprises a chamber for receiving objects to be sterilized and a humidity control system. The humidity control system comprises a water control system which can be connected to a water source for injecting a first quantity of water vapor into the chamber, a pressure sensor in fluid communication with the chamber for measuring pressure in the chamber, a humidity sensor in fluid communication, or in selective fluid communication, with the chamber for sensing a first humidity value (RHchamber) of the first quantity of water vapor in the chamber; and a controller configured to inject a second quantity of water vapor into the chamber.

READERS FOR PROCESS MONITORING SYSTEMS AND METHODS OF USE

Process monitoring systems include a reader having a read sensor, an optional reference sensor, and a light source. The reader optionally includes an optical block defining an interior volume and an aperture aligned with the read sensor to allow diffuse light to be received by the read sensor. One or more percent reflectance values may be calculated in response to a dark measurement, a correction function, and normalization. The one or more percent reflectance values may be compared to a threshold to determine whether a disinfection cycle was effective. The reader may indicate the determination to a user and provide the determination to a tracking system for diagnostics, inventory management, or compliance monitoring. 1. A system comprising: an optical block defining an interior volume and an aperture, the axis extending through the aperture when the process indicator is in the reading position;', 'a light source disposed in the interior volume and configured to illuminate the indicator surface with light, the axis not extending through the light source when the process indicator is in the reading position;', 'optionally, a reference sensor positioned in the interior volume to receive, at a reference sensor surface, light from the light source that has not passed through the aperture, the reference sensor configured to provide a reference sensor signal value representing an intensity of light received, the axis not extending through the reference sensor surface when the process indicator is in the reading position;', 'a read sensor disposed in the interior volume to receive, at a read sensor surface, light from the light source that has reflected off the indicator surface and has passed through the aperture, the read sensor configured to provide a read sensor signal value representing an intensity of reflected light received; and, 'a reader configured to indicate whether an indicator surface of a process indicator has a color representative of an effective ...

REMOVABLE CARTRIDGES FOR USE WITH PROCESS MONITORING SYSTEMS, AND SYSTEMS COMPRISING SAME

A cartridge for use with a process monitoring system. The process monitoring system can include an adapter comprising a first fluid pathway and configured to be positioned in fluid communication with a reprocessing system. At least a portion of the cartridge can be configured to be removably received in a receptacle of the adapter. The cartridge can include a second fluid pathway configured to be positioned in fluid communication with the first fluid pathway of the adapter when at least a portion of the cartridge is positioned in the receptacle of the adapter. The cartridge can further include at least one indicator positioned on the cartridge in fluid communication with the second fluid pathway of the cartridge. 1. A cartridge for use with a process monitoring system , the process monitoring system comprising an adapter comprising a first fluid pathway and configured to be positioned in fluid communication with a reprocessing system , at least a portion of the cartridge configured to be removably received in a receptacle of the adapter , the cartridge comprising:a second fluid pathway having an inlet and an outlet configured to be positioned in fluid communication with the first fluid pathway of the adapter when at least a portion of the cartridge is positioned in the receptacle of the adapter, such that the second fluid pathway of the cartridge is in fluid communication with the first fluid pathway of the adapter and fluid flow through the first fluid pathway of the adapter is at least partially diverted through the second fluid pathway of the cartridge when at least a portion of the cartridge is received in the receptacle of the adapter, andat least one indicator positioned on the cartridge in fluid communication with the second fluid pathway of the cartridge.2. (canceled)3. The cartridge of claim 1 , wherein at least one of the first fluid pathway and the second fluid pathway includes a shunt channel.4. The cartridge of claim 1 , wherein at least one of the ...

PROCESS CHALLENGE DEVICE AND METHODS

A process challenge device comprising process challenge device comprising a container defining a space within the container, wherein the space can fully contain a process indicator; and at least one pressure-actuating valve associated with the container, wherein the at least one pressure-actuating valve regulates entrance of a sterilant into and exiting of a gas and/or liquid out of the space within the container, methods of using the device and a kit including the device are disclosed. 1. A method of determining effectiveness of a sterilization process , the method comprising: a container defining a space within the container, wherein the space contains a process indicator; and', 'at least one pressure-actuating valve associated with the container, wherein the at least one pressure-actuating valve regulates entrance of a sterilant into and exiting of a gas or liquid out of the space within the container;, 'providing a process challenge device comprisingpositioning the process challenge device in a sterilization chamber; andexposing the process challenge device to the sterilant at an elevated temperature.2. The method of claim 1 , further comprising determining whether or not the process indicator indicates that it has been exposed to sterilization process conditions effective for sterilizing an article.3. The method of claim 1 , wherein the at least one pressure-actuating valve is actuated when there is a pressure difference between the space within the container and outside of the first container.4. The method of claim 3 , wherein at least one pressure-actuated valve can be adjusted to increase or decrease the pressure difference required to actuate the valve.5. The method of claim 4 , wherein the pressure difference required is at least 6.895 kPa (1 psi).6. The method of claim 4 , further comprising adjusting the at least one pressure-actuated valve of the process challenge device to provide a targeted level of resistance to the sterilization process.7. The ...

MICROBIAL INDICATOR DEVICE FOR USE WITH PROCESS MONITORING SYSTEMS

A microbial indicator device for liquid disinfection comprises a first cavity, a source of biological activity fluidically coupled to the first cavity via a portion of a first fluidic path, a filter membrane positioned in a second fluidic path for a disinfectant, and a first coupling portion fluidically coupled to the source of biologically activity via the second fluidic path. The filter membrane has a first side and a second side, wherein the first side is in fluid communication with the first fluidic path. The filter membrane has a pore size sufficient to retain at least a portion of the source of biological activity on the first side. The device further comprises a frangible container contained in the first cavity, wherein a liquid in the container is in fluid communication with the first cavity when the container is fractured. 1. A microbial indicator device for liquid disinfection , comprising: a first cavity;', 'a source of biological activity, wherein the source of biological activity is fluidically coupled to the first cavity via a portion of a first fluidic path;', 'a first coupling portion having a first channel extending longitudinally therethrough that defines a portion of a second fluidic path, wherein the first coupling portion is fluidically coupled to the source of biologically activity via the second fluidic path;, 'a first housing comprising 'wherein the filter membrane has effective porosity sufficient to retain at least a portion of the source of biological activity on the first side.', 'a filter membrane positioned in the second fluidic path, wherein the filter membrane has a first side and a second side, wherein the second fluidic path is established between the first coupling portion and the second side,'}2. The microbial indicator device of claim 1 , wherein the filter membrane has a filter membrane surface area and the first coupling portion has an inlet area partially defined by a cross-section of the first channel claim 1 , wherein a ...

Sample processing device with optical elements

Apparatus, methods, and systems for sample processing utilizing an optical element to open and/or close a valve.

Bone stapler cartridge

A cartridge for use in a stapler for driving staples during orthopedic surgery is described. The cartridge has a case comprising a first wall and side walls projecting from this first wall. The cartridge has inner guide surfaces intended to guide the movement of staples when they are ejected by the stapler, and these preferably include the top of extended projections from a spacer element which is snap fit to the case. Within the case are a stack of staples supported on a follower which pushes on the side of the stack of staples so as to sequentially present each one in turn in a position to be ejected by the stapler. The follower is centered and slidably mounted on a guidepost which helps the follower resist any tendency for misalignment of the follower within the case.

Multiplex fluorescence detection device having removable optical modules.

Se describen tecnicas para la deteccion de especies objetivo multiples en la PCR (reaccion en cadena de la polimerasa) en tiempo real. Por ejemplo, un sistema comprende un dispositivo de adquisicion de datos y un dispositivo de deteccion acoplado al dispositivos de adquisicion de datos. El dispositivo de deteccion incluye un disco giratorio que tiene una pluralidad de camaras de proceso que tienen una pluralidad de especies que emiten luz fluorescente a diferentes longitudes de onda. El dispositivos incluye ademas una pluralidad de modulos opticos removibles. Cada uno de los modulos opticos removibles esta configurado opticamente para excitar las especies y para capturar la luz fluorescente emitida por las especies a diferentes longitudes de onda. Un haz de fibras opticas acoplado a la pluralidad de modulos opticos removibles transporta la luz fluorescente desde los modulos opticos hasta un solo detector.

Compliant microfluidic sample processing disks

Microfluidic sample processing disks with a plurality of fluid structures formed therein are disclosed. Each of the fluid structures preferably includes an input well (20) and one or more process chambers (30) connected to the input well (20) by one or more delivery channels (42, 44, 46). The process chambers (30) may be arranged in a compliant annular processing ring that is adapted to conform to the shape of an underlying thermal transfer surface under pressure. That compliance may be delivered in the disks of the present invention by locating the process chambers in an annular processing ring in which a majority of the volume is occupied by the process chambers. Compliance within the annular processing ring may alternatively be provided by a composite structure within the annular processing ring that includes covers attached to a body using pressure sensitive adhesive.

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is rotated during heating of the sample materials. The device may comprise a valve in a channel comprising an impermeable membrane that can be opened by forming a void in said membrane using electromagnetic energy.

Sample processing devices

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The sample processing devices provide process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas.

Sample processing apparatus, methods and systems

Apparatus, methods, and systems for processing sample materials that may be presented in a standard microtiter plate are disclosed. The present invention provides a bridge between the standard microtiter plate systems, methods, protocols, etc. with their stationary wells and rotating sample processing devices that allow users to obtain the rapid processing advantages of the newer sample processing devices while retaining the benefits of the standard microtiter plate formats.

Multiplex fluorescence detection device having removable optical modules

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules. Each of the removable optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector.

sterilization biological indicator method and system

MÉTODO E SISTEMA DE INDICADOR BIOLÓGICO DE ESTERILIZAÇÃO. A presente invenção refere-se a um sistema e a um método de indicador biológico de esterilização (IB). O sistema pode incluir um IB e um aparelho de leitura que compreende uma cavidade. O IB pode incluir um compartimento, que pode incluir uma primeira porção e uma segunda porção móveis entre uma primeira posição "não ativada" e uma segunda posição "ativada". O aparelho de leitura configurado para detectar ao menos uma das seguintes condições (i) quando a cavidade está vazia; (ii) quando o indicador biológico de esterilização é posicionado na cavidade com a segunda porção do compartimento na primeira posição e (iii) quando o indicador biológico de esterilização é posicionado na cavidade com a segunda porção do compartimento na segunda posição. O método pode incluir detectar ao menos uma das condições acima, que podem ser usadas para detectar um estado de ativação do indicador biológico de esterilização. METHOD AND SYSTEM OF BIOLOGICAL STERILIZATION INDICATOR. The present invention relates to a system and a method of biological sterilization indicator (IB). The system can include an IB and a reading device that comprises a cavity. The IB may include a compartment, which may include a first movable portion and a second portion between a first "not activated" position and a second "activated" position. The reading apparatus configured to detect at least one of the following conditions (i) when the cavity is empty; (ii) when the biological sterilization indicator is positioned in the cavity with the second portion of the compartment in the first position and (iii) when the biological sterilization indicator is positioned in the cavity with the second portion of the compartment in the second position. The method may include detecting at least one of the above conditions, which can be used to detect an activated state of the biological sterilization indicator.

Removable cartridges for use with process monitoring systems, and systems comprising same

A cartridge for use with a process monitoring system. The process monitoring system can include an adapter comprising a first fluid pathway and configured to be positioned in fluid communication with a reprocessing system. At least a portion of the cartridge can be configured to be removably received in a receptacle of the adapter. The cartridge can include a second fluid pathway configured to be positioned in fluid communication with the first fluid pathway of the adapter when at least a portion of the cartridge is positioned in the receptacle of the adapter. The cartridge can further include at least one indicator positioned on the cartridge in fluid communication with the second fluid pathway of the cartridge.

Sample processing devices and carriers

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group pf process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exiting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.

Biological sterilization indicator system and method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first "unactivated" position and a second "activated" position. The reading apparatus can detect at least one of (i) when the well is empty; (ii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and (iii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position. The method can include detecting at least one of the above conditions, which can be used to detect an activation status of the biological sterilization indicator.

Biological sterilization indicator system and method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first "unactivated" position and a second "activated" position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions.

Sample mixing on a microfluidic device

Mixing structures for use on sample processing devices are disclosed. The mixing structures include one or more mixing chambers (60) in fluid communication with a process chamber (40), such that changing the rotational speed of the sample processing device forces sample material into and out of the mixing chamber to achieve mixing of the sample material. The mixing chambers are in fluid communication with the process chambers through mixing ports (62) that are located on the distal sides of the process chambers with respect to the axis about which the sample processing device is rotated.

Sample mixing on a microfluidic device

Modular systems and methods for using sample processing devices

Sample processing systems and methods of using those systems for processing sample materials located in devices that are separate from the system are disclosed. The sample processing systems include a rotating base (10) on which the sample processing devices are located during operation of the systems. The systems also include connection apparatus that allow for rapid and easy removal and/or replacement of base plates.

DEVICE, METHOD AND USE FOR TREATING SAMPLES

Sample processing device with compression systems and method of using same

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

Sterilization process challenge device and method

A sterilization process challenge device comprising a solid body comprised of walls which define a space, wherein the space can fully contain a sterilization indicator and a volume of gas of at least 5 cubic centimeters and not more than 1000 cubic centimeters; a flow restrictor associated with the solid body such that any flow of the gas out of the space and any flow of a sterilant into the space is restricted; wherein the sterilization indicator indicates whether or not it has been contacted by the sterilant for a time and at a temperature sufficient to sterilize an article; and wherein the walls of the solid body are impervious to the sterilant and have a thickness of at least 0.3 centimeter, methods of using the device and a kit including the device are disclosed.

Biological sterilization indicator system and method

A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first "unactivated" position and a second "activated" position. The reading apparatus can detect at least one of (i) when the well is empty; (ii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the first position, and (iii) when the biological sterilization indicator is positioned in the well with the second portion of the housing in the second position. The method can include detecting at least one of the above conditions, which can be used to detect an activation status of the biological sterilization indicator.

Sample processing device with compression systems and method of using same

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

Variable valve apparatus and methods

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system is described that includes a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of optical modules. Each of the optical modules is optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of optical modules conveys the fluorescent light from the multiple optical modules to a single detector.

Sterilization process challenge device and method

A sterilization process challenge device comprising a solid body comprised of walls which define a space, wherein the space can fully contain a sterilization indicator and a volume of gas of at least 5 cubic centimeters and not more than 1000 cubic centimeters; a flow restrictor associated with the solid body such that any flow of the gas out of the space and any flow of a sterilant into the space is restricted; wherein the sterilization indicator indicates whether or not it has been contacted by the sterilant for a time and at a temperature sufficient to sterilize an article; and wherein the walls of the solid body are impervious to the sterilant and have a thickness of at least 0.3 centimeter, methods of using the device and a kit including the device are disclosed.

Methods and devices for removal of organic molecules from biological mixtures using anion exchange

Methods and devices (10) for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.

Heating element for a rotating multiplex fluorescence detection device

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. The device further includes a heating element for heating one or more process chambers on the disk. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Variable valve apparatus and methods

Sample processing devices with variable valve structures and methods of using the same are disclosed. The valve structures allow for removal of selected portions of the sample material located within the process chamber. Removal of the selected portions is achieved by forming an opening in a valve septum at a desired location. The valve septums may be large enough to allow for adjustment of the location of the opening based on the characteristics of the sample material in the process chamber. If the sample processing device is rotated after the opening is formed, the selected portion of the material located closer to the axis of rotation exits the process chamber through the opening. The remainder of the sample material cannot exit through the opening because it is located farther from the axis of rotation than the opening.

Sample processing devices and methods

Methods and devices for thermal processing of multiple samples at the same time are disclosed. The assemblies include carriers and sample processing devices with process arrays that include conduits useful in distributing sample materials to a group of process chambers located in fluid communication with the main conduits. The sample processing devices may include one or more of the following features in various combinations: deformable seals, process chambers connected to the main conduit by feeder conduits exciting the main conduit at offset locations, U-shaped loading chambers, and a combination of melt bonded and adhesively bonded areas. The carriers may be used to apply selective compression to the sample processing devices.

Microbial indicator device for use with process monitoring systems

A microbial indicator device for liquid disinfection comprises a first cavity, a source of biological activity fluidically coupled to the first cavity via a portion of a first fluidic path, a filter membrane positioned in a second fluidic path for a disinfectant, and a first coupling portion fluidically coupled to the source of biologically activity via the second fluidic path. The filter membrane has a first side and a second side, wherein the first side is in fluid communication with the first fluidic path. The filter membrane has a pore size sufficient to retain at least a portion of the source of biological activity on the first side. The device further comprises a frangible container contained in the first cavity, wherein a liquid in the container is in fluid communication with the first cavity when the container is fractured.

Compliant microfluidic sample processing disks

Sample mixing on a microfluidic device

Process challenge device and method

A sterilization process challenge device comprising a first container comprising walls which define a first space within the first container; a process indicator within the first space; at least one sterilant access for a sterilant to enter the first space within the first container; wherein at least one of the walls which defines the first space is a flexible wall; and wherein the walls are impervious to the sterilant; a method of using the device; and a kit including the device are disclosed.

Sample processing device with compression systems and method of using same

Sample processing systems and methods of using those systems for processing sample materials located in sample processing devices are disclosed. The sample processing systems include a rotating base plate on which the sample processing devices are located during operation of the systems. The systems also include a cover and compression structure designed to force a sample processing device towards the base plate. The preferred result is that the sample processing device is forced into contact with a thermal structure on the base plate. The systems and methods of the present invention may include one or more of the following features to enhance thermal coupling between the thermal structure and the sample processing device: a shaped transfer surface, magnetic compression structure, and floating or resiliently mounted thermal structure. The methods may preferably involve deformation of a portion of a sample processing device to conform to a shaped transfer surface.

detection device, detection system, and method

dispositivo de detecção, sistema de detecção, e, método. são descritas técnicas para a detecção de múltiplas espécies- alvo em pcr em tempo real (reação em cadeia da polimerase). por exemplo, um sistema é descrito, que inclui um dispositivo de aquisição de dado e um dispositivo de detecção acoplado com o dispositivo de aquisição de dado. um dispositivo de detecção inclui um disco rotativo tendo uma pluralidade de câmaras de processo tendo uma pluralidade de espécies que emitem luz fluorescente em diferentes comprimentos de onda. o dispositivo também inclui uma pluralidade de módulos ópticos. cada um dos módulos ópticos é opticamente configurado para excitar as espécies e capturar luz fluorescente emitida pelas espécies em diferentes comprimentos de onda. um feixe de fibras ópticas acoplado com a pluralidade de módulos ópticos transmite a luz fluorescente dos múltiplos módulos ópticos para um único detector. detection device, detection system, and method. Techniques for the detection of multiple target species in pcr in real time (polymerase chain reaction) are described. For example, a system is described which includes a data acquisition device and a detection device coupled with the data acquisition device. A detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device also includes a plurality of optical modules. Each of the optical modules is optically configured to excite species and capture fluorescent light emitted by species at different wavelengths. An optical fiber bundle coupled with a plurality of optical modules transmits fluorescent light from multiple optical modules to a single detector.

Biological sterilization indicator system and method

Un sistema (10) indicador de esterilización biológico, comprendiendo el sistema: un indicador (100) de esterilización biológico que comprende: un bastidor (102) que incluye una primera parte (104), y una segunda parte (106) adaptada para acoplarse a la primera parte (104), siendo la segunda parte (106) móvil con respecto a la primera parte (104), cuando se acopla a la primera parte (104), entre una primera posición y una segunda posición; y un recipiente (120) que contiene un líquido (122) y que se dimensiona para colocarse en el bastidor (102), siendo al menos una parte del recipiente (120) frangible, teniendo el recipiente (120) un primer estado en el que el recipiente (120) está intacto cuando la segunda parte (106) del bastidor (102) está en la primera posición, y un segundo estado en el que el recipiente (120) está fracturado cuando la segunda parte (106) del bastidor (102) está en la segunda posición; una primera cámara (109) en el interior del bastidor (102) en la que el recipiente (120) se coloca cuando el recipiente (120) está en el primer estado; y una segunda cámara (111) dentro del bastidor (102) en la que el recipiente (120) y el líquido (122) no están colocados cuando el recipiente (120) está en el primer estado, y a cuyo interior se desplaza el líquido (122) cuando el recipiente (120) está en el segundo estado, comprendiendo la segunda cámara (111) al menos una fuente de actividad biológica (115) que no está en comunicación de fluidos con el líquido (122) cuando el recipiente (120) está en el primer estado y que está en comunicación de fluidos con el líquido (122) cuando el recipiente (120) está en el segundo estado; y un aparato (12) de lectura que comprende un pocillo (14), dimensionado el pocillo (14) para recibir al menos una parte del indicador (100) de esterilización biológico, en donde el aparato (12) de lectura incluye además un primer sensor (52) colocado para detectar la posición de la segunda parte (106) del bastidor (102). A biological ...

Enhanced sample processing devices, systems and methods

Enhanced sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is in the form of a disk, rotated during heating of the sample materials. The temperature of the disk may be controlled by directing electromagnetic energy at its bottom surface while rotating it. Sample materials may be moved betwwen process chambers using centrifugal force.

Methods and devices for removal of organic molecules from biological mixtures using anion exchange

Methods and devices (10) for removing small negatively charged molecules from a biological sample mixture that uses an anion exchange material.

Heating element for a rotating multiplex fluorescence detection device

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. The device further includes a heating element for heating one or more process chambers on the disk. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

sterilization biological indicator system and method for detecting an activation state of a sterilization biological indicator

método e sistema de indicador biológico de esterllização a presente invenção refere-se a um sistema e a um método de indicador biológico de esterilização (ib). o sistema pode incluir um ib e um aparelho de leitura que compreende uma cavidade. o ib pode incluir um compartimento, que pode incluir uma primeira porção e uma segunda porção móveis entre uma primeira posição "não ativada" e uma segunda posição "ativada". o ib pode incluir adicionalmente um recipiente frangível que contém um líquido e ser dimensionado para ser posicionado no compartimento. o aparelho de leitura pode ser configurado para detectar a ativação do indicador biológico de esterilização, por exemplo, através da detecção de que a segunda porção está na segunda posição, e/ou através da detecção de que o líquido do recipiente frangível está presente em uma câmara específica do indicador biológico de esterilização. o método pode incluir posicionar o ib na cavidade do aparelho de leitura e detectar a ativação, por exemplo, através da detecção de uma ou mais das condições acima. The present invention relates to a sterilization biological indicator system and method (ib). the system may include an ib and a reading apparatus comprising a cavity. the ib may include a compartment, which may include a first portion and a second portion movable between a first "unactivated" position and a second "activated" position. the ib may additionally include a frangible container which contains a liquid and be sized to be positioned in the compartment. the reading device can be configured to detect the activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid in the frangible container is present in a specific chamber of the biological sterilization indicator. the method may include positioning the ib in the readout cavity and detecting activation, for example, by detecting one or ...

Modular sample processing apparatus kits and modules

Modular sample processing apparatus kits that can provide a user with the flexibility to customize a disk-based assay in view of a variety of factors are disclosed. The sample processing apparatus kits of the present invention include one or more process modules that can be retained within openings in a frame. The frame and process modules of the sample processing apparatus kits are preferably adapted for use in sample processing systems that compress the apparatus. The process modules may contain different reagents to perform different tests on the same sample materials or a variety of sample materials. As a result, a single sample processing apparatus can be used to perform a variety of different tests and may include a quality control module capable of providing feedback to the user as to the accuracy of the processes run using the sample processing apparatus. Methods of using the sample processing apparatus that include deforming the process modules and frame are also disclosed.

Multiplex-fluoreszenzdetektionsvorrichtung mit entfernbaren optischen modulen

Verfahren und vorrichtungen zur entfernung von organischen molekühlen aus biologischen gemischen unter verwendung von anionenaustausch

Probenmischen auf einer mikrofluidischen vorrichtung und verfahren

Multiformat-probenprozessierungsvorrichtungen, - verfahren und -systeme

Multiplex fluorescence detection device having removable optical modules

Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector

Valve control system for a rotating multiplex fluorescence detection device

Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a plurality of species that emit fluorescent light at different wavelengths. The device further includes a plurality of removable optical modules that are optically configured to excite the species and capture fluorescent light emitted by the species at different wavelengths. A fiber optic bundle coupled to the plurality of removable optical modules conveys the fluorescent light from the optical modules to a single detector. In addition, the device may control the flow of fluid in the disk by locating and selectively opening valves separating chambers by heating the valves with a laser.

Valve control system for a rotating multiplex fluoresence detection device

Dispositivo de detección, sistema de detección y método

Dispositivo de detección (10) que comprende: un disco (13) que tiene una cámara acumuladora (125) separada de una cámara de proceso (129) por medio de una válvula (127); un motor (126) para hacer girar el disco (13), y una única fuente de energía (51) configurada para producir energía electromagnética en un primer nivel de energía para determinar una posición del disco (13) y un segundo nivel de energía para abrir la válvula (127) para permitir que fluya fluido de la cámara acumuladora (125) a la cámara de proceso (129), en donde el primer nivel de energía es diferente del segundo nivel de energía.

Systems and methods for controlling humidity

Systems and methods for controlling humidity. Methods can include providing a chamber configured to receive objects to be sterilized; injecting a first quantity of water into the chamber; determining a pressure rise and a pressure drop in the chamber resulting from the first injection; calculating an absorption ratio ( AR ) as the ratio of the pressure drop to the pressure rise; sensing the humidity of the chamber after the first injection to determine a first humidity value ( RH chamber ); comparing the first humidity value to a pre-selected humidity value ( RH setpoint ) to determine a humidity error ( RH error ), wherein RH error = RH setpoint - RH chamber ; and injecting a second quantity of water into the chamber, wherein the second quantity of water is calculated based on the absorption ratio ( AR ) and the humidity error ( RH error ). Systems can perform the method; and the systems and methods can be employed in a sterilizer.

Systems and methods for controlling humidity

Systems and methods for controlling humidity. Methods can include providing a chamber configured to receive objects to be sterilized; injecting a first quantity of water into the chamber; determining a pressure rise and a pressure drop in the chamber resulting from the first injection; calculating an absorption ratio ( AR ) as the ratio of the pressure drop to the pressure rise; sensing the humidity of the chamber after the first injection to determine a first humidity value ( RH chamber ); comparing the first humidity value to a pre-selected humidity value ( RH setpoint ) to determine a humidity error ( RH error ), wherein RH error = RH setpoint - RH chamber ; and injecting a second quantity of water into the chamber, wherein the second quantity of water is calculated based on the absorption ratio ( AR ) and the humidity error ( RH error ). Systems can perform the method; and the systems and methods can be employed in a sterilizer.

Systems and methods for controlling humidity

Método para o controle de umidade, sistema de controle de umidade para manipular o ambiente gasoso no interior de uma câmara e esterilizador para esterilizar objetos com gás biocida

SISTEMAS E MÉTODOS PARA O CONTROLE DE UMIDADE A presente invenção se refere a sistemas e métodos para o controle de umidade. Os métodos podem incluir o fornecimento de um a câmara configurada para receber objetos a serem esterilizados; injetar uma primeira quantidade de água para dentro da câmara; determinar um aumento de pressão e uma queda de pressão na câmara resultante da primeira injeção; calcular uma razão de absorção ( RA ) como a razão da queda de pressão em relação ao aumento de pressão; detectar a umidade da câmara após a primeira injeção para determinar um primeiro valor de umidade (UR câmara); comparar o primeiro valor de umidade com um valor de umidade pré-selecionado (UR ponto de ajuste) para determinar um erro de umidade (UR erro), em que URerro = UR ponto de ajuste - UR câmara; e injetar uma segunda quantidade de água para dentro da câmara, sendo que a segunda quantidade de água é calculada com base na razão de absorção (RA) e no erro de umidade (UR erro). Sistemas podem executar o método; e os sistemas e métodos podem ser empregados em um esterilizador.

Sample processing devices, systems and methods

Devices, systems, and methods for processing sample materials. The sample materials may be located in a plurality of process chambers in the device, which is in the form of a disk, rotated during heating of the sample materials. The temperature of the disk may be controlled by directing electromagnetic energy at its bottom surface while rotating it. Sample materials may be moved betwwen process chambers using centrifugal force.

Systems and methods for processing sample processing devices

The system can include a base plate adapted to rotate about a rotation axis. The system can further include a cover including a first projection, and a housing. A portion of the housing can be movable with respect to the base plate between an open position and a closed position, and can include a second projection. The first projection and the second projection can be adapted to be coupled together when the portion is in the open position and decoupled when the portion is in the closed position. The method can include coupling the cover to the portion of the housing, moving the portion of the housing from the open position to the closed position, and rotating the base plate about the rotation axis.

Modular sample processing apparatus kits and modules

Modular sample processing apparatus kits that can provide a user with the flexibility to customize a disk-based assay in view of a variety of factors are disclosed. The sample processing apparatus kits of the present invention include one or more process modules that can be retained within openings in a frame. The frame and process modules of the sample processing apparatus kits are preferably adapted for use in sample processing systems that compress the apparatus. The process modules may contain different reagents to perform different tests on the same sample materials or a variety of sample materials. As a result, a single sample processing apparatus can be used to perform a variety of different tests and may include a quality control module capable of providing feedback to the user as to the accuracy of the processes run using the sample processing apparatus. Methods of using the sample processing apparatus that include deforming the process modules and frame are also disclosed.

Modular sample processing apparatus kits and modules

Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix

Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix

Methods and devices for removing small negatively charged molecules from a biological sample mixture that uses a solid-phase extraction material that includes a hydrophilic solid support at least partially embedded within a hydrophobic matrix.