30-06-2016 дата публикации
Номер: US20160183561A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to isolated polypeptides having α-L-galactosidase activity, catalytic domains and poly-nucleotides encoding the polypeptides andcatalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains. The α-L-galactosidase can release L-galactose from corn fibers and corn xylan. 159-. (canceled)60. A variant polypeptide having α-L-galactosidase activity and having at least 80% sequence identity to SEQ ID NO: 2 , SEQ ID NO: 3 , SEQ ID NO: 4 , SEQ ID NO: 5 or SEQ ID NO: 6 comprising at least one substitution , deletion , and/or insertion of at least one or more amino acids of SEQ ID NO: 2 , SEQ ID NO: 3 , SEQ ID NO: 4 , SEQ ID NO: 5 or SEQ ID NO: 6.61. The variant polypeptide of and having at least 85% sequence identity to SEQ ID NO: 2 claim 60 , SEQ ID NO: 3 claim 60 , SEQ ID NO: 4 claim 60 , SEQ ID NO: 5 or SEQ ID NO: 6.62. The variant polypeptide of and having at least 90% sequence identity to SEQ ID NO: 2 claim 60 , SEQ ID NO: 3 claim 60 , SEQ ID NO: 4 claim 60 , SEQ ID NO: 5 or SEQ ID NO: 6.63. The variant polypeptide of and having at least 95% sequence identity to SEQ ID NO: 2 claim 60 , SEQ ID NO: 3 claim 60 , SEQ ID NO: 4 claim 60 , SEQ ID NO: 5 or SEQ ID NO: 6.64. The variant polypeptide of claim 60 , wherein the catalytic domain is obtained from an α-L-galactosidase.65. A composition comprising a variant polypeptide of .66. A polynucleotide encoding a variant polypeptide of claim 60 , provided that it is not 100% identical to SEQ ID NO: 1 or the mature polypeptide coding part thereof.67. A nucleic acid construct or expression vector comprising the polynucleotide of operably linked to one or more control sequences that direct the production of the variant polypeptide in an expression host.68. A recombinant host cell comprising the polynucleotide of operably linked to one or more control sequences ...
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