16-04-2015 дата публикации
Номер: US20150105343A1
Принадлежит:
In various embodiments, a cell culture medium, or a nucleoside cocktail transmission (NCT) medium for the culture of stem cells with improved genetic stability, cellular function and regeneration ability is provided. Illustrative culture media comprise a basal culture medium for stem cells, where the culture medium is supplemented with one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. Illustrative NCT media comprise a delivery vehicle (e g. skin creams or other vehicle) containing one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. The NCT medium provides, inter alia, direct delivery of nucleoside cocktails into human tissues (such as skin) for regenerative, cosmetic and/or therapeutic purposes. 1. A cell culture medium for the culture of stem cells with improved genetic stability , said culture medium comprising:a basal culture medium for stem cells, where said culture medium is supplemented with one or more nucleoside triphosphates or one or more precursors thereof.2. A nucleoside cocktail transmission (NCT) medium for improving somatic or stem cell genetic stability said medium comprising:a cosmetic or pharmaceutical delivery vehicle; andone or more nucleoside triphosphates or precursors thereof.3. The cell culture medium of claim 1 , wherein said one or more nucleoside triphosphates are independently selected from the group consisting of deoxyadenosine triphosphate (dATP) claim 1 , deoxyguanosine triphosphate (dGTP) claim 1 , deoxycytidine triphosphate (NCTP) claim 1 , deoxythymidine triphosphate (dTTP) deoxyuridine triphosphate claim 1 , adenosine triphosphate (ATP) claim 1 , guanosine triphosphate (GTP) claim 1 , cytidine triphosphate (CTP) claim 1 , 5-methyluridine triphosphate (m5UTP) claim 1 , and uridine triphosphate (UTP).4. The nucleoside cocktail transmission medium of claim 2 , wherein said one or more nucleoside triphosphates are independently selected from the ...
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