CELLULAR COMPOSITIONS FOR TISSUE ENGINEERING

Cell compositions for tissue engineering are provided which contain a population of autologous, minimally passaged dermal fibroblasts in combination with a tissue engineering matrix or scaffold, or material forming a matrix or scaffold. In one embodiment, the population of fibroblasts is genetically engineered to secrete a therapeutic protein in an amount effective to induce tissue growth or tissue repair when the cell composition is transplanted into a subject in need thereof. For example, the therapeutic protein can be a bone morphogenic protein when the tissue to be treated is bone tissue. A preferred bone morphogenic protein is BMP-2. 1. A method of stimulating bone formation in the body , the method comprising:(a) genetically modifying autologus cells to express bone formation stimulating proteins; and(b) implanting said genetically modified autologus cells in a location in a body of a patient identified for bone formation to induce bone formation.2. A method as recited in claim 1 , wherein said genetic modification of autological cells comprises:(a) culturing a sample of cells from a patient; and(b) incubating cultured cells with an integrating or non-integrating viral vector, said vector containing genes for at least one bone stimulating protein;wherein cells are modified to express bone formation stimulating proteins by transfection of a viral vector.3. A method as recited in claim 2 , wherein said viral vector comprises an integrating lentiviral vector.4. A method as recited in claim 2 , wherein said viral vector comprises a non-integrating adenoviral vector.5. A method as recited in claim 1 , wherein said autologus cells are cells selected from the group of cells consisting of human dermal fibroblast cells claim 1 , adipose tissue cells and stem cells.6. A method as recited in claim 1 , wherein said bone formation stimulating protein is a bone morphogenic protein.7. A method as recited in claim 1 , further comprising:associating an extracellular matrix ...

NUCLEOSIDE SUPPLEMENTATION TO PROMOTE CELLULAR FUNCTION, GENETIC STABILITY AND REGENERATIVE APPLICATIONS

In various embodiments, a cell culture medium, or a nucleoside cocktail transmission (NCT) medium for the culture of stem cells with improved genetic stability, cellular function and regeneration ability is provided. Illustrative culture media comprise a basal culture medium for stem cells, where the culture medium is supplemented with one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. Illustrative NCT media comprise a delivery vehicle (e g. skin creams or other vehicle) containing one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. The NCT medium provides, inter alia, direct delivery of nucleoside cocktails into human tissues (such as skin) for regenerative, cosmetic and/or therapeutic purposes. 1. A cell culture medium for the culture of stem cells with improved genetic stability , said culture medium comprising:a basal culture medium for stem cells, where said culture medium is supplemented with one or more nucleoside triphosphates or one or more precursors thereof.2. A nucleoside cocktail transmission (NCT) medium for improving somatic or stem cell genetic stability said medium comprising:a cosmetic or pharmaceutical delivery vehicle; andone or more nucleoside triphosphates or precursors thereof.3. The cell culture medium of claim 1 , wherein said one or more nucleoside triphosphates are independently selected from the group consisting of deoxyadenosine triphosphate (dATP) claim 1 , deoxyguanosine triphosphate (dGTP) claim 1 , deoxycytidine triphosphate (NCTP) claim 1 , deoxythymidine triphosphate (dTTP) deoxyuridine triphosphate claim 1 , adenosine triphosphate (ATP) claim 1 , guanosine triphosphate (GTP) claim 1 , cytidine triphosphate (CTP) claim 1 , 5-methyluridine triphosphate (m5UTP) claim 1 , and uridine triphosphate (UTP).4. The nucleoside cocktail transmission medium of claim 2 , wherein said one or more nucleoside triphosphates are independently selected from the ...

Nucleoside supplementation to promote cellular function, genetic stability and regenerative applications

In various embodiments, a cell culture medium, or a nucleoside cocktail transmission (NCT) medium for the culture of stem cells with improved genetic stability, cellular function and regeneration ability is provided. Illustrative culture media comprise a basal culture medium for stem cells, where the culture medium is supplemented with one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. Illustrative NCT media comprise a delivery vehicle (e.g. skin creams or other vehicle) containing one or more nucleoside triphosphates (e.g., dNTPs and/or NPs) or one or more precursors thereof. The NCT medium provides, inter alia, direct delivery of nucleoside cocktails into human tissues (such as skin) for regenerative, cosmetic and/or therapeutic purposes.

Nucleoside supplementation to promote cellular function, genetic stability and regenerative applications

In various embodiments, a cell culture medium, or a nucleoside cocktail transmission (NCT) medium for the culture of stem cells with improved genetic stability, cellular function and regeneration ability is provided. Illustrative culture media comprise a basal culture medium for stem cells, where the culture medium is supplemented with one or more nucleoside triphosphates ( e . g ., dNTPs and/or NPs) or one or more precursors thereof. Illustrative NCT media comprise a delivery vehicle ( e . g . skin creams or other vehicle) containing one or more nucleoside triphosphates ( e . g ., dNTPs and/or NPs) or one or more precursors thereof. The NCT medium provides, inter alia, direct delivery of nucleoside cocktails into human tissues (such as skin) for regenerative, cosmetic and/or therapeutic purposes.

Device for preventing the locking of a vehicle wheel with a hydraulic brake system

Nucleoside supplementation to promote cellular function, genetic stability and regenerative applications

Delivery device for a powder aerosol

A delivery device (10, 110, 300, 500) for a medicament including: a housing (50), or a main body (310, 505), a receptacle (54, 600) holding a medicament in the form of a powder; and a source of propellant (53, 305), wherein the housing (50), or the main body (310, 505) provides an inlet (63, 380) and an outlet (55, 370) for the receptacle (54, 600), wherein the inlet (63, 380) is in fluid communication with the source of propellant (53, 305) and is directed against the medicament and the outlet (55, 370) is spaced from the medicament to allow aerosolization of the medicament; the device provides improved delivery efficiency, particularly a delivered fine particle fraction of greater than 20% by weight.

Delivery device for a powder aerosol

A delivery device (10, 110, 300, 500) for a medicament including: a housing (50), or a main body (310, 505), a receptacle (54, 600) holding a medicament in the form of a powder; and a source of propellant (53, 305), wherein the housing (50), or the main body (310, 505) provides an inlet (63, 380) and an outlet (55, 370) for the receptacle (54, 600), wherein the inlet (63, 380) is in fluid communication with the source of propellant (53, 305) and is directed against the medicament and the outlet (55, 370) is spaced from the medicament to allow aerosolization of the medicament; the device provides improved delivery efficiency, particularly a delivered fine particle fraction of greater than 20% by weight.