Method for detecting ecdysone content and related substances

The invention discloses a method for detecting the content of ecdysone and related substances, which adopts reversed-phase high-performance liquid chromatography, and the detection wavelength is 254nm; an ultraviolet or diode array detector is adopted; the column temperature is 25 DEG C; the flow rate is 1.1 ml/min, and the diluent is water and acetonitrile in a ratio of 50: 50 (%); v/v); the mobile phase is divided into a mobile phase A and a mobile phase B; a mobile phase A is composed of water and 0.1% trifluoroacetic acid, and a mobile phase B is composed of acetonitrile and 0.1% trifluoroacetic acid; the elution procedure is as follows: in 0-30 min, the mobile phase A is changed from 90% to 70%, and the mobile phase B is changed from 10% to 30%; 30 to 32 minutes, keeping the ratio of the mobile phase A to the mobile phase B unchanged; from 32 min to 32.1 min, the mobile phase A is changed from 70% to 20%, and the mobile phase B is changed from 30% to 80%; from 32.1 min to 35 min, the ...