Micro-nano array sensor and preparation method thereof

The invention discloses a micro-nano annular electrode sensor for quantitatively determining single cells in situ and releasing substances, and a preparation method of the micro-nano annular electrode sensor. The micro-nano annular electrode sensor comprises an annular micro-nano electrode array, a fixed-point nano modified layer, a specific election reaction modified layer and a selective cell adsorption layer, wherein the annular micro-nano electrode array is an array composed of annular micro-nano electrodes; the fixed-point nano modified layer is located on the inner surfaces of the annular micro-nano electrodes and is used for enhancing the electrode performances; the specific election reaction modified layer is formed by fixing different specific materials on the surface of the fixed-point nano modified layer and is used for correspondingly detecting different neurotransmitters of the different cells; the selective cell adsorption layer is formed on the surface of the specific election ...

Paper chip enzyme-linked immunoassay test card for combined multi-tumor marker test

The invention discloses a paper chip enzyme-linked immunoassay test card for a combined multi-tumor marker test. The test card is characterized in that upper, middle and lower filter paper chips, a double-sided tape and a paper-based slide frame are arranged between an upper shell and a lower shell; the upper, middle and lower filter paper chips refer to wax patterned filter paper chips which are provided with hydrophilic and hydrophobic regions and are alternately arranged respectively; a sample introduction and liquid feeding window is formed in an upper shell body; the upper filter paper chip is arranged between the upper shell and the double-sided tape; the middle filter paper chip is arranged between the double-sided tape and the lower filter paper chip, and sensitive regions which are distributed in arrays and used for fixing different reaction reagents are arranged on the middle filter paper chip; the water-based slide frame is arranged between the lower filter paper chip and the ...

Microelectrode array implantation type chip and manufacturing method thereof

The invention discloses a microelectrode array implantation type chip and a manufacturing method thereof. The microelectrode array implantation type chip comprises a substrate, a test electrode layer, an insulating layer and a reference electrode layer. The front end of the substrate is in a needle shape and the substrate is gradually wider from the front end to the rear end. The test electrode layer is formed on the substrate and comprises at least one microelectrode array. The insulating layer is formed on the part, apart from the area where the microelectrode array exists, of the substrate. The reference electrode layer is formed on the insulating layer, and the reference electrode layer and the microelectrode array of the test electrode layer are mutually staggered. The reference electrode layer comprises at least one reference electrode corresponding to the microelectrode array. According to the microelectrode array implantation type chip and the manufacturing method thereof, the reference ...

Non-invasive blood sugar instrument for closed-loop insulin injection

Neural microprobe for dual-mode functional positioning and precise electrical stimulation of deep brain nuclei of non-human primates

The invention discloses and provides a neural microprobe for dual-mode function positioning and precise electrical stimulation of deep brain nuclei of non-human primates. The probe is composed of a substrate layer, a metal conductive layer and an insulating layer, and the conductive layer comprises a detection site group and a stimulation site. The silicon oxide material on the back surface of the silicon substrate layer is simultaneously subjected to dry etching by adopting the double insulating layers with opposite stress, the stress difference of different materials is balanced, the technical difficulty of a lengthened silicon microprobe is overcome, and the problems that the probe is easy to bend and the mechanical stress is small due to the large length-width ratio of a silicon probe of a non-human primate animal are solved. A detection site obtains a dual-mode signal as a positioning mark by modifying a nano-metal material and a polymer anti-interference ionic membrane for functional ...

Electrode surface decoration material for biological sensors and preparation method of material

The invention relates to an electrode surface decoration material based on chitosan and a preparation method of material. More specifically, the invention provides an electrode surface decoration material which is used for biological sensors and represented by the formula (I) shown in the specification. The material is a copolymer obtained by taking chitosan as a main chain and grafting polyvinylpyridine to the main chain, wherein bpy, n, m, p and x are defined as the specification. The decoration material has excellent electrochemical characteristics, catalysis characteristics and conductive characteristics, and can be used as an electrode surface decoration material for multiple biological sensors.

Instrument device and method for quickly detecting viable count of bacillus calmette guerin vaccine

The invention discloses an instrument device for quickly detecting the viable count of bacillus calmette guerin vaccines, which relates to a biomedicine detection technique. An ultrasonic pyrolysis device for pyrolyzing the bacillus calmette guerin vaccines and a heating pyrolysis device are arranged in the instrument device so that the bacillus calmette guerin vaccines can be pyrolyzed effectively; and the instrument device is combined with an ATP bioluminescence technique and a high-accuracy weak light detection technique to realize quick detection of the viable count of the bacillus calmette guerin vaccines. A method for detecting the viable count of the bacillus calmette guerin vaccines comprises the following steps of: dissolving bacillus calmette guerin vaccine lyophiled powder, andthen putting the dissolved bacillus calmette guerin vaccine lyophiled powder into an instrument reaction tank; performing ultrasonic pyrolysis or heating pyrolysis on the dissolved bacillus calmette guerin ...

Method for rapid detection of viable bacteria content in bcg vaccine

The invention discloses a method for the rapid detection of viable bacteria content in bcg vaccine, relating to a quality inspection technology of living vaccine. The method comprises the following steps: firstly, detecting the luminous intensity of adenosine triphosphate (APT) standard solution to obtain the response relation between the APT concentration and the APT luminous intensity so as to make an equation of linear regression; utilizing buffer solution to dilute freeze-dried bcg vaccine to be a sample solution with proper concentration, carrying out ATP extraction on the sample solution; then adding bioluminescent reagent to carry out luminous detection; and finally reckoning the APT and viable bacteria content in the bcg vaccine sample according to the standard curvilinear regression equation. The method of the invention has rapid detection and simple operation, does not need culture, and is suitable for the quality inspection filed of the living vaccine and the counting of fungus ...

Wearing type non-invasive instrument for quickly testing blood sugar

A wearable non-invasive blood sugar fast tester relates to the detection technique, including a detecting instrument and a wearable component both of which are connected through a mechanical axis, wherein, the instrument can be worn on wrist, arm, ankle, etc., wherein, the detecting instrument includes an electric-acoustic-optic conversion unit, a signal preprocess unit, a central processing unit, an over-heat protection unit and a power module, these parts are in conventional electrical connection, and integrated within a shell; the electric-acoustic-optic conversion unit includes three laser diodes, a three-in-one fiber-optic and an ultrasonic sensors, three incident heads of the three-in-one optical fiber are, respectively, in coupling joint with the three laser diodes, and the emergent head is in fixed connection to the ultrasonic sensor, the three laser diodes are electrically connected to an analog switch, and the analogue switch is electrically connected to the central processing ...

Multizone multifunctional nerve dual-mode detection microelectrode array chip and preparation method

The invention discloses a multizone multifunctional nerve dual-mode detection microelectrode array chip and a preparation method thereof. The chip comprises an insulation substrate, multiple contacts, multiple strip leads and multiple microelectrode arrays. The invention also provides a preparation method of the multizone multifunctional nerve dual-mode detection microelectrode array chip. According to the technical scheme, not only the decorated electrode surface can be employed for culturing nerve cells for synchronously detecting electrophysiological signals and electrochemical signals of neurotransmitters such as acetylcholine and the like of four-group nerve cells under different experiment parameters in real time, but also the four functional zones are employed for decorating different sensitive materials and further for culturing nerve cells for detecting nerve electrophysiological signals in real time and further for comparing the characteristics of different decoration materials ...

Sanitary status on-site rapid detection device and detecting method

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the ...

Surface cleaning detection reagent for sanitation monitoring

The utility model discloses a surface cleanliness detection reagent used in health supervision, which is based on the combination of ATP biological fluorescence method and enzymatic cycling amplification technology to realize rapid detection and field assessment of health surface cleanliness. The utility model comprises ATP releaser and a fluorescent inducer with ATP regeneration enzyme; wherein, the ATP releaser can release microorganisms, somatic cells and ATP in product residues; the fluorescent inducer can react with the ATP to produce quantitative fluorescence, and then combined with the ATP regeneration enzyme to amplify the produced fluorescent signals further. The utility model, which has the advantages of convenient use of reagent, simple operation, fast response, high sensitivity and low detection cost, is widely used in health monitoring and health field supervision in food industry, cosmetic industry, medical industry and other industries.

Fluorescence quantum dot marking method used for fast bacillus coli detection

The invention provides a fluorescence quantum dot marking method used for rapid detection of escherichia coli, which relates to a detection technique and comprises the following steps: a), the Escherichia coli is marked with a fluorescence quantum dot, the fluorescence quantum dot is coupled with an Escherichia coli antibody to be taken as a probe, and then the fluorescence quantum dot-coupled antibody is combined with the pathogenic bacteria, or the Escherichia coli, in a sample to be detected; b), as the sample to be detected is exposed to exciting light, the fluorescence quantum dot marked on the surface of the Escherichia coli emits fluorescence; c), the fluorescence signal is detected by a photosensitive element; and d), the number of Escherichia coli can be obtained in a counting mode through the image identification technology, or after the total strength of fluorescence is measured, the Escherichia coli is counted through the linear relationship between the light intensity and the ...

Ultramicro planar electrode array sensor and preparation method thereof

The invention discloses a preparation method of a dual mode (two mode signals-electrophysiological signal and electrochemical signal) ultramicro planar array sensor for quantitative assay of nerve cell quantum release. An ultramicro planar electrode (0.5-5 mum) is prepared through combination of a double layer wiring design, a high precision stepping photolithography technique and a partition isolation design. According to the method, orientated nanometer modification, biological compatibility modification and specific recognition enzyme modification are combined and performed on the surface of an electrode array so as to prepare the dual mode planar ultramicro electrode array sensor. The dual mode planar ultramicro electrode array is prepared by the method combining a micro electromechanical system technique, a nanometer modification technique and a biological modification technique. Through adoption of the method, the limitations of large electrode site size (10-50 mum) of a conventional ...

Apparatus and method for fast quantitative bacteria detection

The invention provides a device and a method for rapid quantitative detection of bacteria. The method comprises the following steps: the immune technique is adopted for marking bacteria in a sample with a fluorescent substance (such as FITC, fluorescence quantum dots, and the like); as a sample solution flows through a set of filtering device, the bacteria marked with the fluorescent substance settle on the surface of a filtering membrane; the fluorescent substance on the bacteria emits fluorescence by adopting laser to induce and arouse fluorescence; and the number of the bacteria in the sample can be obtained quickly and accurately by detecting the signal strength of all the fluorescence on the filtering membrane. The entire detecting process lasts for less than 30 minutes.

Micro-nano electrode array for electrophysiological detection and electrical stimulation of spatial cognitive cells

The invention discloses a micro-nano electrode array for electrophysiological detection and electrical stimulation of spatial cognitive cells. The micro-nano electrode array comprises an electrophysiological detection site, an electrical shielding site, an electrical stimulation site, an electrophysiological wire, an electrical stimulation wire, a bonding pad site, a bonding pad wire and a substrate, the electrophysiological detection site is between the electrical stimulation site and the electrical shielding site; an electrophysiological detection site and an electric stimulation site are arranged in the electric shielding site; the bonding pad lead is connected with the electrophysiology lead and the electrical stimulation lead; the electrophysiology detection site, the electric shielding site, the electric stimulation site, the electrophysiology wire, the electric stimulation wire, the bonding pad site and the bonding pad wire are all arranged on the substrate; according to the electrode ...

Sanitary status on-site rapid detection device and detecting method

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device,the ...

Electrode probe for detecting electroencephalogram and dry electrode unit applying electrode probe

The invention provides an electrode probe for detecting an electroencephalogram and a dry electrode unit applying the electrode probe. According to the electrode probe, an anti-oxidation material such as Ag/AgCl, gold and a titanium alloy is plated on the surface of a probe head, so that the probe head can be prevented from oxidization; meanwhile, the biocompatibility of the electrode is increased; a material which has a nano effect and good biocompatibility, such as polypyrrole graphene, is modified on the surface, so that the stability and sensitivity of detection can be further improved.

Multi-site tungsten filament microelectrode for clinical brain function positioning

The invention discloses a multi-site tungsten filament microelectrode for clinical brain function positioning, a preparation method and a use method. The multi-site tungsten filament microelectrode sequentially comprises a tungsten filament, a tungsten filament insulating layer, a flexible insulating substrate layer externally connected with the tungsten filament, a metal conducting layer and an insulating layer above the conducting layer from inside to outside, wherein the metal conducting layer comprises detection sites for detecting nerve electrophysiological signals and nerve electrochemical signals, a counter electrode, a reference electrode, a wire and a bonding pad. And the detection site, the counter electrode and the reference electrode are connected with the bonding pad through wires. The obtained electrode is good in biocompatibility and not prone to deformation or fracture. The detection is stable, the size of a detection site is small, and the cell level detection can be met ...

与微机电系统工艺兼容的参比电极的制备方法

In the present invention, designed layout is formed to be mask match-board through microelectronic process and required microelectrode or electrode array can be formed by utilizing photoetching method so that electrochamical test system is finalized. The noble metal Au and Pt are used as electrode material as ultramicro-film electrode array to be used in biochamical transducer can be made with this material by applying micro-electro-mechanical system process so reference electrode which is compatible with micro-electro-mechanical system cna become a reality in practice.

Optical biosensor for detecting brain natriuretic peptide (BNP) and preparation method of reagent thereof

The invention discloses an optical biosensor for detecting brain natriuretic peptide (BNP) and a preparation method of a reagent thereof, relating to the biosensing technology. A sensor reaction zone is a capillary channel filled with magnetic nano probe solution, the inner surface of the channel is assembled with a layer of hydrophilic nano material, the upper surface of the channel is an euphotic insulating encapsulation layer with an injection hole, the side surface of the channel is encapsulated by plastics, the two ends thereof encapsulated with plastics are respectively provided with a controllable electromagnet. The preparation method of the reagent is as follows: preparing magnetic nano probes, fully reacting the magnetic nano probes, a brain natriuretic peptide (BNP) sample to bedetected and specificity BNP antigen marked by a marker under the united action of the electromagnet and the capillary channel, forming a compound of magnetic nano probes, BNP antigen to be detected and ...

Cholesterin test strip of electrochemical sensor and prepn. process of used reagent

A test bar is prepared from following steps: preparing gold film electrode on insulating material by using micro electric mechanical system (MEMS); after electrode is treated through surface activation technique, a layer of nano metal grain is assembled on reaction zone on surface of the electrode through method of electrochemical deposition; then, reaction reagent is fixed on the said layer of nano metal grain so as to form reagent layer. When blood cholesterol of object to be analyzed through capillary action adds in drop wise to reaction zone of the test bar, detection system of electrochemical biosensor detects concentration of total cholesterol rapidly.

Optical reaction tank for triphosadenine bioluminescence detection

The invention discloses an optical reaction tank used for adenosine triphosphate (ATP) bioluminescence detection, relating to the detection techniques; the optical reaction tank is composed of a cup body, a circular extension and a square lug boss; and the reaction tank is made of colorless transparent edible material with good light transmittance and is non-toxic to biological reaction. During the detection, 30uL of check sample is added to the optical reaction tank so as to realize bioluminescence intensity detection of adenosine triphosphate (ATP) through optical detection. The invention has simple design, low cost, easy use and less sample consumption, which belongs to one-time use and can be served as micro-optical reaction tanks in adenosine triphosphate (ATP) bioluminescence detection.

Neural information detection double-sided microelectrode array for three-dimensional accurate electrical stimulation induced dormancy

The invention discloses a neural information detection double-sided microelectrode array with dormancy induced by three-dimensional precise electrical stimulation. The neural information detection double-sided microelectrode array comprises double-sided electrophysiological detection sites, electrical shielding sites, electrical stimulation sites, electrophysiological wires, electrical stimulation wires, bonding pad sites, bonding pad wires and a substrate, the electrophysiological detection site is between the electrical stimulation site and the electrical shielding site; the bonding pad lead is connected with the electrophysiology lead and the electrical stimulation lead; the electrophysiology detection site, the electric shielding site, the electric stimulation site, the electrophysiology wire, the electric stimulation wire, the bonding pad site and the bonding pad wire are all arranged on the substrate; the front and back electrodes have the same arrangement mirror image; the electrode ...

Wearing type non-invasive instrument for quickly testing blood sugar

A wearable non-invasive blood sugar fast tester relates to the detection technique, including a detecting instrument and a wearable component both of which are connected through a mechanical axis, wherein, the instrument can be worn on wrist, arm, ankle, etc., wherein, the detecting instrument includes an electric-acoustic-optic conversion unit, a signal preprocess unit, a central processing unit, an over-heat protection unit and a power module, these parts are in conventional electrical connection, and integrated within a shell; the electric-acoustic-optic conversion unit includes three laser diodes, a three-in-one fiber-optic and an ultrasonic sensors, three incident heads of the three-in-one optical fiber are, respectively, in coupling joint with the three laser diodes, and the emergent head is in fixed connection to the ultrasonic sensor, the three laser diodes are electrically connected to an analog switch, and the analogue switch is electrically connected to the central processing ...

Multi-parameter immunity-chromatography test strip quantitative determination instrument

The invention relates to a multiparameter immunity chromatographic test strip quantitive detection instrument, which relates to the biochemical detection technology, adopts optical fibers as optical transmission media, simultaneously detects optical signals of a detection line and a quality control line through a CCD, analyzes the absorptivity, and obtains the concentration of an object to be detected in sample solution. The invention comprises a photoelectric detection module, a signal acquisition processing module, a control and operation module, a temperature compensation module and so on.The multiparameter immunity chromatographic test strip quantitive detection instrument adopts the method of simultaneously measuring the detection signals and the quality control signals to eliminatethe interference caused by the external environment in order to improve the stability and the sensibility of detection; the photoelectric detection module adopts the optical fibers as the transmission media ...

Surface cleaning detection reagent for sanitation monitoring

The utility model discloses a surface cleanliness detection reagent used in health supervision, which is based on the combination of ATP biological fluorescence method and enzymatic cycling amplification technology to realize rapid detection and field assessment of health surface cleanliness. The utility model comprises ATP releaser and a fluorescent inducer with ATP regeneration enzyme; wherein,the ATP releaser can release microorganisms, somatic cells and ATP in product residues; the fluorescent inducer can react with the ATP to produce quantitative fluorescence, and then combined with theATP regeneration enzyme to amplify the produced fluorescent signals further. The utility model, which has the advantages of convenient use of reagent, simple operation, fast response, high sensitivity and low detection cost, is widely used in health monitoring and health field supervision in food industry, cosmetic industry, medical industry and other industries.

Non-invasive method and device for rapidly detecting blood sugar

The invention relating to biomedicine detecting technique provides a non-invasive blood sugar fast detecting method and a device thereof. The method comprises steps of controlling a pulse laser to irradiate the skin tissue of a human body, using an ultrasonic transducer to detect the acoustic and light signals PA generated by the photoacoustic effect, using a photoelectric detector to detect scattered reflection photosignal A at the same time, and performing data analysis by using the acoustic and light signals PA and the reflection photosignal A to obtain a blood sugar concentration value to realize non-invasive blood sugar fast detecting. The device comprises a circuit part and an integrated probe, wherein, the circuit part comprises a central control processing unit and a photosignal collecting and amplifying circuit connected with the same, an acoustic and light signals collecting and amplifying circuit, a laser activation and modulation circuit, control function modules of displaying ...

Anti-interference food bacteria total number quick detection method and reagent

The utility model discloses an anti-interference fast detection method and corresponding reagent for total food bacteria amount. First, prepare the food specimen in proper sample solution according to the national standard principle; and add the non-bacteria ATP eliminator to reduce the interferences to the detection from the somatic cell ATP, the dissociated ATP and other impurities; and then carry out ATP luminous detection after orderly adding the bacteria ATP extracting agent and the bioluminescence agent; carry out the ATP standard solution luminous detection at the same time to gain the logarithm relation of the ATP density and the luminous value; at last, combine the luminous value of the food specimen to calculate the ATP density and the total bacteria amount in the sample. The method in the utility model has the advantages that the interferences of the non-bacteria ATP are completely eliminated by chemical agents without the process of bacteria increase or filtration. The method ...

Non-invasive method and device for rapidly detecting blood sugar

The invention relating to biomedicine detecting technique provides a non-invasive blood sugar fast detecting method and a device thereof. The method comprises steps of controlling a pulse laser to irradiate the skin tissue of a human body, using an ultrasonic transducer to detect the acoustic and light signals PA generated by the photoacoustic effect, using a photoelectric detector to detect scattered reflection photosignal A at the same time, and performing data analysis by using the acoustic and light signals PA and the reflection photosignal A to obtain a blood sugar concentration value torealize non-invasive blood sugar fast detecting. The device comprises a circuit part and an integrated probe, wherein, the circuit part comprises a central control processing unit and a photosignal collecting and amplifying circuit connected with the same, an acoustic and light signals collecting and amplifying circuit, a laser activation and modulation circuit, control function modules of displaying ...

Multi-parameter paper-chip electrochemical immunosensor and method for detecting lung cancer markers

The invention provides a multi-parameter paper-chip electrochemical immunosensor which comprises a covering layer, a filtering film and a foldable paperchip, wherein the covering layer is provided with a sample introduction hole, and first comb teeth which are used for exposing the end of a metal lead wire on the foldable paperchip; the filtering film is closely attached to a position below the sample introduction hole to filter and intercept large cells in a sample; the foldable paperchip comprises a first filter paperchip, a second filter paperchip and a broken line located in a middle position, sensitive areas and central drainage holes which are formed in the first filter paper chip, and working electrodes and an Ag-AgCl electrodes which are arranged on the second filter paper chip along the broken line, are symmetrically distributed; craphene-metal composite nano-materials, electronicmediators and various antibodies are decorated in the sensitive areas. After specific recognition with ...

Portable dry method instrument for quickly detecting optical and biochemical parameters

Method for detecting bacillus anthracis based on fluorescence quantum dots

The invention relates to a method for detecting bacillus anthracis based on fluorescence quantum dots. The method comprises the following steps of: firstly, specifically capturing bacteria to be detected through an antigen antibody reaction; marking fluorescent quantum dots on the bacteria to be detected to form fluorescence immune complexes by using the binding force between biotin and avidin; and detecting fluorescence signals under the action of exciting light, and realizing the quantitative detection of bacillus anthracis by establishing a standard curve. The method overcomes the defects of the traditional method and the modern method, has the advantages of high sensitivity and good specificity, is simple and convenient to operate and can complete the fast detection of anthracis based within 2 hours.

Quick bacteria cell cracking agent prescription

The invention discloses a reagent formula for fast cracking of bacterial cells, which relates to detection technical field. The reagent formula includes at least one acidic bacterial cell cracking reagent and at least one basic neutralization reagent, and at least one organic nutrient and at least one inorganic salt solvent which are added. The reagent has advantages of fast bacterial cell cracking speed, high adenosine triphosphate (ATP) output capacity with inhibition of adenosine triphosphate (ATP) digestive enzyme in the cell, convenient operation, easy preparation and low cost. The reagent formula is just suitable for fast cracking of bacterial cells in the fields of fast microbial detection.

Optical reaction tank for triphosadenine bioluminescence detection

The invention discloses an optical reaction tank used for adenosine triphosphate (ATP) bioluminescence detection, relating to the detection techniques; the optical reaction tank is composed of a cup body, a circular extension and a square lug boss; and the reaction tank is made of colorless transparent edible material with good light transmittance and is non-toxic to biological reaction. During the detection, 30uL of check sample is added to the optical reaction tank so as to realize bioluminescence intensity detection of adenosine triphosphate (ATP) through optical detection. The invention has simple design, low cost, easy use and less sample consumption, which belongs to one-time use and can be served as micro-optical reaction tanks in adenosine triphosphate (ATP) bioluminescence detection.

Polypyrrole/graphene decorated dual-mode nerve microelectrode array chip and preparation method thereof

The invention discloses a polypyrrole/graphene decorated dual-mode nerve microelectrode array chip and a preparation method thereof. The chip comprises an insulative substrate, multiple pads, multiple strip leads, four groups of microelectrode arrays; the multiple pads are distributed at the periphery of the insulative substrate at equal intervals; the four groups of the microelectrode arrays are symmetrically distributed at the center position of the insulative substrate surface; the electrodes in the microelectrode arrays respectively extends to the peripheral edge of the insulative substrate by the stripe leads connected with the pads via ends; and the surface of the work electrode in the microelectrode array is decorated with a composite sensitive film material. The invention also provides a method for preparing the chip. The chip provided by the invention helps to overcome the disadvantages that conventional nerve microelectrodes are high in impedance and an in-vitro biological tissue ...

Multi-parameter immunity-chromatography test strip quantitative determination instrument

The invention relates to a multiparameter immunity chromatographic test strip quantitive detection instrument, which relates to the biochemical detection technology, adopts optical fibers as optical transmission media, simultaneously detects optical signals of a detection line and a quality control line through a CCD, analyzes the absorptivity, and obtains the concentration of an object to be detected in sample solution. The invention comprises a photoelectric detection module, a signal acquisition processing module, a control and operation module, a temperature compensation module and so on. The multiparameter immunity chromatographic test strip quantitive detection instrument adopts the method of simultaneously measuring the detection signals and the quality control signals to eliminate the interference caused by the external environment in order to improve the stability and the sensibility of detection; the photoelectric detection module adopts the optical fibers as the transmission ...

用于电化学电极快速活化的方法

The present invention relates to quick electrochemical electrode activating method. The device or chip with the electrode to be activated is first cleaned, then fed to a plasma chamber and vacuum pumped, and finally exposure activated in oxygen and nitrogen discharging atmosphere. The present invention describes the activating process of deactivated micro electrodes, including planar micro electrode, platinum black decoration electrode and micro electrode probe. The present invention has the advantages of simple operation, fast process and high repeatability, and is suitable for activation of electrochemical electrode in device and array chip, so that the present invention may find its wide application in the research and development of biological sensor, biochip and micro system integral chip.

Flexible sensor for deep brain detection of human and non-human primates and preparation method thereof

The invention provides a flexible sensor for deep brain detection of human and non-human primates. The flexible sensor comprises a flexible electrode and a flexible printed circuit board (flexible PCB), wherein the flexible electrode comprises an implantation end and a bonding pad area, the length of the implantation end is 50-70 mm, detection sites are distributed on the implantation end, bonding pads are distributed on the bonding pad area, the detection sites are used for detecting neural signals, the bonding pads are used for being welded to the flexible PCB, and the detection sites and the bonding pads are connected through wires; the flexible electrode hierarchical structure comprises a substrate layer, a conductive layer and an insulating layer, the substrate layer and the insulating layer are poly-p-xylylene flexible films, and the conductive layer is composed of a chromium layer and a gold layer. The invention also discloses a welding method of the flexible sensor. The flexible ...

Biologic sensor for optical fast detection of surface cleanliness and its test method

The invention provides an optical biosensor used for detecting surface cleanliness rapidly, and a testing method thereof. The sensor comprises a sampling stick, a detection cavity, a liquid storage tank and a reaction chamber. The sampling stick comprises a handle, a spacing ring, a sampling swab and a pointed casing; the liquid storage tank is provided with a plurality of uniform sealing pieces which are in fixed connection through screw threads, and an ATP extractant and a neutralization reagent used for neutralizing the remaining extractant are sealed therein; and the lateral wall of the reaction chamber allows light to pass through, and a sensitive reagent is fixed at the bottom part in advance. When in detection, firstly, the sampling swab of the sampling stick is used for daubing and sampling the surface of an object to be detected; then, the pointed casing is screwed downwards; the sampling stick is inserted into the sampling detection cavity; and with the consideration of the fluorescence ...

Microelectrode array-based multichannel neural information detection system

The invention discloses a microelectrode array-based multichannel neural information detection system and relates to neurobiological detection technology. The microelectrode array-based multichannel neural information detection system comprises an analogue switch array, an electrophysiological signal detection circuit, an electrochemical signal detection circuit, an electrical stimulation signal generation circuit, multiple analogue to digital (A-to-D) converter circuits, a temperature detection circuit, a high-speed universal serial bus (USB) transmission circuit, a wireless Bluetooth communication module, data processing software to be run on a computer and the like. The multichannel neural information detection system disclosed by the invention can be used for acquiring electrophysiological and electrochemical neural information of in vivo animals or in vitro tissue; and by combining with different kinds of microelectrode arrays, the multichannel neural information detection systemcan ...

Multiple parameter micro sensor

This invention relates to sensor technique, especially to a micro-sensor integration technique. This invention is multiple-parameter sensor, which comprises carrier material, film microelectrode array, micro-reaction chamber and sampling control, wherein, the carrier material surface is located with film microelectrode array. The film microelectrode array comprises working electrode and pair electrode with leading-out ends connected with electric lead. There is micro-reaction chamber between working electrode and pair electrode, wherein, the micro-reaction chamber is connected with sampling micro-chamber. This invention in use can measure multiple biological indexes in whole blood and make the integration sensor array into disposable testing bar and its process adopts Micro Electro-Mechanical Systems (MEMES) technique, which ensures both measuring reliability and consistency and reduces production cost of testing bar.

Biologic sensor for optical fast detection of surface cleanliness and its test method

The invention provides an optical biosensor used for detecting surface cleanliness rapidly, and a testing method thereof. The sensor comprises a cylinderical sampling stick, a detection cavity, a liquid storage tank and a reaction chamber. The cylinderical sampling stick comprises a handle, a spacing ring, a sampling swab and a pointed casing; the liquid storage tank is provided with a plurality of uniform sealing pieces which are in fixed connection through screw threads, and an ATP extractant and a neutralization reagent used for neutralizing the remaining extractant are sealed therein; and the lateral wall of the reaction chamber allows light to pass through, and a sensitive reagent is fixed at the bottom part in advance. When in detection, firstly, the sampling swab of the cynlinderical sampling stick is used for daubing and sampling the surface of an object to be detected; then, the pointed casing is screwed downwards; the cynlinderical sampling stick is inserted into the sampling ...

Dual-mode flexible implantable photoelectric integrated microelectrode, preparation method and application

The invention discloses a dual-mode flexible implantable photoelectric integrated microelectrode, a preparation method and application. The flexible light source is composed of a flexible material substrate layer, a metal conducting layer, a flexible insulating layer, a flexible circuit board and a miniature optical device. The electrode is released in a laser cutting mode, and the process efficiency is improved. Photoelectric integration comprises two feasible schemes, one scheme is that an optical fiber is adhered to a flexible electrode needle handle through biological glue, and the scheme can assist in implanting a flexible electrode while integrating the optical fiber; according to the other scheme, a micro diode is packaged at the front end of the flexible electrode for direct light stimulation, and an optical fiber patch cord and an external light source are not needed. Electrophysiology and neurotransmitter dual-mode detection of electrode detection sites is realized by electrochemically ...

Method for preparing optical sensitive film for fixing human brain natriuretic peptide (BNP)

The invention discloses a method for preparing an optical sensitive film for fixing the human brain natriuretic peptide (BNP) and relates to a clinical medical diagnosis technology. The optical sensitive film can be used for detecting the BNP. The method comprises the following steps of: selecting a vector; carrying out surface treatment; fixing a captured antibody; sealing and capturing antigen; adding a detection antibody marked by a luminescent quantum dot to form a double-antibody sandwich compound, and the like, wherein the surface of the vector is treated through an aldehyde reagent, and the NBP captured antibody molecule is fixed on the surface of the vector in a mode of a covalent bond through a difunctional cross-linking agent. Because the sensitive film prepared by the method comprises the vector of which the surface is treated, the BNP captured antibody, the BNP antigen and the BNP detection antibody marked with the luminescent quantum dot, the sensitive film can be used for ...

Instrument and method for bacterium flow count testing

The invention discloses an instrument and a method for bacterium flow count testing, relating to the biomedical detection technology. The instrument is provided with built-in glass micro channels and a flow system which enables a bacterium marked by a quantum dot probe to pass individually, and can realize high-sensitivity fast detection on the specificity of the bacterium by combining the quantum dot fluorescence excitation technology and the high-precision weak-light detection technology. The method comprises the following steps: after coupling a sample to be tested to a quantum dot probe for specificity, placing the sample into a detection injection port of the instrument to enable bacteria to flow with liquid through a glass micro-channel detection area, using a laser diode to excite and induce bacteria marked with quantum dots to fluoresce, using a photomultiplier tube to detect fluorescent signals, and calculating the tested bacterial count according to the detected light intensity ...

Anti-interference food bacteria total number quick detection method and reagent

Reflection type optical-fiber bio-chemical sensing device

The invention relates to a reflection fiber biochemical sensing module that is made up of light source/probe section, light path section and test bar straining frame, which are accurately located by bolt hole and screw. Three combination modules are mutual independent that is easy to change component and maintain. Light path section adopts optical fiber. Light source, fiber and photo detector are directly coupling together. It has the advantages of small volume, high flexible, and widely application in sensor field.

Instrument device and method for quickly detecting viable count of bacillus calmette guerin vaccine

The invention discloses an instrument device for quickly detecting the viable count of bacillus calmette guerin vaccines, which relates to a biomedicine detection technique. An ultrasonic pyrolysis device for pyrolyzing the bacillus calmette guerin vaccines and a heating pyrolysis device are arranged in the instrument device so that the bacillus calmette guerin vaccines can be pyrolyzed effectively; and the instrument device is combined with an ATP bioluminescence technique and a high-accuracy weak light detection technique to realize quick detection of the viable count of the bacillus calmette guerin vaccines. A method for detecting the viable count of the bacillus calmette guerin vaccines comprises the following steps of: dissolving bacillus calmette guerin vaccine lyophiled powder, and then putting the dissolved bacillus calmette guerin vaccine lyophiled powder into an instrument reaction tank; performing ultrasonic pyrolysis or heating pyrolysis on the dissolved bacillus calmette guerin ...

Automatic control device suitable for electrochemistry parameter monitoring experiment

The invention discloses an automatic control device suitable for an electrochemistry parameter monitoring experiment, relating to an automatic device technology. The device comprises a computer, a microcontroller module, a relay array, a communication interface, a multi-pump system and an additional module, wherein the communication interface is used for controlling the coupling of software and hardware of the device; the multi-pump system is used for controlling the automatic sample injection of a reagent according to preset experiment flows; the additional module is used for warning in the experiment; and control console software based on Windows is solidified in the hard disk of the computer. The control device can control the whole electrochemistry working station system to work according to a preset experiment flow in a mode of combining software and hardware and control the relay array to carry out electrode switching and realize automatic sample injection. The control device has ...

Reagent formula for fastly inspecting glutamic-pyruvic transaminase by optical method

Kit for quantitatively detecting CEA (Carcino-Embryonic Antigen) as well as preparation method and application method of kit

The invention discloses a kit for quantitatively detecting a CEA (Carcino-Embryonic Antigen). The kit comprises following reagents: a biological functional magnetic nano composite particle coupled with a CEA antibody, an enzyme-labeled CEA antibody and a chemiluminiscence substrate, wherein the biological functional magnetic nano composite particle coupled with the CEA antibody is formed by compounding a nano-grade magnetic nucleus with a high molecular material containing various functional groups. According to the kit for quantitatively detecting the CEA, a nano magnetic bead separation technology, a chemiluminiscence technology and an enzyme immunoassay technology are combined together; the kit has the advantages of high sensitivity, wide linearity range, high specificity, rapidness in reaction and the like.

Fast detector for ultra-trace disease markers

The invention discloses a fast detector for ultra-trace disease markers, relating to a detection technology. The fast detector comprises a 37 DEG C constant-temperature heating system, a flow injection system and a detection control system, wherein in the 37 DEG C constant-temperature heating system, a temperature sensor detects a heating cup closely pressed by semiconductor heating sheets at real time, and continuously adjusts the semiconductor heating sheets to achieve the 37 DEG C constant-temperature heating function, thereby providing a 37 DEG C incubation environment for the biological reaction; a peristaltic pump is used for realizing flow injection and finishing automatic sampling so as to reduce human error and shorten detection time; the detection control system comprises a photoelectric conversion unit, a photon counting unit and a central processing and control unit; and a high-sensitive photomultiplier tube is used for realizing weak light detection, and the detection cavity ...

Reagent preparing method for rapidly detecting blood ketone body

The invention discloses a reagent preparation technique which can fast test the blood ketone. The technique includes first assemble a level of metal nanometer particles on pre-processed electric pole with electrochemical deposit technique. And then fix the reaction reagent on the nanometer particle layer forming reaction layer. When blood ketone drop is dropt on the reaction layer, the basal material will reaction with the solution generate electric signals testable with electrochemistry bio sensor testing system outputting corresponding intensity signal.

一种用于制备毛细管沟道及其取样的方法

A technology for preparing the capillary channel and sampling it by use of novel polydimethyl siloxane (PDMS) and negative photoetching epoxy material (SU8) includes such steps as designing the capillary channel by software, preparing mask, coating SU8 on substrate, photoetching on SU8 to obtain open microchannel or microchannel model, using copy technique of PDMS to consitute flow way of capillary channel, and dripping the sample solution to one end of said channel until it is full of the sample solution. Its advantages are simple process, high repeatability and low cost.