23-10-2014 дата публикации
Номер: US20140311199A1
Принадлежит:
The invention relates to a method for multiplying a strain of , which includes the preparation of substantially contaminant-free culture media, containing disinfected wood fibers obtained by means of a twin-screw extruder, the amplification of via a series of steps for manufacturing a primary inoculum and then a secondary inoculum from said primary inoculum, and the multiplication of the microorganisms in each step, wherein the multiplication of the propagules reaches 2×10to 10. 1Trichoderma. A method for multiplying a strain of , which comprises:{'i': 'Trichoderma', 'sup': '2', 'the inoculating, with a stock suspension of propagules, of a first sterilized culture medium containing between approximately 25% and approximately 50% by weight of wood fibers, water, nutritive substances and a pH modifier, said fibers having an air content by volume included in the range of from approximately 55% to approximately 90%, said medium being substantially free of fungal contaminants and having a bacterial contaminant content of less than or equal to 10CFU/g;'}{'sup': 4', '5, 'the culturing of the culture medium thus inoculated so as to obtain a primary inoculum in which the multiplication of the propagules reaches 2×10to 10.'}2. The multiplication method as claimed in claim 1 , in which the final concentration of propagules in the primary inoculum is in the range of from 10to 10propagules/g.3. The method as claimed in claim 1 , in which the culturing of the medium comprises an incubation period of at least two weeks.4. The method as claimed in claim 1 , which also comprises:the preparation of a second culture medium containing wood fibers which have been disinfected, said wood fibers having an air content by volume included in the range of from approximately 55% to approximately 90%;the inoculation of said second culture medium with 0.001% to 5% by weight of the primary inoculum;{'sup': 3', '5, 'the culturing of the culture medium thus inoculated so as to obtain a secondary ...
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