Lilium regale cytochrome b5 gene LrCyt-b5 and application thereof

The present invention relates to a Lilium regale cytochrome b5 gene LrCyt-b5 with antifungal activity and application thereof. The LrCyt-b5 gene has nucleotide sequence shown as SEQIDNO:1 and encodes cytochrome b5. Through research on functional genomics related technology, the invention has confirmed that LrCyt-b5 gene has the function of improving fungal infection resistance of plants. The antifungal LrCyt-b5 gene provided by the invention can be constructed to a plant expression vector and transferred to tobacco for over expression, so that the transgenic tobacco plants gain strong in vitro antifungal activity. Transgenic tobacco with over expression of LrCyt-b5 has obvious inhibition effect on growth of Fusarium oxysporum, Botrytis cinerea, Rhizoctonia solani and Botryosphaeria.

Method for extracting total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites

The invention provides an extration method of plant RNA by utilizing guanidinium isothiocyanate-chloroform extractive and Tris purification. The invention improves traditional guanidinium isothiocyanate method, adopts the two steps of chloroform extration after cracking and phenol-adding for purification, and respectively extract high-quality total RNA from the leaf blade and seed case of red-skinned pears, the leaf blade, fruit flesh and seed case tissues of grapes, the fruit of strawberries and the callus of arnebia euchroma. The extraction buffer liquid in the method contains guanidinium isothiocyanate, sodium dodecyl sarcosinate, sodium citrate, soluble polyvinylpyrrolidone and beta-mercaptoethanol, which can not only crack plant cells efficiently and release RNA, but also effectivelyinhibit the activity of RNA enzyme, and can also prevent the oxidation of phenolic compounds and suppress the interference of secondary metabolites. In the second step, Tris purification is utilized for ...

Application of lilium regale germin protein gene LrGLP1

The invention relates to an application of a lilium regale germin protein gene LrGLP1. The nucleotide sequence of the LrGLP1gene is shown as SEQ ID NO:1, a germin-like protein is encoded, and according to the invention, functional genomics related technical researches prove that the LrGLP1gene has a function of improving the fungal infection resistance of plants. An antifungal LrGLP1 gene in the invention is built into a plant expression vector, and transferred into a tobacco to be subjected to excessive expression, so that a transgenic tobacco plant has an extremely strong in-vitro antifungal activity. LrGLP1overexpressed transgenic tobaccos have an obvious inhibitory effect on the growth of alternaria alternate, sclerotinia sclerotiorum and catenuliform gibberella.

High concentration drip irrigation fertilizer exclusively used in fruit enlargement period of grapes and preparation method thereof

The invention provides high concentration drip irrigation fertilizer exclusively used in the fruit enlargement period of grapes utilized in a drip irrigation system and a preparation method thereof. The main components of the drip irrigation fertilizer exclusively used in the fruit enlargement period of grapes are as follows (according to the parts by weight): 611-723 parts of potassium nitrate, 90-108 parts of monoammonium phosphate, 33-41 parts of urea, 124-256 parts of magnesium sulfate heptahydrate, 0.3-0.6 part of boric acid, 3.6-5.8 parts of EDTA chelated iron, 0.2-0.4 part of EDTA chelated copper, 0.6-1.0 part of chelated manganese, 0.002-0.004 part of sodium molybdate and 1.0-1.6 parts of EDTA chelated zinc. The drip irrigation exclusively used fertilizer has dramatic effects, canobviously improve the yield and quality of grapes, and can save fertilizer and improve efficiency. The drip irrigation exclusively used fertilizer combines the domestic all-pervading trend of the irrigation ...

Application of lilium regale wilson pathogenesis-related protein 10 gene LrPR10-5

The invention discloses an application of a lilium regale wilson pathogenesis-related protein 10 gene LrPR10-5. A nucleotide sequence of the LrPR10-5 is as shown in SEQIDNO:1, and the pathogenesis-related protein 10 gene is encoded. The research proves that the LrPR10-5 gene has the function of improving the plant antifungal capability by functional genomics-related technologies, the antifungal LrPR10-5 gene is built on a plant expression vector and is switched into tobacco to perform overexpression, the transgenic tobacco plant has strong in-vitro antifungal activity in the result, and the experiment result shows that the transgenic tobacco for overexpression of the LrPR10-5 has an obvious inhibiting effect on growth of a plurality of fungi, such as botrytis cinerea, rhizoctonia solani and sclerotinia sclerotiorum.

Walnut oil fat emulsion and manufacturing method thereof

High concentration drip irrigation special fertilizer for vegetative growth phase of grapes

The invention provides a high concentration drip irrigation special fertilizer for the vegetative growth phase of grapes. The fertilizer comprises the following main components in part by weight: 244 to 267 parts of potassium nitrate, 181 to 198 parts of monoammonium phosphate, 357 to 390 parts of urea, 139 to 209 parts of magnesium sulfate heptahydrate, 0.3 to 0.6 part of boric acid, 3.6 to 5.8 parts of EDTA chelated iron, 0.2 to 0.4 part of EDTA chelated copper, 0.6 to 1.0 part of EDTA chelated manganese, 0.002 to 0.004 part of sodium molybdate, and 1.0 to 1.6 parts of EDTA chelated zinc. The special fertilizer is especially designed for a drip irrigation system, and is suitable for the requirement on each element at the vegetative growth phase of the grapes. The special fertilizer can obviously improve the photosynthesis of plants and the nutrient substance accumulation amount of leaves, establishes a firm substance foundation for quick inflation of fruits and fruit growth in later period ...

Cactus Juice and its making method

Glutathione S-transferase gene LrGSTL1 of lilium regale and application thereof

The invention discloses an anti-disease gene (LrGSTL1) of lilium regale and an application thereof. The nucleotide sequence of the gene (LrGSTL1) is shown as SEQIDNO:1 to encode Lambda glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTL1) of lilium regale has the function of improving the antifungal ability of the plant. The glutathione S-transferase gene (LrGSTL1) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed. The transgenetic tobacco has strong antifungal activity, and the transgenetic tobacco sheet which expresses (LrGSTL1) has remarkable resistance to infection by fusarium oxysporum and botrytis cinerea.

Application of Yangbi big-bubble walnut transcription factor gene JsWRKY1

The invention discloses application of a Yangbi big-bubble walnut transcription factor gene JsWRKY1. The JsWRKY1 gene is proved to have a function of enhancing the fungal disease resistance of a plant through technical research related to functional genomics. The antifungal gene JsWRKY1 is established to a plant expression vector and transferred into a tobacco for overexpression, and a transgenic tobacco plant has very high in-vitro antifungal activity, and a transgenic tobacco subjected to JsWRKY1 overexpression achieves obvious inhibiting effect on the growth of botryosphaeria dothidea, moniliform gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

Walnut condensed milk and its prodn process

Black tea extract rich in statins and preparation technique and use thereof

The invention relates to a black tea extract which is rich in statins compounds and the preparation technology and usages thereof. The black tea extract comprises the following main chemical constituents: 10-80 percent of the statins compounds and the balance tea polyphenol, theophyline and tea pigment in terms of dry basis or dry matter content; the physical form can be slime, paste, granula, powder and block. The preparing techniques include the following steps: the black tea is ground, the solution is ultrasonically extracted and centrifugalized, thus the extracted solution and tea residueare obtained; the extracted solution can be obtained by concentration and dryness. The black tea extract preparation technology provide a novel way for the tea deep processing and has the advantages of high added value, extensive application of products, easy obtaining of the crude materials, easy and rapid technology and high production efficiency, etc.

Puer tea breakfast nutritious powder

The invention relates to a puer tea breakfast nutritious powder with the function of lowering blood fat, belonging to the field of food science technique. The powder is characterized in that puer tea extraction is added into breakfast nutritious powder. The breakfast nutritious powder is formulated by 0-50 percent of puffed cereals powder, 0-20 percent of puffed legume powder, 0-15 percent of fruit and vegetable (pumpkin, carrot, yam, date and barbary wolfberry fruit) powder, 0-15 percent of nutlet (black sesame, walnut seed, peanut and sunflower seed) powder and 0.1-10 percent of puer tea extraction. The puer tea extraction is rich in caffeine, lovastatin, tea polyphenol, thea-flavin, thea-browin and tea polysaccharide, which are functional compositions of exciting the central nervous system and lowering blood fat. Thereby, the puer tea breakfast nutritious powder prepared by the invention not only has high nutritious value, but also has good functions in lowering blood fat and prevention ...

Method for finishing financial data-handling with video terminal and public payment server

The invention discloses a kind of method to process finance data by visible terminal, said visible terminal connects with the public pay system by condition acceptance subsystem, and said condition acceptance subsystem is used to display the programs which accords with the condition on visual terminal, said condition acceptance subsystem has an intelligent card, said public pay systems stores the corresponding relationship between the unique identification information with one or several finance accounts, the method includes: the condition acceptance subsystem sends the unique identification information and the relative trading data to the public pay system; and the public pay system sends the relative trading data and the information of finance account to the corresponding processing terminal of finance data according to the corresponding relationship; and the processing terminal of finance data returns the processing result to the public pay system; and the public pay system returns the ...

Method for reinforcing alkannin synthesis in Onosma paniculatum cell cultivation process

The invention provides a method for strengthening synthesis of alkannin in the process of culturing an onosma paniculatum Bur.et Franch cell. The method mainly comprises the following operation steps of: 1, succeeding transfer culture of the onosma paniculatum Bur.et Franch cell; 2, promotion of the synthesis of the alkannin of the onosma paniculatum Bur.et Franch cell by chitosan; and 3, the process of measuring the content of the alkannin. The content of the alkannin of the onosma paniculatum Bur.et Franch cell can be improved by adding the chitosan to a culture medium. The method has the advantages of easy operation and good effect, and can be applied to actual production of useful secondary metabolic products by mass culture of vegetable cells.

Security information interaction system, device and method

The invention provides a security information interaction system, a device and a method. The security information interaction system comprises a security information interaction terminal, an access server and a security information interaction device, wherein the security information interaction terminal is used for receiving a security information interaction request of a user and transmitting the security information interaction request to the access server. The security information interaction request comprises information interaction data, an identifier correlative with the security information interaction terminal and an identifier correlative with the related security information interaction device. The security information interaction system, the device and the method are high in security and low in cost.

Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis

The invention provides a method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis, comprising the following steps of: grinding walnut raw materials into walnut powder, adding 100,000 to 1000,000 units of protease or composite protease to 1kg of walnut powder, adding water in the mass ratio of the walnut powder to the water is 1: (0.5-20), and then performing microwave processing for 1-10 minutes under the stirring condition of 60 to 300rpm when the microwave power is 10 to 1000 W/L and the temperature is 35 to 65 DEG C, and performing centrifugal separation of oil phase, aqueous phase and solid phase after the reaction ends, wherein the oil phase is walnut oil, the aqueous phase is walnut protein peptide, and the solid phase is residues. The method provided by the invention has mild extraction condition and excellent product performance, and can improve the efficiency of ingress of oil. Furthermore, the obtained walnut oil is higher ...

Method for promoting synthesis of Onosma paniculatum cell alkannin by using rare-earth element

The invention relates to a method for promoting the synthesis of the shikonin of an onosma paniculatum cell by using rare earth elements, which mainly includes the following operation steps of: 1. the successive transfer culture of the onosma paniculatum cell; 2. the promotion on the synthesis of the shikonin of the onosma paniculatum cell by the rare earth elements; and 3. the detecting process of the content of the onosma paniculatum. Adding the rare earth elements in a culture medium can improve the content of the shikonin of the onosma paniculatum cell. The method is simple to be operated and has excellent effect. The method can be applied to the actual production process of culturing and producing useful secondary metabolites by plant cells in large scale.

Fresh gastrodia tuber full powder and preparation technique thereof

The invention relates to a Castrodia elata BL powder and a preparation process thereof, belonging to the deep processing technology of the Chinese medicine Castrodia elata BL. The Castrodia elata BL powder is prepared by the following steps: Castrodia elata BL is washed, and pulped or ground into mud or pulp under the condition of restraining or killing enzyme, and finally the Castrodia elata BL mud or pulp is frozen and dried. The Castrodia elata BL powder obtained by the process maintains all nutrition and efficacy ingredients of Castrodia elata BL except water.

Juglans sigillata Dode chitinase gene JsCHI1 and application thereof

The invention discloses a Juglans sigillata Dode chitinase gene JsCHI1 and application thereof. The nucleotide sequence of the JsCHI1 gene is disclosed as SEQ ID NO:1. According to the coding chitinase, the research by functional genomics related technology confirms that the JsCHI1 gene has the function of enhancing fungus infection resistance of the plant. After the antifungal JsCHI1 gene is constructed into a plant expression vector and transformed into tobacco for overexpression, the transgenic tobacco plant has very high in-vitro antifungal activity. The JsCHI1-overexpressed transgenic tobacco has obvious inhibiting actions on growth of Fusarium solani, Fusarium oxysporum, Botryosphaeria dothidea and Gibberella moniliformis.

Pumpkin enzyme health wine and preparation method thereof

The invention discloses pumpkin enzyme health wine and a preparation method thereof. The pumpkin enzyme health wine comprises raw materials in parts by weight as follows: 12-20 parts of pumpkin enzymes, 5-22 parts of wine and 0.1-0.4 parts of a flos puerariae extract. The pumpkin enzyme health wine contains a large quantity of effective ingredients of the pumpkin enzymes, has aroma of baijiu, is rich in nutrition, tastes better, contains a large number of pumpkin polysaccharides, carotenoid, amino acid and mineral substances, can promote bone development and metabolism of human bodies, resist tumor, clear away heat and remove toxins and has various adjusting functions on the immune system; by adding a proper quantity of isoflavone compounds alcohol absorbed by gastrointestinal tracts is effectively reduced, the activity of ethanol dehydrogenase is promoted, the purpose of protecting the gastrointestinal tracts damaged due to long-time or heavy drinking is achieved, the health wine has very ...

ELISA (enzyme linked immunosorbent assay kit) for qualitatively and quantitatively detecting walhut albumen and detection method thereof

The invention discloses an ELISA (Enzyme Linked Immuno sorbent Assay) kit for qualitatively and quantitatively detecting walhut albumen and a detection method thereof. The kit comprises an ELISA plate coated with primary antibody, a positive antigen walhut albumen, an ELISA secondary antibody, a diluent, a substrate development solution, a cessation solution and a washing solution. The kit is characterized in that antibodies fixed on a microporous surface of the ELISA plate are used for capturing the walhut albumen in a standard solution or a sample solution, the ELISA plate antibody identifies the walhut albumen and is combined with the walhut albumen to form an antibody-walhut albumen-antibody-enzyme compound; after the enzyme and a substrate react and have color development, the absorbance is detected; the concentration of the walhut albumen in a sample is quantitatively calculated according to a standard curve, and further the walhut albumen is subjected to quantitative detection and ...

Preparation and application of walnut shell flavone lipid-decreasing active ingredient

The invention discloses a preparation and an application of a walnut shell flavone lipid-decreasing active ingredient, belonging to the field of food medicines. The preparation is characterized in that the lipid-decreasing active ingredient 5-hydroxy-4'-methoxy-7-methyl flavone is prepared by performing alcohol extraction on walnut shells, extracting by using an organic solvent and separating and purifying by using a silica gel column. The comprehensive utilization values of walnut shells are increased.

Special foliar fertilizer for pericarp color formation of bagged grape and preparation method thereof

The invention discloses a formula for solid instant special foliar fertilizer which can promote the color formation of bagged grape fruit and a production method thereof, belonging to the field of fertilizer. The special foliar fertilizer mainly comprises the following components according to weight percentage: 2.1-6.1 % of glucose, 6-10% of nitrogen, 16-20% of phosphorus, 26-30% of potassium, 0.5-1.3% of magnesium, 1.2-2.9% of sulphur, 0.1-0.3% of ferrum, 0.015-0.04% of copper, 0.03-0.1% of manganese, 0.015-0.04% of zinc, 0.006-0.018% of boron and 0.0015-0.004% of molybdenum. The special foliar fertilizer has obvious effect, can obviously improve synthesis and transportation of enocianina and improves grape sugar content and acre yield, the contained elements and substances are beneficial to plants, so that the special foliar fertilizer has wider market and developing and applying value. The method for preparing the special foliar fertilizer can avoid fertilizer agglomeration, and ensures ...

Method for regenerating Onosma paniculatum plants

The invention relates to a method for regenerating Onosma paniculatum plants through calluses, comprising the following steps of: (1) inducing embryonic calluses of Onosma paniculatum; (2) carrying out successive transfer culture on the embryonic calluses; (3) differentiating cluster buds of the embryonic calluses; (4) strengthening the cluster buds and inducing roots; and (5) hardening seedlings and transplanting. The embryonic calluses with high propagation coefficient can be obtained with the method, the obtained Onosma paniculatum regeneration plants have high survival rate and good growth state, and the method can be applied to the actual production process of cultivating the artificial Onosma paniculatum plants on a large scale.

Dustproof quick -witted case of computer that radiating effect is good

The utility model discloses a dustproof quick -witted case of computer that radiating effect is good, including case body, the heat dissipation fan being installed in case body left side, and heat dissipation fan externally mounted has a dust screen, the case body front portion is provided with switch, pilot lamp, USB interface and optical drive, the case body rear portion is equipped with the vent, and the case body rear portion is provided with wire interface, case body internally mounted has temperature sensor and fan. The utility model discloses a setting up vent and heat dissipation fan, making its radiating effect good, and at heat dissipation fan externally mounted dust screen, can prevent that the dust from getting into quick -witted case, through setting up temperature sensor, when the dust gathering of dust screen was too much, temperature sensor detected quick -witted case high temperature, and the dust on the dust screen was blown off in fan work this moment, made quick -witted ...

Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide

The invention relates to an enzyme method ultrasonic extraction method of walnut oil and walnut protein, which belongs to the food and the functional food field. Water is added into walnut kernel or walnut powder to be grinded into paste, protease or compound protease are added into to be performed hydrolization, and simultaneously ultrasonic processing is performed, then the walnut kernel or walnut powder is agitated to perform enzymatic extraction, and then centrifugal separation oil phase, protein peptide oil water phase and residual solid phase are performed; walnut oil is acquired by refining the obtained oil phase, which meets the requirements of green foods; the protein peptide aqueous solution can be directly used to produce degreased walnut protein nutrient milk, or to prepare walnut antioxidation peptide after performed nanofiltration, which is used in health products, food additives, cosmetics or daily chemical articles, or to produce nutrient condensed milk after being performed ...

Cell phone terminal onsite paying method and system based on mobile positioning service

A cell phone terminal onsite paying method and system based on mobile positioning service is provided; the method and system connects a cell phone terminal number with payment card information of a cell phone terminal user in advance, and registers the terminal payment equipment users to a server-side based on the mobile positioning service in advance; according to the cell phone position information obtained by the mobile positioning service, a terminal payment equipment user close to the cell phone terminal is selected from the registered terminal payment equipment users, and the select result is sent to the cell phone terminal so as to choose a transaction user; the server-side sends an authorization code to the cell phone terminal after the transaction user and transaction set information are received; the terminal payment equipment sends the authorization code and transaction related information to the server-side, and sends the transaction related information and payment card information ...

System and method of coupon payment

The invention discloses a system and a method of coupon payment. The system comprises a coupon management module, a non-contact communicating module, a coupon calculation module and a financial account module, wherein the coupon management module stores coupon data. The non-contact communicating module is configured as follows: reading the coupon data from the coupon management module; receiving original payment amount from the exterior; sending the read coupon data and the original payment amount to the coupon calculation module; acquiring used coupon data and final payment amount from the coupon calculation module; communicating with the financial account module to perform payment treatment according to the final payment amount; communicating with the coupon management module according to the used coupon, so that the coupon management module cannot reuse the used coupon. The coupon calculation module is configured as follows: determining the used coupon according to the coupon data and ...

Glutathione S-transferase gene LrGSTU3 of lilium regale and application thereof

The invention discloses a gene (LrGSTU3) of lilium regale with antifungal activity and an application thereof. The nucleotide sequence of the gene (LrGSTU3) is shown as SEQIDNO:1 to encode tau glutathione S-transferase. Functional genomics related technical research shows that the gene (LrGSTU3) of lilium regale has the function of improving the antifungal ability of the plant. The gene (LrGSTU3) of lilium regale disclosed by the invention is constructed to a plant expression vector and transformed to tobacco to be over-expressed, so that the transgenetic tobacco strain is strong in vitro antifungal activity, and the transgenetic tobacco sheet which over-expresses (LrGSTU3) has a remarkable inhibiting effect on growth of fusarium oxysporum and botrytis cinerea.

Method of preparing novel Puer tea paste

The invention relates to a novel Puer Tea ointment and the production method thereof, which belongs to a technical field of food and drug. The material is Puer Tea, while the basic process of production is as follows: warm water ultrasonic extracting the crushed Puer Tea and pressing, concentrating under low-temperature and vacuum-evaporating to acquire the low temperature extracted Puer Tea ointment, then low temperature extracting, pressing and concentrating the tea residue and tea cake that is acquired through the high temperature extraction to acquire the high temperature extracted Puer Tea ointment, mixing the two ointments to press the novel Puer Tea ointment with each component completely reserved. The invention introduces in techniques of low temperature ultrasonic extraction and pressing, and overcomes the defect that high temperature boiling water extraction causes damage to the original active flora and active components, thus reserving the special biological activity of the ...

Method for extracting total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites

The invention provides an extration method of plant RNA by utilizing guanidinium isothiocyanate-chloroform extractive and Tris purification. The invention improves traditional guanidinium isothiocyanate method, adopts the two steps of chloroform extration after cracking and phenol-adding for purification, and respectively extract high-quality total RNA from the leaf blade and seed case of red-skinned pears, the leaf blade, fruit flesh and seed case tissues of grapes, the fruit of strawberries and the callus of arnebia euchroma. The extraction buffer liquid in the method contains guanidinium isothiocyanate, sodium dodecyl sarcosinate, sodium citrate, soluble polyvinylpyrrolidone and beta-mercaptoethanol, which can not only crack plant cells efficiently and release RNA, but also effectivelyinhibit the activity of RNA enzyme, and can also prevent the oxidation of phenolic compounds and suppress the interference of secondary metabolites. In the second step, Tris purification is utilized for ...

Application of Lilium regale pathogenesis-related protein 10 gene LrPR10-6

The present invention discloses application of a Lilium regale pathogenesis-related protein 10 gene LrPR10-6. LrPR10-6 has nucleotide sequence shown as SEQ ID NO:1 and encodes pathogenesis-related protein 10. Through research on functional genomics related technology, the invention has confirmed that LrPR10-6 gene has the function of improving fungi resistance of plants. The anti-fungal LrPR10-6 gene provided by the invention can be constructed into a plant expression vector and transferred to tobacco for over expression, so thaytthe transgenic tobacco plants gain strong in vitro antifungal activity. The experimental results show that the transgenic tobacco with overexpression of LrPR10-6 has significant inhibitory effect on Botrytis cinerea, Sclerotinia sclerotiorum and Fusarium oxysporum.

Method for improving content of phenethyl alcohol glucoside in cistanche salsa cultured cell

The invention relates to a method for improving the content of phenethyl alcohol glucoside in cistanche salsa cultured cells, which mainly comprises the following operation steps: carrying out the successive transfer culture of cistanche salsa cells; 2. carrying out the liquid culture and ultrasonic treatment of the cistanche salsa cells; and 3. measuring the content of the phenethyl alcohol glucoside. Using ultrasonic waves to stimulate the cistanche salsa cells in suspension culture is an effective means for improving the content of phenethyl alcohol glucoside in the cells. The method has simple operation, good effect and strong expansibility, can be applied to the mass culture process of a cistanche salsa cell bioreactor, and can improve the content of medicinal secondary metabolites of the cultured cells.

Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide

The invention relates to an enzyme method ultrasonic extraction method of walnut oil and walnut protein, which belongs to the food and the functional food field. Water is added into walnut kernel or walnut powder to be grinded into paste, protease or compound protease are added into to be performed hydrolization, and simultaneously ultrasonic processing is performed, then the walnut kernel or walnut powder is agitated to perform enzymatic extraction, and then centrifugal separation oil phase, protein peptide oil water phase and residual solid phase are performed; walnut oil is acquired by refining the obtained oil phase, which meets the requirements of green foods; the protein peptide aqueous solution can be directly used to produce degreased walnut protein nutrient milk, or to prepare walnut antioxidation peptide after performed nanofiltration, which is used in health products, food additives, cosmetics or daily chemical articles, or to produce nutrient condensed milk after being performed ...

Malania oleifera lectin and method for preparing same

The invention provides malania oleifera lectin and a method for preparing the same. The method comprises the following steps: grinding malania oleifera, stirring and socking the ground malania oleifera into cold water, grading and precipitating by using ammonium sulfate, ultrafiltering to remove salt, concentrating, carrying out ion exchange chromatography, purifying, ultrafiltering to remove salt, concentrating, freezing and drying to obtain the refined malania oleifera lectin. The prepared malania oleifera lectin has the molecular weight of about 60kd, the electrophoresis purity of above 90% and the potency of 1:10<5> or more, namely the HIV infected cell (inhibition concentration 50) IC50 of 1:10<5>, can agglutinate the red blood cells of a rabbit, a pig, a chicken, a duck and a mouse and can be used for preventing HIV infection.

Ultra low temperature freezing-storage method for onosma paniculatum cell

The invention relates to a super cryopreservation method for onosma paniculatum cell. The method mainly comprises the operating steps: firstly, pretreatment and dehydration process before the cryopreservation of the cell; secondly, cryopreservation stage of the cell in liquid nitrogen; thirdly; thawing process of the cryopreservation cell; fourthly, resuscitation and cultivation of the cell after the thawing. When the onosma paniculatum cell is preserved by utilizing the super cryopreservation method, the cell can obtain higher survival rate after being thawed, and the method is an effective way of preserving the onosma paniculatum germplasm resource.

Method for regenerating Onosma paniculatum plants

The invention relates to a method for regenerating Onosma paniculatum plants through calluses, comprising the following steps of: (1) inducing embryonic calluses of Onosma paniculatum; (2) carrying out successive transfer culture on the embryonic calluses; (3) differentiating cluster buds of the embryonic calluses; (4) strengthening the cluster buds and inducing roots; and (5) hardening seedlingsand transplanting. The embryonic calluses with high propagation coefficient can be obtained with the method, the obtained Onosma paniculatum regeneration plants have high survival rate and good growth state, and the method can be applied to the actual production process of cultivating the artificial Onosma paniculatum plants on a large scale.

Preparation and application of sage polysaccharides and its esters

The invention relates to the preparation and application of Salvia polysaccharide and its esters. The sugar chain is sorbitol, rhamnose, and galactose linked through 1->6 (or non-reducing terminal), 1->4 and 1->3 glycosidic bond. The invention provided Salvia polysaccharide and its esters is prepared from Salvia as raw material or residues of Salvia after extracting fat-soluble effective ingredient or (and) water soluble phenolic acid by leaching with hot water, concentrating, precipitating with ethanol, deproteinizing to obtain Salvia polysaccharide, and esterizing to obtain Salvia polysaccharide ester (sulfate, phosphate ester, nitric ether and acetic ester).The invention obtained Salvia polysaccharide and its esters both have strong human immunodeficiency virus and herpes simplex virus resisting activity, has low toxicity to human cell, and can be used for preparing preparation for preventing or treating AIDS viral and herpes simplex viral infection.

Method for increasing alkannin content of onosma paniculatum cultivation cell

The invention relates to a method for increasing the alkannin content of the cultured cells of onosma paniculatum. The operating steps of the method mainly comprise: 1. the subculture of onosma paniculatum cells; 2. the process of liquid culture and ultra sonication of the onosma paniculatum cells; and 3. the process of measuring alkannin content. Utilizing ultrasonic to stimulate the suspension cultured onosma paniculatum cells is an effective means to increase the alkannin content of cells. The method is characterized by simple and easy operation, good effect and strong expansibility and can be applied to a large-scale bioreactor for plant cells to improve the content of the effective secondary metabolites.

Ultra low temperature freezing-storage method for onosma paniculatum cell

The invention relates to a super cryopreservation method for onosma paniculatum cells. The method mainly comprises the operating steps: firstly, pretreatment and dehydration process before the cryopreservation of the cells; secondly, cryopreservation stage of the cells in liquid nitrogen; thirdly; thawing process of the cryopreservation cells; and fourthly, resuscitation and culturing of the cells after the thawing. When the onosma paniculatum cells are preserved by utilizing the super cryopreservation method, the cells can obtain higher survival rate after being thawed, and the method is an effective way for preserving the onosma paniculatum germplasm resource.

Juglans sigillata Dode ribonuclease gene JsRNase and application thereof

The invention discloses a Juglans sigillata Dode ribonuclease gene JsRNase and application thereof. The nucleotide sequence of the JsRNase gene is disclosed as SEQ ID NO:1. According to the coding ribonuclease, the research by functional genomics related technology confirms that the JsRNase gene has the function of enhancing fungus infection resistance of the plant. After the antifungal JsRNase gene is constructed into a plant expression vector and transformed into tobacco for overexpression, the transgenic tobacco plant has very high in-vitro antifungal activity. The JsRNase-overexpressed transgenic tobacco has obvious inhibiting actions on growth of Colletotrichum gloeosporioides, Sclerotinia sclerotiorum, Fusarium oxysporum and Gibberella moniliformis.

Yangbi walnut germin-like protein gene JsGLP1 and application thereof

The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

Method for finishing financial data-handling with video terminal and public payment server

The invention discloses a kind of method to process finance data by visible terminal, said visible terminal connects with the public pay system by condition acceptance subsystem, and said condition acceptance subsystem is used to display the programs which accords with the condition on visual terminal, said condition acceptance subsystem has an intelligent card, said public pay systems stores thecorresponding relationship between the unique identification information with one or several finance accounts, the method includes: the condition acceptance subsystem sends the unique identification information and the relative trading data to the public pay system; and the public pay system sends the relative trading data and the information of finance account to the corresponding processing terminal of finance data according to the corresponding relationship; and the processing terminal of finance data returns the processing result to the public pay system; and the public pay system returnsthe ...

Drip irrigation special fertilizer suitable for whole growth time of cut flower rose

The invention discloses a drip irrigation special fertilizer specially used in the whole growth time of a cut flower rose. The drip irrigation special fertilizer comprises the following main components in part by weight: 442 to 466 parts of potassium nitrate, 115 to 149 parts of mono-ammonium phosphate, 170 to 230 parts of urea, 203 to 211 parts of magnesium sulfate heptahydrate, 0.1 to 0.3 part of boric acid, 3.6 to 5.6 parts of EDTA chelated iron, 0.4 to 0.7 part of EDTA chelated copper, 1.2 to 1.9 parts of EDTA chelated manganese, 0.01 to 0.03 part of EDTA sodium molybdate, and 0.4 to 0.7 part of EDTA chelated zinc. The drip irrigation special fertilizer is specially designed for a drip irrigation system, and can significantly improve the yield and the quality of the rose after being used by combining a law of the demand on various nutrients for the cut flower rose in the whole growth time. Besides, the drip irrigation special fertilizer can also improve the utilization ratio of the ...

Method for synchronously preparing walnut oil and walnut peptide

The invention relates to a technology for synchronously preparing walnut oil and walnut peptide by means of microwave-assisted enzymolysis, belonging to the technical field of the food or the health food. The technology comprises the following steps of: removing the coat and the core of the walnut, grinding the walnut into walnut slurry, adding the water to prepare the slurry, hydrolyzing by adding the protease or the composite protease, treating by microwave, and centrifugally separating the oil phase, the peptide water phase and the residual solid phase, wherein the obtained oil phase is the cleaning and bright walnut oil which can meet the requirement of the green food; and further physically refining the oil phase at low temperature to be taken as the raw material oil of the high-class edible oil, the health food oil, the blend oil and the like. The obtained walnut peptide water solution can be directly used for preparing the degreased walnut peptide beverage by means of the enzyme ...

Black tea extract rich in statins and preparation technique and use thereof

The invention relates to a black tea extract which is rich in statins compounds and the preparation technology and usages thereof. The black tea extract comprises the following main chemical constituents: 10-80 percent of the statins compounds and the balance tea polyphenol, theophyline and tea pigment in terms of dry basis or dry matter content; the physical form can be slime, paste, granula, powder and block. The preparing techniques include the following steps: the black tea is ground, the solution is ultrasonically extracted and centrifugalized, thus the extracted solution and tea residue are obtained; the extracted solution can be obtained by concentration and dryness. The black tea extract preparation technology provide a novel way for the tea deep processing and has the advantages of high added value, extensive application of products, easy obtaining of the crude materials, easy and rapid technology and high production efficiency, etc.

Method for identification and content determination of walnut oil

The invention relates to a method for identification and content determination of walnut oil, which mainly comprises the following operating steps of: (1) manufacturing a sample of mixed oil; (2) manufacturing a detection sample; (3) rapidly measuring the fluorescence spectra of pure walnut oil, pure soybean oil and the detection sample of the mixed oil by using a synchronous fluorescence method; and (4) analyzing and processing spectrum data. The method has easy and simple operation and good effect, the rapid detection of walnut oil content in the mixed sample of the walnut oil and the soybean oil can be realized, and the method is applicable to the quality control field of pure walnut oil and walnut blend oil.

Method for recycling walnut polyphenol and flavone in walnut kernel peeling wastewater through macroporous resin

The invention discloses a method for recycling walnut polyphenol and flavone in walnut kernel peeling wastewater through macroporous resin. The method comprises the operating steps of pretreatment of the walnut kernel peeling wastewater, separation, purification and regeneration of the macroporous adsorption resin, concentration and drying. The concentration of the walnut flavone in the pretreated walnut kernel peeling wastewater is reduced to 0.34mg/mL-0.67mg/mL through dilution, the pH value is adjusted to be 7-9 through concentrated hydrochloric acid, a solution is poured into an adsorption tank filled with the macroporous resin to be adsorbed, the adsorption time is more than 4 hours, after adsorption is finished, desorption is conducted through 60%-80% ethyl alcohol to obtain an ethyl alcohol walnut flavone solution, the solution is processed through vacuum evaporation, concentration and drying, the ethyl alcohol is recycled, and finally fine walnut flavone extract is obtained. The ...

Cactus juice, its preserved one and beverage and prodn. method thereof

The method for preparing preserved cactus and cactus beverage and juice, which features complete nutrients and health-care action, includes such steps as collecting cactus stem, washing and peeling, immersion in lime milk, preserving with sugar and baking for preserved cactus, or collecting cactus stem or fruit, washing and peeling, mashing, filter, mixing, canning and sterilizing for beverage and juice.

Julans sigillata Dode proline-enriched protein gene JsPRP1 and applications thereof

The invention discloses Julans sigillata Dode proline-enriched protein gene JsPRP1 and applications thereof. The nucleotide sequence of the JsPRP1 is represented by the SEQ ID No.1, and the encoding is rich in proline protein. Through the related technologies and researches of functional genomics, the results show that JsPRP1 gene has a function that can improve the plants' performance on resisting fungal infection. The anti-fungus gene JsPRP1 can be built on an expression carrier and over-expressed in tobacco, and the transgene tobacco plants has a strong in-vitro activity on resisting fungi. The transgene tobacco, where the JsPRP1 gene is over-expressed, has a prominent inhibiting effect on the growth of colletotrichum gloesporioides, sclerotinia sclerotiorum, botryosphaeria, and catenulate gibberella.

Beta-1,3-glucanase gene (i)PpGlu(/i) of Pyrus pyrifolia Nakai and application thereof

The invention relates to a beta-1,3-glucanase gene (i)PpGlu(/i) of Pyrus pyrifolia Nakai, with an antifungal activity, and application thereof. The PpGlu gene has a base sequence coded as beta-1,3-glucanase, shown by SEQID. In the invention, the PpGlu gene is proved to have a function of improving antifungal performance of plants by means of technologies related to functional genomics. The antifungal PpGlu gene provided by the invention is structured to a plant expression vector and is transferred into a tobacco for overexpression, and the genetically modified tobacco has a very strong in-vitro antifungal activity. A protein extracting solution of the genetically modified tobacco for expressing the PpGlu gene has different degrees of inhibiting effects on phomopsis fungi, alternaria sp., and fusarium.

Sulphonated Gleditia sinensis Lam polysaccharide and application thereof

The invention relates to a sulphonated Gleditia sinensis Lam polysaccharide with bioactivity and application thereof. The sulphonated Gleditia sinensis Lam polysaccharide is characterized in that a polysaccharide chain contains galactose and mannose, a main chain is D-mannopyranose connected through a beta-(1,4)-glucosidic bond, a branched chain is D-galactopyranose connected through an alpha-(1,6)- glucosidic bond, and the degree of esterification is between 0.1 and 1.7. The sulphonated Gleditia sinensis Lam polysaccharide provided by the invention is obtained by utilizing a sulphonating agent to process a hot water extract of Gleditia sinensis Lam; and the sulphonating method thereof comprises the following steps: adding Gleditia sinensis Lam polysaccharide into the sulphonating agent, reacting while controlling the temperature and stirring, taking out a flask after the reaction is finished, cooling to room temperature, neutralizing by utilizing a NaOH solution, carrying out precipitating ...

Applications of Julans sigillata Dode pathogenesis-related protein 10 (PR-10) gene JsPR10-1

The invention discloses applications of Julans sigillata Dode pathogenesis-related protein 10 (PR-10) gene JsPR10-1. Through the related technologies and researches of functional genomics, the results show that JsPR10-1 gene has a function that can improve the plants' performance on resisting fungal diseases. The anti-fungus gene JsPR10-1 can be built on an expression carrier and over-expressed in tobacco, and the transgene tobacco plants has a strong in-vitro activity on resisting fungi. The transgene tobacco, where the JsPR10-1 gene is over-expressed, has a prominent inhibiting effect on the growth of colletotrichum gloesporioides, sclerotinia sclerotiorum, botryosphaeria, and catenulate gibberella.