07-01-2021 дата публикации
Номер: US20210002194A1
Принадлежит:
A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO. 131-. (canceled)32. A process of isolating 1 ,4-BDO from a fermentation broth comprising removing a portion of solids by disc stack centrifugation to provide a liquid fraction , removing a further portion of solids from the liquid fraction by ultrafiltration , removing a portion of salts from the liquid fraction by nanofiltration , removing a further portion of salts from the liquid fraction by ion exchange , evaporating a portion of water , and distilling 1 ,4-BDO.33. The process of claim 32 , wherein the step of removing a further portion of salts by ion exchange comprises passing the liquid fraction through an ion exchange column selected from the group consisting of a cation exchange column claim 32 , an anion exchange column claim 32 , a mixed-bed ion exchange column claim 32 , and combinations thereof.34. The process of claim 32 , wherein the step of evaporating a portion of water comprises passing the liquid fraction through an evaporator system claim 32 , said evaporator system comprising an effect selected from the group consisting of a falling film evaporator claim 32 , a short path falling film evaporator claim 32 , a forced circulation evaporator claim 32 , a plate evaporator claim 32 , a circulation evaporator claim 32 , a fluidized bed evaporator claim 32 , a rising film ...
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