MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILIARY TREE AND METHODS OF ISOLATING SAME

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

Multipotent stem cells from the extrahepatic billary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILLARY TREE AND METHODS OF ISOLATING SAME

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

MULTIPOTENTIAL CELLS MOTHER OF THE EXTRAHEPATIC BILIARY TREE AND METHODS OF ISOLATION OF THE SAME

Una célula madre multipotencial, poblaciones de células multipotenciales y una poblacion de células multipotenciales enriquecida, cada una presente en el tejido biliar fetal, neonatal, pediátrico y de adultos y hasta 72 horas de post-mortem (aunque con preferencia dentro de las 10 horas de post-mortem) y con capacidad de madurar en multiples tejidos endodérmicos que incluyen los tejidos hepáticos, biliares y pancreáticos. La célula madre/progenitora multipotencial y las poblaciones de células se encuentran en las glándulas peribiliares, y la progenie descendiente de las mismas está presente en todo el árbol biliar, inclusive en la vesícula biliar. Existe una gran cantidad de glándulas peribiliares en las ubicaciones ramificadas del árbol biliar tal como el hilio, el dueto hepático comun, el dueto cístico, el dueto comun, el dueto hepato-pancreático comun y la vesícula biliar. Hay células multipotenciales relacionadas, poblaciones de células multipotenciales y sus progenitores descendientes ...

Multipotent stem cells from the extrahepatic billary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

Multipotent stem cells from the extrahepatic billary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILIARY TREE AND METHODS OF ISOLATING SAME

MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILLARY TREE AND METHODS OF ISOLATING SAME

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

MULTIPOTENT STEM CELLS FROM THE EXTRAHEPATIC BILLARY TREE AND METHODS OF ISOLATING SAME

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands.

BIOMATRIX SCAFFOLDS

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same. 146-. (canceled)47. A method of producing a biomatrix scaffold from biological tissue , comprising the steps of:a) perfusing the biological tissue or homogenizing the biological tissue with a first medium, wherein the osmolality of said first medium is from about 250 mOsm/kg to about 350 mOsm/kg and said first medium is serum free and at neutral pH; thenb) perfusing the biological tissue or extracting the homogenate of step (a) with a delipidating buffer comprising lipases and/or detergents in said first medium; thenc) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from about 2.0M NaCl to about 5.0M, the concentration chosen to keep insoluble collagens identified in the biological tissue; thend) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and thene) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from about 250 mOsm/kg to about 350 mOsm/kg,thereby producing an intact or homogenized biomatrix scaffold from the biological tissue, said biomatrix scaffold comprising at least 95% of the collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue.48. The method of claim 47 , wherein the second medium comprises at least one of the constituents present in interstitial fluid.49. The method of claim 47 , wherein the delipidating buffer comprises from about 20 units/L to about 50 units/L phospholipase A2 and about 1% sodium deoxycholate in the first medium.50. The method of claim 47 , wherein the salt concentration of the buffer of step (c) is from about 3.4M NaCl to about 3.5M NaCl when used ...

BIOMATRIX SCAFFOLDS

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

Biomatrix scaffolds

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

BIOMATRIX SCAFFOLDS

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

Biomatrix Scaffolds

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same. 1. A method of producing a biomatrix scaffold from biological tissue , comprising the steps of:a) perfusing the biological tissue or homogenizing the biological tissue with a first medium, wherein the osmolality of said first medium is from about 250 mOsm/kg to about 350 mOsm/kg and said first medium is serum free and at neutral pH; thenb) perfusing the biological tissue or extracting the homogenate of step (a) with a delipidating buffer comprising lipases and/or detergents in said first medium; thenc) perfusing the tissue or extracting the homogenate of step (b) with a buffer at a neutral pH and comprising a salt concentration from about 2.0M NaCl to about 5.0M, the concentration chosen to keep insoluble collagens identified in the biological tissue; thend) perfusing the tissue or extracting the homogenate of step (c) with RNase and DNase in a buffer; and thene) rinsing the tissue or homogenate of step (d) with a second medium that is at neutral pH, is serum-free and has an osomolality from about 250 mOsm/kg to about 350 mOsm/kg,thereby producing an intact or homogenized biomatrix scaffold from the biological tissue, said biomatrix scaffold comprising at least 95% of the collagens and most collagen-associated matrix components and matrix bound growth factors, hormones and cytokines of the biological tissue.2. The method of claim 1 , wherein the first medium comprises salts claim 1 , minerals claim 1 , amino acids claim 1 , vitamins claim 1 , and sugars.3. The method of claim 1 , wherein the first medium is a basal medium.4. The method of claim 3 , wherein the basal medium is selected from the group consisting of RPMI 1640 claim 3 , DME/F12 claim 3 , DME claim 3 , F12 claim 3 , Waymouth's claim 3 , and William's medium.5. The method of claim 1 , wherein the second ...

Multipotent stem cells from the extrahepatic biliary tree and methods of isolating same

The present invention relates to a multipotent stem cell, multipotent cell populations, and an enriched multipotent cell population, each found in fetal, neonatal, pediatric, and adult biliary tree tissue and up to 72 hours post mortem (although preferentially, within 10 hours post mortem) and capable of maturing into multiple endodermal tissues that include liver, biliary and pancreatic tissues. The multipotent stem/progenitor cell and cell populations are found in peribiliary glands, and progenitors descending from them are present throughout the biliary tree including in the gallbladder. High numbers of the peribiliary glands are found in the branching locations of the biliary tree such as hilum, common hepatic duct, cystic duct, common duct, common hepato-pancreatic duct and gallbladder. Related multipotent cells, multipotent cell populations and their descendent progenitors are found throughout the biliary tree including in the gall bladder, which does not have peribiliary glands. Compositions comprising same, methods of identifying and isolating same, maintaining same in culture, expanding same in culture and differentiating or lineage restricting the same in vitro or in vivo to hepatic, biliary or pancreatic fates (e.g., as hepatocytes, cholangiocytes, and/or pancreatic islet cells) are also provided. Methods of using the multipotent cells and/or multipotent cell populations are also provided.

Biomatrix scaffolds

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, as well as methods of making and using same.

Biomatrix scaffolds

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, as well as methods of making and using same.

バイオマトリックス足場

【課題】バイオマトリックス足場を提供する。【解決手段】本発明は、細胞外マトリックス成分および結合性増殖因子、サイトカインおよびホルモンが富化した組織抽出物であるバイオマトリックス足場を提供するばかりでなく、その製造方法および使用方法も提供する。【選択図】図１

バイオマトリックス足場

【課題】バイオマトリックス足場を提供する。【解決手段】本発明は、細胞外マトリックス成分および結合性増殖因子、サイトカインおよびホルモンが富化した組織抽出物であるバイオマトリックス足場を提供するばかりでなく、その製造方法および使用方法も提供する。【選択図】図１

Andamios de biomatriz

Un método para producir un andamio de biomatriz a partir de tejido hepático de mamífero, que comprende: a) perfundir u homogeneizar el tejido hepático con un primer medio basal, en donde la osmolalidad de dicho primer medio es de 250 mOsm/kg a 350 mOsm/kg y dicho primer medio está libre de suero y a pH neutro; después b) perfundir el tejido biológico o extraer el homogeneizado de la etapa (a) con un tampón de deslipidación que comprende desoxicolato sódico y fosfolipasa A2 en dicho primer medio; después c) perfundir el tejido o extraer el homogeneizado de la etapa (b) con un tampón a un pH neutro y que comprende una concentración de sal de 2,0 M de NaCl a 5,0 M, la concentración elegida para mantener insolubles los colágenos identificados en el tejido biológico; después d) perfundir el tejido o extraer el homogeneizado de la etapa (c) con RNAasa y DNAasa en un tampón; y después e) enjuagar el tejido u homogeneizado de la etapa (d) con un segundo medio que está a pH neutro, está libre de suero y tiene una osmolalidad de 250 mOsm/kg a 350 mOsm/kg, para producir de esta manera un andamio de biomatriz intacto u homogeneizado a partir del tejido biológico, dicho andamio de biomatriz retiene al menos el 95 % de sus colágenos originales y la mayoría de los componentes de la matriz asociados al colágeno y factores de crecimiento, hormonas y citocinas unidos a la matriz del tejido biológico.

Rusztowania biomacierzowe

Andamios de biomatriz

Un método para producir un andamio de biomatriz a partir de un tejido biológico que se selecciona del grupo que consiste en pulmón tiroides, piel, páncreas, vasos sanguíneos, vejiga, riñones, cerebro, árbol biliar, duodeno, aorta abdominal, vena ilíaca, corazón e intestinos, que comprenden: a) perfundir u homogeneizar el tejido biológico con un primer medio basal, en donde la osmolalidad de dicho primer medio es de 250 mOsm/kg a 350 mOsm/kg y dicho primer medio no contiene suero y está a pH neutro; después b) perfundir el tejido biológico o extraer el homogeneizado de la etapa (a) con un tampón delipidante que comprenda desoxicolato de sodio y fosfolipasa A2 en dicho primer medio; después c) perfundir el tejido biológico o extraer el homogeneizado de la etapa (b) con un tampón a un pH neutro y que comprende una concentración de sal de NaCl 2,0 M a 5,0 M, la concentración elegida para mantener los colágenos insolubles identificados en el tejido biológico; después d) perfundir el tejido biológico o extraer el homogeneizado de la etapa (c) con RNasa y DNasa en un tampón y después e) enjuagar el tejido biológico u homogeneizado de la etapa (d) con un segundo medio que está a pH neutro, no contiene suero y tiene una osmolalidad de 250 mOsm/kg a 350 mOsm/kg, lo que produce de esta manera un andamio de biomatriz intacto u homogeneizado a partir del tejido biológico, dicho andamio de biomatriz retiene al menos el 95 % de sus colágenos originales y la mayoría de los mayoría de los componentes de la matriz asociados a colágeno y los factores de crecimiento, hormonas y citocinas unidos a la matriz del tejido biológico.