16-11-2011 дата публикации
Номер: CN0102241767A
Автор:
MEIXIA HE,
SHILIN HE,
HAIYING HUA,
ZHIGANG LI,
LIRONG ZHANG,
HONGMEI ZHANG,
FANG WANG,
YONG ZHANG,
YUANFA GAO,
XIGUO CUI,
LIJING DING,
HE MEIXIA,
HE SHILIN,
HUA HAIYING,
LI ZHIGANG,
ZHANG LIRONG,
ZHANG HONGMEI,
WANG FANG,
ZHANG YONG,
GAO YUANFA,
CUI XIGUO,
DING LIJING
Принадлежит:
The invention relates to an extraction method of bovine serum albumin, aiming to solve the technical problems of complexity, low purity, high production cost and difficulty in large-lot production of the production process of bovine serum albumin. The extraction method comprises the following steps of: adding a proper amount of sodium palmitate to the separated serum, standing, regulating the pH of the solution, heating in water bath for 1-2 hours, filtering, sequentially adding 3MHCl and sodium chloride to the filtrate, standing at 4 DEG C, filtering to obtain precipitate, adding a proper amount of water to the precipitate, regulating the pH for dissolving the precipitate, then regulating the pH of the solution to 8.0-9.0, dialyzing and lyophilizing. The extraction method of bovine serumalbumin has low process materials input, short process flow, simplicity of operation, high production efficiency and suitability for mass production, and the obtained product has high purity and goodquality ...
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