Microfluidic point-of-care assay

Integrated fluid sample test strip 100 with an inlet 102 for a fluid sample and substrate solution. The inlet has a retention valve 104 for temporarily retaining each solution and reducing air flow past the valve; this may reduce dead volumes in the strip. A reaction chamber 106 receives solutions from the inlet via the retention valve and comprises bioreceptors for binding to an analyte. A capillary pump 110 receives solutions from the reaction chamber and comprises at least one vent hole 112 for air to escape the pump. A hydrophobic vent hole 118 couples to a test chamber comprising electrodes for biosensing; when the hydrophobic vent is unsealed, solution flows from the reaction chamber into the test chamber or, when sealed, into the capillary pump instead. The strip may measure hormone levels, such as cortisol levels in saliva, and may be used to perform an ELISA or ELONA test.

Performance of an analyzer for biological samples

An apparatus ( 11 ) for processing a sample, circuit board ( 8008 ) suited to use in such an apparatus and a method of processing are provided in which the apparatus ( 11 ) is provided with alignment features to assist in provided the circuit board and a sample cartridge ( 9 ) in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge ( 9 ) are positioned against a plurality of elements, such as a heater ( 4 ) for heating the sample cartridge ( 9 ) or an electrical contact (EP1-EP4) for powering features on the sample cartridge ( 9 ), correctly and can operate independently of one another.

Performance

Instruments, devices and methods of analysis are provided which fully integrate a significant number of process steps in a continuous operation. Accurate positioning and full contact between components is also provided by the relative movement the designs allow. An effect interface between a low cost disposable cartridge or device and the instrument to process it is also detailed.

METHOD AND SYSTEM FOR ANALYZING A SAMPLE

A method and system for analysing a sample are provided, wherein one or more process steps and/or sample processors are provided separately from the instrument, for instance a sample receiving step and sample preparation step and sample extraction step and sample retention step and/or purification step and washing step and elution step, and one or more process steps and/or sample processors provided by the instrument as an integrated set, the one or more process steps and/or sample processors provided by the instrument including a sample receiving step and amplification step and denaturing step and investigation step and detection step and results analysis step and results output step. Other combinations of the split in location of the steps are possible. The optimisation of the split allows the accurate processing by a cartridge based instrument of the sample, whilst fully interfacing with a variety of sample collection and/or preparation approaches. 1. A method of analysing a sample , the method comprising: applying one or more process steps to the sample; and obtaining one or more results from the method; wherein an instrument provides some of a set of process steps and/or sample processors and one or more process steps and/or sample processors are provided separately from the instrument.2. A method according to claim 1 , wherein the one or more process steps and/or sample processors provided separately from the instrument include one or more of: a sample receiving step and/or sample preparation step and/or sample extraction step and/or sample retention step and/or purification step and/or washing step and/or elution step.3. A method according to claim 1 , wherein the one or more process steps and/or sample processors provided by the instrument include one or more of: a further sample receiving step and/or amplification step and/or PCR step and/or denaturing step and/or investigation step and/or detection step and/or electrophoresis step and/or analysis step and/ ...

RELATING TO DEVICES

A method of analysis, instrument for analysis and device for use in such an instrument are provided, which perform a number of processes need to reach a useful result in the context of a wide variety of samples. The sequence of those processes being optimised. A device, instrument using the device and method of use are also provided which offer reliable performance of a heating based process, with minimal condensation and/or sample loss issues.

ANALYSIS

A method of analysing a sample includes providing a first part of the sample and a second part of the sample. A first analysis is conducted on the first part of the sample and the results of the first analysis are considered. A second analysis is conducted on the second part of the sample, the second analysis being conducted according to a procedure using a value for each of one or more characteristics of the procedure. The consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value. The second analysis is started before the results of the first analysis are obtained. 1. A method of analysing a sample , the method including:providing a first part of the sample;providing a second part of the sample;conducting a first analysis on the first part of the sample;considering the results of the first analysis;conducting a second analysis on the second part of the sample, the second analysis being conducted according to a procedure, the procedure using a value for each of one or more characteristics of the procedure;wherein the consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value; andwherein the second analysis is started before the results of the first analysis are obtained.2. The method of claim 1 , wherein the first analysis and the second analysis start within 5 minutes of the other analysis starting.3. The method of wherein the first analysis takes less than 60 minutes to complete.4. The method of wherein the first analysis is completed at least 30 minutes before the scheduled completion of the second analysis.5. The method of wherein the first part and the second part have the same volume +/−1%.6. The method of wherein the ratio of sample to reagents is the same in respect of the first part and in respect of the second part +/−1%. ...

Method and system for biological information pattern storage and readout

Provided herein are biological information pattern (BIP) arrays and related methods for reading out information stored in a biological medium. In this manner, encoded digital information in biomolecular medium can be used as a high data density storage medium that may be read-out and accessed in a label-free manner.

PERFORMANCE

Instruments, devices and methods of analysis are provided which fully integrate a significant number of process steps in a continuous operation. Accurate positioning and full contact between components is also provided by the relative movement the designs allow. An effect interface between a low cost disposable cartridge or device and the instrument to process it is also detailed.

ANALYSIS

Analysis methods and apparatus are provided for inspecting a channel, such as a capillary electrophoresis channel, in a device. Configuration and alignment systems are provided, together with optical systems and temperature control.

Method and System for Biological Information Pattern Storage and Readout

Provided herein are biological information pattern (BIP) arrays and related methods for reading out information stored in a biological medium. In this manner, encoded digital information in biomolecular medium can be used as a high data density storage medium that may be read-out and accessed in a label-free manner. 1. A method of retrieving digital information stored in a biological system , the method comprising the steps of:translating digital information to a biomolecular medium;providing a microarray comprising a substrate receiving surface and a plurality of unique substrate-bound sequences in a selected pattern;depositing a liquid droplet, or an array of liquid droplets, to the substrate receiving surface, wherein the liquid droplet comprises the biomolecular medium;drying the liquid droplet(s) on the substrate receiving surface to form a progressive recession of a wetting line and a corresponding dried residue pattern, wherein the recession of the wetting line and corresponding dried residue pattern is influenced by an interaction or a lack of interaction between the biomolecular medium and the plurality of unique substrate-bound sequences to form a biological information pattern (BIP);imaging the dried residue pattern with an imager; andprocessing the image of the dried residue pattern with a processer to thereby decode digital information stored in the biomolecular medium.2. The method of claim 1 , wherein the biomolecular medium comprises a nucleic acid molecule.3. The method of claim 1 , that is label-free.4. The method claim 1 , wherein the imaging step is by a sensor in a hand-held or mobile device.5. The method of claim 1 , wherein the biomolecular medium comprises three regions:a bead-binding sequence region;a substrate-binding sequence region; anda digital information region positioned between the bead-binding and substrate-binding regions.6. The method of claim 1 , wherein the biomolecular medium comprises a nucleic acid sequence claim 1 , an amino ...

COMPONENTS

A method and device structure are provided which enable an archive sample to be collected and detached relative to a device within which a series of processes, such as PCR are being provided. A chamber structure and method of use are provided in which a controlled and precise volume is obtained by control of the relative resistance to flow through various channels.

SYSTEMS AND METHODS FOR IDENTIFICATION OF FLUID AND SUBSTRATE COMPOSITION OR PHYSICO-CHEMICAL PROPERTIES

Techniques for identifying a composition of a target fluid using a set of vectors representing known residue patterns for a two or more fluids including said target fluid is provided. An exemplary method includes storing one or more digital measurements of residue for the target fluid, extracting one or more descriptive features from the measurements; and processing descriptive features to identify the composition of the target fluid. The processing includes using a machine learning algorithm trained with data linking residue morphology to fluid composition. A distance between a vector representing said one or more descriptive features and said set of vectors representing known residue patterns is determined, and a residue is assigned to one or more of the known residue patterns. 1. A method for identifying at least one of a physico-chemical property or composition of a target fluid using a set of vectors representing known residue patterns for a two or more fluids including said target fluid , comprising:acquiring one or more digital measurements of residue for said target fluid;extracting one or more descriptive features from at least one of said measurements; andprocessing one or more of said descriptive features to identify said at least one physico-chemical property or composition of said target fluid from said at least one of said measurements;wherein said processing includes using a matching algorithm or a machine learning algorithm trained with data linking residue patterns to fluid composition or physico-chemical properties.2. The method of claim 1 , further comprising:determining a distance between a vector representing said one or more descriptive features and said set of vectors representing known residue patterns, andassigning a residue to one or more of the known residue patterns that minimizes said distance.3. The method of claim 1 , wherein acquiring one or more digital measurements comprises capturing one or more images.4. The method of claim 1 , ...

ANALYSIS

A method of analysing a sample includes providing a first part of the sample and a second part of the sample. A first analysis is conducted on the first part of the sample and the results of the first analysis are considered. A second analysis is conducted on the second part of the sample, the second analysis being conducted according to a procedure using a value for each of one or more characteristics of the procedure. The consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value. The second analysis is started before the results of the first analysis are obtained.

Sensing And Identifying Biological Samples On Microfluidic Devices

A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction. 1. A method for analysis of a biological sample comprising:receiving the sample at a sample collection chamber, wherein the sample includes deoxyribonucleic acid (DNA);automatically transporting the sample to a lysis unit;automatically operating the lysis unit to cause lysis to obtain access to the DNA;automatically transporting the DNA to a purification unit;automatically operating the purification unit to isolate the DNA from other components in the sample;automatically transporting the isolated DNA to an amplification unit;automatically operating the amplification unit to amplify the DNA;automatically transporting fragments of the amplified DNA to a separation unit;automatically operating the separation unit to separate the DNA into fragments;automatically operating a detection unit to detect the fragments using laser induced fluorescence; ...

DEVICES

A method of analysis, instrument for analysis and device for use in such an instrument are provided, which perform a number of processes need to reach a useful result in the context of a wide variety of samples. The sequence of those processes being optimised. A device, instrument using the device and method of use are also provided which offer reliable performance of a heating based process, with minimal condensation and/or sample loss issues. 1. A device , for processing a sample , the device including:one or more sample processors, wherein the sample processors include a sample preparation step, sample extraction step, sample retention step, elution step, amplification step, denaturing step, detection step and results output step.2. A device according to in which the device is a cartridge.3. A device according to in which the device includes a sample processor for one or more of a sample receiving step claim 1 , purification step claim 1 , washing step claim 1 , PCR step claim 1 , investigation step claim 1 , analysis step.4. A device according to in which the sample receiving step includes the transfer of a sample from outside the device to inside the device claim 1 , the sample receiving step receiving the sample from a collection device or from a storage device.5. A device according to in which the sample preparation step includes contacting the sample with one or more reagents.6. A device according to the sample retention step includes contacting the sample with one or more reagents and/or components which retain the sample component(s) relative to one or more waste components in the sample.7. A device according to in which the sample component(s) are retained on one or more magnetic beads.8. A device according to in which the waste component(s) are removed from the retained sample components by being washed away from the retention step using one or more further reagents and/or components.9. A device according to in which the retained and/or selected sample ...

ANALYSIS

Analysis methods and apparatus are provided for inspecting a channel, such as a capillary electrophoresis channel, in a device. Configuration and alignment systems are provided, together with optical systems and temperature control. 1. A method of configuring an optical system for a detection location , the method including: 'considering one or more characteristics of the channel and/or optical system;', 'providing a channel having a detection location for performing an electrophoretic separation on at least a part of a sample;'}configuring the optical system relative to the detection location.2. A method according to in which the optical system is provided in an instrument claim 1 , the channel is provided in a device which can be inserted into and removed from the instrument and the configuring of the optical system is made before the electrophoretic separation.3. A method according to in which the configuring of the optical system is made after one or more other steps have been performed on the device.4. A method according to in which the configuring is with respect to the position of one or more parts of the optical system relative to the channel.5. A method according to in which the position is the position perpendicular to the axis of the channel claim 4 , parallel to the axis along which light is applied to the detection location.6. A method according to in which the position is the position perpendicular to the axis of the channel claim 4 , perpendicular to the axis along which light is applied to the detection location.7. A method according to in which the configuring is with respect to the focus of the light applied to the detection location.8. A method according to in which the configuring is made with reference to the channel claim 1 , the channel is responsive to light and the variation in the detected light with position across the channel is considered.9. A method according to in which one or more components of the optical system are moved and/or the ...

PERFORMANCE OF AN ANALYSER FOR BIOLOGICAL SAMPLES

An apparatus () for processing a sample, circuit board () suited to use in such an apparatus and a method of processing are provided in which the apparatus () is provided with alignment features to assist in provided the circuit board and a sample cartridge () in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge () are positioned against a plurality of elements, such as a heater () for heating the sample cartridge () or an electrical contact (EP1-EP4) for powering features on the sample cartridge (), correctly and can operate independently of one another. 1. An apparatus for processing a sample , the apparatus being provided with a circuit board and being provided with , in use , a sample cartridge , the circuit board interfacing with a sample cartridge in use , wherein the sample cartridge comprises a working surface defining a plurality of sections for processing a sample; wherein the circuit board is configured to be adjacent the working surface in use and comprises:a plurality of elements, each of the plurality of elements is associated with one of the plurality of sections and is arranged to be positioned adjacent the associated one of the plurality of sections in use; andwherein the circuit board further delimits at least one alignment feature for engaging a corresponding alignment feature on the cartridge and ensuring that the plurality of elements are positioned adjacent the associated one of the plurality of sections in use.2. Apparatus according to claim 1 , wherein one or more elements are provided and the elements are selected from the group comprising: a heater for heating the sample cartridge claim 1 , an electrical contact for powering features on the sample cartridge claim 1 , an electrical contact for powering a pump on the sample cartridge.3. Apparatus according to claim 1 , wherein the sample cartridge provides a plurality of sections and the circuit board enables these ...

PERFORMANCE

Instruments, devices and methods of analysis are provided which fully integrate a significant number of process steps in a continuous operation. Accurate positioning and full contact between components is also provided by the relative movement the designs allow. An effect interface between a low cost disposable cartridge or device and the instrument to process it is also detailed. 2. An instrument according to in which the relative movement for the device is provided to align the device with a device to instrument interface and/or to contact the device with a device to instrument interface.3. An instrument according to in which the relative movement for the device is provided to align the device with an investigation step and/or detection step and/or electrophoresis step and/or analysis step.4. An instrument according to in which the device is provided on a carrier.5. An instrument according to in which the relative movement is used to move the device from a insertion position to a use position.6. An instrument according to in which the relative movement for the device is made in response to one or more alignment checks conducted by the instrument.7. An instrument according to in which one or more releasable fasteners hold the device against the carrier claim 1 , but allow movement of the device away from the carrier in response to a force above a certain level.8. An instrument according to in which when the force is removed and/or reduced below that level claim 7 , the device returns to the insertion position and/or into contact with the carrier.9. An instrument according to in which the carrier interacts with the instrument and/or casing and/or device location and/or device to instrument interface to position the device relative to the device to instrument location.10. An instrument according to in which the device is moved from the insertion position to a use position in which the device contacts the device to instrument interface.11. An instrument according to ...

Method and Device for Generating a Tunable Array of Fluid Gradients

Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties. 1. A microfluidic gradient generator for tuning dynamic components of fluid comprising:a first fluid conduit;a second fluid conduit,an intersection region that fluidically connects the first fluid conduit and the second fluid conduit, the intersection region comprising an intersection opening between the first fluid conduit and the second fluid conduit and a flow-divider that extends in a downstream direction from the intersection opening;a source reservoir channel fluidically connected to the intersection region and extending downstream from the intersection opening;a sink reservoir channel fluidically connected to the intersection region and extending downstream from the intersection opening;a microchannel array comprising a plurality of microchannels, each microchannel having an inlet end connected to the source reservoir channel and an outlet end connected to the sink reservoir channel, wherein adjacent microchannels are separated from each other by a separation distance, wherein the microchannel array traverses an axial distance along the source ...

COMPONENTS

A method and device structure are provided which enable an archive sample to be collected and detached relative to a device within which a series of processes, such as PCR are being provided. A chamber structure and method of use are provided in which a controlled and precise volume is obtained by control of the relative resistance to flow through various channels. 112.-. (canceled)13. A microfluidic device , the microfluidic device having:a) an entry location for a biological sample for analysis;b) a channel connected to the entry location;c) a receiving location, the receiving location being connected to the channel;wherein the receiving location is detachable from the microfluidic device;wherein the receiving location is an integral part of the microfluidic device;wherein the receiving location is in a section of the microfluidic device and an area or line of weakness is between the microfluidic device and the section; andone or more processing locations between the entry location and the receiving location, wherein the one or more processing locations are channels and/or chambers and the processing locations include one or more of: a mixing location, a washing location, a selective separation location for DNA from one or more other material, an amplification process location, a location at which a magnetic field is applied and/or removed and/or varied, a cell lysis location, a surface based reaction location, a PCR reaction chamber connected to the receiving location.1415.-. (canceled)16. A microfluidic device according to in which the microfluidic device is provided with a first valve in the section for sealing the channel between the receiving location and the part of the channel disrupted when the section is detached from the microfluidic device.17. A microfluidic device according to in which the microfluidic device is provided with a second valve not on the section for sealing the channel between the part of the channel disrupted when the section is detached ...

COMPONENTS

A method and device structure are provided which enable an archive sample to be collected and detached relative to a device within which a series of processes, such as PCR are being provided. A chamber structure and method of use are provided in which a controlled and precise volume is obtained by control of the relative resistance to flow through various channels. 1. A method of providing a storable sample , the method including:a) introducing a sample to a device;b) conveying at least a part of the sample to a receiving location to provide a storable sample, wherein a storable sample of a sample on which one or more processes and/or reactions are performed is provided;wherein the receiving location is provided in a section of the device that is detachable from the device; andwherein the receiving location is an integral part of the device.2. (canceled)3. A method according to in which the method further includes the section being detached from the device by breaking the material joining the section and the device.4. A method according to in which the storable sample is provided before one or more processes and/or reactions are performed.5. A method according to in which the storable sample is provided after one or more processed and/or reactions are performed.6. A method according to in which the one or more processes and/or one or more reactions include one or more of: cell lysis claim 5 , mixing claim 5 , a surface based reaction claim 5 , washing claim 5 , elution claim 5 , selective separation of DNA from one or more other materials claim 5 , application of a magnetic field claim 5 , removal of a magnetic field claim 5 , amplification claim 5 , PCR claim 5 , detection and denaturation.7. A method according to in which the method provides a storable sample which is one part of the sample and one or more other parts of the sample are used in one or more processes and/or reactions.8. A method according to in which the method includes one or more of the following ...

Sensing and identifying biological samples on microfluidic devices

A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction.

ANALYSIS

A method of analysing a sample includes providing a first part of the sample and a second part of the sample. A first analysis is conducted on the first part of the sample and the results of the first analysis are considered. A second analysis is conducted on the second part of the sample, the second analysis being conducted according to a procedure using a value for each of one or more characteristics of the procedure. The consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure is changed to a different value. The second analysis is started before the results of the first analysis are obtained. 1. A method of analysing a biological sample , the method including:providing a first part of the biological sample;providing a second part of the biological sample;conducting computer implemented a first analysis on the first part of the biological sample to provide results of the first analysis;considering the results of the first analysis;conducting a computer implemented second analysis on the second part of the biological sample, to provide results of the second analysis, the second analysis being conducted according to a procedure, the procedure using a value for each of one or more characteristics of the procedure;wherein the computer implemented consideration of the results of the first analysis is used to determine whether the value for one or more of the characteristics of the procedure by which the second analysis is conducted is changed to a different value; the second analysis being conducted and providing the results of the second analysis using the procedure for the second analysis that incorporates the different value for the one or more characteristics; andwherein the second analysis is started before the results of the first analysis are obtained.2. The method of claim 1 , wherein the first analysis and the second analysis start within 5 minutes of the other analysis ...

ELECTROPHORESIS SYSTEM

An electrophoresis device where the end of the electrophoresis channel has a part that extends vertically. The electrophoresis channel includes a first access port at one end of the channel and a second port at the other end of the channel, which are in the same horizontal plane. In this way, there is a less than 0.1% hydrostatic pressure differential between two ports and the performance of the device is improved. 1. A device , for processing a sample , the device comprising: an electrophoresis step provided on the device , the electrophoresis step including one or more channels including an electrophoresis channel which includes a separation length and a further part at one or both ends , one or both of the further parts , at least in part , extend in the direction of gravity.2. A device according to claim 1 , wherein one or both of the further parts have a vertical portion.3. A device according to wherein the electrophoresis channel has a first end access location towards one end of the channel and the electrophoresis channel has a second end access location towards the other end of the channel claim 1 , the first end access location and the second end access location being provided in the same horizontal plane.4. A device according to wherein there is a less than 0.1% hydrostatic pressure differential between the first end access location and the second end access location.5. A device according to wherein the electrophoresis channel has a side channel claim 1 , the side channel is connected to the sample feed channel and the electrophoresis channel has a second side channel.6. A device according to wherein the first side channel extends claim 5 , at least in part claim 5 , in the direction of gravity claim 5 , to the electrophoresis channel.7. A device according to wherein the second side channel claim 5 , at least in part claim 5 , extends in the direction of gravity.8. A device according to wherein the second side channel has a first vertical portion and a ...

PERFORMANCE OF AN ANALYSER FOR BIOLOGICAL SAMPLES

An apparatus for processing a sample, circuit board suited to use in such an apparatus and a method of processing in which the apparatus has alignment features to assist in aligning the circuit board and a sample cartridge in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge are positioned against a plurality of elements, such as a heater for heating the sample cartridge or an electrical contact (EP-EP) for powering features on the sample cartridge, correctly and can operate independently of one another. 111.-. (canceled)12. A circuit board for interfacing with a sample cartridge in use , wherein the sample cartridge comprises a working surface defining a plurality of sections for processing a sample; wherein the circuit board is configured to be adjacent the working surface in use and comprises:a plurality of elements, each of the plurality of elements is associated with one of the plurality of sections and is arranged to be positioned adjacent the associated one of the plurality of sections in use; andwherein the circuit board further delimits at least one alignment feature for engaging a corresponding alignment feature on the cartridge and ensuring that the plurality of elements are positioned adjacent the associated one of the plurality of sections in use.13. A circuit board according to claim 12 , wherein one or more elements are provided and the elements are selected from the group comprising: a heater for heating the sample cartridge claim 12 , an electrical contact for powering features on the sample cartridge claim 12 , an electrical contact for powering a pump on the sample cartridge.14. A circuit board according to claim 12 , wherein the sample cartridge provides a plurality of sections and the circuit board enables these sections to be activated as required for processing the sample by the at least one alignment feature ensuring that the features on the circuit board are aligned ...

ANALYSIS INSTRUMENT HAVING A SAMPLE BEARING DEVICE LOCATION

Instruments, devices and methods of analysis are provided which fully integrate a significant number of process steps in a continuous operation. Accurate positioning and full contact between components is also provided by the relative movement the designs allow. An effect interface between a low cost disposable cartridge or device and the instrument to process it is also detailed. 2. An instrument according to in which the relative movement for the device is provided to align the device with a device to instrument interface.3. An instrument according to in which the relative movement for the device is provided to align the device with a step selected from the group comprising: an investigation step; a detection step; an electrophoresis step; an analysis step.45.-. (canceled)6. An instrument according to in which the relative movement for the device is made in response to one or more alignment checks conducted by the instrument.710.-. (canceled)11. An instrument according to in which movement to the use position and/or a subsequent step provide for the formation of contact between the part of the device providing an electrophoresis step with a heater board.1321.-. (canceled)22. An instrument according to claim 1 , wherein movement to the use position provides formation of electrical contacts between the instrument and one or more electrical components provided on the device.23. An instrument according to claim 3 , wherein movement to the use position provides formation of electrical contacts between the instrument and one or more electrical components provided on a part of the device providing the electrophoresis step.24. An instrument according to claim 11 , wherein movement to the use position provides formation of electrical contacts between the instrument and one or more electrical components provided on a part of the device providing the electrophoresis step.25. An instrument according to claim 1 , in which movement to the use position provides deactivation of ...

Improvements in and relating to performance of an analyser for biological samples

An apparatus (11) for processing a sample, circuit board ( 8008 ) suited to use in such an apparatus and a method of processing are provided in which the apparatus (11) is provided with alignement features to assist in provided the circuit board and a sample cartridge (9) in the correct position relative to one another. In this way, the plurality of sections for processing a sample in the cartridge (9) are positioned against a plurality of elements, such as a heater (4) for heating the sample cartridge (9) or an electrical contact (EP1 -EP4) for powering features on the sample cartridge (9), correctly and can operate independently of one another.

Sensing and identifying biological sampels on microfluidic devices

A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction.

Method and device for generating a tunable array of fluid gradients

Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties.

Improvements in and relating to analysis

The method is provided for improving results achieved from an analysis sample, by conducting a parallel analysis of the same sample and using the results from that analysis to control, modify or alter the main analysis. This is particularly useful in the context of analysis of DNA containing samples. The feedback may alter the conditions used, duration of the analysis or steps therein or other variables.

Sensing and identifying biological sampels on microfluidic devices

A method, system, and apparatus for analysis of a biological sample includes receiving the sample, wherein the sample includes deoxyribonucleic acid (DNA), lysing the sample to obtain access to the DNA included in the sample, purifying the DNA in the sample to isolate the DNA from other components in the sample, amplifying the DNA, separating fragments of the amplified DNA, detecting the separated fragments using laser induced fluorescence, based on the detecting, generating a profile of the DNA in the received sample, comparing the generated profile with profiles of DNA stored in a database, and upon determining that the generated profile matches one of the stored profiles, identifying the source from which the stored profile was obtained, wherein the receiving, lysing, purifying, amplifying, and detecting are performed on corresponding portions of a microfluidic device, and wherein transporting the sample and the DNA to the portions of the microfluidic device and enabling the lysing, purifying, amplifying, separating, detecting, generating, comparing, and identifying are performed automatically without user interaction.

Microfluidic collection and test

An integrated fluid sample test strip 200 comprising a first layer 224, an inlet 202 for receiving a series of solutions including a fluid sample and a substrate solution, a test chamber 206 and a second layer 222. The test chamber incubates and performs biosensing tests on one or more solutions received from the inlet and is functionalized with one or more bioreceptors for binding to a target analyte wherein the test chamber comprises a plurality of test electrodes 216 to perform at least part of a biosensing test and is configured to receive the solutions from the inlet. The second layer comprises a cavity corresponding to the test chamber, wherein the cavity is recessed along and under at least part of a sidewall of the test chamber, the second layer comprising a hydrophilic surface adjacent to the first layer. The second layer may comprise a channel for guiding a solution from the inlet to the test chamber wherein the channel is formed in the hydrophilic surface of the second layer. The strip may also comprise a capillary pump 210 and vent holes 212. Ideally, the strip is used to perform ELISA or ELONA tests for hormones on saliva.

Integrated microfluidic test strip

A test device (10) for bodily fluids is disclosed. The test device comprises: at least one reagent port configured to accept at least one reagent under pressure from a reagent pouch; a sample port configured to accept a bodily fluid sample; a common port, com¬ prising an output and at least one input, each at least one input of the common port coupled to one reagent port of the at least one reagent port by a reagent channel, the output of the common port coupled to the sample port by a transfer channel; and at least one reaction chamber comprising a plurality of electrodes to perform at least part of a biosensing test of a substrate solution, the substrate solution comprising the bodily fluid sample and the at least one reagent, the reaction chamber functionalized with one or more bioreceptors for binding to a target analyte, each reaction chamber of the at least one reaction chamber coupled to the sample port by a retention channel, wherein a top surface of the common port comprises a gas-permeable membrane configured to allow gas to pass through but prevent fluid.

Improvements in and relating to analysis

The method is provided for improving results achieved from an analysis sample, by conducting a parallel analysis of the same sample and using the results from that analysis to control, modify or alter the main analysis. This is particularly useful in the context of analysis of DNA containing samples. The feedback may alter the conditions used, duration of the analysis or steps therein or other variables.

Microfluidic collection and test

An integrated fluid sample test strip comprising a first layer, an inlet for receiving a series of solutions, said solutions comprising at least a fluid sample and a substrate solution and a test chamber for incubating and performing biosensing tests on one or more solutions received from the inlet, the test chamber formed in the first layer and configured to receive the solutions from the inlet. The test chamber is functionalized with one or more bioreceptors for binding to a target analyte, and comprises a plurality of test electrodes to perform at least part of a biosensing test of the substrate solution. The test strip further comprises: a second layer comprising a cavity that is adjacent to the test chamber, wherein the cavity is recessed along and under at least part of a sidewall of the test chamber, the second layer comprising a hydrophilic surface adjacent to the first layer; and/or a channel for guiding a solution from the inlet to the test chamber, wherein the channel is formed in at least the hydrophilic surface of the second layer.

Microfluidic point-of-care assay

The disclosure describes an integrated fluid sample test strip comprising: an inlet for receiving solutions comprising a fluid sample and a substrate solution, the inlet comprising a retention valve for temporarily retaining each solution to thereby reduce air flow through the valve; a reaction chamber to receive the solutions via the retention valve, the chamber functionalized with bioreceptor(s); a capillary pump to receive from the reaction chamber the solution(s), the pump comprising vent hole(s); a test chamber to receive the substrate solution from the reaction chamber, the test chamber comprising test electrodes for a biosensing test of the substrate solution; a hydrophobic vent hole coupled to the test chamber to allow a flow of solution from the reaction chamber into the test chamber when the vent hole is unsealed and to allow a flow of solution from the reaction chamber to the capillary pump when the vent hole is sealed.

Microfluidic point-of-care assay

The disclosure describes an integrated fluid sample test strip comprising: an inlet for receiving solutions comprising a fluid sample and a substrate solution, the inlet comprising a retention valve for temporarily retaining each solution to thereby reduce air flow through the valve; a reaction chamber to receive the solutions via the retention valve, the chamber functionalized with bioreceptor(s); a capillary pump to receive from the reaction chamber the solution(s), the pump comprising vent hole(s); a test chamber to receive the substrate solution from the reaction chamber, the test chamber comprising test electrodes for a biosensing test of the substrate solution; a hydrophobic vent hole coupled to the test chamber to allow a flow of solution from the reaction chamber into the test chamber when the vent hole is unsealed and to allow a flow of solution from the reaction chamber to the capillary pump when the vent hole is sealed.

Dispositif de séquençage d’une sequence de nucleotides présentant une sensibilité augmentée et une fiabilité améliorée

Dispositif de séquençage d’au moins un brin de nucléotides comportant un support (3), au moins une partie mobile (4) par rapport au support (3), des moyens de mise en vibration de la partie mobile (4) à une fréquence donnée, des moyens de mesure de la fréquence de vibration de la partie mobile (4), une sonde de reconnaissance (SR) reliant mécaniquement le support et la partie mobile (4), la sonde de reconnaissance (SR) comportant au moins une séquence de nucléotides. Figure pour l’abrégé : 1.

Integrated microfluidic test strip

Test device [10, Fig. 1] comprising: at least one reagent port [22, Fig 2a] accepting at least one reagent under pressure from a reagent pouch; a sample port 21 for a bodily fluid sample; a common port 23 comprising an output and at least one input, each input coupled to one reagent port by a reagent channel 24, the output of the common port joined to the sample port by a transfer channel [41, Fig 4a]; and at least one reaction chamber 25. The reaction chamber comprises a plurality of electrodes to perform at least part of a biosensing test of a substrate solution comprising the bodily fluid sample and the reagent and has one or more bioreceptors for binding to a target analyte. Each reaction chamber is coupled to the sample port by a retention channel. A top surface of the common port comprises a gas-permeable membrane configured to allow gas to pass through but prevent fluid.

Microfluidic point-of-care assay