Method for in-situ determination of wood fiber biomass enzyme accessibility

The invention belongs to the field of environmental biological energy and relates to a method for in-situ determination of cellulase accessibility of wood fiber biomass. The method comprises the following steps of: 1) marking general enzyme binding sites of a wood fiber biomass sample; 2) in-situ observing the distribution and the quantity of the general enzyme binding sites, and calculating to obtain the quantity of fiber globule fluorescent protein probes which are attached onto the surface of the sample; 3) releasing the general enzyme binding sites; 4) closing the sample obtained in the step 3 by using lignin attachment proteins, and marking the cellulase binding sites in the sample to obtain the distribution and the quantity of the cellulase binding sites and the quantity of the fiber globule fluorescent protein probes which are attached onto the surface of the sample; and 5) comparing results of the step 4 and the step 2, and evaluating the cellulase accessibility of the wood fiber ...