Genetically engineered bacteria for producing proline aminopeptidase and construction method thereof

The invention discloses genetically engineered bacteria for producing proline aminopeptidase and a construction method thereof, belonging to the technical field of bioengineering. The genetically engineered bacteria are obtained by introducing the mature-coding proline aminopeptidase gene from aspergillus oryzae JN-412 (CGMCC No.8474) into E.coli BL21 (DE3). The genetically engineered bacteria can efficiently express the proline aminopeptidase, the enzyme activity in the supernate of fermentation liquid is 25.87U/mL, the specific enzyme activity is 40.87U/mg, and the required protein is provided to later study of the characteristics, structure and the like of proline aminopeptidase.

Regenerative burner capable of completely burning coal gas

The invention relates to a regenerative burner capable of completely burning coal gas, and belongs to the technical field of regenerative heating furnace equipment. The regenerative burner is used for completely burning residual coal gas in the regenerative burner. According to the technical scheme, the regenerative burner is of an air-coal gas dual-regenerative structure; a nitrogen purging device is arranged at the lower part of a coal gas three-way valve; after the regenerative burning process is finished, a high-speed nitrogen switching valve on a nitrogen purging pipeline is switched on to purge residual coal gas between the coal gas three-way valve and a coal gas regenerative burner body into a furnace by virtue of nitrogen, the purging time is adjusted according to the volume of the residual coal gas, and the complete burning is realized; in the regeneration process, the high-speed nitrogen switching valve is switched off, and the regenerative burner normally regenerates. According ...

Heat accumulative roller bottom heating furnace

The invention relates to a heat accumulative roller bottom heating furnace, which belongs to the technical field of steel rolling equipment. Through the application of the heat accumulative combustion technology, the heating quality of steel blanks is improved, and the coal gas consumption is reduced. The invention has the technical scheme that the heat accumulative roller bottom heating furnace comprises a furnace body, a furnace hearth and heat accumulative burner nozzles, wherein the heat accumulative roller bottom heating furnace comprises a plurality of heat accumulative burner nozzles which are uniformly distributed on the upper layers of both sides of the furnace in the furnace length direction, coal gas spray openings and air spray openings are arranged in the heat accumulative burner nozzles, the coal gas spray openings are distributed in the center of the heat accumulative burner nozzles, the air spray openings are uniformly distributed in the peripheral direction of the coal ...

Single air regenerative burner for roller hearth furnace

The invention discloses a single air regenerative burner for a roller hearth furnace, which belongs to the technical field of regenerative heating furnace equipment and is used for heating plate blanks. According to the technical scheme, air and smoke are subjected to heat storage and heat discharge in an upper box body, a coal gas spray gun is mounted in a lower box body, the central line of the coal gas spray gun coincides with that of a burner brick, and an air spraying hole and a coal gas spraying hole are formed in the burner brick. When the single air regenerative burner is used, coal gas and the air are sprayed out to form a concentric jet-flow state and are premixed and burnt in the burner brick. The best combustion effect of the regenerative burner can be ensured, the purpose of quickly heating steel billets can be achieved, the requirements for the tapping temperature and temperature difference of the plate blanks in a steel rolling process can be met, and the characteristics ...

Method for quickly clearing fruit and vegetable juice by combining ultrahigh pressure and catalysis by biological enzyme

The invention discloses a method for quickly clearing fruit and vegetable juice by combining an ultrahigh pressure and catalysis by a biological enzyme, which belongs to the field of food processing. In the invention, by taking full advantage of the characteristic of activation of biological enzyme by ultrahigh pressure, fruit and vegetable juice to be cleared and the biological enzyme are placed in an ultrahigh pressure processing kettle, a high pressure of 300MPa is applied at 25 to 45 DEG C to activate the biological enzyme, and thus, the catalysis efficiency of the biological enzyme is improved and the time for clearing the fruit and vegetable juice under the catalytic action of the biological enzyme or the consumption of the biological enzyme is reduced. When the technique is used for clearing juice of fruits and vegetables such as strawberries, raspberries, apples and green peppers, the production cost of clear fruit and vegetable juice is reduced.

Double-heat-storage roller hearth type heating furnace

The utility model relates to a double-heat-storage roller hearth type heating furnace, and belongs to the technical field of steel rolling heating furnaces in the metallurgical industry. The technicalscheme is as follows: the method comprises the following steps: preparing; a plurality of uniformly arranged regenerative burners are respectively arranged on the side A and the side B of the rollerhearth furnace body (1); the heat accumulating type burner comprises an air heat accumulating box (17) and a coal gas heat accumulating box (11). A burner partition plate (14) is arranged between theair heat storage tank (17) and the coal gas heat storage tank (11); a burner block (21) is arranged at one end of the air heat storage tank (17) and one end of the coal gas heat storage tank (11), a premixing cavity is formed in the burner block (21), and a coal gas ignition nozzle (12), an air ignition nozzle (15), a coal gas combustion nozzle (13) and an air combustion nozzle (16) are arranged in the ...

Method for on-line clearing precipitated coals blocked in honeycomb holes of heat accumulator

The invention relates to a method for on-line clearing precipitated coals blocked in honeycomb holes of a heat accumulator. The method belongs to the technical field of industrial heating furnace heat accumulator maintenance methods, and is used for on-line clearing precipitated coals which cause blockage of the honeycomb holes of the heat accumulator. According to the method, an air heat accumulator and a coal gas heat accumulator are subjected to thermal insulation and insulated and burning after discharging smoke and storing heat until reaching the temperature of 200-230 DEG C, and the precipitated coals blocked in the heat accumulator are cleared away. According to the method, only one pair of heat accumulation nozzles needs to be stopped when the precipitated colas are cleared from the heat accumulation nozzles, the furnace box temperature cannot be affected, and the original equipment has no need to be improved. With the method, the precipitated coals blocked in the honeycomb holes ...

Drilling anti-sticking method and device based on mine powder discharge image recognition and medium

The embodiment of the invention discloses a drilling anti-jamming method and device based on mine powder discharge image recognition and a medium. Belongs to the technical field of image data processing. The problem that in the prior art, construction efficiency is reduced due to drilling jamming is solved. The method comprises the following steps: acquiring a powder discharge image uploaded by a camera device group arranged on the anti-sticking device; performing grid division on the pulverized coal discharge image, and determining pulverized coal digital information corresponding to the pulverized coal discharge image based on the quantity of pulverized coal in each grid; based on the pulverized coal digital information, target images which do not meet a preset similarity condition are removed to obtain a second target image set; carrying out the edge description of the pulverized coal discharge images in the second target image set through a coarse registration HOG feature detection ...

Heat accumulating type heating furnace three-way change valve of improved sealing structure

The invention provides a heat accumulating type heating furnace three-way change valve of an improved sealing structure, and belongs to the technical field of heat accumulating type heating furnace equipment. The heat accumulating type heating furnace three-way change valve of the improved sealing structure is used for prolonging the service life of a sealing ring, and the sealing ring can be replaced more conveniently. According to the technical scheme, the sealing ring between an upper valve plate and a lower valve stand is arranged on the upper valve plate, and a connecting rod is arranged between an air cylinder and the upper valve plate. After the structure is used, the working temperature of the sealing ring is lowered, the service life of the sealing ring is prolonged, and operation of the heat accumulating type heating furnace three-way change valve is made reliable. Meanwhile, when the damaged sealing ring is replaced, only the old vale plate needs to be replaced, a new valve plate ...

Application of Knickkopf gene dsRNA in pest control

The invention provides an application of Knickkopf gene dsRNA in pest control, and in particularly relates to a migratory locust Knickkopf gene. A nucleotide sequence of the migratory locust Knickkopf gene is SEQ ID NO: 1; and based on SEQ ID NO: 1, gene segments and corresponding dsRNA are designed and synthesized. After the designed and synthesized dsRNA is injected into bodies of migratory locust, difficult molting of migratory locust can be caused to result in death of migratory locust; and the death rate can reach 76%. The Knickkopf gene obtained by screening, provided by the invention, can be taken as a molecular target for pest control, and can be used for providing a new path for effective control of pests.

Serum type 4 fowl adenovirus ELISA antibody detection kit

The invention discloses a serum type 4 fowl adenovirus ELISA antibody detection kit, and belongs to the technical field of biology. According to the invention, a Fiber-2 antigen protein is used as a target, and a Knob region is repeatedly expressed in series to prepare a recombinant antigen protein. The kit prepared from the recombinant antigen protein provided by the invention is high in sensitivity and strong in specificity, and the maximum dilution multiple of positive serum is 12800 times; when the ELISA antibody detection kit provided by the invention is used for detecting different serotypes of fowl adenoviruses except for serotype 4 fowl adenoviruses, the S/P value is about 0.2, and the specificity is obviously better than that of a commercially available kit (the S/P value is about 0.4). In the components of the kit, an antigen for coating an ELISA plate is recombinant FAdV-4-KN protein expressed by using Sf9 cells, the antigen purity is high, the binding capacity with a virus antibody ...

Heat accumulative roller bottom heating furnace

The invention relates to a heat accumulative roller bottom heating furnace, which belongs to the technical field of steel rolling equipment. Through the application of the heat accumulative combustion technology, the heating quality of steel blanks is improved, and the coal gas consumption is reduced. The invention has the technical scheme that the heat accumulative roller bottom heating furnace comprises a furnace body, a furnace hearth and heat accumulative burner nozzles, wherein the heat accumulative roller bottom heating furnace comprises a plurality of heat accumulative burner nozzles which are uniformly distributed on the upper layers of both sides of the furnace in the furnace length direction, coal gas spray openings and air spray openings are arranged in the heat accumulative burner nozzles, the coal gas spray openings are distributed in the center of the heat accumulative burner nozzles, the air spray openings are uniformly distributed in the peripheral direction of the coal ...

Method for producing proline aminopeptidase by culturing recombinant escherichia coli in high density

The invention discloses a method for producing proline aminopeptidase by culturing recombinant escherichia coli in high density, and belongs to the field of microbial fermentation. Proline aminopeptidase is produced by fermenting escherichia coli, namely recombinant bacteria, by utilizing fermentation mediums and a fermenting method, and the bacteria density (OD600) and the recombinant protein expression quantity are respectively 142 and 211U/mg.

Liquid heater with tea washing cup

The liquid heater comprises a kettle body assembly, a water spraying rod assembly and a filter screen assembly, the detachable tea washing cup is arranged in a kettle opening in the top of the kettle body assembly, the filter screen assembly is detachably arranged in the tea washing cup, the water spraying rod assembly is arranged in the kettle body assembly, and the water spraying rod assembly is arranged in the kettle body assembly. And the upper end of the water spraying rod assembly penetrates through the tea washing cup and then extends into the filter screen assembly, so that the use convenience of a product can be improved, and the use experience of a user can be improved.

Long-life steel billet conveying roller way for heating furnace

The invention discloses a long-life steel billet conveying roller way for a heating furnace and belongs to the technical field of heating furnace equipment, the long-life steel billet conveying roller way is used for conveying steel billets in the heating furnace. The technical scheme is as follows: the steel billet conveying roller way is not provided with a water-cooling facility, wherein a roller body adopts a circular-tube shaped thin-wall structure; a plurality of ring-shaped convex roller rings are arranged on the outer wall of the circumference of the roller body, and the plurality of roller rings are uniformly distributed on the roller body along the roller body axial direction. The roller body and the roller neck of the long-life steel billet conveying roller way disclosed by the invention are cast by high-temperature alloy, the furnace roller is not equipped with a water-cooling structure, a furnace roller surface temperature and a furnace chamber temperature are the same, and ...

Normal-temperature fast curing epoxy resin crack pouring agent for roads and bridges

A normal-temperature fast curing epoxy resin crack pouring agent for roads and bridges comprises a component A and a component B, the component A is mainly composed of an epoxy resin, an accelerant, a modified flexibilizer, an epoxy silane coupling agent, a reactive diluent and a coloring agent, the modified flexibilizer is a polyurethane prepolymer of toluene diisocyanate modified hydroxy-terminated polybutadiene liquid rubber, the accelerant is tetrabutylammonium iodide, the epoxy silane coupling agent is KH-560 or KH-530, the component B is mainly composed of a curing agent, the accelerant and an antifoaming agent, and the curing agent is a mixture containing polyetheramine and phenolic aldehyde amine with a mass ratio of 75:25-94:6. A mass ratio of the component A to the component B is 100:80-100:100. The crack pouring agent has the advantages of high bonding strength, good toughness, low viscosity, good wettability, normal temperature curing, fast curing speed and the like. The crack ...

Process for heating continuous casting slabs by regenerative roller-hearth type heating furnace

The invention relates to a process for heating continuous casting slabs by a regenerative roller-hearth type heating furnace and belongs to the technical field of process for heating slabs by a heat-accumulation heating furnace. The process is used for rapidly heating continuous casting thin slabs. According to the technical scheme, the various functional segments of the roller-hearth type heating furnace are divided into a first heating section, a second heating section, a third heating section, a slab traversing section and a thermal-insulation section, a regenerative combustion technology is adopted in each section, and the temperature of the first heating section as a main heating section is raised to 1230 DEG C, the temperature of the second heating section as an auxiliary heating section is raised to 1210 DEG C, the temperatures of the other sections are also raised to 1190 DEG C, the soaking process is completed, and the final tapping temperature is 1180+/-5 DEG C. According to the ...

Regenerative hot rolled strip heating furnace and slab heating process

The invention provides a regenerative hot rolled strip heating furnace and a slab heating process, belonging to the technical field of regenerative heating furnace equipment and slab heating processes. The heating furnace and the slab heating process are used for heating slabs. According to the technical scheme, the heating furnace is characterized in that a hearth of the heating furnace is provided with a first heating zone, a second heating zone and a soaking zone; each heating zone adopts an air-gas double regenerative burner structure; the hearth temperature of the first heating zone is 1150-1200 DEG C and the heating load of the first heating zone is 40-50% of the rated load; the hearth temperature of the second heating zone is maintained at 1250-1280 DEG C and the heating load of the second heating zone is 40-50% of the rated load; the furnace temperature of the soaking zone is 1230-1250 DEG C and the heating load of the soaking zone is 15-20% of the rated load. By adopting the heating ...

Power lithium battery pack balancing and managing system based on Map model

The invention discloses a power lithium battery pack balancing and managing system based on a Map model, which comprises: a central processor, a charging module, a balancing module, a data collecting module, an electric quantity calculating module, a data display module and a storage communication module, wherein the central processor is respectively connected with the charging module, the balancing module, the data collecting module, the electric quantity calculating module, the data display module and the storage communication module, and the data collecting module is respectively connected with a voltage collecting module, a current collecting module and a temperature collecting module; the invention has the advantages of balancing the charging of batteries better, realizing the protection on a single battery and prolonging the service life of the battery, and reducing energy consumption. In addition, by using a service life accelerating factor and a cycle life forecast, the invention ...

Porcine parvovirus genetic engineering epitope vaccine and preparation method thereof

The invention discloses a porcine parvovirus genetic engineering epitope vaccine and a preparation method thereof, and belongs to the field of veterinary biological products. The preparation method of the vaccine provided by the invention comprises the following steps: by taking a hepatitis B virus core antigen protein as a carrier, inserting a fusion epitope constructed by a porcine parvovirus VP2 protein Th epitope, a CTL epitope and a B cell epitope between 79th amino acid and 80th amino acid of a hepatitis B virus core protein (HBc) gene; expression is constructed by using an insect cell-baculovirus expression system, and the recombinant virus efficiently expresses antigen protein in insect cell HF; after extraction, purification and BEI inactivation, adjuvants are added for emulsification, and the vaccine is prepared. The preparation method is simple, can prepare a large amount of antigen protein, is short in time consumption and high in expression quantity, greatly reduces the production ...

Dry spinning process of making bright polyacrylic fibre

Virus-like particle vaccine for preventing avian reovirus and preparation method thereof

The invention discloses a virus-like particle vaccine for preventing avian reovirus and a preparation method of the virus-like particle vaccine, and belongs to the field of veterinary biological products. The virus-like particle vaccine for preventing the avian reovirus, provided by the invention, contains fusion protein of which the coding gene sequence is shown as SEQ ID NO.2, and the fusion protein can be self-assembled to form virus-like particles. The invention further discloses a preparation method and application of the virus-like particle vaccine for preventing the avian reovirus. The virus-like particle vaccine of the avian reovirus is good in immune effect and small in immune dosage, can generate protection within 14 days after immunization, and effectively prevents diseases caused by the avian reovirus. The preparation method disclosed by the invention is simple, can be used for preparing the antigen protein of the avian reovirus disease on a large scale, is short in time consumption ...