12-05-2023 дата публикации
Номер: CN116106276A
Принадлежит:
The invention belongs to the technical field of detection of antibiotic residues in agricultural and sideline products, and particularly relates to a quantitative detection method of ampicillin in milk based on up-conversion fluorescence internal filtration. Based on fluorescence internal filtration, a solid-phase capture probe and artificial nano-enzyme are introduced, and the method for quantitatively detecting ampicillin in milk based on up-conversion fluorescence internal filtration is constructed. When the ampicillin exists in the system, the solid-phase capture probe captures the ampicillin, the aptamer functionalized platinum nano-particles are specifically adsorbed to the surface of the solid-phase capture probe to catalyze TMB oxidation, and a product and up-conversion generate a fluorescence inner filter effect and fluorescence quenching; along with the increase of the concentration of the ampicillin, the more the platinum nanoparticles loaded on the surface of the solid-phase ...
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