Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in escherichia coli or the mutant thereof with any of the following regulating elements: artificial regulating element M 1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.

Recombinant microorganism for generating terpenoid and construction method thereof

The invention discloses a recombinant microorganism for generating terpenoid and a construction method of the microorganism. A recombinant stain 1 is provided by the invention to improve the activity of alpha-ketoglutarate dehydrogenase in colon bacillus or a mutant strain of the colon bacillus to obtain a recombinant strain. The method for improving the activity of the ketoglutarate dehydrogenase in the colon bacillus or the mutant strain of the colon bacillus comprises the following step of substituting any one of the following regulatory elements for the original regulatory element of a ketoglutarate dehydrogenase gene suc AB in the colon bacillus or the mutant strain of the colon bacillus: artificial regulatory elements M1-46, M1-37, and M1-93. Experiments prove that, according to the construction method disclosed by the invention, a plurality of recombinant strains are constructed, and NADPH (Nicotinamide Adenine Dinucleotide Phosphate) and ATP (Adenosine Triphosphate) synthesis capacity ...

RECOMBINANT MICROORGANISM FOR PREPARING TERPENOID AND METHOD FOR CONSTRUCTING RECOMBINANT MICROORGANISM

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase in or the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase in or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in or the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved. 1E. coli. A recombinant strain 1 , which is constructed by a method comprising: improving enzymatic activity of α-ketoglutarate dehydrogenase of or a mutant thereof , to obtain the recombinant strain 1.2. The recombinant strain 1 according to claim 1 , wherein:{'i': E. coli', 'E. coli, 'the enzymatic activity of α-ketoglutarate dehydrogenase of or a mutant thereof is improved by replacing an original regulatory part of an α-ketoglutarate dehydrogenase gene, sucAB, of or a mutant thereof with any one of the following artificial regulatory parts M1-46, M1-37 or M1-93; whereinartificial regulatory part M1-46 has a nucleotide sequence of SEQ ID NO: 14;artificial regulatory part M1-37 has a nucleotide sequence of SEQ ID NO: 10;artificial regulatory part M1-93 has a nucleotide sequence of SEQ ID NO: 11; andthe original regulatory part of α-ketoglutarate dehydrogenase gene is SEQ ID NO: 15.4. A recombinant strain 1-1-A or a recombinant strain 1-1-B claim 1 , wherein:{'claim-ref': [{'@idref': 'CLM-00003', ' ...

Recombinant microorganism for preparing terpenoid and method for constructing recombinant microorganism