CELL CULTURE SYSTEMS

An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels. 1. A cell culture system comprising:at least one cell culture vessel configured to culture cells using a plurality of parallel cell culture surfaces, wherein the at least one cell culture vessel comprises at least one port configured to allow material to flow into and out of the at least one cell culture vessel;manipulation apparatus configured to rotate the at least one cell culture vessel about a first rotation axis and about a second rotation axis, wherein the first rotation axis is perpendicular to the second rotation axis, wherein each of the first rotation axis and the second rotation axis are parallel a ground surface;pumping apparatus fluidly coupled to the at least one port of the at least one cell culture vessel and configured to pump material into and out of the at least one cell culture vessel through the at least one port;monitoring apparatus configured to monitor one or more parameters of the at least one cell culture vessel, the manipulation apparatus, and the pumping apparatus; andcontrol apparatus operably coupled to the manipulation apparatus, the pumping apparatus, and the monitoring apparatus, wherein the control apparatus is configured to coordinate movement of the at least one cell culture vessel using the manipulation apparatus with the pumping of material into and out of the at least one culture vessel using the pumping apparatus.2. The system of claim 1 , wherein the control apparatus is further configured to:move, using the manipulation apparatus, the at least one cell culture vessel into at least one fill ...

VESSEL FOR GROWTH OF BIOLOGICAL ENTITIES

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5× the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel. 1. A vessel for culturing cells comprising:a vessel main body defined by a bottom having rounded edges that is integral with a conically-shaped sidewall that tapers inward to a top surface; andan integral elongate annular neck extending upwardly from the top surface to a vessel opening, whereinthe sidewall is tapered inward with an inclination angle of 4-10°.2. The vessel according to claim 1 , wherein the top surface is substantially horizontal.3. The vessel according to claim 1 , wherein the vessel main body has a volume of at least 5 liters.4. The vessel according to claim 1 , wherein the vessel comprises a unitary part.5. The vessel according to claim 1 , wherein the vessel has a hydrophilic inner surface.6. The vessel according to claim 1 , wherein the neck is joined to the vessel main body via a tapered transition area.7. The vessel according to claim 1 , wherein the neck diameter is less than a diameter of the vessel opening.8. The vessel according to claim 1 , wherein the neck diameter is at least 30% of a maximum vessel diameter.9. The vessel according to claim 1 , further comprising two or more interior baffles configured to disrupt a liquid vortex of a liquid media contained within the vessel during agitation of the liquid media.10. The vessel according to claim 9 , where the baffles are raised with respect to an inner surface of the vessel.11. The ...

CELL CULTURE SYSTEM WITH MANIFOLD

A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves. 116-. (canceled)17. A cell culture apparatus having a cascading manifold , comprising: each cell culture chamber comprising a top surface, a bottom surface configured to culture cells comprising gas permeable, liquid impermeable film, and a plurality of cell culture chamber openings; and,', 'a plurality of spacer layers between the plurality of cell culture chambers, adjacent to the gas permeable, liquid impermeable film and exterior to each of the cell culture chambers; and,, 'a plurality of cell culture units, each cell culture unit comprising a plurality of stacked cell culture chambers;'}a cascading manifold comprising an inlet, an overflow outlet and a plurality of openings in fluid communication with each of the plurality of cell culture chamber openings of the cell culture chambers of a cell culture unit;wherein fluid, introduced into a cascading manifold of a first cell culture unit through its inlet, flows into each cell culture chamber of the first cell culture unit through the plurality of cell culture chamber openings until each cell culture chamber of the cell culture unit is filled with fluid and then, once each cell culture chamber of the cell culture unit is filled, the cascading manifold fills with fluid and fluid cascades out of the cascading manifold through its overflow outlet;wherein the overflow outlet of the cascading manifold of a first cell culture unit is coupled to the inlet of a second manifold of a second cell culture unit; ...

Energy conversion system

An energy conversion system comprising: a riser conduit comprising a first liquid; a down-comer comprising a second liquid, the down-comer in fluid communication with the riser conduit, the second liquid comprising first liquid and finely divided material in suspension such that the second liquid has a higher specific gravity than the first liquid, the down-comer in fluid communication with a first tank and a second tank; a converter device arranged to convert energy of the first liquid into energy for output from the energy conversion system, and to discharge the first liquid thereafter; and a recirculator arranged to recirculate third liquid to maintain the finely divided material in suspension, the third liquid comprising second liquid and further finely divided material in suspension. The recirculator is arranged to discharge the third liquid to mix with the first liquid from the converter device to form the second liquid. The first tank and the second tank are arranged between the converter device and the down-comer, to receive the first liquid discharged from the converter and to supply the first liquid to the down-comer. Supply of first liquid to the first and second tanks is in use regulated to maintain the height of liquid in the first and second tanks below a predetermined threshold.

PIPETTE TIPS WITH ENHANCED ATTRIBUTES AND METHODS FOR MANUFACTURING

A pipette tip includes a proximal end having an aperture and a proximal body region having a first internal cavity that receives a pipette member, and a distal end having an aperture and a distal body region having a second internal cavity that in operation receives and discharges a liquid aspirated by the pipette member, where the ratio of the proximal half weight of the pipette tip to the distal half weight of the pipette tip normalized to the total weight of the pipette tip has a value, defined as a thinning constant, wherein the pipette tip has a wall thickness of less than 0.022 inches over the distal region. 117-. (canceled)19. The pipette tip of wherein the T is equal to or greater than 18.20. The pipette tip of wherein the pipette tip length is between 1.75 inches and 2.25 inches.21. The pipette tip of wherein the distal half of the pipette tip length has a wall having a thickness of less than 0.022 inches.22. The pipette tip of claim 18 , wherein the wall thickness of the distal half of the pipette tip length does not exceed 0.029 inches.23. The pipette tip of claim 18 , wherein the distal half of the pipette tip length has a wall having a thickness of less than 0.009 inches.24. The pipette tip of claim 18 , wherein the distal half of the pipette tip length has a wall having a thickness of less than 0.008 inches.25. The pipette tip of claim 18 , wherein the proximal half of the pipette tip length has a wall having a thickness of between 0.02 inches and 0.03 inches.26. The pipette tip of wherein the pipette tip comprises a single polymer or a blend of polymers.27. The pipette tip of claim 26 , wherein the average crystal size of the polymer or polymers is less than 6 microns.281. The pipette tip of claim claim 26 , wherein the proximal half of the pipette tip length further comprises a plurality of radial fins.291. The pipette tip of claim having a tip straightness differential length of less than 0.001 inches.30. The pipette tip of claim 18 , wherein Wt is ...

CELL CULTIVATING FLASK

A flask for the culturing of cells is disclosed. The cell culture chamber is defined by top and bottom walls connected by side and end walls, one end wall shaped such that media can drain to a bottommost spot thereby allowing for the complete removal of media via a vertically oriented pipette inserted into the interior of the flask body through an open neck. 1. A flask for the growth of cells comprising:a flask body serving as a cell culture chamber defined by a bottom wall having a rigid surface and a top wall, the bottom wall and top wall connected by side walls and first and second walls,a neck portion to the body open at one end and connected to the body at the other end providing access to the flask body;wherein an interior portion of said first end wall is sloped such that when the flask is situated with the open end of the neck facing upwards, the lowest-most point of the interior portion of the first end wall is located in vertical alignment with the open end of the flask2. The flask according to claim 1 , wherein the neck opening has a diameter of between 25 and 35 mm.3. The flask according to claim 1 , further comprising a cap that fittingly engages the neck in order to seal the contents of the flask.4. The flask according to wherein said bottom portion further comprises a substantially flat region defined by a substantially rectangular portion and an arched portion.5. The flask according to wherein the bottom wall further comprises an included ramp connecting the neck to the substantially flat region.6. The flask of according to wherein said second end wall comprises diverging sloping portions which extend from a central portion of the side walls7. The flask according to further comprising feet affixed to an external portion of the first side wall.8. The flask according to wherein the interior portion of the first end wall comprises two sections meeting at a focal point and creating an angle claim 1 , the angle being between 150 and 175 degrees.9. The ...

Cell culture systems

An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels.

Energy conversion system

An energy conversion system comprising: a riser conduit comprising a first liquid; a down-comer comprising a second liquid, the down-comer in fluid communication with the riser conduit, the second liquid comprising first liquid and finely divided material in suspension such that the second liquid has a higher specific gravity than the first liquid, the down-comer in fluid communication with a first tank and a second tank; a converter device arranged to convert energy of the first liquid into energy for output from the energy conversion system, and to discharge the first liquid thereafter; and a recirculator arranged to recirculate third liquid to maintain the finely divided material in suspension, the third liquid comprising second liquid and further finely divided material in suspension. The recirculator is arranged to discharge the third liquid to mix with the first liquid from the converter device to form the second liquid. The first tank and the second tank are arranged between the converter device and the down-comer, to receive the first liquid discharged from the converter and to supply the first liquid to the down-comer. Supply of first liquid to the first and second tanks is in use regulated to maintain the height of liquid in the first and second tanks below a predetermined threshold.

Cell Visualization System for Multi-Layer Cell Culture Device

The present invention relates generally to a multi-layer system for containing cells in culture, and a system for visualizing cells cultured in the multi-layered system. More specifically, the present invention relates to a multi-layer cell culture device having a specialized bottom plate and a microscope adaptor which can accommodate a microscope to allow microscopic visualization of cells cultured in the device.

Inexhaustible prime mover

Inexhaustible prime mover

Apparatus for providing motive power comprises first and second communicating columns (10, 14) of liquid, the liquid in the second column being substantially the same as in the first column but containing a finely divided material to increase its specific gravity relative to the liquid in the first column, means (28) for maintaining the finely divided material in suspension in the second column, whereby liquid height in the respective columns is different, so that liquid from the first column overflows into the second column, means for converting energy from resultant movement of liquid into motive power and means (26) for returning the liquid to the first column.

Vessel for growth of biological entities

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5x the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Vessel for growth of biological entities

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5x the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.