Arrays of nucleic acid probes for analyzing biotransformation genes

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different ...

Arrays of nucleic acid probes on biological chips

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.

Amplification of nucleic acids with electronic detection

The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.

Integrated portable biological detection system

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Integrated portable biological detection system

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Arrays of nucleic acid probes for analyzing biotransformation genes

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different ...

Devices and methods for biochip multiplexing

The invention is directed to devices that allow for simultaneous multiple biochip analysis. In particular, the devices are configured to hold multiple cartridges comprising biochips comprising arrays such as nucleic acid arrays, and allow for high throughput analysis of samples.

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

Multi-chambered analysis device

A device includes an inlet for receipt of a sample. A first chamber is coupled to the inlet and includes at least one affinity region. A second chamber is disposed adjacent to the first chamber. The first chamber and the second chamber share a common intermediate member, the intermediate member having at least one via formed in the common intermediate member. The second chamber includes an assay chip comprising an array of addressable electrodes. An outlet is coupled to the second chamber. The device may be used to selectively amplify and elute nucleic acids for subsequent detection and analysis.

ARRAYS OF NUCLEIC ACID PROBES FOR DETECTING CYSTIC FIBROSIS

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene. 130-. (canceled)31. A device for detecting at least one variation in the splicing of a gene comprisingan array of nucleic acid probes immobilized on a solid support, the array comprising at least two sets of probes of between 3 and 100 nucleotides in length,wherein said array comprises at least a first and a second probe,wherein said first probe comprises a first sequence that is complementary to an exon or an intron of a gene, and wherein said sequence corresponds to at least one region of variation corresponding to a splice sequence, andwherein said second probe comprises a second sequence that is complementary to an exon-intron boundary of said gene, and wherein said second sequence corresponds to at least one region of variation corresponding to a splice sequence,said device allowing, when hybridized with a target sequence, detection of the presence or absence of said at least one variation in the splicing of a gene.32. The device of claim 31 , wherein said probe sequences are publicly available.33. The device of claim 31 , wherein the probes are immobilized on a chip.34. The device of claim 31 , wherein said probes are oligodeoxyribonucleotides or oligoribonucleotides.35. The device of claim 31 , wherein said probes comprise sequences of between 3 and 50 nucleotides.36. The device of claim 31 , wherein said first and second probes exhibit complementarity to reference sequences comprising mutations or polymorphisms associated with phenotypic changes having clinical significance in human patients.37. The device of claim 36 , wherein said first and second probes exhibit complementarity to reference sequences comprising mutations or polymorphisms associated with cancer.38. A method of producing a device comprising an array of nucleic acid probes immobilized on a solid support claim 36 , the array comprising at least two sets of probes of ...

Arrays of Nucleic Acid Probes for Analyzing Biotransformation Genes

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets. 1. An array of nucleic acid probes immobilized on a solid support , the array comprising at least two sets of probes ,(1) a first probe set comprising a plurality of probes, each probe comprising a segment of at least six nucleotides exactly complementary to a subsequence of a reference sequence, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence,(2) a second probe set comprising a corresponding probe for each probe in the first probe set, the corresponding probe in the second probe set being identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least six nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the two corresponding probes from the first and ...

Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces

Substrates with surfaces comprising compounds with thiol functional groups protected with a photoremovable protecting group can be used to construct arrays of immobilized anti-ligands, such as oligonucleotide probes or other biological polymers. The arrays can be used in assays to detect the presence of complementary nucleic acids in a sample. Spatially addressed irradiation of predefined regions on the surface permits immobilization of oligonucleotides and other biological polymers at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allow formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix.

Arrays of nucleic acid probes on biological chips

The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.

Arrays of nucleic acid probes on biological chips

DNA chips containing arrays of oligonucleotide probes can be used to determine whether a target nucleic acid has a nucleotide sequence identical to or different from a specific reference sequence. The array of probes comprises probes exactly complementary to the reference sequence, as well as probes that differ by one or more bases from the exactly complementary probes.

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

Pulse radar

A layered cementitious composition which time releases permanganate ion

A layered composition which includes a hardened core comprising at least about 50 weight percent permang-anate which composition releases permanganate ion into an aqueous media over time is described. A method of making and using the composition of the invention also are described.

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

Process for labeling nucleic acids using psoralen derivatives

A labelling reagent of the formula: is prepared where A is an alkylating intercalation moiety, B is a divalent organic spacer arm moiety with a straight chain of at least two carbon atoms, and L is a monovalent label moiety capable of producing a detectable signal, e.g., a signal detectable by spectroscopic, photochemical, chemical, immunochemical or biochemical means. Preferably A is a 4'-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted 4,5', 8-trimethylpsoralen moiety. This reagent may be used to label nucleic acids, preferably DNA, by intercalating the alkylating intercalation moiety of the reagent into an at least partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. This reagent may also be used in chromosome banding to label specific regions of chromosomes and thereby differentiate them.

System including functionally separated regions in electrophoretic system

Methods, apparatus, and applications for use of a stacked, reconfigurable system for electrophoretic transport are provided. In one embodiment, a system having a first chamber including at least a bottom support and an intermediate support, and a second chamber, said second chamber including a bottom support and a top member, the first and second chambers being coupled through a via. Electrophoretic, and optional electro-osmotic and thermal, transport is effected. In another aspect of this invention, three or more chambers are coupled by an electrophoretic buss. The electrophoretic buss includes driving electrodes and is adapted to receive fluid containing materials for transport. The chambers are coupled to the electrophoretic buss and serve as a tap from the buss for delivery of charged materials. In one embodiment, certain functions are performed in different chambers. For example, the first chamber may receive the sample and perform sample processing functions, the second chamber may perform amplification procedures, yet a third chamber may perform hybridization or other assays, and yet another chamber may perform immunoassays. By separating various functions to different chambers, speed and sensitivity may be improved. In yet another aspect of this invention, analysis from a earlier stage may be utilized in a subsequent stage to reconfigure the system for optimum use. In one application, analysis at a first level is utilized to determine an action at a second level, such as the synthesis of a compound. The synthesized compound in response to a biohazard may comprise vaccine or antidote.

Composite photosensitive screens

Amplification of nucleic acids with electronic detection

The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.

Radar receiver

IMPROVED RADAR RECEIVER Abstract of the Disclosure An improved radar receiver is shown to include a plurality of intermediate frequency and video frequency amplifiers, each one of such amplifiers being arranged to process received signals within a different range of amplitudes so that the overall dynamic range of such receiver may be equivalent to the dynamic range of a conventional radar receiver with automatic gain control.

Apparatus for supersonic examination of bodies

DNA detection method

Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Electro-optical devices for detecting images of invisible radiations using interaction of light beams

This invention relates to devices for detecting images of invisible radiations and reproducing them as visible images. These devices are provided with detecting means which upon irradiation by a beam of invisible radiation carrying the image of the examined body exhibit various ''''optical'''' changes. These changes are analyzed in a scanning mode point after point by a monochromatic light beam which after recombination with nonmodulated part of said monochromatic light beam provides readout information about said invisible image. This scanning readout is characterized by means providing the same angle of incidence of light beams on all successive points of said detecting means. The above readout information is received and subsequently converted by photoelectric means into an image. In another embodiment of this invention instead of scanning readout means are provided to produce simultaneously a plurality of combined monochromatic light beams for simultaneous readout of said optical changes. It is also characteristic for this embodiment that photoelectric means receive said plural combined light beams simultaneously.

Electronic system sensitive to invisible images

Endoscopic device

Aircraft auxiliary gas turbine engine and method for operating

A non-aircraft-propelling auxiliary gas turbine engine (10) installable in an aircraft (12) having a cabin (16) adapted to be pressurized. The auxiliary gas turbine engine includes an auxiliary-gas-turbine-engine compressor (18) having an inlet (20), wherein the inlet is adapted to receive pressurized air (22) from the cabin.

FIELDS OF NUCLEIC ACID PROBE ON ORGANIC CHIPS

Opto-electric screens provided with light-conducting members

This invention relates to novel composite photoelectric screens characterized by the construction in which the supporting member for the photoelectric means is radiation transparent and is made of an array of said radiation-conducting fibers. The lightconducting fibers operate by the internal reflection and are arranged spatially in such a manner that they can transfer the radiation image across said support without any appreciable loss of resolution, contrast and brightness.

Reliability check circuits

DNA detection method

Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Servo valve for well-logging telemetry

Vacuum tubes provided with light conducting members and with a screen of a high photoelectric sensitivity

DNA detection system

Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

Tunable optical fiber buildout

A tunable optical fiber buildout for coupling a first optical fiber cable to a second optical fiber cable is disclosed. The buildout is comprised of a buildout base formed about a longitudinal axis and a buildout cap constructed and arranged to be received within the base. The base is constructed and arranged to be received within the cap in one of a plurality of rotational positions about the longitudinal axis of the base so that the cap can be used to selectively optimize an optical signal passed through at least one of the first and the second optical fiber cables, respectively, as the cap is positioned in one of the plurality of rotational positions about the axis of the base.

Cathode-ray tubes of television type for x-rays protection

The invention relates to novel cathode-ray/vacuum tubes for color television. It was found that the present color television tubes emit considerable amount of X-radiation. The novel tubes described below are characterized by the X-ray absorbing faceplate which prevents the escape of such X-radiation or at least reduces the amount of said escaping X-radiation to the level which is safe for the public. These tubes have a construction in which their light transparent endwall on which the fluorescent screen is mounted has the X-ray absorbing power to accomplish this objective, which means to reduce the transmission of X-ray through said endwall to the amount smaller than 0.04 mr./hr. in addition the faceplate of said tubes is provided with a light partially absorbing material which means light filtering material to improve the contrast of images produced by said tubes.

Analysis of genetic polymorphisms and gene copy number

The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

Well logging system arranged for stable,high-sensitivity reception of propagating electromagnetic waves

A layered cementitious composition which time releases permanganate ion

A layered composition which includes a hardened core comprising at least about 50 weight percent permang-anate which composition releases permanganate ion into an aqueous media over time is described. A method of making and using the composition of the invention also are described.

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge ( 10 ) including a microfabricated silicon chip ( 12 ) on a printed circuit board ( 14 ) and a flow cell ( 16 ) mounted to the chip ( 12 ) to form a flow chamber. The cartridge ( 10 ) also includes output pins ( 22 ) for electronically connecting the cartridge ( 10 ) to an electronic controller. The chip ( 12 ) includes a plurality of circular microelectrodes ( 24 ) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

Auxiliary power unit assembly

An embodiment of the technology described herein is an auxiliary power unit assembly. The auxiliary power unit assembly includes an auxiliary power unit being installable in an aircraft having a cabin, a duct connecting the cabin and the auxiliary power unit, and an airflow management feature in the duct.

Process for labeling nucleic acids and hybridization probes

Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula: [A--[B--L where A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties. Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moeity and L is biotin.

Sleeve carrier for fiber optic adapters

Syringe

Radiation transporting devices provided with hollow members mounted in an array

Universal and modular interchangeable attachment for optical fiber

Method of operating pulse radar

A method, and exemplary apparatus for practicing the method, is shown for operating a pulse radar to allow the radar ambiguity function of such radar to be modified by controlling the range and Doppler ambiguities due to the pulse waveform of such radar.

Improvements to address making machines

Dna detection system

36. DNA DETECTION SYSTEM ABSTRACT OF THE DISCLOSURE Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.

A gas turbine auxiliary power unit (APU) in an aircraft being supercharged by two different compressed gas streams

An auxiliary power unit (APU) 10 of an aircraft comprises a gas turbine engine assembly 12 having the intake 22 of its compressor 20 fluidly connected to the outlet 28 of a mixing damper 14. The mixing damper comprises two inlets 26, 28 receiving a first gas stream 30 and a different second gas stream 32, both gas streams having been compressed by at least one main propulsion gas turbine engine 18. The first gas streams may be bleed air 38 direct from the fan or compressor of a main gas turbine 18, or indirectly in the form of bleed air 50 which has been circulated through the cabin 52, or waste gas of an onboard oxygen generation system, onboard inert gas generation system, or onboard air-cooling environmental control system processing bleed air. The mixing damper may be a plenum, a turbo-expander, a turbo-compressor, or an ejector. Both gas streams may originate from the same gas turbine engine. The APU is thereby supercharged.

Aircraft auxiliary gas turbine engine and method for operating

A non-aircraft-propelling auxiliary gas turbine engine (10) installable in an aircraft (12) having a cabin (16) adapted to be pressurized. The auxiliary gas turbine engine includes an auxiliary-gas-turbine-engine compressor (18) having an inlet (20), wherein the inlet is adapted to receive pressurized air (22) from the cabin.

System of intensification of x-ray images

Device for intensifying x-ray images

Universal modular optical fiber buildout

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

Printing-machine.

Apparatus for fluoroscopy and radiography

Hapten derivatized capture membrane and diagnostic assays using such membrane

The present invention encompasses a capture membrane comprising a porous filter membrane having a hapten bound directly or indirectly to the membrane wherein complexes formed by specific binding having an anti-hapten bound to a binding member of the specifically binding complex are removed from a solution by the hapten as the solution passes through the membrane. In the preferred embodiment biotin is the hapten and avidin or streptavidin is the anti-hapten.

Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis

The present invention comprises devices and methods for performing channel- less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

Apparatus and methods for active biological sample preparation

Systems and methods for the electronic sample preparation of biological materials utilize the differential charge-to-mass ratio and/or the differential affinity of sample constituents to separation materials for sample preparation. An integrated system is provided for performing some or all of the processes of: receipt of biological materials, cell selection, sample purification, sample concentration, buffer exchange, complexity reduction and/or diagnosis and analysis. In one embodiment, one or more sample chambers adapted to receive a buffer solution are formed adjacent to a spacer region which may include a trap or other affinity material, electrophoretic motion of the materials to be prepared being effected through operation of electrodes. In another aspect of this invention, a transporter or dipstick serves to collect and permit transport of materials, such as nucleic acids, most preferably DNA and/or RNA. In one embodiment, a membrane or trap is held in a frame which is adapted to mate with a channel formed in the spacer region. In another aspect of this invention, an electrophoretic system for biological sample preparation is operated in a manner so as to utilize the differential charge-to-mass ratio so as to control the migration of materials within the solution. In one aspect, bunching of selected materials is achieved by operation of two electrodes in a manner so as to reduce the spatial dispersion of those materials. In another aspect of this invention, a vertically disposed sample preparation unit includes an upper reservoir including and a collection chamber. A sample is preferably pre-prepared and densified, applied to the conductive polymer, electrophoresed so as to move nucleic acids into the conductive polymer and move undesired material away from the conductive polymer. Integrated systems are described in which cell separation, purification, complexity reduction and diagnosis may be performed together. In the preferred embodiment, cell separation ...

Printing-machine.

Apparatus and methods for active biological sample preparation

Aparelhos e métodos para o preparo de amostra biológica ativa

Patente de Invenção: <B>"APARELHOS E MéTODOS PARA O PREPARO DE AMOSTRA BIOLóGICA ATIVA<D>". Sistemas e métodos para a preparação de amostra eletrónica de materiais biológicos utiliza a proporção diferencial de carga para massa e/ou a afinidade diferencial dos constituintes da amostra para a separação de materiais para a preparação de amostra. Um sistema integrado é provido para efetuar alguns ou todos os processos de: recebimento dos materiais biológicos, seleção da célula, purificação da amostra, concentração da amostra, troca do solução compensadora, redução de complexidade e/ou diagnose e análise. Em uma modalidade, uma ou mais câmaras de amostra adaptadas para receber uma solução solução compensadora são formadas adjacentes a uma região do espaçador que pode incluir um coletor ou outro material de afinidade, o movimento eletroforético dos materiais a serem preparados sendo efetuado através da operação de eletrodos. Em um aspecto da presente invenção, um transportador ou dipstick serve para coletar e permitir o transporte de materiais, tais como ácido nucleico, mais preferivelmente DNA E/OU RNA. Em uma modalidade, uma membrana, ou coletor, é mantida em uma estrutura que é adaptada para se encaixar a um canal formado na região do espaçador. Em outro aspecto da presente invenção, um sistema eletroforético para preparação de amostra biológica é operado de modo tal a utilizar a proporção diferencial de carga para massa, de modo a controlar a migração de materiais dentro da solução. Em um aspecto, o agrupamento de materiais selecionados é obtido por meio da operação de dois eletrodos de modo tal a reduzir a dispersão espacial desses materiais. Em outro aspecto da presente invenção, uma unidade de preparação de amostra disposta verticalmene inclui um reservatório superior e uma câmara de coleta. Uma amostra é preferivelmente pré-preparada e densificada, aplicada ao polímero condutor, submetida a eletroforese, de modo a mover os ácidos nucleicos para ...

Complexity reduction system for biological sample preparation

A complexity reduction system for a biological sample preparation comprises a printed circuit board containing vias in which a conductive support material is located to capture probes, a chamber adapted to mate with the circuit board, at least one disposal region for the attraction of undesired materials and at least one electrode.

Integrated device including a DNA, RNA or protein trap for biological sample preparation

Pyrilamine-resin preparations

Addressing machine

Improvements in methods of and apparatus for degassing flexible collapsible bags

Haptenderivatisierte aufnahmemembran und diagnostische tests, die eine solche membran verwenden

Adhesive applying and drying mechanism for sheets

Method for selective isolation of desired charged biological materials from undesired charged materials

Apparecchiatura per incurvare e per temprare lastre di vetro.

Perfezionamento nei sistemi radar

Adressendruckvorrichtung.

Spring-wheel.

Improvements in methods of and apparatus for degassing flexible collapsible bags

Haptenderivatisierte aufnahmemembran und diagnostische tests, die eine solche membran verwenden

Process and apparatus for recovery of solid thermoplastic polymers from solution

Stapling mechanism

Machine for wiring blanks

Apparatus for well logging telemetry

Composition comprising stabilized hydrocracked lube andan antioxidant

Composition comprising stabilized hydrocracked lube andan antioxidant

Vorrichtung zum Einrollen und Einschleifen von Zeitungen, Prospekten und ähnlichen Gegenständen.

Label-affixing machine.

Process and apparatus for recovery of solid thermoplastic polymers from solution

Fcc sulfur oxide acceptor

Anordnung mit einem polarisationsunempfindlichen Phasenschieber

Apparatur for maaling i borehull

Valve for use in well logging telemetry

Well logging telemetry

Valve for use in well logging telemetry

Kanallose trennung von biopartikeln auf einem bioelektronischen chip durch dielektrophoresis

Laminated assembly for active bioelectronic devices

Montagem laminada para dispositivo bioeletrÈnicos ativos

Patente de Invenção: <B>"MONTAGEM LAMINADA PARA DISPOSITIVOS BIOELETRÈNICOS ATIVOS"<D>. Processos de fabricação e dispositivos para a execução de operações biológicas ativas que utilizam laminadas (30, 11). Na concretização preferida, um primeiro suporte plano de amostra (50, 72, 104, 122) inclui pelo menos um furo atravessante de amostra (56, 74, 90), um eletrodo plano (32, 70, 94, 112, 114) é disposto adjacente ao primeiro suporte plano de amostra (50, 72, 104, 122), e inclui uma região atravessante de eletrodo (38, 76), um segundo suporte plano (40, 78, 96, 102, 124) inclui um furo atravessante de respiro (48, 80, 92, 98), o eletrodo plano (32, 70, 94, 112, 114) estando em uma relação laminada entre o primeiro suporte plano de amostra (50, 72, 104, 122) e o segundo suporte plano (40, 78, 96, 102, 124), adicionalmente caracterizado pelo fato de que o furo atravessante de amostra (56, 74, 90), o furo atravessante do eletrodo (38, 76) e o furo atravessante de respiro (48, 80, 92, 98) estão em uma disposição de superposição. Preferivelmente, alguns ou todos os furos atravessantes, regiões atravessantes e furos atravessantes de respiro são alinhados. Em uma concretização, a dimensão lateral do furo atravessante de respiro é maior do que a dimensão lateral do furo atravessante de eletrodo. Em uma concretização alternativa, a dimensão lateral do furo atravessante de amostra é maior do que a dimensão lateral do furo atravessante de respiro. Na concretização preferida, o suporte de amostra e o suporte plano são formados de material em folha, mais preferivelmente de polimida, tendo uma espessura substancialmente de 1 a substancialmente 5 mils. Os eletrodos são preferivelmente escolhidos a partir de metais nobres, especialmente o ouro. Os furos ou regiões atravessantes são preferivelmente formadas por perfuração a laser, opcionalmente seguida por causticação química. Vias de interconexão apresentam percursos condutivos através dos múltiplos suportes, e s ...

Counting mechanism for printing machines

Improvements in Padded Horse Shoes.

15,440. Sheldon, C. E. June 29. India-rubber with embedded bars; hoof pads.-Fig. 2 shows the upper or hoof side of the shoe, with part of the upper covering removed, and Fig. 3 shows a side view of the shoe. The shoe consists of a metal part A embedded in rubber &c. R which is extended at the heels to form cushions G bearing on part of the frog. The rubber beneath the shoe tapers off at the toe where the wear is greatest. The rubber above the shoe is covered with canvas H, and is preferably strengthened by perforated metal plates D, E, F. The shoe is recessed at B, and preferably perforated at various points U to give the rubber a better hold.

Numbering mechanism.

Improvements in and relating to Spring Wheels.

10,694. Sheldon. E. N. May 9. Spring wheels; spokes; felloes or rims.-The spokes of a wheel for bicycles, automobiles, &c. are movable at their outer ends in tubular casings 8 fixed to the inner part 2 of a hollow rim. The outer rim part 1 is braced by a V-section ring 5 fastened to it by rivets 7 or the like. Each spoke is fixed by a nut 18 on its threaded end to a sleeve 9, which is pressed outwards by a spring 12 contained in a cloeed oil chamber formed by a stuffing- box 13, 15 and the head 10 of the sleeve. The outer rim part 1 may have spaced diagonal ribs on the tread.

Machine for wrapping pamphlets or similar articles.

Staple-forming mechanism.

Labeling-machine.

Rotary printing-machine.

Delivery mechanism for folded products.