Method to produce inactivated w/o emulsion adjuvated vaccines

Immunogenic composition of killed Leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilize an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.

Immunogenic composition of killed leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilize an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.

IMMUNOGENIC COMPOSITION OF KILLED LEPTOSPIRA BACTERIA

The present invention pertains to a composition containing an immunogenic cell preparation of killed bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilise an immunogenic preparation of killed bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier. 111-. (canceled)12Leptospira. A composition containing an immunogenic cell preparation of killed bacteria in an ethylenediaminetetraacetic acid solution.13. The composition of claim 12 , wherein the solution comprises 5 to 50 mmols of ethylenediaminetetraacetic acid per litre.14. The composition of claim 13 , wherein the solution comprises 20 mmols of ethylenediaminetetraacetic acid per litre.15. The composition of claim 14 , wherein the solution contains dibasic sodium phosphate.16. The composition of claim 13 , wherein the solution contains dibasic sodium phosphate.17. The composition of claim 12 , wherein the solution contains dibasic sodium phosphate.18LeptospiraLeptospira interrogansLeptospira interrogansLeptospira kirschneri. The composition of claim 17 , wherein the bacteria are selected from the group consisting of serogroup Canicola serovar Portland-Vere claim 17 , serogroup Icterohaemorrhagiae serovar Copenhageni and serogroup Grippotyphosa serovar Dadas.19LeptospiraLeptospira interrogansLeptospira interrogansLeptospira kirschneri. The composition of claim 12 , wherein the bacteria are selected from the group consisting of serogroup Canicola serovar Portland-Vere claim 12 , serogroup Icterohaemorrhagiae serovar Copenhageni and serogroup Grippotyphosa serovar Dadas.20LeptospiraLeptospira interrogans. The composition of claim 19 , wherein the bacteria are serogroup Canicola serovar Portland-Ver.21. A vaccine to protect an animal against an ...

IMMUNOGENIC COMPOSITION OF KILLED LEPTOSPIRA BACTERIA

The present invention pertains to a composition containing an immunogenic cell preparation of killed bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilise an immunogenic preparation of killed bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier. 111-. (canceled)12Leptospira. A composition containing an immunogenic cell preparation of killed bacteria in an ethylenediaminetetraacetic acid solution.13. The composition of claim 12 , wherein the solution comprises 5 to 50 mmols of ethylenediaminetetraacetic acid per litre.14. The composition of claim 13 , wherein the solution comprises 20 mmols of ethylenediaminetetraacetic acid per litre.15. The composition of claim 14 , wherein the solution contains dibasic sodium phosphate.16. The composition of claim 13 , wherein the solution contains dibasic sodium phosphate.17. The composition of claim 12 , wherein the solution contains dibasic sodium phosphate.18LeptospiraLeptospira interrogansLeptospira interrogansLeptospira kirschneri. The composition of claim 17 , wherein the bacteria are selected from the group consisting of serogroup Canicola serovar Portland-Vere claim 17 , serogroup Icterohaemorrhagiae serovar Copenhageni and serogroup Grippotyphosa serovar Dadas.19LeptospiraLeptospira interrogansLeptospira interrogansLeptospira. The composition of claim 12 , wherein the bacteria are selected from the group consisting of serogroup Canicola serovar Portland-Vere claim 12 , serogroup Icterohaemorrhagiae serovar Copenhageni and kirschneri serogroup Grippotyphosa serovar Dadas.20LeptospiraLeptospira. The composition of claim 19 , wherein the bacteria are interrogans serogroup Canicola serovar Portland-Ver.21. A vaccine to protect an animal against an ...

Immunogenic composition of killed leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in a citric acid solution. It also pertains to a vaccine to protect an animal against an infection with Leptospira bacteria, wherein the vaccine comprises this composition, and to the use of citric acid to stabilize an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the citric acid in the carrier.

Immunogenic composition of killed leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilise an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.

Method to produce inactivated w/o emulsion adjuvated vaccines

The present invention is directed to a method to prepare an inactivated w/o emulsion adjuvated vaccine, wherein an aqueous solution comprising one or more inactivated angigens is mixed under mild conditions with a ready-made w/o emulsion. Preferably the vaccine is prepared just prior to vaccination. The aqueous solution and w/o emulsion are stirred or shaken by mechanical means or by hand. The present invention also relates to a kit of parts for use in a method according to the invention.

Immunogenic composition of killed leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilise an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.

Tocols as adjuvant in vaccine

Method to produce inactivated w/o emulsion adjuvanted vaccines

The present invention is directed to a method to prepare an inactivated w/o emulsion adjuvated vaccine, wherein an aqueous solution comprising one or more inactivated angigens is mixed under mild conditions with a ready-made w/o emulsion. Preferably the vaccine is prepared just prior to vaccination. The aqueous solution and w/o emulsion are stirred or shaken by mechanical means or by hand. The present invention also relates to a kit of parts for use in a method according to the invention.

An immunogenic composition of killed leptospira bacteria

The present invention pertains to a composition containing an immunogenic cell preparation of killed Leptospira bacteria in an ethylenediaminetetraacetic acid solution. The invention also pertains to a vaccine to protect an animal against an infection with leptospira bacteria, wherein the vaccine comprises this composition. Also, the invention pertains to the use of ethylenediaminetetraacetic acid to stabilise an immunogenic preparation of killed Leptospira bacteria in a liquid carrier, by dissolving the ethylenediaminetetraacetic acid in the carrier.

Method to produce inactivated w/o emulsion adjuvated vaccines

The present invention is directed to a method to prepare an inactivated w/o emulsion adjuvated vaccine, wherein an aqueous solution comprising one or more inactivated angigens is mixed under mild conditions with a ready-made w/o emulsion. Preferably the vaccine is prepared just prior to vaccination. The aqueous solution and w/o emulsion are stirred or shaken by mechanical means or by hand. The present invention also relates to a kit of parts for use in a method according to the invention.

IMMUNOGENIC COMPOSITION OF KILLED LEPTOSPIRA BACTERIES

Tocols as adjuvant in vaccine

Oil based vaccines often cause serious local side effects at the site of administration and/or have a high viscosity which make said vaccines difficult to handle. Vaccines comprising as an adjuvant a stable emulsion of tocols in water do not display these undesired effects and induce good immune responses.

Method for preparing a vaccine aided by inactivated water / oil emulsion, and a set of parts that can be used for the preparation of a vaccine aided by inactivated water / oil emulsion

"MéTODO PARA PREPARAR UMA VACINA AUXILIADA POR EMULSãO áGUA/óLEO INATIVADA, E, CONJUNTO DE PARTES QUE PODEM SER USADAS PARA A PREPARAçãO DE UMA VACINA AUXILIADA POR EMULSãO áGUA/óLEO INATIVADA" A presente invenção é dirigida a um método de preparar uma vacina auxiliada por emulsão água/óleo inativada, em que uma solução aquosa, compreendendo um ou mais antígenos inativados, é misturada sob condições suaves com uma emulsão de água/óleo pronta. Preferivelmente, a vacina é preparada logo antes da vacinação. A solução aquosa e a emulsão de água/óleo são agitadas ou sacudidas por meios mecânicos ou manualmente. A presente invenção também refere-se a um conjunto ou partes para uso em um método de acordo com a invenção.

COMBINED VACCINE.

Verfahren zur herstellung von inaktivierten, w/ö emulsion adjuvierten, impfstoffen