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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 29. Отображено 29.
22-08-2023 дата публикации

Load-bearing detection cable for ultra-deep well drilling

Номер: CN116631697A
Автор: TAO MANHUA, WANG KEHONG, FANG FENG, QIAO WENWEI, YANG HENGYONG, WU KAIMING, JIANG SHUQING, YE XUEFU, WANG XIAOYU, ZHU TAO, SHEN AIHONG, WU JIAN, FENG ZILI, PANG HAOQIANG, LIU MAN, YUAN QING, WANG QIANG, LI SHUSHENG
Принадлежит:

The invention relates to the technical field of oil and gas well logging, in particular to a load-bearing detection cable for ultra-deep well drilling, the length of the load-bearing detection cable is larger than a preset value, and the load-bearing detection cable comprises a cable core; the insulating layer coats the outer surface of the cable core; the first electromagnetic shielding layer coats the outer surface of the insulating layer; wherein the first electromagnetic shielding layer comprises a first electromagnetic shielding material film layer, a non-electromagnetic shielding material film layer and a second electromagnetic shielding material film layer which are stacked in sequence; the thickness of the first electromagnetic shielding material film layer and the thickness of the second electromagnetic shielding material film layer are 50-100 microns, and the thickness of the non-electromagnetic shielding material film layer is 1-5 mm. And the armor layer coats the outer surface ...

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Номер записи: 1
05-08-2015 дата публикации

Applications of verticillium dahlia pathogenicity related gene VdPR1 as anti-verticillium dahlia target gene

Номер: CN104818287A
Автор: ZHANG YALIN, ZHU HEQIN, LI ZHIFANG, FENG ZILI, FENG HONGJIE, SHI YONGQIANG, ZHAO LIHONG
Принадлежит:

The present invention relates to the field of plant diseases, particularly to applications of verticillium dahlia pathogenicity related gene VdPR1 as the anti-verticillium dahlia target gene. According to the present invention, the colony morphology, the growth rate, the extracellular enzyme activity and the pathogenicity of the knockout mutant [delta]VdPR1 and the complementary mutant [delta]VdPR1-C are determined, and are subjected to compared analysis with various indexes of the wild type, such that it is determined that the VdPR1 gene is the verticillium dahlia pathogenicity related gene, is associated with microsclerotium production and melanin accumulation, and provides promotion effects for activities of cellulase and protease.

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Номер записи: 2
11-04-2023 дата публикации

Compound microbial agent for preventing and treating cotton verticillium wilt and preparation method thereof

Номер: CN115948279A
Автор: ZHANG YALIN, WANG DAGUANG, LIU YUANYUAN, CHE LEI, LIANG WEI, ASHAN. HABUDAN, LIU AIZHONG, FENG HONGJIE, FENG ZILI, WANG BAOXIA, LI YINGLI, ZHANG XIA
Принадлежит:

The invention discloses a compound microbial agent for preventing and treating cotton verticillium wilt and a preparation method thereof.The compound microbial agent is prepared from, by weight, 20-60 parts of microbial powder, 40-70 parts of growth auxiliary materials and 2-8 parts of drying agents, and the microbial powder comprises fusarium, trichoderma harzianum, lactic acid bacteria, bacillus cereus and bacillus velezensis; the preservation number of the fusarium is CGMCC (China General Microbiological Culture Collection Center) NO.11314 Fusarium and trichoderma harzianum in the compound microbial agent have an antagonistic effect on cotton verticillium wilt bacteria, lactic acid bacteria, bacillus cereus and bacillus velezensis are soil beneficial bacteria, and metabolites of the lactic acid bacteria, the bacillus cereus and the bacillus velezensis contain regulating substances such as auxin, antibiotics and cytokinin which are necessary for crop growth. In addition, the strain also ...

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Номер записи: 3
10-02-2016 дата публикации

ATP speedtransmitter installs detection device

Номер: CN0205027280U
Автор: LIANG XIAOYAN, DAI WOLONG, ZHANG FENG, WANG YINLING, ZHANG ZHIYI, DONG CUIYAN, FENG ZILI, SHAO SONGTAO, XU FENG, ZHAO WENHUA, YANG HONGLI, SUN DAOYONG
Принадлежит:

The utility model provides a ATP speedtransmitter installs detection device, ATP speedtransmitter is through conversion bedplate fixed mounting, detection device includes the dipperstick, the bottom of dipperstick is fixed with the locating piece, and it has the slidable vernier scale to overlap on the blade of dipperstick, the bottom of vernier scale is fixed with removable guide holder. The utility model provides a ATP speedtransmitter installs detection device can realize the accuracy and carry out ATP speedtransmitter installation clearance measuring detection device, the device simple structure, and measured data is accurate, avoids human error.

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Номер записи: 4
07-07-2010 дата публикации

Method for synchronously separating tetrahydropalmatine and fumarine from fumitory

Номер: CN0101768159A
Автор: FENG ZILI, JIANG HAI, LI XINSHENG, WU SANQIAO, ZHANG CHENLU, ZILI FENG, HAI JIANG, XINSHENG LI, SANQIAO WU, CHENLU ZHANG
Принадлежит:

The invention discloses a method for synchronously separating tetrahydropalmatine and fumarine from fumitory, comprising the following steps of: preparing the fumitory into total alkali; separating the total alkali by adopting a high-speed counter-current chromatography; distributing a two-phase solvent system with petroleum ether-ethyl acetate-methanol-water, wherein the distribution (volume) ratio is (22-26):(25-30):(20-23):(17-20), an upper phase is a stationary phase and a lower phase is a mobile phase; dissolving fumarine total alkali with the mobile phase; collecting wave crest components of the fumarine at 105-120min; and collecting wave crest components of the tetrahydropalmatine at 165-170min. A fumitory alcohol extract is purified into total alkaloid and the selected solvent system and the mixture ratio are proper, so that the separating degree is high, the purity of separating components is as high as up to 98 percent, the yields of the etrahydropalmatine and the fumarine respectively ...

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Номер записи: 5
12-03-2014 дата публикации

Cotton endophytic fungus CEF-818 and application thereof in control of cotton verticillium wilt

Номер: CN103627643A
Автор: ZHU HEQIN, LI ZHIFANG, FENG ZILI, WANG LINGFEI, ZHENG HONGSHUANG, SHI YONGQIANG, ZHAO LIHONG
Принадлежит:

The invention relates to phytopathology, and in particular relates to cotton endophytic fungus CEF-818 and application thereof in control of cotton verticillium wilt. The cotton endophytic fungus CEF-818 has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 8320. The metabolite of CEF-818 can inhibit cotton verticillium wilt in 100%, and the volatile metabolite of CEF-818 can inhibit cotton verticillium wilt in 77.5%. The greenhouse test result shows that 25% of CEF-818 culture filtrate can control cotton verticillium wilt in 66.2% by soaking seeds, therefore, the CEF-818 has a remarkable effect on controlling verticillium wilt. Compared with the bacillus subtilis widely used in production, the cotton endophytic fungus CEF-818 can increase the control effect by about 25.1%, has no toxic or side effects for the growth of crops, is safe to human and livestock, insects and crops, and is environment-friendly.

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Номер записи: 6
18-07-2023 дата публикации

Grain and medicine interplanting method for rhubarb

Номер: CN116439086A
Автор: MA ZHONGHUA, WANG FENGCHEN, PANG ZHU, FENG ZILI
Принадлежит:

The invention relates to the technical field of Chinese herbal medicine planting methods, in particular to a grain and medicine interplanting method for rhubarb. The method specifically comprises the following steps: land selection and preparation; cultivating the rhubarb; corn interplanting; field management; and harvesting grain and pesticide. The method has the advantages that temperature, light, water and land resources can be fully utilized, on the premise that the planting density and yield of rheum officinale are not reduced in the same area, grain crops are interplanted, and the economic benefit of unit area is greatly improved; after corn interplanting, a dark and humid environment beneficial to rhubarb growth is artificially created, rhubarb growth is facilitated, and the yield and quality of rhubarb can be improved; the method is scientific and reasonable, economic benefits are greatly increased, and popularization is easy. And an auxiliary machine for non-single-row planting ...

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Номер записи: 7
25-07-2012 дата публикации

Preparation method of biological diesel

Номер: CN0101982540B
Автор: XINSHENG LI, QINGBO WU, SANQIAO WU, CHENG LIU, LIN WU, ZHIJIAN ZHANG, ZILI FENG, ZHONGFU HOU, XIAOWEN QU, TING WU, MIN WU, SHECHENG WU, JIANCHENG JI, MINGCHENG WU, WENBIN JIANG, LI XINSHENG, WU QINGBO, WU SANQIAO, LIU CHENG, WU LIN, ZHANG ZHIJIAN, FENG ZILI, HOU ZHONGFU, QU XIAOWEN, WU TING, WU MIN, WU SHECHENG, JI JIANCHENG, WU MINGCHENG, JIANG WENBIN
Принадлежит:

The invention discloses a preparation method of biological diesel. The method comprises the following steps: adding methanol and raw oil (various preprocessed greases) into a reaction kettle in the mol ratio of (4:1)-(8:1), adding acid, and carrying out pre-esterification under negative pressure; adding water to neutralize and wash, dehydrating, removing impurities, drying, sending into the reaction kettle, heating to 75 DEG C, adding alkali, and carrying out transesterification under negative pressure; separating out the glycerol methanol solution on the lower layer to obtain crude biological diesel; and carrying out tertiary molecular distillation to obtain fatty acid methyl ester containing C16, C18 and C20, thereby obtaining the biological diesel. The invention has the advantages thatthe method is simple and the overall esterification time is shortened by 1/2; the transesterification rate is higher than 93%; the dehydration, decoloring, degassing and other working procedures of the ...

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Номер записи: 8
19-03-2014 дата публикации

Cotton endophytic fungi CEF-714 and application of cotton endophytic fungi CEF-714 to preventing and treating cotton verticillium wilt

Номер: CN103642704A
Автор: ZHU HEQIN, FENG ZILI, LI ZHIFANG, WANG LINGFEI, ZHAO LIHONG, SHI YONGQIANG, ZHENG HONGSHUANG
Принадлежит:

The invention relates to phytopathology and particularly relates to cotton endophytic fungi CEF-714 and an application of cotton endophytic fungi CEF-714 to preventing and treating cotton verticillium wilt. The preservation number of the cotton endophytic fungi CEF-714 is CGMCC (China General Microbiological Culture Collection Center) No.8304. The CEF-714 can be cultured on an artificial culture medium and is easy to obtain and culture, so that the industrial production as well as the popularization and the application are facilitated. Indoor bacteriostatic tests show that the inhibition effect on the cotton verticillium wilt by metabolites of the CEF-714 is 100%; the inhibition effects on the cotton verticillium wilt by generated volatile metabolites is 77.5%. A room temperature test result shows that the prevention and treatment effect on the cotton verticillium wilt by using 25% CEF-714 culture filtering solution to immerse seeds is 60.6%; the control effect on the verticillium wilt ...

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Номер записи: 9
11-11-2015 дата публикации

Wireless infusion intelligent monitoring and alarm device based on WIFI

Номер: CN0204745207U
Автор: ZHAO PENG, ZHONG YINBAO, FENG ZILI, WANG WENTAO, LIU JIULING
Принадлежит:

The utility model discloses a wireless infusion intelligent monitoring and alarm device based on WIFI, including the other calling system host computer of bed, hold with the master control that the other calling system host computer of bed is connected to and at least one is held with infusion monitoring and warning that the master control end is connected, liquid level signal acquisition module, signal processing module, berth coding module, photoelectricity alarm module, a WIFI module and a power module are drawn together to infusion monitoring and warning hand ladle, and signal processing module is connected with liquid level signal acquisition module, berth coding module, photoelectricity alarm module, a WIFI module respectively, and the berth coding module is connected with a WIFI module, the 2nd WIFI module, berth decoding module, control module and the 2nd power module are drawn together to the master control hand ladle, and berth decoding module is connected with the 2nd WIFI module ...

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Номер записи: 10
09-09-2015 дата публикации

Application of active polysaccharides in corn top rot control

Номер: CN104886089A
Автор: GUO HONGFU, ZHU HEQIN, LI ZHENGDAO, CAO YUNHUI, SUN HUATIAN, REN YINGDANG, GAO HONGZE, SUN GANGQIANG, WANG LIUMEI, XUE ZHONGLI, FENG ZILI, WANG XIANGPING, LIU HONGJUN, HU YUXIANG, CHEN LUTING, LIU XIAOFAN, GUO HUI, ZHOU JUANLI, ZHAO PING, ZHANG XIAOYING, LIANG BING
Принадлежит:

The invention relates to application of active polysaccharides in corn top rot control. Active polysaccharide 1.0-361ppm is used dependently foliar spray, combined with soluble salt containing plant nutrition in use or combined with pesticide in use, and is evident in corn top rot control effect. The active polysaccharide comprises chitosan oligosaccharide and glucosan and has evident resistance induction activity on crops, environmental stress resistance is enhanced by inducing basic resistance, and occurrence and development of corn top rot are prevented and controlled.

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Номер записи: 11
04-06-2014 дата публикации

Endophytic fungi CEF-642 of cotton and application thereof

Номер: CN103834580A
Автор: LI ZHIFANG, ZHU HEQIN, FENG ZILI, ZHAO LIHONG, SHI YONGQIANG
Принадлежит:

The invention relates to the phytopathology, and particularly relates to cotton endophytic fungi CEF-642 for controlling cotton verticillium wilt. The CEF-642 can be cultured on an artificial culture medium, no special requirements are imposed on the culture conditions, and the industrialized production and popularization and application are facilitated. The indoor bacteriostasis test indicates that the verticillium wilt control effect is good. The confront culture result shows that the inhibition ratio of CEF-642 on the cotton verticillium wilt can reach 83.8%, the inhibition ratio of the volatile metabolic product on the mycelial growth of verticillium lecanii is 81.6%, and the non-volatile metabolic product can completely inhibit the growth of verticillium lecanii. The greenhouse test result shows that the occurrence of verticillium wilt can be controlled well by soaking the cotton seeds with the culture filtrate of CEF-642, and the best control effect can reach 75%; the control effect ...

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Номер записи: 12
25-02-2015 дата публикации

Taper hole image acquisition device of coupling, fitting rate detection method and system

Номер: CN104376562A
Автор: SUN DAOYONG, FENG ZILI, ZHANG FENG, LI ZHAO, XU FENG, SHAO SONGTAO, CAO RUI, GUAN MING
Принадлежит:

The invention provides a taper hole image acquisition device of a coupling. The taper hole image acquisition device of the coupling comprises a platform used for bearing a hub of the coupling, a vertical guide rail is arranged on the platform, a transverse supporting rod in sliding connection with the guide rail is arranged at the upper end of the guide rail, a rotary table is arranged at the other end of the supporting rod, a rotary component of the rotary table is connected with a camera supporting rod perpendicular to the platform, a camera is arranged at the lower end of the camera supporting rod, and the camera is located inside the hub, faces towards the inner wall of the hub, and is used for collecting a taper hole image. According to the taper hole image acquisition device of the coupling, the camera is utilized to extend into a shaft hole of the hub of the coupling to carry out image collection on the inner wall of the shaft hole, and therefore a complete shaft hole image can be ...

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Номер записи: 13
07-07-2023 дата публикации

Control method and control system of multispectral constant-temperature and constant-humidity equipment

Номер: CN116399013A
Автор: HU DAIHUA, WANG YULIN, FENG ZILI, CHEN WANG
Принадлежит:

The control method comprises the following steps that S1, an outer fan adjusts the internal temperature of the constant-temperature and constant-humidity equipment to be within a first preset error range of a specified temperature at a first rotating speed, and the air volume needed for maintaining the specified temperature is calculated based on the internal volume of the constant-temperature and constant-humidity equipment, determining a second rotating speed of the outer fan according to the air volume; and S2, after the outer fan runs for specified time at the second rotating speed, whether the internal temperature of the constant-temperature and constant-humidity equipment is within a second preset error range of the specified temperature or not is judged, the rotating speed of the outer fan can be reduced at the first time, the effect of rapid noise reduction is achieved, and meanwhile the effect of rapid noise reduction is achieved through secondary rotating speed adjustment. The ...

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Номер записи: 14
12-03-2014 дата публикации

Running-in test device for built-in axle boxes of rail vehicle

Номер: CN103630342A
Автор: ZHANG FENG, SHAO SONGTAO, FENG ZILI, XU FENG, SUN DAOYONG, NIU FUJIE, CAO RUI
Принадлежит:

The invention relates to a running-in test device for built-in axle boxes of a rail vehicle, comprising axle boxes and an axle passing through the axle boxes and further comprising a platform, supporting seats, axle box positioning seats, a driving unit, axle box temperature detection devices and a control device, wherein the supporting seats are fixedly installed on the platform, the axle box positioning seats are installed on the supporting seats, the axle boxes are fixed on the axle box positioning seats, one end of the axle is connected with the driving unit, and the driving unit and the axle box temperature detection devices are connected with the control device. The running-in test device has the advantages of simple structure, convenient operation and high degree of automation. Running-in test of built-in axle boxes of a rail vehicle after assembly is well implemented. The running-in test device can detect the temperature of bearings of the axle boxes in real time in the testing ...

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Номер записи: 15
09-09-2015 дата публикации

Application of potassium permanganate in controlling corn top rot

Номер: CN104886139A
Автор: GUO HONGFU, ZHU HEQIN, SUN HUATIAN, LI ZHENGDAO, CAO YUNHUI, REN YINGDANG, GAO HONGZE, SUN GANGQIANG, XUE ZHONGLI, FENG ZILI, WANG XIANGPING, HU YUXIANG, CHEN LUTING, LIU XIAOFAN, GUO HUI, DONG HONGMIN, ZHAO PING, YUE ZHENGUO, SHANG HONGYAN, YAN SHUHUA, LI GUOSHENG
Принадлежит:

The invention relates to application of potassium permanganate in controlling corn top rot. According to an application method, potassium permanganate 246 to 3277 ppm in concentration is subjected to leaf spraying or root irrigation and is available for independent use, or the potassium permanganate is used with soluble salt containing plant nutrition or with pesticide; the potassium permanganate has evident effect of controlling the corn top rot. The potassium permanganate has evident resistance induction action on crops; by inducing basic resistance, the ability to resist environmental stress is enhanced, and the occurrence and development of the corn top rot is prevented and controlled.

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Номер записи: 16
20-04-2011 дата публикации

Quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper

Номер: CN0102021223A
Автор: Zhu Heqin, Feng Zili, Zhao Lihong, Shi Yongqiang, Li Zhifang
Принадлежит:

The invention relates to the plant pathology field, and specially relates to a quantitative dip in bacterial suspension method for identifying resistance to cotton verticillium wilt using vermiculite sandy soil bottomless bowl of paper. The method in the invention includes the following steps: 1) making a nutrient bowl by mixing vermiculite and sandy soil and putting them into a paper bowl; 2) sowing and culturing cotton sprouts; 3) inoculating pathogen, which requires inoculating bacterium spore suspension of cotton verticillium wilt when one true leaf of a cotton sprout grows to be flat, wherein the inoculation concentration is in the range of from 1*10<6> to 5*10<6> spores per ml, and every cotton sprout is inoculated with bacteria liquid about from 2 to 3 ml; 4) evaluating the resistance to cotton verticillium wilt. The invention is characterized by being fast, accurate, economical, environmental beneficial and so on.

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Номер записи: 17
02-03-2011 дата публикации

Preparation method of biological diesel

Номер: CN0101982540A
Автор: XINSHENG LI, QINGBO WU, SANQIAO WU, CHENG LIU, LIN WU, ZHIJIAN ZHANG, ZILI FENG, ZHONGFU HOU, XIAOWEN QU, TING WU, MIN WU, SHECHENG WU, JIANCHENG JI, MINGCHENG WU, WENBIN JIANG, LI XINSHENG, WU QINGBO, WU SANQIAO, LIU CHENG, WU LIN, ZHANG ZHIJIAN, FENG ZILI, HOU ZHONGFU, QU XIAOWEN, WU TING, WU MIN, WU SHECHENG, JI JIANCHENG, WU MINGCHENG, JIANG WENBIN
Принадлежит:

The invention discloses a preparation method of biological diesel. The method comprises the following steps: adding methanol and raw oil (various preprocessed greases) into a reaction kettle in the mol ratio of (4:1)-(8:1), adding acid, and carrying out pre-esterification under negative pressure; adding water to neutralize and wash, dehydrating, removing impurities, drying, sending into the reaction kettle, heating to 75 DEG C, adding alkali, and carrying out transesterification under negative pressure; separating out the glycerol methanol solution on the lower layer to obtain crude biological diesel; and carrying out tertiary molecular distillation to obtain fatty acid methyl ester containing C16, C18 and C20, thereby obtaining the biological diesel. The invention has the advantages that the method is simple and the overall esterification time is shortened by 1/2; the transesterification rate is higher than 93%; the dehydration, decoloring, degassing and other working procedures of the ...

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Номер записи: 18
04-08-2023 дата публикации

Load bearing detection cable

Номер: CN116543969A
Автор: TAO MANHUA, SHEN AIHONG, QIAO WENWEI, YANG HENGYONG, WANG KEHONG, FANG FENG, WU KAIMING, JIANG SHUQING, YE XUEFU, WANG XIAOYU, WU JIAN, FENG ZILI, ZHOU CHUNHE, PANG HAOQIANG, LIU MAN, YUAN QING, WANG QIANG, LI SHUSHENG
Принадлежит:

The invention relates to a load bearing detection cable, which comprises a cable core, the cable core comprises a power line for providing a power supply for a logging instrument, a signal line for transmitting a logging signal output by the logging instrument, and a first shielding layer wrapping the outer surface of the power line, and the inner side of the first shielding layer is provided with an electromagnetic wave energy conversion material layer; the insulating layer coats the outer surface of the cable core; the second shielding layer coats the outer surface of the insulating layer; and the armor layer coats the outer surface of the second shielding layer. According to the load-bearing detection cable disclosed by the invention, the influence of electromagnetic interference generated by the power line on the transmission of the logging signal by the signal line can be reduced to the greatest extent, the transmission performance of the load-bearing detection cable for transmitting ...

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Номер записи: 19
26-03-2014 дата публикации

Bearing, shaft box and accessory washing device and method

Номер: CN103658098A
Автор: ZHAO WENHUA, DONG CUIYAN, ZHANG FENG, LIU YONGBIN, FENG ZILI, SHAO SONGTAO
Принадлежит:

The invention relates to a bearing shaft box and accessory washing device which comprises an operation table assembly, a transparent splashing-preventing cover, a high-pressure washing gun, a high-pressure air gun, a high-pressure centrifugal pump and a filtering system. The operation table assembly comprises an operation frame and a cleaning agent box body which is arranged on the operation frame. Two net-shaped partitioning plates are arranged on the upper portion of the cleaning agent box body in a step shape. Cleaning agents are arranged in the position, below the partitioning plates, of the cleaning agent box body. The transparent splashing-preventing cover is arranged above the operation table assembly. Two digging hands are arranged at the positions, corresponding to the net-shaped partitioning plates, of the surface of the transparent splashing-preventing cover. Blocking curtains are arranged at the digging hands. The high-pressure washing gun and the high-pressure air gun are fixed ...

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Номер записи: 20
02-07-2014 дата публикации

Endophytic fungi CEF-193 of cotton and application thereof

Номер: CN103897992A
Автор: FENG ZILI, ZHU HEQIN, LI ZHIFANG, HUANG DIANCHENG, WANG LINGFEI, SHI YONGQIANG, ZHAO LIHONG
Принадлежит:

The invention relates to phytopathology, and particularly relates to endophytic fungi CEF-193 of cotton and application thereof. The bacterial strain CEF-193 (Acremonium alternatum) with a significant induced immune function on cotton verticillium wilt is obtained from the endophytic fungi of the cotton by separation, screening, identifying and biological assay of a greenhouse, the preservation number is CGMCC No.8302, and CEF-193 can be cultivated on an artificial medium, and is easily obtained and cultivated. Thus, industrialized production and popularization and application are facilitated. An indoor bacteriostatic test shows that the control effect on greensickness is good, verticillium dahlia is inoculated 4 days after the CEF-193 is firstly inoculated, the prevention and control effect on the cotton verticillium wilt achieves 74.1%, and the endophytic fungi CEF-193 have significant control action on the greensickness. Compared with the bacillus subtilis mainly applied to production ...

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Номер записи: 21
27-05-2015 дата публикации

Primer group and kit for simultaneously detecting four diarrhea viruses

Номер: CN104651538A
Автор: WANG HAIBO, TAN HUA, FENG ZILI, WU BIMEI, LI YANLAN, ZHAO JUNHUA, WANG QI, MO QIUHUA, YANG ZE, LIN JICAN
Принадлежит:

The invention discloses a primer group which is based on a high-resolution melting curve analysis technique and used for simultaneously detecting four diarrhea viruses, and a quadruple detection kit containing primers. The kit is capable of specifically detecting four diarrhea viruses, that is, rotavirus, astrovirus, norovirus and sapovirus. Through comparative analysis on respective homologies of the four viral genomes, respective conserved sequence design primers with high homologies can be found, the four diarrhea viruses can be simultaneously detected by using the high-resolution melting curve analysis technique, the primer group has the characteristics of being simple, convenient, rapid, cheap, safe, high in sensitivity and high in specificity, and powerful technical support can be provided for prevention and control and clinical diagnosis of dysentery.

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Номер записи: 22
02-04-2014 дата публикации

ITS-RFLP method for rapidly identifying main cotton pathogenic fungus

Номер: CN103695533A
Автор: LI ZHIFANG, ZHU HEQIN, FENG ZILI, ZHAO LIHONG, SHI YONGQIANG
Принадлежит:

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming ...

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Номер записи: 23
04-03-2015 дата публикации

Nucleic acid aptamer for combining cholera enterotoxin subunit B and application thereof

Номер: CN104388434A
Автор: MO QIUHUA, TAN HUA, WANG HAIBO, FENG ZILI, YANG ZE, LIN JICAN
Принадлежит:

The invention relates to an oligonucleotide aptamer capable of specifically combining a cholera enterotoxin subunit B and an application thereof. The aptamer is a single-chain DNA molecule with 81 base lengths, and has a nucleotide sequence described in a sequence table SEQ ID No. 1. The aptamer disclosed by the invention is screened by adopting a system evolution index enrichment technology, can be used for preparing a detection reagent of cholera enterotoxin, and has the advantages of stability, simplicity and convenience, high speed, economic performance and the like.

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Номер записи: 24
04-08-2023 дата публикации

Microbial agent for preventing and treating verticillium wilt as well as preparation method and application of microbial agent

Номер: CN116536187A
Автор: WEI FENG, ZHU HEQIN, YANG CHUANZHEN, FENG ZILI, ZHANG YALIN, FENG HONGJIE, ZHAO LIHONG, ZHOU JINGLONG
Принадлежит:

The invention discloses a fungicide for preventing and treating verticillium wilt as well as a preparation method and application thereof, and relates to the technical field of biological prevention and treatment. The bacterial agent for preventing and treating the verticillium wilt comprises one or more of bacillus aerosus CRB-021, pseudomonas meliriaceae CRB-212, lysobacter thickness CRB-253 and trichoderma thickness CRF-014. The bacterial agent for preventing and treating the verticillium wilt comprises one or more of bacillus aerosus CRB-021 and pseudomonas meliriaceae CRB-212. The disease rate of cotton verticillium wilt treated by the compound microbial agent is reduced to 28.08 +/-0.40%, the disease index is reduced to 20.62 +/-1.04 which are respectively reduced by 53.72% and 39.79% compared with those of a control group, and the prevention and treatment effect is up to 68.00 +/-0.59%. Meanwhile, the compound microbial agent also has a remarkable growth promoting effect on germination ...

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Номер записи: 25
30-09-2015 дата публикации

Medical fertilizer mixture for preventing corn top rot

Номер: CN104945132A
Автор: GUO HONGFU, ZHU HEQIN, SUN HUATIAN, LI ZHENGDAO, CAO YUNHUI, XUE ZHONGLI, GAO HONGZE, SUN GANGQIANG, QIN HAIYING, FENG ZILI, WANG LIUMEI, LI QINCUN, HU ZHANJU, JI SHELIN, CHENG XING, YUE ZHENGUO, XIE WENFANG, WANG DAN, QI XIAOGUANG, ZHANG XIAOYING, LIANG BING
Принадлежит:

The invention relates to a medical fertilizer mixture for preventing corn top rot, wherein active ingredients of the medical fertilizer mixture for preventing the corn top rot comprises plant growth adjusting agents and mineral nutrition elements, wherein the plant growth adjusting agents are one or two of compound nitrate phenolate and diethylaminoethyl hexanoate, and the mineral nutrition elements are one or more of middle elements calcium, trace element copper, manganese, zinc soluble salt. The medical fertilizer mixture for preventing the corn top rot synthetically utilizes dual effects of mineral nutrition and induced resistance of the middle elements calcium, the manganese and the zinc, commonly acts with a growth-promoting type growth adjusting agent to induce basic resistance, enhances ability for resisting environmental stress, promotes diseased plants to rapidly recover normal growth, and prevents and controls the corn top rot to happen and develop.

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Номер записи: 26
26-03-2014 дата публикации

Method for preparing special bio-organic fertilizer for tea by using plant extract residues

Номер: CN103664389A
Автор: FENG ZILI, YAO XIAORU, CHEN LICHENG, JI XIAOHUI, CHEN DEJING, XIE XIUCHAO, FU JING
Принадлежит:

The invention relates to a method for preparing a special bio-organic fertilizer for tea by using plant extract residues. The special bio-organic fertilizer comprises the following raw materials: epimedium extract residues, rhodiola rosea extract residues, pig manure, sheep manure, calcium superphosphate and an EM (effective microorganisms) solution, wherein the materials are added with cellulose and uniformly mixed, and then subjected to piling up, enzymolysis, grinding, fermentation and purification to prepare the fertilizer. The method for preparing the special bio-organic fertilizer for tea by using the plant extract residues has the characteristics as follows: 1, a large quantity of residues generated in production processes of an epimedium extract and a rhodiola rosea extract can be treated stably and effectively, and the fibrinolysis level of follow-up fermentation as well as C and N resource utilization rate can be improved effectively through pre-treatment of cellulose; 2, a production ...

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Номер записи: 27
30-09-2015 дата публикации

Pesticide combination for preventing corn top rot

Номер: CN104938491A
Автор: GUO HONGFU, ZHU HEQIN, SUN HUATIAN, LI ZHENGDAO, CAO YUNHUI, FENG ZILI, GAO HONGZE, QIN HAIYING, SUN GANGQIANG, XUE ZHONGLI, WANG LIUMEI, LI QINCUN, SHANG HONGYAN, HU ZHANJU, JI SHELIN, CHENG XING, XIE WENFANG, WANG DAN, YUE ZHENGUO, QI XIAOGUANG, LIANG BING
Принадлежит:

The invention relates to a pesticide combination for preventing corn top rot, wherein the effective components of the pesticide combination for preventing the corn top rot comprises plant growth adjusting agents and active polysaccharides, the plant growth adjusting agents are one or two of compound nitrate phenolate and diethylaminoethyl hexanoate, and the active polysaccharides are one or two of chitosan oligosaccharide and portuguese polyene sugar. The pesticide combination for preventing the corn top rot comprises the components with part by weight: compound nitrate phenolate 0.05-90.0 parts and diethylaminoethyl hexanoate 0.05-90.0 parts. The pesticide combination for preventing the corn top rot induces basic resistance of corns by synergistically effects of the plant growth adjusting agents and the active polysaccharides, enhances ability of the corns for resisting environmental stress, promotes diseased plants to rapidly recover normal growth, and prevents and controls the corn top ...

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Номер записи: 28
24-12-2014 дата публикации

Cotton verticillium dahliae pathogenicity-related (VdPR3) protein as well as encoding gene and applications of protein

Номер: CN104231062A
Автор: ZHANG YALIN, ZHU HEQIN, LI ZHIFANG, FENG ZILI, FENG HONGJIE, ZHAO LIHONG, SHI YONGQIANG
Принадлежит:

The invention relates to the field of phytopathology and microbial genetic engineering, and provides a nucleotide sequence of a new verticillium dahliae pathogenicity-related (VdPR3) gene which is derived from cotton verticillium dahliae and plays an important role in pathogenicity, melanin accumulation, microsclerotia generation and cellulase activity, and an amino acid sequence of an encoding protein of the new gene VdPR3. Specifically, the invention discloses a cotton verticillium dahliae pathogenesis-related gene VdPR3. The nucleotide sequence of the gene is shown in SEQ ID NO:1 or SEQ ID NO:2, and the amino acid sequence of the encoded protein sequence of the gene is shown in SEQ ID NO:3. The cotton verticillium dahliae pathogenesis-related gene VdPR3 disclosed by the invention can be used for reducing the pathogenicity of cotton verticillium dahliae.

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Номер записи: 29