14-04-2016 дата публикации
Номер: US20160102344A1
Принадлежит:
The present invention relates to methods for the site-specific immobilization of DNA origami structures on solid substrates, as well as to respective assemblies comprising DNA origami structures immobilized on a solid substrate and uses thereof. 1. A method for the site-specific immobilization of DNA origami structures on a solid substrate , said method comprising the steps of:(a) providing at least one type of DNA origami structure having at least one type of first structural feature;(b) providing a pre-patterned solid substrate that is functionalized with at least one type of a second structural feature, wherein the second structural feature is capable of binding to the first structural feature on the DNA origami structure; and(c) incubating the solid substrate of step (b) with the at least one type of DNA origami structure of step (a).2. The method according to claim 1 , wherein the first structural feature is single-stranded nucleic acid (ssNA) strands protruding from the DNA origami structure and the second structural feature is ssNA capture strands claim 1 , wherein the ssNA capture strands are capable of binding to the ssNA strands protruding from the DNA origami structure by Watson-Crick pairing claim 1 , said method comprising the steps of:(a) providing at least one type of DNA origami structure having single-stranded ssNA strands protruding from the DNA origami structure;(b) providing a pre-patterned solid substrate that is functionalized with ssNA capture strands, wherein the ssNA capture strands are capable of binding to the ssNA strands protruding from the DNA origami structure by Watson-Crick pairing; and(c) incubating the solid substrate of step (b) with the at least one type of DNA origami structure of step (a).3. The method according to claim 2 , wherein the ssNA strands and capture strands are ssDNA strands and capture strands.4. The method according to claim 2 , wherein the region of Watson-Crick pairing between the ssNA/ssDNA strands protruding ...
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