10-09-2015 дата публикации
Номер: US20150252380A1
Принадлежит:
The present invention provides compositions and methods for killing or suppressing growth of Gram-negative bacteria that infect, infest or cause disease in plants, including pathogenic, saprophytic and opportunistic microbes that cause disease in plants and food borne illness in people or in animal feed. 1. A transgenic plant , plant part , plant cell , or plant tissue culture comprising a DNA molecule , wherein the DNA molecule encodes a Bacterial Outer Membrane Breaching (BOMB) polypeptide sharing at least 90% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9 , SEQ ID NO:11 , SEQ ID NO:13 , SEQ ID NO:15 , and SEQ ID NO:17.2. (canceled)3. (canceled)4. The transgenic plant claim 1 , plant part claim 1 , plant cell claim 1 , or plant tissue culture of ; wherein the DNA molecule encodes a BOMB polypeptide sharing at least 95% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9 claim 1 , SEQ ID NO:11 claim 1 , SEQ ID NO:13 claim 1 , SEQ ID NO:15 claim 1 , and SEQ ID NO:17.5. (canceled)6. (canceled)7. The transgenic plant claim 1 , plant part claim 1 , plant cell claim 1 , or plant tissue culture of claim 1 , wherein the DNA encoding the BOMB polypeptide is any codon-optimized version of said DNA.8. (canceled)9. A method for enhancing resistance of a plant to infection or infestation by Gram-negative bacteria claim 1 , said method comprising introducing into the genome of the plant an expression cassette comprising:1) a plant promoter; (a) a nucleic acid sequence encoding a BOMB polypeptide sharing at least 90% amino acid identity with a BOMB polypeptide selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, and SEQ ID NO:17; and', '(b) any codon optimized version of a nucleic acid of a sequence of (a), wherein the nucleic acid sequence is operably fused to said promoter; and, '2) a gene comprising a nucleic acid sequence selected from the group ...
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