Technology for recycling beer from beer waste yeast paste

The invention discloses a technology for recycling beer from beer waste yeast paste, which comprises the steps that the beer waste yeast paste is injected into a circulation tank, and filtered and separated by a metal membrane or a ceramic membrane through a feeding pump, a condenser and a circulation pump; the separated beer penetrating fluid is added to a semi-finished product processing system; a part of waste yeast paste mother liquor returns to the circulation tank, and the other part is filtered by the metal membrane or the ceramic membrane through the circulation pump; and the waste yeast paste is exhausted to enter a deep processing system when the seepage of filter membrane beer is less than 20L/m<2>h. The technology has the advantages of effective separation and clarification of the beer waste yeast paste, high separation speed, high precision, direct elimination of yeast and bacteria, and sterilization technology ignorance, and the separated beer can directly enter a draught ...

Adenylate cyclase mutant, recombinant bacterium and application of adenylate cyclase mutant in cAMP synthesis

The invention discloses an adenylate cyclase mutant, a recombinant bacterium and application of the adenylate cyclase mutant in cAMP synthesis. The amino acid sequence of the mutant is obtained by performing one or more mutations on a sequence as shown in SEQ ID NO.1. The mutation site of the gene comprises one or more of five mutation sites of the 65th site (T65K), the 207th site (E207A), the 304th site (M304I), the 252nd site (Y252F) and the 393th site (S393R). Compared with wild adenylate cyclase, the catalytic activity and the stability of the five adenylate cyclase mutants in a low-temperature environment are remarkably improved. The invention further provides recombinant bacteria of the adenylate cyclase mutant and application of the recombinant bacteria in cAMP synthesis. When the recombinant strain is used for whole-cell catalytic synthesis of cAMP, 20.6 g/L of cAMP can be generated at most, the yield reaches up to 95% or above, and the recombinant strain has a good industrial prospect ...

Method for preparing industrial yeast by use of antibiotic mushroom dregs

The invention discloses a method for preparing industrial yeast by use of antibiotic mushroom dregs. The method comprises the following steps: adding the antibiotic mushroom dregs into a fermentation tank, and configuring to form a water system; ventilating steam into the fermentation tank, sterilizing, and introducing an engineering strain into the fermentation tank so as to decompose antibiotics in the mushroom dregs and remove residues; introducing a yeast strain into the fermentation tank to carry out fermentation to obtain industrial yeast fermentation liquor; and separating and drying the industrial yeast to obtain the industrial yeast. The prepared industrial yeast can be used as a fermentation culture medium of products such as abamectin, tylosin, oxytetracycline and erythromycin or can be used for other industrial purposes. The method disclosed by the invention can be used for realizing innocent treatment and resource utilization of the antibiotic mushroom dregs, can be used for ...

Aspartase mutant and application thereof in synthesis of R-3-aminobutyric acid

The invention provides an aspartase mutant and a method for producing R-3-aminobutyric acid by using the aspartase mutant, and belongs to the field of enzyme engineering. The amino acid sequence of the mutant is obtained by carrying out one or more mutations on the sequence shown in SEQ ID NO.3. The invention relates to a mutation site sequence of the gene, which comprises one or more mutation sites in four mutation sites of 237th site (T237C), 31V site (I31V), 88N site (K88N) and 123 site (G123S). The aspartase mutant disclosed by the invention has the advantages of high catalytic property and high selectivity, and a thallus obtained by fermentation can catalyze a 300g/L substrate, which is the highest substrate concentration reported at present. When the catalyst is used for synthesizing the R-3-aminobutyric acid, the generation rate and chiral purity of the R-3-aminobutyric acid can reach 99% or above within 7 hours. Compared with a previous chemical synthesis method and a biological ...

Process for extracting beer waste yeast fermentation enzymatic hydrolysate

The invention discloses a process for extracting beer waste yeast fermentation enzymatic hydrolysate, which comprises the following steps of: filtering the beer waste yeast fermentation enzymatic hydrolysate by using a metallic film or a ceramic film; putting the yeast extract clear solution after filtering into subsequent processing process steps, returning part of mother liquid to a circulating tank, and performing cycling filtration on the other part of the mother liquid after processing the other part of the mother liquid in a circulating pump; when the percentage of solid substances in the enzymatic hydrolysate reaches 25 to 35 percent, feeding deionized water to perform dialysis; and when the content of yeast extracts in the mother liquid is less than 100 mg/L to 300 mg/L, finishing the extraction process. The process can effectively extract the yeast extract clear solution in the enzymatic hydrolysate and has high separation speed and high accuracy, the yeast extract clear solution ...

Ammoximation reaction and separation coupling process and device

The invention discloses an ammoximation reaction and separation coupling process which comprises the following steps: continuously adding reaction raw materials and a solvent in different groups outside a reaction kettle into a pipeline reactor for reaction, mixing the components in a mixer, and subsequently feeding the mixture into the reaction kettle for sufficient reaction, wherein the upper part of the reaction kettle is a reaction zone, and the lower part of the reaction kettle is a filtration separation zone; separating a reaction product, namely, cyclohexanone-oxime from a catalyst in the filtration separation zone, feeding the separated cyclohexanone-oxime into a later process, and circulating the catalyst back to the reaction kettle for continuous reaction through equipment such as the pipeline reactor outside the reaction kettle. Backwashing is performed once every 10-300 seconds, so that the continuity of reaction separation is ensured. As the raw materials sufficiently contact ...

Toluol-benzene solvent recovery technology from vent gas

The invention discloses a toluene-benzene vent gas recovery process during hexanolactam production by adopting toluene oxidation method, which comprises the following steps: toluene-benzene vent gas discharged from a toluene oxidation device is introduced into an adsorption tower to carry out gas-liquid countercurrent contact with absorbent that is injected form the tower top under the temperature of 20-50 DEG C and pressure of 0.5-1.0MPa; when the adsorption rate of the toluene-benzene vent gas is up to 90-98 percent, the solution absorbed with toluene-benzene is introduced to a desorption tower and desorption is carried out under temperature of 90-108 DEG C and pressure of 0.1-0.12MPa; when the adsorption rate is higher than 95 percent, desorbed toluene-benzene mixture air is cooled to 20-40 DEG C, the adsorbent after desorption is cooled to 20-50 DEG C and recycled. The recovery rate of toluene and benzene of the process of the invention can be more than 90 percent, and the gross content ...

Process for extracting pleuromulin

The invention discloses a process for extracting pleuromutilin in tiamulin synthetic process. The process comprises the following steps: mixing pleuromutilin mycelium with methanol or ethanol, and continuously stirring the mixture at the temperature of 20-40 DEG C for 3-6h to prepare leach liquor; and allowing the leach liquor to pass through a closed inorganic membrane filtering system at a certain pressure, sending the obtained filtrate to a subsequent processing system, allowing part of the residual filtrate to return to a lixiviating tank and the other part thereof to run through a circulating pump for being circularly filtered by the closed inorganic membrane. The process can effectively separate and clarify the pleuromutilin leach liquor, shorten process flow by 6-10h for each day, improve the yield by 5-7% compared with a traditional plate-and-frame filtering process, and has fast filtering rate, high filtering precision and low energy consumption. The closed inorganic membrane filtering ...

Method for preparing epoxypropane

The invention relates to a method for preparing epoxypropane. The method comprises the following steps: adding raw materials propylene and oxydol, a solvent methanol and a titanium silicon molecular sieve catalyst into a reaction kettle according to the mole ratio, and regulating the temperature and pressure in the reaction kettle to establish circulation; continuously adding the raw materials and solvent into the pipeline reactor from the outside of the reaction kettle to react, mixing with a static mixer, and sending into the reaction kettle; discharging the reaction mixture from the bottom of the reaction kettle, delivering to a primary heat exchanger through a circulating pump to perform cooling, separating the product epoxypropane and catalyst in a cross-flow filter membrane separator to obtain the product epoxypropane, and returning the trapped catalyst into the reaction kettle via the pipeline reactor, heat exchanger, static mixer and other apparatuses to form the circulation and ...

Extraction technology of avermectin

Isothermal reactor suitable for strong exothermal reaction

The utility model relates to an isothermal reactor suitable for strong exothermal reaction. The reactor main part is equipped with upper and lower head, advances, discharge gate and coolant import and export, is equipped with a cuff pipe in the reactor main part at least, and every cuff pipe comprises nested within each other's outer tube and inner tube, and on the upper and lower fixed plate in the reactor main part was fixed at the outer tube both ends, the inner tube lower extreme was fixed at the inner tube fixed plate that lies in outer tube bottom plate below, inner tube lower extreme opening, upper end seal and outer tube an upper fixed plate leave the distance within a definite time, the inner tube lateral wall lies in the outer tube bottom plate division has a liquid hole with the upper segment. A reaction of reactant A that when was used for strong exothermal isothermal reaction, a reactant A got into the outer tube through the feed inlet, another reactant B gets into the inner ...