Information exchanging matrix realizing method and module

The client terminal of information exchange is connected to the terminal module and each terminal module has one input port and one output port; and in the addressing module one relatiohship list of input ports and output ports arranged in matrix mode is established and in the list the open-close state of the communication link is recorded in cross mode. After the input terminal module receives the information transfer request, terminal number corresponding to the logic address is obtained from the addressing module and the information is sent directly to the buffer area of destination terminal in output port. The information received by the output port is provided to corresponding client terminal and sent directly to the input buffer area while the response data is returned. The method has fast information exchange speed and is easy to realize in hardware.

Extracting solution for extracting genome DNA (Deoxyribose Nucleic Acid), application of extracting solution and method for rapidly and efficiently extracting genome DNA of tobaccos by utilizing extracting solution

The invention relates to a method for rapidly and efficiently extracting genome DNA of tobaccos. The method comprises the following step: extracting DNA by utilizing an extracting solution, wherein the concentrations of components in the extracting solution are as follows: 0.1mol/L of Tris, 1mol/L of KCl and 10mmol/L of EDTANa2, wherein the pH value of each component is 9.5, and 0.75% (w/v) PVP (polyvinylpyrrolidone) and a 0.36% (w/v) NaHSO3/Na2S2O3 mixture which is formed by mixing the NaHSO3 and the Na2S2O3 according to the weight ratio of 1 to 1 are added into the extracting solution at the same time. Compared with an existing kit used in a laboratory, the method for rapidly and efficiently extracting the genome DNA of tobaccos is less in experimental procedures and simple to operate and is safe because toxic reagents such as chloroform and the like are not adopted in an experimental process; and on the other hand, the method is less in material loss and easily available in reagent, ...

Plant drought, low temperature and osmotic stress induced promoter and application thereof

The invention relates to the field of biotechnology and plant genetic engineering, in particular to a plant drought, low temperature and osmotic stress induced promoter and application thereof. The nucleotide sequence of the promoter is a DNA (deoxyribonucleic acid) sequence as shown in SEQ ID NO. 1. The promoter can drive high expression of an arabidopsis thaliana C2H2-type zinc finger transcription factor protein 5 (AtZAT5) gene under drought and low temperature stress, can be used for improving the growth characteristics and stress tolerance of plants, and has a good application prospect in stress tolerance genetic improvement of the plants.

Molecular marker for identifying synthesis capability of III-VI type sucrose esters of tobacco and application of molecular marker

The invention discloses a molecular marker for identifying the synthesis capability of III-VI type sucrose esters of tobacco and application of the molecular marker. According to the invention, flue-cured tobacco K326 without III-VI type sucrose ester synthesis capability and cigar Beinhart1000-1 with III-VI type sucrose ester synthesis capability are utilized to construct a BC1F1 hybrid population, an interval of III-VI type sucrose ester synthesis genes is positioned on a No.15 chromosome of tobacco, and a molecular marker SNP122 closely linked with the III-VI type sucrose ester synthesis genes is developed. The molecular marker not only can be used for identifying or assisting in identifying the synthesis capability of III-VI type sucrose esters of tobacco to be detected, but also can be used for molecular marker-assisted selective breeding (MAS). The molecular marker disclosed by the invention lays a foundation for positioning cloning of III-VI type sucrose ester synthetic genes, the ...

Tobacco aroma gene and targeting sgRNA and application thereof

The invention provides a tobacco aroma gene as well as targeting sgRNA and application thereof, and belongs to the technical field of tobacco breeding. The invention relates to a tobacco aroma gene, which comprises an NtBADH2a gene and/or an NtBADH2b gene. The nucleotide sequence of the NtBADH2a gene is as shown in SEQ ID NO: 1; the nucleotide sequence of the NtBADH2b gene is as shown in SEQ ID NO: 2. The tobacco fragrance gene is applied to improvement of tobacco fragrance as an action target. In view of the influence of the tobacco aroma gene on the content of tobacco aroma substances through negative regulation, the invention further provides application of the sgRNA of the target tobacco aroma gene or the gene editing system in construction of a tobacco strain rich in aroma substances. Therefore, the tobacco aroma gene provided by the invention provides an effective target for tobacco breeding.

Information exchanging matrix realizing method and system