REAGENT CARTRIDGE AND METHODS FOR DETECTION OF CELLS

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway. 1. A method , comprising:disposing a sample into a reaction chamber, the reaction chamber packaged to contain a reagent formulated to mix with the sample to form an assay media;mixing with the sample in the reaction chamber a plurality of transduction particles associated with a cell phenotype, the plurality of transduction particles engineered to include a nucleic acid molecule formulated to cause the cell phenotype to produce a plurality of reporter molecules capable of generating a detectable signal, the reagent formulated to suppress the detectable signal in a portion of the cell phenotype;receiving a first signal associated with the reagent;maintaining, when the first signal indicates the presence of the reagent, the sample and the plurality of transduction particles to express the plurality of reporter molecules when the cell phenotype is present in the sample; andreceiving a second signal associated with a quantity of the plurality of reporter molecules.2. The method of claim 1 , wherein the reagent is a dried reagent.3. The method of claim 1 , wherein the reagent is adhered to an inner surface of the ...

SYSTEM AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. A method , comprising:mixing with a sample a plurality of transduction particles associated with a target cell, the plurality of transduction particles engineered to include a nucleic acid molecule formulated to cause the target cell to produce a plurality of reporter molecules, the plurality of transduction particles formulated to bind to and deliver the nucleic acid molecule into the target cell, the plurality of transduction particles being non-replicative;maintaining the sample and the plurality of transduction particles to express the plurality of reporter molecules when the target cell is present in the sample; andreceiving a signal associated with a quantity of the plurality of reporter molecules.2. The method of claim 1 , wherein the plurality of transduction particles is particles is derived from a bacteriophage and is engineered to be incapable of lysogenic replication and lytic replication.3. The method of claim 1 , wherein the plurality of transduction particles includes a viral vector devoid of a wild-type DNA capable of exhibiting wild-type viral functions associated with a virus from which the viral vector is ...

Devices and Methods for Biological Assay Sample Preparation and Delivery

Devices and methods for preparing and delivering biological assay samples are provided herein. Components of such devices include a sample receiving module within which a biological assay sample can be prepared and a cap, which when operatively coupled with the sample receiving module, pressurizes the module. These devices can be employed for subsequently delivering a biological assay sample. 1. A biological assay sample preparation device , the device comprising:a. a sample receiving module comprising an outer body forming a first chamber, an inner body forming a second chamber, a preparation solution, and a first attachment element; andb. a cap removably coupleable to the sample receiving module and comprising:i. a pressurizing component; andii. a second attachment element operatively coupleable with the first attachment element,wherein the pressurizing component extends into and pressurizes the sample receiving module upon operative coupling of the first attachment element to the second attachment elementwherein the outer body comprises a piercing member, wherein the inner body comprises a breakable seal and is movable within the outer body, andwherein operatively coupling the first attachment element to the second attachment element comprises moving the inner body within the outer body to break the breakable seal and place the first and second chambers in fluidic communication.2. The device according to claim 1 , wherein the fluid container is adapted to receive one or more portions of a sample.3. The device according to claim 1 , wherein the preparation solution is a nucleic acid amplification preparation solution.4. The device according to claim 1 , wherein the cap comprises a receptacle configured to receive an end of the sample receiving module therein when the cap is coupled to the sample receiving module.5. The device according to claim 4 , wherein the pressurizing component is disposed within the receptacle or integral with the cap.6. (canceled)7. The ...

Devices and Methods for Biological Assay Sample Preparation and Delivery

Devices and methods for preparing and delivering biological assay samples are provided herein. Components of such devices include a sample receiving module within which a biological assay sample can be prepared and a cap, which when operatively coupled with the sample receiving module, pressurizes the module. These devices can be employed for subsequently delivering a biological assay sample. 1. A biological assay sample preparation device , the device comprising:a. a sample receiving module comprising a fluid container, a preparation solution, and a first attachment element; and i. a pressurizing component; and', 'ii. a second attachment element operatively coupleable with the first attachment element,, 'b. a cap removably coupleable to the sample receiving module and comprisingwherein the pressurizing component extends into and pressurizes the sample receiving module upon operative coupling of the first attachment element to the second attachment element.2. The device according to claim 1 , wherein the fluid container is adapted to receive one or more portions of a sample collector.3. The device according to claim 1 , wherein the preparation solution is a nucleic acid amplification preparation solution.4. The device according to claim 1 , wherein the cap comprises a receptacle configured to receive an end of the sample receiving module therein when the cap is coupled to the sample receiving module.5. The device according to claim 4 , wherein the pressurizing component is disposed within the receptacle.6. The device according to claim 1 , wherein the pressurizing element is integral with the cap.7. The device according to claim 1 , wherein the device is a hand-held device.8. The device according to claim 1 , wherein the fluid container has a volume of 50 cmor less.9. The device according to claim 1 , wherein the sample collector comprises a swab.10. The device according to claim 1 , wherein the first attachment element comprises a thread and the second attachment ...

SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. An apparatus , comprising:a housing defining a channel configured to receive a sample container, the housing defining a detection volume configured to place the channel in communication with a detector, the housing including a first seal surface and a second seal surface, a first portion of the sample container and the first seal surface configured to isolate the detection volume from a volume outside of the housing when a second portion of the sample container is disposed within the detection volume; anda shutter having a portion movably disposed within the housing between a first shutter position and a second shutter position, a seal surface of the shutter and the second seal surface configured to isolate the detection volume from the channel of the housing when shutter is in the first shutter position, the channel of the housing being in communication with the detection volume when the shutter is in the second shutter position.2. The apparatus of claim 1 , wherein the first seal surface includes a gasket.3. The apparatus of claim 1 , wherein the shutter is in the first shutter position when the second end portion of the sample ...

SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. A method , comprising:coupling a reagent module to a sample container such that a reaction chamber defined by the sample container is fluidically isolated from an exterior volume, the reaction chamber containing a sample;disposing, after the coupling, at least a distal end portion of the sample container into a detection volume of an instrument;receiving, when the distal end portion of the sample container is in the detection volume, a signal associated with a magnitude of light emission in the detection volume; andactuating, during the receiving, the reagent module to convey a reagent into the reaction chamber of the sample container.2. The method of claim 1 , wherein:the disposing the distal end portion of the sample container includes applying a force on the reagent module such that a portion of the reagent module and a portion of the instrument collectively optically isolate the detection volume, at least a portion of the force being maintained during the receiving.3. The method of claim 2 , wherein:the force is a first force, the first force is applied by an actuator,the actuating the reagent module includes applying, by the ...

SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. A method , comprising:mixing with a sample a plurality of transduction particles associated with a target cell, the plurality of transduction particles engineered to include a nucleic acid molecule formulated to cause the target cell to produce a plurality of reporter molecules, the plurality of transduction particles formulated to bind to and deliver the nucleic acid molecule into the target cell, the plurality of transduction particles being non-replicative;maintaining the sample and the plurality of transduction particles to express the plurality of reporter molecules when the target cell is present in the sample; andreceiving a signal associated with a quantity of the plurality of reporter molecules.2. The method of claim 1 , wherein a magnitude of the signal is independent from a quantity of the plurality of transduction particles above a predetermined quantity.3. The method of claim 1 , wherein the plurality of transduction particles is engineered to be incapable of lysogenic replication.4. The method of claim 1 , wherein the plurality of transduction particles is engineered to be incapable of lytic replication.5. The ...

SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. An apparatus , comprising:a housing defining a channel configured to receive a sample container, the housing defining a detection volume configured to place the channel in communication with a detector, the housing including a first seal surface and a second seal surface, a first portion of the sample container and the first seal surface configured to isolate the detection volume from a volume outside of the housing when a second portion of the sample container is disposed within the detection volume; anda shutter having a portion movably disposed within the housing between a first shutter position and a second shutter position, a seal surface of the shutter and the second seal surface configured to isolate the detection volume from the channel of the housing when shutter is in the first shutter position, the channel of the housing being in communication with the detection volume when the shutter is in the second shutter position.2. The apparatus of claim 1 , wherein the first seal surface includes a gasket.3. The apparatus of claim 1 , wherein the shutter is in the first shutter position when the second end portion of the sample ...

SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. 1. A method of detecting the presence of a target cell within a biological sample using an instrument , comprising:mixing a plurality of transduction particles associated with the target cell with the biological sample, the plurality of transduction particles engineered to be non-replicative and to include a nucleic acid molecule formulated to cause the target cell to produce a plurality of reporter molecules, the plurality of transduction particles formulated to bind to and deliver the nucleic acid molecule into the target cell;maintaining, after the mixing, the biological sample and the plurality of transduction particles for a time period to allow the plurality of reporter molecules to be produced within the biological sample when the target cell is present in the biological sample;placing a reaction chamber containing the biological sample in optical communication with a detector of the instrument;conveying, via a delivery member, a reagent into the reaction chamber, the reagent formulated to react with the plurality of reporter molecules to catalyze production of a signal associated with a quantity of the plurality of the ...

Reagent cartridge and methods for detection of cells

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

Systems and methods for detection of cells using engineered transduction particles

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Reagent cartridge and methods for detection of cells

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

Systems and methods for detection of cells using engineered transduction particles

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Reagent cartridge for detection of cells

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. A delivery portion of the housing defines a delivery path between the reagent volume and the reaction chamber when the housing is coupled to the reaction chamber. The delivery path includes a protrusion such that the delivery path has a discontinuous inner surface. The actuator can be moved to convey a reagent from the reagent container into the reaction chamber via the delivery path.

Reagent cartridge and methods for detection of cells

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

Reagent cartridge for detection of cells

Systems and methods for detection of cells using engineered transduction particles

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Systems and methods for detection of cells using engineered transduction particles

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a targe t cell. The tran sduction particles are non -replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of re porter molecules. The s ample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is rece ived. In some embodiments, a magnitude of the signal is in dependent from a quantity of the transduction particle above a predetermined quantity.

Method of determining the presence of an antibiotic resistant cell

Herein is described a method of determining the presence of an antibiotic resistant cell in a biological sample, comprising disposing the biological sample into a reaction chamber containing an antibiotic formulated to mix with the biological sample to form an assay media; mixing with the assay media a plurality of transduction particles associated with a cell phenotype, the plurality of transduction particles comprising a nucleic acid molecule for producing reporter molecules for generating a detectable signal, the antibiotic formulated to suppress production of reporter molecules; receiving a first signal indicating the presence of the antibiotic; maintaining the assay media and the plurality of transduction particles within the detection instrument to express the plurality of reporter molecules when the cell phenotype is present in the biological sample; and receiving a second signal indicating a quantity of the plurality of reporter molecules, thereby determining the presence of the antibiotic resistant cell.

Devices and methods for biological assay sample preparation and delivery

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/160838 Al 21 September 2017 (21.09.2017) WIPO I PCT (51) International Patent Classification: 62nd Street, Emeryville, CA 94608 (US). WALDEISEN, GO1N 1/28 (2006.01) John Robert; Diassess INC., 1412 62nd Street, Emeryville, CA 94608 (US). DIMOV, Ivan Krastev; Di-(21) International Application Number: PCT/U52017/022304 assess INC., 1412 62nd Street, Emeryville, CA 94608 (US). GRISWOLD, Ryan C.; Diassess INC., 1412 62nd (22) International Filing Date: Street, Emeryville, CA 94608 (US). RICHARDSON, 14 March 2017 (14.03.2017) Bruce; Diassess INC., 1412 62nd Street, Emeryville, CA 94608 (US). (25) Filing Language: English (74) Agents: STODDARD, Daniel et al.; Fenwick & West (26) Publication Language: English LLP, 801 California Street, Mountain View, CA 94041 (30) Priority Data: (US). 62/307,876 14 March 2016 (14.03.2016) US (81) Designated States (unless otherwise indicated, for every (71) Applicant: DIASSESS INC. [US/US]; 1412 62nd Street, kind of national protection available): AE, AG, AL, AM, Emeryville, CA 94608 (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (72) Inventors: MYERS, Frank B., III; Diassess INC., 1412 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, 62nd Street, Emeryville, CA 94608 (US). HO, Wei Hsu- HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, an; Diassess INC., 1412 62nd Street, Emeryville, CA KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 94608 (US). MITRA, Debkishore; Diassess INC., 1412 MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, [Continued on next pagel (54) Title: DEVICES AND METHODS FOR BIOLOGICAL ASSAY SAMPLE PREPARATION AND DELIVERY (57) Abstract: Devices and methods for preparing and delivering biolo-gical assay samples are provided herein. ...

Devices and methods for biological assay sample preparation and delivery

Devices and methods for preparing and delivering biological assay samples. The device comprising: a sample receiving module comprising a fluid container, a preparation solution, and a first attachment element; and a cap removably coupleable to the sample receiving module and further comprising: a pressurizing component and a second attachment element coupleable with the first attachment element; wherein the pressurizing component extends into and pressurizes the sample receiving module upon operative coupling of the first attachment element to the second attachment element. Alternatively the device comprising: a cap comprising a first chamber, a plunger comprising a piercing member, and a seal; and a sample receiving module which is coupleable to the cap and further comprises a second chamber, wherein when the sample receiving module is coupled to the cap, advancing the plunger pierces the seal with the piercing member and places the first chamber in fluidic communication with the second chamber. WO 2017/160838 PCT/US2017/022304 (~0 00 0N 00 0 0

Devices and methods for biological assay sample preparation and delivery

Devices and methods for preparing and delivering biological assay samples are provided herein. Components of such devices include a sample receiving module within which a biological assay sample can be prepared and a cap, which when operatively coupled with the sample receiving module, pressurizes the module. These devices can be employed for subsequently delivering a biological assay sample.

Devices and methods for biological assay sample preparation and delivery

Devices and methods for preparing and delivering biological assay samples are provided herein. Components of such devices include a sample receiving module within which a biological assay sample can be prepared and a cap, which when operatively coupled with the sample receiving module, pressurizes the module. These devices can be employed for subsequently delivering a biological assay sample.

Devices and methods for biological assay sample preparation and delivery

Devices and methods for preparing and delivering biological assay samples. The device comprising: a sample receiving module comprising a fluid container, a preparation solution, and a first attachment element; and a cap removably coupleable to the sample receiving module and further comprising: a pressurizing component and a second attachment element coupleable with the first attachment element; wherein the pressurizing component extends into and pressurizes the sample receiving module upon operative coupling of the first attachment element to the second attachment element. Alternatively the device comprising: a cap comprising a first chamber, a plunger comprising a piercing member, and a seal; and a sample receiving module which is coupleable to the cap and further comprises a second chamber, wherein when the sample receiving module is coupled to the cap, advancing the plunger pierces the seal with the piercing member and places the first chamber in fluidic communication with the second chamber. WO 2017/160838 PCT/US2017/022304 (~0 00 0N 00 0 0

Devices and methods for biological assay sample preparation and delivery

Devices and methods for preparing and delivering biological assay samples. The device comprising a sample receiving module comprising a first chamber for receiving a biological sample and a first attachment element and a cap removably coupleable to the sample receiving module. The cap comprises a second chamber containing a preparation solution and comprising a breakable seal. The cap further comprises a second attachment element operatively coupleable with the first attachment element. Operatively coupling the first attachment element with the second attachment element causes the breakable seal to break and place the first and second chambers in fluidic communication to create a prepared biological sample.

生物学的アッセイ試料調製および送り出しのための装置および方法

【課題】生物学的アッセイ試料を調製し、送り出すための装置および方法の提供。【解決手段】装置の部品は、生物学的アッセイ試料をその中で調製することができる試料受け入れモジュールと、試受け入れモジュールと動作的に結合されるときモジュールを加圧するキャップとを含む。これらの装置は、続いて生物学的アッセイ試料を送り出すために用いることができる。方法は、これらの装置のキャップを試料受け入れモジュールに動作的に結合する工程と、試料受け入れモジュールを加圧する工程とを含む。【選択図】図１

Systems and methods for detection of cells using engineered transduction particles

Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.