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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 4. Отображено 4.
04-07-2023 дата публикации

Functional marker and method for detecting rice GW3p6 gene by using melting curve

Номер: CN116377117A
Автор: YANG SHAOHUA, GUO XINRUI, CHEN ZAIJIE, LIU HUAQING, GUI YIJIE, CHEN RUI, ZHOU SHUFEN, WANG FENG
Принадлежит:

The invention discloses a functional marker and a method for detecting a rice GW3p6 gene by using a melting curve. A functional marker is designed by taking a functional site of a 15-base sequence 5 '-tccttcaccaagga-3' in a boundary region of a seventh intron and an eighth exon of a rice GW3p6 gene as a target, the 15-base sequence 5 '-tccttcaccaagga-3' is mutated into a sequence 5 '-agtatatacat-3' with 13 bases, and the functional marker is composed of a primer GW3p6-F and a primer GW3p6-R. According to the invention, two homozygous genotypes (wild type and mutant type) and heterozygous type of the rice gene GW3p6 can be accurately distinguished only by using a melting temperature analysis function of a conventional quantitative PCR instrument. Compared with the prior art, the detection method disclosed by the invention is low in cost, good in specificity, large in difference of melting temperatures of amplification products and capable of accurately distinguishing different genotypes.

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Номер записи: 1
01-12-2010 дата публикации

Primers for IMP molecular markers of tobacco

Номер: CN0101899436A
Автор: FAN LONGJIANG, XIAO BINGGUANG, GUI YIJIE, TONG ZHIJUN, LONGJIANG FAN, BINGGUANG XIAO, YIJIE GUI, ZHIJUN TONG
Принадлежит:

The invention relates to the field of plant genetic engineering, and aims to provide primers for IMP molecular markers of tobacco. The primers have nucleotide sequences shown as SEQ ID NO:1 to SEQ ID NO:10. The invention belongs to the technology of new biomolecular markers of the tobacco. The provided primers can be used for the IMP molecular markers of the tobacco. The fields for the IMP molecular markers mainly comprise (1) auxiliary selection breeding of the molecular markers of the tobacco, namely the selection breeding for tobacco varieties; (2) the construction of a genetic map of the tobacco; (3) the positioning and discovery of important functional genes of the tobacco; (4) the early diagnosis and screening of specific properties of the tobacco; and (5) the judgment of the genetic relationship of the tobacco (such as the mixing of the tobacco varieties, the analysis of the tobacco and wild species thereof and the like).

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Номер записи: 2
25-08-2010 дата публикации

Molecular marker method based on microRNA

Номер: CN0101812520A
Автор: LONGJIANG FAN, YIJIE GUI, BINGGUANG XIAO, FAN LONGJIANG, GUI YIJIE, XIAO BINGGUANG
Принадлежит:

The invention relates to the field of plant genetic engineering and aims to provide a molecular marker method based on microRNA (miRNA). The method comprises the following steps: (1) obtaining miRNA sequence data; (2) locating the miRNA sequences on a genome; (3) designing PCR primers; and (4) carrying out PCR amplification and detecting genetic polymorphism. The invention is new molecular marker technology and can be applied to animals and plants as well as microorganisms. The marker directly designs the primers based on the actually expressed miRNA sequences and is a functional marker. The marker is marker technology based on PCR technology and simultaneously can generate dozens of PCR product belts, thus being molecular marker technology with high throughput. The invention can be used in breeding through molecular marker-assisted selection, construction of genetic maps, location and discovery of important functional genes, early diagnosis and early screening of specific traits or diseases ...

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Номер записи: 3
25-04-2012 дата публикации

Molecular marker method based on microRNA

Номер: CN0101812520B
Автор: FAN LONGJIANG, GUI YIJIE, XIAO BINGGUANG, LONGJIANG FAN, YIJIE GUI, BINGGUANG XIAO
Принадлежит:

The invention relates to the field of plant genetic engineering and aims to provide a molecular marker method based on microRNA (miRNA). The method comprises the following steps: (1) obtaining miRNA sequence data; (2) locating the miRNA sequences on a genome; (3) designing PCR primers; and (4) carrying out PCR amplification and detecting genetic polymorphism. The invention is new molecular markertechnology and can be applied to animals and plants as well as microorganisms. The marker directly designs the primers based on the actually expressed miRNA sequences and is a functional marker. The marker is marker technology based on PCR technology and simultaneously can generate dozens of PCR product belts, thus being molecular marker technology with high throughput. The invention can be used in breeding through molecular marker-assisted selection, construction of genetic maps, location and discovery of important functional genes, early diagnosis and early screening of specific traits or diseases ...

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Номер записи: 4