Mulberry surface corrosion-causing antagonistic bacteria screening method

The invention discloses a mulberry surface corrosion-causing antagonistic bacteria screening method which is as follows: 1, taking clean and intact mulberry, chopping, then placing in sterile physiological saline, shaking mixed liquid for culturing at constant temperature to obtain antagonistic bacteria liquid; 2, diluting the antagonistic bacteria liquid with the sterile physiological saline to obtain diluted liquid, taking 1mL diluted liquid to applying to a PDA (potato dextrose agar) culture medium, and culturing at constant temperature to obtain bacterial colony group; and 3, picking the obtained bacterial colony group, inoculating onto the PDA culture medium for pure culture, and repeatedly separating for 3-5 times to obtain antagonistic bacteria single colony. The screening method has the advantages of simple operation, strong selectivity, and better separation effect.

Microbiological fermentation method for increasing DNJ (1-deoxynojirimycin) content in silkworm biomass

The invention relates to a microbiological fermentation method for increasing the DNJ (1-deoxynojirimycin) content in silkworm biomass. The method comprises the following steps: drying the silkworm biomass naturally or in hot air, grinding and screening for later use; activating ganoderma lucidum strain and culturing ganoderma lucidum liquid; weighing the silkworm biomass, glucose and KNO3 according to a ratio of the silkworm biomass to glucose to KNO3 of (4-5):(1.0-1.2):(0.8-1.0) respectively, dissolving in water of which the volume is 18-20 times the volume of the silkworm biomass, glucose and KNO3, and mixing uniformly; adjusting the pH value to neutrality, and sterilizing for 20-30 minutes at 120 DEG C; inoculating with the activated ganoderma lucidum liquid with volume ratio of the activated ganoderma lucidum liquid to silkworm biomass mixed liquor being 2-3%, and performing oscillating fermentation culture for 2-3 days by virtue of a shaker at room temperature; collecting the fermentation ...

Method for preparing silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide by ultrasonic-ionic liquid coupling technology

The invention discloses a method for preparing a silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide from by an ultrasonic-ionic liquid coupling technology. The method comprises the following steps: preparing silkworm chrysalis protein into a suspension by using distilled water, subsequently mixing the suspension with an isopyknic ionic liquid and adding into a flask, dipping an ultrasonic probe into a water phase so as to perform ultrasonic-ionic liquid coupling treatment, after the treatment is accomplished, centrifuging, collecting the silkworm chrysalis protein and recycling the ionic liquid, firstly washing the collected silkworm chrysalis protein and subsequently centrifuging so as to remove the residual ionic liquid, and preparing the ACE inhibitory peptide from pretreated silkworm chrysalis protein by using an enzyme method. Compared with the conventional untreated method, the method for preparing the silkworm chrysalis source ACE inhibitory peptide ...

Method for preparing hypotensive mulberry leaf tea bag

The invention provides a method for preparing a hypotensive mulberry leaf tea bag. The method comprises the following steps: removing stalks of fresh mulberry leaves, cleaning the fresh mulberry leaves, pulping and adding protease; performing ultrasonic assisted enzymolysis, and centrifuging after enzymolysis; concentrating supernatant of an enzymolysis product, and lyophilizing to obtain mulberry protein antihypertensive peptide; mixing and blending the mulberry protein antihypertensive peptide and mulberry leaf tea powder; packaging, and sterilizing to obtain the hypotensive mulberry leaf tea bag. The problem of mulberry leaf protein is difficult to hydrolyze due to the existence of pectin can be solved by utilizing ultrasonic wave application in the enzymolysis process, so that the ACE (angiotensin converting enzyme) inhibition activity of the mulberry protein enzymatic hydrolysate can be increased, and the IC 50 (half inhibit concentration) value is 1.12-2.76mg/mL.

Application of bacillus subtilis DC11 in increasing intestinal flora abundance of bombyx mori

The invention discloses application of bacillus subtilis DC11 in increasing intestinal flora abundance of bombyx mori. The bacillus subtilis DC11 disclosed by the invention is a dominant strain capable of tolerating the alkaline environment of bombyx mori intestinal tracts, can generate amylase, protease, lipase, cellulase and other digestive enzymes, and has great advantages in the aspects of promoting the growth performance of bombyx mori and improving the cut leaf conversion rate; when being applied to bombyx mori breeding, the bombyx mori strain can obviously improve the bombyx mori weight increase rate, promote the utilization of bombyx mori cellulose and increase of the spinning amount, has obvious inhibitory activity on common pathogenic bacteria such as staphylococcus aureus and escherichia coli, and has a great promotion effect on sericulture production.

Separating and purifying method of angiotensin converting enzyme inhibitor in ramulus mori skin

The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus mori skin fluid suspension, adding cellulase 3200-3800U/g, adjusting pH value to 4.5-5.0, carrying out supersonic wave cell wall breaking extraction, separation and filtering, and isolating supernatant to obtain ramulus mori skin leach liquid; 3, isolating anion resin 201X7 exchange column chromatography and sephadex G-25 from the ramulus mori skin leach liquid, and freeze drying the isolated products so as to obtain the angiotensin converting enzyme inhibitor in single component and high output; the method is simple in technology, and easy in industrialization scale production.

Mulberry powder health care tea and preparation method thereof

Leiocassis longirostris skin collagen antioxidation peptide, and preparation method and application thereof

The invention relates to a Leiocassis longirostris skin collagen antioxidation peptide, and a preparation method and application thereof. The preparation method comprises the following steps: removing alkali-soluble proteins from Leiocassis longirostris skin with an alkali solution, degreasing with n-hexane, extracting collagen with a lactic acid solution, dialyzing, carrying out freeze-drying to obtain the collagen, hydrolyzing the obtained collagen with proteinase under proper enzymolysis conditions, and carrying out freeze-drying on the enzymolysis product to obtain the Leiocassis longirostris skin collagen antioxidation peptide. The Leiocassis longirostris skin collagen antioxidation peptide has favorable activity, the IC50 for removing DPPH free radicals is 0.53-0.86 mg/ml, the IC50 for removing hydroxy free radicals is 0.72-0.91 mg/ml, and the IC50 for chelating Fe<2+> is 1.01-1.35 mg/ml.

Highly effective cultivating method for Chinese caterpillar fungus

Biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises

The invention discloses a biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises, which comprises the following steps: carrying out high-temperature sterilization on a certain amount of silkworm chrysalis powder, inoculating with a specific microorganism strain, carrying out solid fermentation, carrying out biological transformation processes of blanching, disinfection, degreasing and the like while soaking in ethanol, dehydrating, and crushing to obtain silkworm chrysalis protein powder with good color and no odor. The method has the advantage of convenient process, and is suitable for industrial processing production. The prepared silkworm chrysalis protein has no odor, and the color of the silkworm chrysalis powder is changed from puce to offwhite. The deodorized silkworm chrysalis protein has more reasonable amino acid composition, the content of seven essential amino acids is increased by 39-85%, and the content of the silkworm chrysalis ...

A method for preparing silkworm powder

Sustained-release pill of total alkaloids from strychnos and method for preparing same

The invention discloses a sustained-release pill of total alkaloids from strychnos and a method for preparing the same. The sustained-release pill of total alkaloids from strychnos has high bioavailability, controllable release and long pain-relieving time, is taken for fewer times, is safe and reliable and can not cause pollution when being produced. The strychnine and strychnine is used as the active component of the sustained-release pill of total alkaloids from strychnos and is mixed with hydrophilic matrix material and hydrophobic matrix material which are used as matrix to prepare the sustained-release pill of total alkaloids from strychnos. The sustained-release pill of total alkaloids from strychnos comprises the following components by weight percent: 1-8 percent of the strychnine, 80-88 percent of hydrophilic matrix material and 10-12 percent of hydrophobic matrix material, wherein the hydrophilic matrix material is the mixture of polyethylene glycol 4000 and polyethylene glycol ...

Method for screening rotten fungi on surface of mulberry

The invention discloses a method for screening rotten fungi on the surface of mulberry. The method comprises the following steps: (1) culturing the rotten fungi solution by utilizing rotten ripe mulberry; (2) preparing a primary screening culture medium by adopting pectin or sodium carboxymethylcellulose as an exclusive carbon source; (3) diluting the rotten fungi solution into different concentrations by utilizing bacteria-free normal saline, coating 1ml of dilute solution on the primary screening culture medium to be culture for 72h to 78h under the condition of 27 to 28 DEG C to obtain bacterial colony clusters; (4) purely culturing the bacterial colony growing on the primary screening culture medium in a PDA culture medium containing 10 percent of mulberry juice, continuously separating for 3 to 5 times to obtain a single colony, i.e. the mulberry rotten colony. The process is simple and convenient in operation, strong in selectivity and excellent in separation effect.

Highly effective cultivating method for Chinese caterpillar fungus

The invention discloses a highly effective culture method of Chinese caterpillar fungus. The method is that with the liquid fermentation of Chinese caterpillar fungus strain, a large number of Chinese caterpillar fungus mycelium are cultivated after a few days; the cultivated species of Chinese caterpillar fungus can be produced in a large scale; then the Chinese caterpillar fungus mycelium produced after fermentation are inoculated on silkworm chrysalis or rice medium which is treated by surface sterilization; the further cultivation of the Chinese caterpillar fungus mycelium are processed at a certain appropriate temperature and humidity with necessary ventilation and light management; a large amount of artificial cordyceps militaris which has same form and similar function with the wild Chinese caterpillar fungus is obtained after a few days. The highly effective culture method of Chinese caterpillar fungus of the invention has not only rapidness and effectiveness but also low culture ...

Method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides

The invention discloses a method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides. The method comprises the following steps: preparing channel catfish skin collagen into a solution with a substrate concentration of 1-3%, adjusting the pH value of the solution to 7.0-8.0; carrying out enzymolysis on the solution for 10-20 min at a temperature of 40-50 DEG C by using alpha-chymotrypsin, wherein the addition amount of the alpha-chymotrypsin is 1000-3000U/g; after the enzymolysis is completed, carrying out enzyme deactivation by using a boiling water bath; adjusting the pH value of reaction mixed liquid to 8.0-9.0; carrying out enzymolysis on the reaction mixed liquid for 20-30min at a temperature of 50-60 DEG C by using Alcalase enzyme, wherein the addition amount of the Alcalase enzyme is 2000-3000U/g; and after the enzymolysis is completed, carrying out enzyme deactivation by using the boiling water bath, and centrifugally collecting liquid ...