08-06-2017 дата публикации
Номер: US20170157291A1
Provided are transplants and methods for augmenting formation and restoration of organ and tissue, for example, bone formation, by administering autologous or allogeneic human embryonic-like adult stem cells (ELA cells). Also provided is a method for augmenting formation of tissues and organs by administering a transplant having ELA stem cells or combination of ELA stem cells. 1. A transplant composition comprising: a T-cell- or NK-cell-suppressive amount of a non-expanded population of human early lineage adult (ELA) stem cells , an immune cell population comprising plasmacytoid dendritic cells , and a cryopreservative , wherein the human ELA stem cells express at least one of Oct4 , Nanog and Sox2 , and do not detectably express CD13 , CD45 , CD90 or CD34 , and wherein the transplant composition is without detectable erythrocyte cells.2. The transplant composition of claim 1 , wherein the transplant is an allograft.3. The transplant composition of claim 2 , wherein the allograft is not MHC matched to the human transplant recipient.4. The transplant composition of claim 1 , wherein the transplant includes at least one component selected from the group consisting of plasma claim 1 , cell culture medium claim 1 , an antibacterial agent claim 1 , a growth factor claim 1 , a vitamin claim 1 , and a hormone.5. The transplant composition of claim 1 , further comprising a carrier having a matrix claim 1 , wherein the matrix conforms substantially to its insertion site and provides a structurally stable claim 1 , three dimensional surface that retains the transplant and supports ingrowth of ELA stem cells into the matrix at the insertion site.6. The transplant composition of claim 1 , wherein the transplant promotes the formation of a tissue selected from the group consisting of: connective tissue; bone; and dermal tissue.7. The transplant composition of claim 1 , wherein the human ELA stem cell numbers in the transplant comprise about 5000 to about 5×10human ELA stem ...
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