06-01-2022 дата публикации
Номер: US20220000793A1
A biomass-based encapsulating material for protection of probiotic activity and an encapsulating method are disclosed. The biomass-based encapsulating material includes a nano-film having two layers for encapsulating probiotics. A first layer is formed through natural biological macromolecules and metal ions on surfaces of the probiotics by covalent cross-linking or metal chelating action in situ, and a second layer is formed by interactions between a bio-enzyme and the natural biological macromolecules. The nano-film formed in situ on cell walls of probiotics is used for encapsulation and protection of probiotics. The nano-film is made of natural biomass, has low cost and has no toxic side effects. The relative activity of encapsulated probiotics is close to or higher than 90%, whether they are cultivated in environments under high concentration of antibiotics or active-free radicals. The relative activity of unprotected probiotics is below 30%, or even completely unable to survive. 1. A biomass-based encapsulating material for protection of probiotic activity , comprising a nano-film for encapsulating probiotics , the nano-film comprising two layers , wherein a first layer is formed through natural biological macromolecules and metal ions on surfaces of the probiotics by covalent cross-linking or metal chelating action in situ , and a second layer is formed by interactions between a bio-enzyme and the natural biological macromolecules.2. The biomass-based encapsulating material for protection of probiotic activity according to claim 1 , wherein the bio-enzyme is one of or a mixture of two or more of β-galactosidase claim 1 , amylopsin claim 1 , α-glucosaccharase claim 1 , β-glucosaccharase claim 1 , pancreatic lipase claim 1 , trypsin and cellulase at any proportion.3. The biomass-based encapsulating material for protection of probiotic activity according to claim 1 , wherein the metal ions are one of or a mixture of two or more of cations of Al claim 1 , Fe claim ...
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