28-03-2013 дата публикации
Номер: US20130078667A1
Принадлежит:
The present invention is directed towards methods of expanding circulating tumor cell populations and methods of detecting circulating tumor cells in a sample. 1. A method of expanding a circulating tumor cell population , comprising providing a cell population containing circulating tumor cells (CTCs) and culturing the cell population under conditions suitable for preferential expansion of the CTCs.2. The method of claim 1 , wherein cell population containing CTCs is obtained from a subject.3. The method of or claim 1 , wherein said cell population containing CTCs is obtained from a blood sample.4. The method of claim 3 , wherein said blood sample is peripheral blood.5. The method of claim 1 , further comprising obtaining a sample that contains a cell population containing CTC from a subject.6. The method of claim 5 , wherein said sample is a blood sample.7. The method of claim 6 , further comprising substantially removing red blood cells from the blood sample.8. The method of claim 7 , wherein red blood cells are removed using a method selected from the group consisting of fractionation claim 7 , red blood cell lysis claim 7 , cell sorting claim 7 , filtration claim 7 , adhesion claim 7 , density centrifugation or combinations thereof.9. The method of claim 1 , wherein the cell population containing CTCs is obtained from a cell culture.10. The method of any preceding claim claim 1 , wherein said cell population containing CTCs is cultured in a matrix.11. The method of claim 10 , wherein said matrix is solid or semisolid.129. The method of any one of - claims 1 , wherein said cell population containing CTCs is cultured in a spheroid culture.13. The method of claim 10 , wherein said matrix is selected from the group consisting of methylcellulose claim 10 , collagen claim 10 , and matrigel.14. The method of claim 13 , wherein the depth of said matrix is 0-1 cm.15. The method of claim 1 , wherein said cell population containing CTCs are cultured in media that contains ...
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