04-09-2003 дата публикации
Номер: US20030166259A1
Принадлежит:
Methods for accurately mixing two solutions, e.g., buffer and sample solutions, are provided. A method of mixing first and second fluids using first, second, and third chambers includes selecting a fluid mixture r, providing the first chamber with a first cross-sectional area A1, providing the third chamber with a second cross-sectional area A2, disposing the first fluid in the first chamber, disposing the second fluid in the second chamber, moving a first plunger within the first chamber at a speed S1towards a first port to dispense the first solution from the first chamber into the third chamber, and moving a second plunger substantially simultaneously with the first plunger within the third chamber at a speed S2away from the second port to dispense the second solution from the second chamber into the third chamber, wherein A2S2=A1S1(1+1/r). The sample and buffer can be mixed, in which case, the first, second, and third chambers can be buffer, sample collection, and mixing chambers, and ...
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