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Применить Всего найдено 2. Отображено 2.
02-03-2017 дата публикации

ORGANIC ACID SYNTHESIS FROM C1 SUBSTRATES

Номер: US20170058280A1
Автор: Calvin A. HENARD, Michael T. GUARNIERI, HENARD CALVIN A, GUARNIERI MICHAEL T, HENARD Calvin A., GUARNIERI Michael T.
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Presented herein are biocatalysts and methods for converting C1-containing materials to organic acids such as muconic acid or adipic acid. 1. An engineered cell , comprising at least one exogenously added gene encoding a 3-dehydroshikimate dehydratase , a protocatechuic acid decarboxylase , a catechol 1 ,2-dioxygenase , aroG , trpE , a phosphoenolpyruvate synthase , or a transketolase; wherein the cell is able to convert a C1 substrate to an organic acid.2. The engineered cell of claim 1 , wherein the cell comprises genes encoding a 3-dehydroshikimate dehydratase claim 1 , a protocatechuic acid decarboxylase claim 1 , and a catechol 1 claim 1 ,2-dioxygenase.3Klebsiella variicola.. The engineered cell of claim 2 , wherein the 3-dehydroshikimate dehydratase is AroZ from4Enterobacter cloacae.. The engineered cell of claim 2 , wherein the protocatechuic acid decarboxylase is AroY from5Acinetobacter.. The engineered cell of claim 2 , wherein the catechol 1 claim 2 ,2-dioxygenase is CatA from6Klebsiella variicola,Enterobacter cloacae,Acinetobacter.. The engineered cell of claim 2 , wherein the 3-dehydroshikimate dehydratase is AroZ from the protocatechuic acid decarboxylase is AroY from and the catechol 1 claim 2 ,2-dioxygenase is CatA from7. The engineered cell of claim 1 , wherein the cell further comprises an exogenously added gene encoding a phosphoketolase.8. The engineered cell of claim 7 , wherein the phosphoketolase is PktA or PktB.9. The engineered cell of claim 1 , wherein the cell further comprises an exogenously added gene encoding a CbbL claim 1 , CbbS claim 1 , CbbQ or CbbP polypeptide.10. The engineered cell of claim 9 , wherein the cell further comprises exogenously added genes encoding CbbL claim 9 , CbbS claim 9 , CbbQ and CbbP polypeptides.11. The engineered cell of claim 1 , wherein the cell is a C1 metabolizing cell.12Methylomonas, Methylobacter, Methylococcus, Methylosinus, Methylocystis, Methylomicrobium, Methanomonas, Methylophilus, Methylobacillus ...

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Номер записи: 1
05-03-2020 дата публикации

ORGANIC ACID SYNTHESIS FROM C1 SUBSTRATES

Номер: US20200071704A1
Автор: GUARNIERI Michael T., HENARD Calvin A.
Принадлежит:

Presented herein are biocatalysts and methods for converting C1-containing materials to organic acids such as muconic acid or adipic acid. 1. An engineered cell , comprising an exogenously added gene encoding a 3-dehydroshikimate dehydratase (AroZ) , a protocatechuic acid decarboxylase (AroY) , a catechol 1 ,2-dioxygenase (CatA) , a phspho-2-dehydro-3-deoxyheptonate aldolase (AroG) , an anthranilate synthase (TrpE) , a phosphoenolpyruvate synthase , or a transketolase; wherein the engineered cell is able to convert a C1 substrate to an organic acid.2. The engineered cell of claim 1 , wherein the cell comprises genes encoding a 3-dehydroshikimate dehydratase (AroZ) claim 1 , a protocatechuic acid decarboxylase (AroY) claim 1 , and a catechol 1 claim 1 ,2-dioxygenase (CatA).3Klebsiella. The engineered cell of claim 2 , wherein the 3-dehydroshikimate dehydratase (AroZ) is from variicola.4Enterobacter cloacae.. The engineered cell of claim 2 , wherein the protocatechuic acid decarboxylase (AroY) is from5Acinetobacter.. The engineered cell of claim 2 , wherein the catechol 1 claim 2 ,2-dioxygenase (CatA) is from6KlebsiellaEnterobacter cloacaeAcinetobacter.. The engineered cell of claim 2 , wherein the 3-dehydroshikimate dehydratase (AroZ) is from variicola claim 2 , the protocatechuic acid decarboxylase (AroY) is from claim 2 , and the catechol 1 claim 2 ,2-dioxygenase (CatA) is from7. The engineered cell of claim 1 , wherein the cell further comprises an exogenously added gene encoding a phosphoketolase.8. The engineered cell of claim 7 , wherein the phosphoketolase is PktA or PktB.9. The engineered cell of claim 1 , wherein the cell further comprises an exogenously added gene encoding a CbbL claim 1 , CbbS claim 1 , CbbQ or CbbP polypeptide.10. The engineered cell of claim 9 , wherein the cell further comprises exogenously added genes encoding CbbL claim 9 , CbbS claim 9 , CbbQ and CbbP polypeptides.11. The engineered cell of claim 1 , wherein the engineered cell is a ...

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Номер записи: 2