20-09-2012 дата публикации
Номер: US20120237946A1
Demyelinated axons were remyelinated in the demyelinated rat model by collecting bone marrow cells from mouse bone marrow and transplanting the mononuclear cell fraction separated from these bone marrow cells. 1diluting marrow cells or cord blood cells;isolating a mononuclear cell fraction; andselecting cells having surface markers SH2(+), SH3(+), SH4(+), CD29(+), CD44(+), CD14(−), CD34(−), and CD45(−) from the mononuclear cell fraction,wherein said selected cells are capable of differentiating into neural cells or glia cells.. A method for preparing a mononuclear cell fraction, comprising: This application is a Divisional of U.S. patent application Ser. No. 13/040,954, filed Mar. 4, 2011, which is a Continuation of application Ser. No. 12/076,092, filed Mar. 13, 2008, which is a Continuation of application Ser. No. 11/189,050, filed Jul. 26, 2005, now abandoned, which is a Divisional of application Ser. No. 10/330,963, filed Dec. 23, 2002, now U.S. Pat. No. 7,098,027, which is a Continuation-In-Part of PCT/JP01/05456, filed Jun. 26, 2001, the contents of all of which are incorporated herein by reference in their entirety.The present invention relates to cells derived from bone marrow cells, cord blood cells, or embryonic hepatic tissues that can differentiate into neural cells, and cell fractions containing such cells. It is expected that these cells and cell fractions can be used to treat neurological diseases, particularly in autologous transplantation therapy.Transplantation of oligodendrocytes (i.e., oligodendroglia) (Archer D. R., et al., 1994. Exp. Neurol. 125:268-77; Blakemore W. F., Crang A. J., 1988. Dev. Neurosci. 10:1-11; Gumpel M., et al. 1987. Ann. New York Acad. Sci. 495:71-85) or myelin-forming cells, such as Schwann cells (Blakemore W. F., 1977. Nature 266:68-9; Blakemore W. F., Crang A. J., 1988. Dev. Neurosci. 10:1-11; Honmou O. et al., 1996. J. Neurosci. 16:3199-208), or olfactory ensheating cells (Franklin R. J. et al., 1996. Glia 17:217-24; ...
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