SYBR GreenI real-time quantitative polymerase chain reaction (PCR) detection method for S gene of transmissible gastroenteritis virus of swine

The invention discloses an SYBR GreenI real-time quantitative PCR detection method for the S gene of transmissible gastroenteritis virus of swine, which comprises the following steps: extracting total RNA of viral gastroenteritis; designing primers; performing reverse transcription; performing PCR amplification of a fragment of the S gene of transmissible gastroenteritis virus of swine; cloning and identifying the S gene of transmissible gastroenteritis virus of swine; preparing a standard product template; performing fluorescence quantitative PCR amplification; obtaining a kinetic curve and a standard curve; and performing repeatability, sensibility and specificity tests. In the method of the invention, only a good S type amplification curve is shown, only specific single peak of the product of the amplification appears, and the Tm value of the product is uniform; the detection sensitivity reaches 3 copies/microliter, and the sensitivity is high; the method avoids cross reaction with other ...