METHODS AND SYSTEMS FOR DETERMINING PLATELET CONCENTRATION

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system () and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell (); analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions. 169-. (canceled)70. A method for determining a platelet concentration in a blood sample comprising:mixing a first aliquot of the blood sample with a diluent to form a first suspension;mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells to form a second suspension;measuring DC impedance signals of the first suspension passing through an aperture;measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell;analyzing the DC impedance signals of the first suspension to obtain a first platelet distribution;analyzing the light scatter signals and the fluorescent signals of the second suspension to differentiate platelets from white blood cells and/or nucleated red blood cells and to obtain a second platelet distribution; anddetermining the platelet concentration of the ...

Blood cell analysis method and blood cell analyzer

A blood cell analysis method and a blood cell analyzer are provided. In the method and analyzer, characteristic information of white blood cell fragments is obtained based on side scattered light information and fluorescence information, characteristic information of platelets is obtained based on forward scattered light information and fluorescence information and then a count value for the platelets is acquired based on the characteristic information of the platelets and the characteristic information of the white blood cell fragments. The present invention can avoid the influence of the white blood cell fragments on the platelet counting, thereby ensuring the accuracy of the platelet counting without increasing costs.

Methods and systems for determining platelet concentration

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system (100) and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell (20); analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.

Methods and systems for determining platelet concentration

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell; analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.

Method and system for determining platelet concentration

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system (100) and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell (20); analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.

Method and system for determining platelet concentration

Methods and systems for determining platelet concentration

Disclosed are a method for determining a platelet concentration of a blood sample, a hematology system and a storage medium. The method for determining a platelet concentration in a blood sample includes: forming a first suspension by mixing a first aliquot of the blood sample with a diluent; forming a second suspension by mixing a second aliquot of the blood sample with a lytic agent and a fluorescent dye to lyse red blood cells and stain white blood cells; measuring DC impedance signals of the first suspension passing through an aperture; measuring light scatter signals and fluorescent signals of the second suspension passing through an optical flow cell; analyzing DC impedance signals of the first suspension to obtain a first platelet distribution; analyzing light scatter signals and fluorescent signals of the second suspension to differentiate platelets from white blood cells and to obtain a second platelet distribution; and determining platelet data, such as the platelet concentration of the blood sample using the first and second platelet distributions.

Method and system for determining platelet concentration

Method and system for analysing a blood sample

Disclosed are a method for analyzing a blood sample and a hematology system which includes acquiring DC impedance signals of a first suspension from the blood sample, acquiring at least two type of optical signals of a second suspension from the blood sample, wherein the at least two type of optical signals comprise forward angle light scatter signals and first optical signals for providing cellular content information, acquiring a first platelet distribution using the DC impedance signals, acquiring a second platelet distribution using the at least two type of optical signals, and acquiring a third platelet distribution using the first platelet distribution and the second platelet distribution, wherein the first suspension is formed by mixing a first aliquot of the blood sample with a diluent, and the second suspension is formed by mixing a second aliquot of the blood sample with a treatment reagent, which comprises a lytic agent to lyse red blood cells in the second aliquot of the blood sample.

Method and system for analysing a blood sample

Disclosed are a method for analyzing a blood sample and a hematology system which includes acquiring DC impedance signals of a first suspension from the blood sample, acquiring at least two type of optical signals of a second suspension from the blood sample, wherein the at least two type of optical signals comprise forward angle light scatter signals and first optical signals for providing cellular content information, acquiring a first platelet distribution using the DC impedance signals, acquiring a second platelet distribution using the at least two type of optical signals, and acquiring a third platelet distribution using the first platelet distribution and the second platelet distribution, wherein the first suspension is formed by mixing a first aliquot of the blood sample with a diluent, and the second suspension is formed by mixing a second aliquot of the blood sample with a treatment reagent, which comprises a lytic agent to lyse red blood cells in the second aliquot of the blood sample.

Blood cell analysis method and blood cell analyzer

A blood cell analysis method and a blood cell analyzer are provided. In the method and analyzer, characteristic information of white blood cell fragments is obtained based on side scattered light information and fluorescence information, characteristic information of platelets is obtained based on forward scattered light information and fluorescence information and then a count value for the platelets is acquired based on the characteristic information of the platelets and the characteristic information of the white blood cell fragments. The present invention can avoid the influence of the white blood cell fragments on the platelet counting, thereby ensuring the accuracy of the platelet counting without increasing costs.