08-08-2023 дата публикации
Номер: CN116549479A
Принадлежит:
The invention discloses an application of PYCR1 siRNA in enhancing the sensitivity of lung cancer cells to TRAIL and a method, an RNAi technology is adopted, the expression quantity of a PYCR1 gene is remarkably inhibited, PYCR1 siRNA1 with the optimal inhibition effect is obtained, the PYCR1 siRNA1 is transfected into the lung cancer cells NCI-H1299, analysis finds that the sensitivity of the lung cancer cells to TRAIL is reduced to 50 ng/mL from 400 ng/mL, the sensitivity is remarkably improved, and the application of the PYCR1 siRNA in enhancing the sensitivity of the lung cancer cells to TRAIL is achieved. And a strategy and a method capable of remarkably enhancing the treatment effect of the TRAIL on the lung cancer patient are established. The PYCR1 siRNA and the TRAIL are used in a combined manner, so that the curative effect of the TRAIL can be improved, the metering dependence can be reduced, and meanwhile, a good effect can also be achieved for patients relatively insensitive ...
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