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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 4. Отображено 4.
01-09-2010 дата публикации

Method for separating and culturing mouse primitive spermatogonia

Номер: CN0101818127A
Автор: HONGGUO CAO, JIANPING DING, FUGUI FANG, YUNSHENG LI, XUGUANG LIU, YA LIU, CHUNHUAN REN, RUI SONG, YONG TAO, HUIQUN YIN, YUNHAI ZHANG, ZIJUN ZHANG, XIAORONG ZHANG, CAO HONGGUO, DING JIANPING, FANG FUGUI, LI YUNSHENG, LIU XUGUANG, LIU YA, REN CHUNHUAN, SONG RUI, TAO YONG, YIN HUIQUN, ZHANG YUNHAI, ZHANG ZIJUN, ZHANG XIAORONG
Принадлежит:

The invention discloses a method for separating and culturing mouse primitive spermatogonia. In the method, the mouse primitive spermatogonia are separated by utilizing the characteristics of neutral protease, such as mild digestibility performance, high efficiency and small stimulation to cells; the step of enrichment and subculture of the mouse primitive spermatogonia are organically combined; and adherent mouse primitive spermatogonium colony is digested by utilizing the neutral protease, so a better enrichment effect is achieved. The method for separating and culturing the mouse primitive spermatogonia is characterized by simple separating and culturing operation, short period, good effect and low cost.

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Номер записи: 1
07-09-2011 дата публикации

Porcine somatic cell mutagensis method

Номер: CN0102174466A
Автор: CAO HONGGUO, ZHANG XIAORONG, LIU YA, ZHANG YUNHAI, TAO YONG, FANG FUGUI, YIN HUIQUN, SUN XUEPING, XUE YIJIE, ZHANG WEIQIN, HUANG WEILING, CHEN TAO, LI YUNSHENG, REN CHUNHUAN, ZHANG ZIJUN, DING JIANPING, LIU HONGYU, LIU XUGUANG, WANG LISHENG, HONGGUO CAO, XIAORONG ZHANG, YA LIU, YUNHAI ZHANG, YONG TAO, FUGUI FANG, HUIQUN YIN, XUEPING SUN, YIJIE XUE, WEIQIN ZHANG, WEILING HUANG, TAO CHEN, YUNSHENG LI, CHUNHUAN REN, ZIJUN ZHANG, JIANPING DING, HONGYU LIU, XUGUANG LIU, LISHENG WANG
Принадлежит:

The invention discloses a porcine somatic cell mutagensis method. Porcine embryonic fibroblasts are infected and acted a plurality of times by using slow viruses carrying enhanced green fluorescent protein markers; the original fibrous growth forms of the porcine embryonic fibroblasts are changed step by step under a growing environment condition of a stem cell culture solution to form nest-like cell colonies; separated, cultured and sub-cultured cell colonies have clear edges and boundaries, stable growth states and normal karyotypes; alkaline phosphatase is expressed to be positive; immunocytochemical detection shows that Oct4, Nanog and SSEA-1 proteins are expressed to be positive; teratoma comprising three embryonic layers is formed by differentiation in vivo; and the result proves that the mutagenic porcine somatic cells have the stem cell properties under the action of the slow viruses.

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Номер записи: 2
26-01-2011 дата публикации

Method for inducing porcine induced pluripotent stem cells

Номер: CN0101955910A
Автор: YUNHAI ZHANG, HONGGUO CAO, XIAORONG ZHANG, YUNSHENG LI, HUIQUN YIN, XUEPING SUN, YIJIE XUE, WEIQIN ZHANG, WEILING HUANG, TAO CHEN, RUI SONG, YONG TAO, YA LIU, FUGUI FANG, XUGUANG LIU, CHUNHUAN REN, ZIJUN ZHANG, ZONGJUN YIN, JIANPING DING, ZHANG YUNHAI, CAO HONGGUO, ZHANG XIAORONG, LI YUNSHENG, YIN HUIQUN, SUN XUEPING, XUE YIJIE, ZHANG WEIQIN, HUANG WEILING, CHEN TAO, SONG RUI, TAO YONG, LIU YA, FANG FUGUI, LIU XUGUANG, REN CHUNHUAN, ZHANG ZIJUN, YIN ZONGJUN, DING JIANPING
Принадлежит:

The invention discloses a method for inducing porcine induced pluripotent stem cells, which comprises the following steps of: constructing a fusion protein lentivirus expression vector for inducing the porcine induced pluripotent stem cells by using an exogenous defined factor and an enhanced green fluorescent protein (EGFP) reporter gene; subculturing porcine embryo fibroblasts for expressing exogenous defined factor fusion protein; and separating and culturing cell clones with clear colony edges step by step to obtain the porcine induced pluripotent stem cells, wherein the growth state of cell colonies is stable, karyotype is normal, alkaline phosphatase test is positive, and immunocytochemical detection shows that the expression of Oct4, Nanog and SSEA-1 protein is positive, and teratomas with three embryonic layers can be formed by differentiation in vivo.

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Номер записи: 3
02-11-2011 дата публикации

Method for inducing bovine induced pluripotent stem cells

Номер: CN0102229909A
Автор: HONGGUO CAO, XIAORONG ZHANG, YUNHAI ZHANG, YUNSHENG LI, HUIQUN YIN, YONG TAO, YA LIU, FUGUI FANG, XUGUANG LIU, CHUNHUAN REN, ZIJUN ZHANG, JIANPING DING, HONGYU LIU, LISHENG WANG, CAO HONGGUO, ZHANG XIAORONG, ZHANG YUNHAI, LI YUNSHENG, YIN HUIQUN, TAO YONG, LIU YA, FANG FUGUI, LIU XUGUANG, REN CHUNHUAN, ZHANG ZIJUN, DING JIANPING, LIU HONGYU, WANG LISHENG
Принадлежит:

The invention discloses a method for inducing bovine induced pluripotent stem cells, which comprises: constructing a fusion protein lentivirus expression vector for inducing bovine induced pluripotent stem cells by using an exogenous defined factor and enhanced green fluorescent protein (EGPF) reporter gene; cultivating and passing bovine fetus fibroblast cells in which the fusion protein of the exogenous defined factor is expressed; gradually separating and cultivating cell colonies with clearly defined colony borders; and obtaining the bovine induced pluripotent stem cells, if the growth states of the cell colonies are stable, the karyotype is normal, the cell colonies are negative in alkaline phosphatase test, the expression of proteins Oct4, Nanog and SSEA-1 is positive in immunocyte chemical tests, teratoma having three embryonic layers can be differentiated in vivo, and the result proves the separated cell conolnies have the characteristics of embryonic stem cells.

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Номер записи: 4