C-TERMINAL MODIFICATION OF POLYPEPTIDES

The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaa-Xaa-His, wherein Xaais L, Y or F and Xaais R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin. 1. A mutated trypsin comprising an amino acid substitution at position K60 and at position D189 , and an amino acid substitution by histidine at position N143 or position E151.2. The mutated trypsin of wherein K60 is substituted by E or D.3. The mutated trypsin of wherein D189 is substituted by K claim 1 , H or R.4. (canceled)5. A method of producing a C-terminally transacylated target peptide comprising the steps of:{'sub': 1', '2', '1', '2', '1, 'providing a polypeptide comprising a target peptide and a restriction site peptide comprising the cleavage site Xaa-Xaa-His, wherein Xaais L, Y or F, and Xaais R or K, wherein said restriction site peptide overlaps with the target peptide by the amino acid Xaaat the C-terminal end of said target peptide,'}{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'sub': '1', 'bringing said peptide into contact with a trypsin mutant according to under conditions allowing for endoproteolytic cleavage after Xaa, thereby forming an endoprotease target peptide peptide-acyl-intermediate,'}adding an appropriate nucleophile, and,upon nucleophilic attack and binding of said nucleophile to the C-terminus of the target peptide, releasing the mutated trypsin from the endoprotease target peptide-acyl-intermediate.6. The method of wherein said nucleophile is selected from the group consisting of a primary amine group claim 5 , an imine group claim 5 , a secondary amine group claim 5 , a thiol group and ...

USE OF A BIS-MALEIC ANHYDRIDE CROSS-LINKING AGENT FOR FIXATION OF A CELL OR TISSUE SAMPLE

The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like. 2. The kit according to claim 1 , wherein the linker X includes at least one bond selected from the group consisting of alkyls claim 1 , esters claim 1 , ethers claim 1 , amides claim 1 , and thios.3. The kit according to claim 1 , wherein the physiological buffer includes a physiological concentration of salt.4. The kit according to claim 1 , further including at least one antibody.5. The kit according to claim 4 , further including at least one nucleic acid.6. The kit according to claim 5 , wherein the nucleic acid is a primer suitable for use with a PCR based assay.7. The kit according to claim 1 , further including at least one nucleic acid.8. The kit according to claim 7 , wherein the nucleic acid is a primer suitable for use with a PCR based assay. This application is a divisional application of U.S. patent application Ser. No. 13/400,862 filed on Feb. 21, 2012, which is itself a continuation of International Application No. PCT/EP2010/005089, filed Aug. 19, 2010, which claims the benefit of European Patent Application No. 09010767.3, filed Aug. 21, 2009, the disclosures of which are hereby incorporated by reference in their entirety.The instant application includes a Sequence Listing which has been submitted in ASCII format via EFS-Web and is ...

Compounds with a branched linker

The present invention concerns new compounds comprising a branched linker and their use for producing conjugates for applications in diagnostic or therapeutic methods.

Use of a bis-maleic anhydride cross-linking agent for fixation of a cell or tissue sample

The present disclosure relates to novel bis-maleic anhydrides and to the surprising discovery that bis-maleic anhydride cross-linking agents can be used for preservation/fixation of a cell or tissue sample. Various bis-maleic anhydride cross-linking agent scan be used in methods requiring fixation of a cell or tissue sample. These reagents and methods are especially useful in procedures that require that the fixation agent be removed in order to facilitate analysis with other reagents. The inventive reagents and methods make it easier to reliably assay for various proteins, a nucleic acid and the like using analytical methods such as like immunohistochemistry, fluorescence in situ hybridization, RT-PCR, and the like.

Conjugates of defined stoichiometry

The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 5 kD 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomolecule-linker product of uniform stoichiometry in a pre-selected amount.

Modular microelectronic-system for use in wearable electronics

The invention relates to a modular microelectronic-system comprising: a first connection module ( 12 ) which can be firmly connected to user interface devices integrated textile material; and a second connection module ( 14 ) which comprises user interface devices ( 24, 26 ) and/or which an be connected to an external device; in addition to an electronic module ( 10 ) which can be connected to the first connection module ( 12 ) or to the second connection module ( 14 ).

Modularl microelectronic-system for use in wearable electronics

The invention relates to a modular microelectronic-system comprising: a first connection module (12) which can be firmly connected to user interface devices integrated into textile material; and a second connection module (14) which comprises user interface devices (24, 26) and/or which an be connected to an external device; in addition to an electronic module (10) which can be connected to the first connection module (12) or to the second connection module (14).

Oligomeric carrier molecules with defined marker groups and haptens

MODULAR AUFGEBAUTES MIKROELEKTRONIK-SYSTEM ZUM GEBRAUCH IN ßWEARABLE ELECTRONICSß

Textilgewebestruktur, flächenverkleidungsstruktur und verfahren zum bestimmen eines abstands von mikroelektronikelementen der textilgewebestruktur zu mindestens einer referenzposition

Textilgewebestruktur, Flächenverkleidungsstruktur und Verfahren zum Bestimmen eines Abstands von Mikroelektronikkomponenten der Textilgewebestruktur zu mindestens einer Referenzposition Eine Textilgewebestruktur weist eine Mehrzahl von Mikroelektronikkomponenten, welche in der Textilgewebestruktur angeordnet sind, elektrisch leitfähige Fäden, welche die Mehrzahl von Mikroelektronikkomponentenmiteinander koppeln, leitfähige Datenübertragungs-Fäden, welche die Mehrzahl von Mikroelektronikkomponentenmiteinander koppeln, und elektrisch nicht-leitfähigen Fäden auf. Ferner sind die leitfähigen Fäden und die leitfähigen Datenübertragungs-Fäden am Rande der Textilgewebestruktur jeweils mit elektrischen Schnittstellen bzw. Datenübertragungs-Schnittstellen versehen.