SAMPLE COLLECTION DEVICES WITH BLOOD STABILIZING AGENTS

Disclosed are devices for collecting and stabilizing blood or plasma and which contain an anti-coagulant, an antiplatelet agent, and a solubilization agent, and which may optionally include at least one other blood stabilization agent. Methods of making and using the devices in clinical medicine are also provided. 1. A device for collecting and stabilizing blood or plasma , comprising a first end and a second end and at least one interior wall defining a reservoir portion for receiving whole blood or plasma , and which comprises an anticoagulant , an antiplatelet agent comprising a prostaglandin , a phosphodiesterase inhibitor , a cyclooxygenase inhibitor , or a combination or two or more thereof , and a solubilization agent , wherein the anticoagulant and the antiplatelet agent are each present in an amount to stabilize the blood or plasma.2. The device of claim 1 , wherein the anticoagulant is selected from the group consisting of EDTA or a salt thereof claim 1 , oxalates claim 1 , citrate claim 1 , heparin claim 1 , a combination of citrate claim 1 , theophylline claim 1 , adenosine and dipyridamole (CTAD) claim 1 , sodium polyanethol sulfonate claim 1 , acid citrate dextrose claim 1 , and combinations of two or more thereof.3. The device of claim 1 , wherein the anticoagulant is present in a concentration of about 1 mM to about 200 mM claim 1 , relative to volume of the blood or plasma collected into the device.4. The device of claim 1 , wherein the antiplatelet agent is a prostaglandin.5. The device of claim 4 , wherein the prostaglandin comprises prostaglandin E1 claim 4 , prostaglandin E2 or a combination thereof.6. The device of claim 1 , wherein the prostacyclin comprises carbaprostacyclin claim 1 , beraprost claim 1 , iloprost claim 1 , 5 claim 1 ,6-dihydroprostacyclin claim 1 , ciprostene claim 1 , limaprost claim 1 , 13 claim 1 ,14-dehydro-15-cyclohexyl carbaprostacyclin claim 1 , taprostene claim 1 , and or a combination of two or more thereof.7. The ...

METHODS AND KITS FOR THE DIAGNOSIS OF INFLUENZA

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug. 1. A method for detecting an influenza virus comprising:(a) contacting a sample with an immunoassay buffer adapted for an immunoassay for detecting an influenza virus type A or an influenza virus type B, thereby obtaining an immunoassay test sample;(b) contacting a first portion of the immunoassay test sample to a test comprising an immunoassay for detecting an influenza type A or type B, thereby detecting the presence or absence of influenza type A or type B in the sample;(c) contacting a second portion of the immunoassay test sample with a matrix comprising a neuraminidase assay buffer, thereby obtaining a neuraminidase test sample; and(d) contacting the neuraminidase test sample with a neuraminidase assay, thereby detecting the presence of a neuraminidase in the sample.2. The method of claim 1 , wherein the immunoassay buffer is incompatible with an assay for neuraminidase activity.32. The method of any one of - claims 1 , wherein the immunoassay buffer inhibits neuraminidase activity.43. The method of any one of - claims 1 , wherein steps (c) and (d) are performed in the same vessel.5. The method of claim 4 , wherein the vessel comprises a plurality of chambers.65. The method of any one of - claims 4 , wherein the vessel comprises a chamber comprising the matrix claims 4 , and a chamber comprising reagents for the neuraminidase assay.76. The method of any one of - claims 1 , wherein the matrix comprises a cross-linked polysaccharide.86. The method of any one of - claims 1 , wherein the matrix is selected from the group consisting of sephadex and sepharose.98. The method of any one of - claims 4 , wherein the immunoassay test sample and the neuraminidase test sample are moved in the vessel by one or more forces ...

METHODS AND KITS FOR THE DIAGNOSIS OF INFLUENZA

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug. 18.-. (canceled)9. A method for detecting an influenza virus comprising:(a) contacting a sample with an immunoassay buffer, wherein the immunoassay buffer inhibits neuraminidase activity and is adapted for an immunoassay for detecting an influenza virus type A or an influenza virus type B, thereby obtaining an immunoassay test sample;(b) contacting a first portion of the immunoassay test sample to a test comprising an immunoassay for detecting an influenza type A or type B, thereby detecting the presence or absence of influenza type A or type B in the sample;(c) contacting a second portion of the immunoassay test sample with a matrix comprising a neuraminidase assay buffer, thereby obtaining a neuraminidase test sample; and(d) contacting the neuraminidase test sample with a neuraminidase assay, wherein the neuraminidase assay comprises a reagent selected from the group consisting of 1,1,1-Trifluro-4-(4-hydroxyphenyl)butan-2-one (para), 1, 1,1-Trifluro-4-(3-hydroxyphenyl)butan-2-one (meta), and 1,1,1-Trifluro-4-(2-hydroxyphenyl)butan-2-one (ortho), thereby detecting the presence of a neuraminidase in the sample. This application is a continuation of U.S. Ser. No. 16/384,020 filed Apr. 15, 2019 which is a division of U.S. application Ser. No. 14/781,008 filed Sep. 28, 2015, now U.S. patent Ser. No. 10/317,404 issued Jun. 11, 2019 which is the U.S. National Phase of Application No. PCT/US2014/032567 entitled “METHODS AND KITS FOR THE DIAGNOSIS OF INFLUENZA” filed Apr. 1, 2014 and published in English on Oct. 9, 2014 as WO2014/165536 which claims the benefit of U.S. Provisional Application No. 61/807,185 filed Apr. 1, 2013 entitled “Point Of Care (POC) Influenza Antiviral Susceptibility Test” each of which is incorporated ...

Methods and kits for the diagnosis of influenza

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug.

Methods and kits for the diagnosis of influenza

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an anti-viral drug.

User activated iontophoretic device and method for using same

The iontophoretic device or patch (10) of the present invention includes an electrode assembly (12), having at least one electrode, an electrode reservoir (14) and at least one drug reservoir (l6), which are held or contained within a pouch or other suitable structure (18). It should be appreciated that a return electrode may be combined in the assembly (12) or separately provided as is well known in the art. The device is divided or otherwise separated into two portions, one portion (20) (first) includes the electrode assembly (12) and the electrode reservoir (14) with the electrode reservoir being situated adjacent to the electrode assembly and holding an electrolyte (26). The other portion (22) (second) includes the drug reservoir (16) which holds the medication or drug (28), preferably in an ionized or ionizable form, to be delivered iontophoretically.

Substrate composition for alkaline phosphatase and method¹for assay using same

A composition contains levamisole and a substrate for alkaline phosphatase in a high pH buffer including 2-amino-2-methyl-1-propanol. The composition may be included in a kit of materials useful in performing an immunoassay in which intestinal alkaline phosphatase is the label. The invention includes a method for detection of a viral antigen by an immunoassay including intestinal alkaline phosphatase as the label and the composition of the invention to provide stabilized levamisole to inhibit nonintestinal alkaline phosphatase.

User activated iontophoretic device and method for using same

The iontophoretic device or patch (10) of the present invention includes an electrode assembly (12), having at least one electrode, an electrode reservoir (14) and at least one drug reservoir (16), which are held or contained within a pouch or other suitable structure (18). It should be appreciated that a return electrode may be combined in the assembly (12) or separately provided as is well known in the art. The device is divided or otherwise separated into two portions, one portion (20) (first) includes the electrode assembly (12) and the electrode reservoir (14) with the electrode reservoir being situated adjacent to the electrode assembly and holding an electrolyte (26). The other portion (22) (second) includes the drug reservoir (16) which holds the medication or drug (28), preferably in an ionized or ionizable form, to be delivered iontophoretically.

Substrate composition for alkaline phosphatase and method for assay using same.

Fluorogenic and chromogenic β-lactamase substrates

Chromogenic and fluorogenic substrates for β-lactamase, methods for synthesis thereof and methods for detecting β-lactamase in a sample are provided. The substrates are substantially colorless or substantially nonfluorescent β-lactam compounds which include an electronegative leaving group. The leaving group comprises a carbamate, carbonate, thiocarbamate or thiocarbonate linkage and a fluorescent moiety or a moiety capable of producing a visually detectable colored product. Upon cleavage of the lactam ring by β-lactamase, the leaving group is liberated and fluorescence or a colored product is produced.

Immunoassay on a preblocked solid surface

Method and kit for influenza diagnosis

【課題】１つには、有害事象を回避し、薬物耐性の進化を遅らせ、かつ患者及び医療制度を省力化するために特異性株の診断を提供しうる、ポイントオブケア検査を提供すること。【解決手段】本明細書で提供されるいくつかの実施形態は、結合アッセイに関する。いくつかの実施形態において、インフルエンザA型又はインフルエンザB型を同定するためのアッセイは、抗ウイルス薬に対するインフルエンザノイラミニダーゼの感受性を決定するためのアッセイと結合される。【選択図】図８

Methods and kits for the diagnosis of influenza

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug.

Material packaging and method for the determination of the analyte

Immunoassay on a preblocked solid surface.

User activated iontophoretic device and method for using same.

Dispositif ou plaque (10) d'ionophorèse comprenant un ensemble électrode (12) constitué au minimum d'une électrode, d'un réservoir d'électrode (14) et d'un réservoir à médicaments (16), logés à l'intérieur d'une poche ou de tout autre élément approprié (18). Il est souhaitable qu'une électrode de retour puisse être associée à l'ensemble (12) ou soit prévue séparément, selon les modalités connues de la technique. Le dispositif est divisé ou bien séparé en deux parties, l'une (20) (la première) comprenant l'ensemble électrode (12) et le réservoir à électrode (14), ce dernier étant contigu à l'ensemble électrode et renfermant un électrolyte (26). L'autre partie (22) (la seconde) comprend le réservoir à médicaments (16) qui renferme la médication (28), de préférence sous forme ionisée ou ionisable, devant être administrée par ionophorèse. Iontophoresis device or plate (10) comprising an electrode assembly (12) consisting at least of an electrode, an electrode reservoir (14) and a medication reservoir (16), housed inside a pocket or any other suitable item (18). It is desirable that a return electrode can be associated with the assembly (12) or be provided separately, according to methods known in the art. The device is divided or separated into two parts, one (20) (the first) comprising the electrode assembly (12) and the electrode reservoir (14), the latter being contiguous to the electrode assembly and containing a electrolyte (26). The other part (22) (the second) comprises the medication reservoir (16) which contains the medication (28), preferably in ionized or ionizable form, to be administered by iontophoresis.

Stabilizing reagent for membrane immunoassay

STABILIZING REAGENT FOR MEMBRANE IMMUNOASSAY ABSTRACT OF THE DISCLOSURE A method for immunoassay for a ligand is performed on a porous membrane wherein the signal detected is development of a color on the membrane. The color of the signal and background is substantially stopped and stabilized for reading of the assay -at a later time by passing a color stabilizing reagent which includes an acid through the membrane.

Fluorescent pH indicators

Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.

Substrate composition for alkaline phosphatase and method for assay using same

A composition contains levamisole and a substrate for alkaline phosphatase in a high pH buffer including 2-amino-2-methyl-1-propanol. The composition may be included in a kit of materials useful in performing an immunoassay in which intestinal alkaline phosphatase is the label. The invention includes a method for detection of a viral antigen by an immunoassay including intestinal alkaline phosphatase as the label and the composition of the invention to provide stabilized levamisole to inhibit nonintestinal alkaline phosphatase.

Fluorescent ph indicators

Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.

USER-ACTIVATED IONTOPHORETIC DEVICE

The iontophoretic device or patch (10) of the present invention includes an electrode assembly (12), having at least one electrode, an electrode reservoir (14) and at least one drug reservoir (16), which are held or contained within a pouch or other suitable structure (18). It should be appreciated that a return electrode may be combined in the assembly (12) or separately provided as is well known in the art. The device is divided or otherwise separated into two portions, one portion (20) (first) includes the electrode assembly (12) and the electrode reservoir (14) with the electrode reservoir being situated adjacent to the electrode assembly and holding an electrolyte (26). The other portion (22) (second) includes the drug reservoir (16) which holds the medication or drug (28), preferably in an ionized or ionizable form, to be delivered iontophoretically.

Immunoassay on a preblocked solid surface

A method far immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate o a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

Molecules for iontophoretic delivery

Sistema de diagnóstico e método para detectar vírus influenza, composto inibidor de máscara e kit

SISTEMA DE DIAGNÓSTICO, COMPOSTOS, KIT E MÉTODOS PARA DETECTAR VÍRUS INFLUENZA. Algumas modalidades fornecidas aqui dizem respeito a ensaios combinados. Em algumas modalidades, um ensaio para identificar influenza tipo A ou influenza tipo B é combinado com um ensaio para determinar a sensibilidade de uma neuraminidase de influenza a uma droga antiviral.

Substrat-zusammensetzung für alkali-phosphatase sowie ihre verwendung bei testverfahren.

Immunoessay auf einer vorbeschichteten festen oberfläche

Immunoassay auf einer vorbeschichteten, festen oberfläche.

Benutzeraktivierte, iontophoretische vorrichtung

Moleculas para una administracion por iontoforesis.

LA INVENCION PRESENTA PEPTIDOS Y PROTEINAS PARA UN SUMINISTRO IONTOFORETICO. DE MANERA ESPECIFICA SE PRESENTAN LOS METODOS PARA EL SUMINISTRO IONTOFORETICO DE GRUPOS DE AMINOACIDOS QUE CONSISTEN EN LA MODIFICACION DE DICHOS GRUPOS DE AMINOACIDOS EN UN PUNTO ISOELECTRICO MENOR DE 4,0 APROXIMADAMENTE O MAYOR DE 7,3 APROXIMADAMENTE CON UNA CARGA ELECTROSTATICA DE MAS O MENOS 1 Y UNIDADES CON GRUPOS DE AMINOACIDOS CON PUNTOS ISOELECTRICOS MENORES DE 4,0 APROXIMADAMENTE O MAYORES DE 7,3 APROXIMADAMENTE CON UNA CARGA ELECTROSTATICA DE MAS O MENOS 1.