Microfluidic chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Microfluidic Chemical Reaction Circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

INTEGRATED MICROFLUIDICS FOR HIGHLY PARALLEL SCREENING OF CHEMICAL REACTIONS

A microfluidic system has a microfluidic mixer and a sample storage component that is in fluid connection with the microfluidic mixer. The microfluidic mixer has a mixing section; a target molecule input section that is in fluid connection with the mixing section, the target molecule input section being suitable to provide a fluid into the mixing section that contains molecules to be targeted by chemical reactions; a first reagent input section that is in fluid connection with the mixing section, the first reagent input section being structured to selectively provide a first reagent selected from a plurality of reagents to test a chemical reaction with the target molecules; a second reagent input section that is in fluid connection with the mixing section, the second reagent input section being structured to selectively provide a second reagent selected from a plurality of reagents to test a chemical reaction with the target molecules and said first reagent; and a neutral fluid input section that is in selectable fluid connection with the sample storage component, the neutral fluid input section being structured to selectively provide a neutral fluid into the sample storage component between successive samples provided to the sample storage component to separate successive samples in a stratified arrangement. 1. A microfluidic system , comprising:a microfluidic mixer; anda sample storage component that is in fluid connection with said microfluidic mixer; a mixing section,', 'a target molecule input section that is in fluid connection with said mixing section, said target molecule input section being suitable to provide a fluid into said mixing section that contains molecules to be targeted by chemical reactions,', 'a first reagent input section that is in fluid connection with said mixing section, said first reagent input section being structured to selectively provide a first reagent selected from a plurality of reagents to test a chemical reaction with said target ...

INTEGRATED MICROFLUIDIC RADIOASSAY AND IMAGING PLATFORM FOR SMALL SAMPLE ANALYSIS

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometric P-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analog F-FAC by deoxycytidine kinase (dCK) isolated from cell lysates. 1. An apparatus for performing a microfluidic radioactivity based assay , comprising:a microfluidic chip with a microchannel system and at least one reaction element adapted to capture radioactively labeled molecules; anda solid state radiation detector coupled to the microfluidic chip;wherein radiation emitted from said microfluidic chip is detected.2. An apparatus as recited in claim 1 , wherein said reaction element comprises:a plurality of reaction columns retaining affinity beads fluidly connected to the microchannel system of the microchip.3. An apparatus as recited in claim 1 , wherein said reaction element comprises:a plurality of reaction columns retaining surface functionalized beads fluidly connected to the microchannel system of the microchip.4. An apparatus as recited in claim 3 , wherein said surface functionalized beads are functionalized with a coating of a plurality of enzyme specific antibodies claim 3 , wherein enzymes disposed in a liquid flow through the reaction column bind to the antibodies on the surface of the beads.5. An apparatus as ...

SUPRAMOLECULAR MAGNETIC NANOPARTICLES

A supramolecular magnetic nanoparticle (SMNP) can be formed by self-assembly of structural components, binding components, terminating components and at least one magnetic nanoparticle. The SMNP can provide on-demand release of a cargo and act as part of an on-demand drug release system. 1. A supramolecular magnetic nanoparticle (SMNP) comprising:a plurality of structural components each including a plurality of binding elements;at least one magnetic nanoparticle each including a plurality of binding elements;a plurality of binding components each including a plurality of binding regions, wherein each of the binding regions is adapted to bind to a binding element;a plurality of terminating components each including a terminating element , wherein the terminating element is adapted to occupy a binding region; anda cargo;wherein the plurality of terminating components are present in a sufficient quantity relative to the plurality of binding regions of the plurality of binding components to terminate further binding.2. The supramolecular magnetic nanoparticle (SMNP) of claim 1 , wherein said plurality of structural components comprises at least one of a dendrimer claim 1 , branched polyethyleneimide claim 1 , linear polyethyleneimide claim 1 , polylysine claim 1 , polylactide claim 1 , polylactide-co-glycolide claim 1 , polyanhydrides claim 1 , poly-ε-caprolactones claim 1 , polymethyl methacrylate claim 1 , poly(N-isopropyl acrylamide) or polypeptides.3. The supramolecular magnetic nanoparticle (SMNP) of claim 2 , wherein said plurality of structural components comprises a dendrimer.4. The supramolecular magnetic nanoparticle (SMNP) of claim 1 , wherein said plurality of terminating components comprises at least one of polyethylene glycol claim 1 , an adamantane derivative claim 1 , target ligands claim 1 , peptides claim 1 , antibodies or proteins.530500. The supramolecular magnetic nanoparticle (SMNP) of claim 1 , wherein the supramolecular magnetic nanoparticle ( ...

Catalytic delivery nanosubstrates (cdns) for highly efficient delivery of biomolecules

This invention relates, e.g., to a molecular delivery system comprising A. a substrate having a nanostructured surface region which comprises a plurality of nanostructures and, covalently attached to the substrate, multiple copies of a first member of a binding pair; and B. at least one vector nanoparticle which comprises, encapsulated therein, a molecule of interest, and on its surface, multiple copies of second member of the binding pair. Methods of using the molecular delivery system to deliver a molecule of interest to a cell are also described.

Phenotypic profiling of hepatocellular carcinoma circulating tumor cells for treatment selection

Methods and kits for detecting hepatocellular carcinoma recurrence or metastasis, and of measuring markers of hepatocellular carcinoma, including markers of hepatocellular carcinoma recurrence or metastasis, in a blood sample obtained from a subject by (a) isolating circulating tumor cells (CTCs) by contacting a blood sample obtained from the subject with a set of capture antibodies, wherein the capture antibodies specifically bind asialoglycoprotein receptor (ASGPR), Glypican-3, and epithelial cell adhesion molecule (EpCAM); (b) contacting the isolated CTCs with an antibody that specifically binds vimentin; and (c) measuring the number of vimentin-positive CTC.

DEVICE FOR CAPTURING CIRCULATING CELLS

The present invention provides devices and methods for capturing rare cells. The devices and methods described herein can be used to facilitate the diagnosis and monitoring of metastatic cancers. 147-. (canceled)48. A method of selectively isolating biological cells from a cell sample comprising:contacting the cell sample to a device for capturing biological cells under conditions that permit binding agents of the device to selectively bind a subpopulation of biological cells, resulting in bound biological cells; andremoving unbound biological cells from the device, a substrate comprising a nanostructured surface region; and', 'a plurality of binding agents attached to the nanostructured surface region of the substrate,', 'wherein the nanostructured surface region comprises a plurality of nanostructures each having a longitudinal dimension and a lateral dimension, the longitudinal dimension being at least ten times greater than the lateral dimension, and', 'wherein biological cells are selectively captured by the binding agents and the plurality of nanostructures acting in cooperation., 'wherein the device comprises49. A method according to claim 48 , further comprising washing the captured biological cells with an aqueous medium.50. A method according to claim 48 , wherein the cell sample comprises one or more of body fluid claim 48 , plasma claim 48 , saliva claim 48 , spinal fluid claim 48 , and urine.51. A method according to claim 48 , wherein the subpopulation of biological cells comprises circulating tumor cells.52. A method of selectively isolating at least one target biological cell from a cell sample comprising:providing a cell sample comprising at least one target biological cell; andcontacting the cell sample to a plurality of nanostructures to result in the target biological cell(s) being captured,wherein a plurality of binding agents specific for and which bind to the target biological cell(s) are attached to the nanostructures;under conditions ...

SYSTEMS, METHODS AND COMPONENTS FOR ISOLATING CELLS FROM A FLUID SAMPLE

A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip. 1. A system for isolating preselected cell types from a fluid sample comprising a plurality of cell types , said system comprising:a cell-capture fluidic chip; andchip holder configured to receive said cell-capture fluidic chip and to maintain said cell capture fluidic chip with a substantially fluid-tight seal while in operation,wherein said chip holder is further configured to release said cell-capture fluidic chip to be removed from said chip holder for further processing, a substrate,', 'a laser micro-dissection membrane disposed on said substrate, and', 'a channel-defining layer disposed on said laser micro-dissection membrane, wherein said laser micro-dissection membrane has a surface comprising at least one of a structural or a chemical modification to capture said preselected cell types preferentially over other cell types of said plurality of cell types,, 'wherein said cell-capture fluidic chip compriseswherein said channel-defining layer is removable from said laser micro-dissection membrane for further processing of said cell- ...

Selective capture and stimulated release of circulating tumor cells on nanostructured devices

A device for capturing preselected cell types from a fluid sample that includes a plurality of cell types includes a substrate, a plurality of nanowires at least one of attached to or integral with a surface of the substrate such that each nanowire of the plurality of nanowires has an unattached end, and a layer of temperature-responsive material formed on at least the unattached end of each of the plurality of nanowires. The layer of temperature-responsive material has a compact configuration at a first temperature and an expanded configuration at a second temperature so as to facilitate release of cells captured at the first temperature to be released at the second temperature.

SYSTEMS, METHODS AND COMPONENTS FOR ISOLATING CELLS FROM A FLUID SAMPLE

A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip. 1. A system for isolating preselected cell types from a fluid sample comprising a plurality of cell types , said system comprising:a cell-capture fluidic chip; andchip holder configured to receive said cell-capture fluidic chip and to maintain said cell capture fluidic chip with a substantially fluid-tight seal while in operation,wherein said chip holder is further configured to release said cell-capture fluidic chip to be removed from said chip holder for further processing, a substrate,', 'a laser micro-dissection membrane disposed on said substrate, and', 'a channel-defining layer disposed on said laser micro-dissection membrane,, 'wherein said cell-capture fluidic chip compriseswherein said laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of said plurality of cell types,wherein said channel-defining layer is removable from said laser micro-dissection membrane for further processing of said cell-capture fluidic chip, andwherein said cell-capture fluidic chip ...

METHOD OF ASSESSING DISEASE CONDITION OF CANCER

A method, system and computer-readable medium for assessing a disease condition of a cancer of a subject, including: receiving a blood sample from the subject; isolating a plurality of circulating tumor cells (CTCs) from the blood sample; measuring at least one of cell or cell nucleus sizes of each of the plurality of CTCs; determining a measured CTC size distribution of the plurality of CTCs based on the measuring; comparing the measured CTC size distribution to a reference CTC size distribution using a computer; and assigning the disease condition of the cancer of the subject based on the comparing. 1. A method of assessing a disease condition of a cancer of a subject , comprising:receiving a blood sample from said subject;isolating a plurality of circulating tumor cells (CTCs) from said blood sample;measuring at least one of cell or cell nucleus sizes of each of said plurality of CTCs;determining a measured CTC size distribution of said plurality of CTCs based on said measuring;comparing said measured CTC size distribution to a reference CTC size distribution using a computer; andassigning said disease condition of said cancer of said subject based on said comparing.2. The method of claim 1 , wherein said assigning is an automated assigning performed by said computer.3. The method of claim 1 , wherein said isolating said plurality of CTCs is performed using a device that has a nanostructured surface that enhances capture of CTCs preferentially over other types of cells.4. The method of claim 1 , wherein said reference distribution is an empirically obtained size distribution that is resolved into a plurality of size-cluster distributions.5. The method of claim 4 , wherein said plurality of size-cluster distributions is three different size-cluster distributions corresponding to large-nuclear CTCs claim 4 , small-nuclear CTCs and very-small-nuclear CTCs claim 4 , respectively.6. The method of claim 1 , wherein said assigning said disease condition of said cancer ...

Guided magnetic nanostructures for targeted and high-throughput intracellular delivery

A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.

Device for quantification of radioisotope concentrations in a micro-fluidic platform

A micro-fluidic device has a micro-fluidic circuit layer and a charged-particle detection layer disposed proximate the micro-fluidic circuit layer. The micro-fluidic device is constructed to provide a two-dimensional image of charged-particle emissions from a sample within the micro-fluidic circuit layer while in operation. A method of quantification of radioactivity in a biological sample includes directing a fluid containing the biological material into a microfluidic device, detecting charged particles emitted from the biological material with a two-dimensional imaging sensor, and forming a two-dimensional image over time corresponding to radioactivity of the biological sample.

Integrated microfluidic radioassay and imaging platform for small sample analysis

An immunocapture-based in vitro kinase assay on an integrated polydimethylsiloxane (PDMS) microfluidics platform that can reproducibly measure kinase activity from as few as 3,000 cells is described. For this platform, the standard radiometric 32 P-ATP labeled phosphate transfer assay was adopted. Implementation on a microfluidic device required the development of methods for repeated trapping and mixing of solid-phase affinity micro beads. A solid state beta-particle camera imbedded directly below the microfluidic device was used to provide real-time quantitative detection of the signal from this and other microfluidic radio bioassays. The integrated device can measure ABL protein kinase activity from BCR-ABL positive leukemia patient samples, and can measure the small molecule phosphorylation such as phosphorylation of the deoxycytidine analog 18 F-FAC by deoxycytidine kinase (dCK) isolated from cell lysates.

Microfluidic imaging cytometry

A microfluidic system has a pipette system comprising a plurality of pipettes, a microfluidic chip arranged proximate the pipette system, an imaging optical detection system arranged proximate the microfluidic chip, and an image processing system in communication with the imaging optical detection system. The microfluidic chip has a plurality of cell culture chambers defined by a body of the microfluidic chip, each cell culture chamber being in fluid connection with an input channel and an output channel defined by the microfluidic chip. The pipette system is constructed and arranged to at least one of inject fluid through the plurality of pipettes into the plurality of input channels or extract fluid through the plurality of pipettes from the plurality of output channels while the microfluidic system is in operation.

Microfluidic devices with chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Microfluidic devices with chemical reaction circuits

New microfluidic devices, useful for carrying out chemical reactions, are provided. The devices are adapted for on-chip solvent exchange, chemical processes requiring multiple chemical reactions, and rapid concentration of reagents.

Integrated Microfluidics for Parallel Screening of Chemical Reactions

A microfluidic device allows for different reactions to be conducted in parallel with the use of nanoliter quantities of reagents.

Integrated microfluidics for highly parallel screening of chemical reactions

A microfluidic system has a microfluidic mixer and a sample storage component that is in fluid connection with the microfluidic mixer. The microfluidic mixer has a mixing section; a target molecule input section that is in fluid connection with the mixing section, the target molecule input section being suitable to provide a fluid into the mixing section that contains molecules to be targeted by chemical reactions; a first reagent input section that is in fluid connection with the mixing section, the first reagent input section being structured to selectively provide a first reagent selected from a plurality of reagents to test a chemical reaction with the target molecules; a second reagent input section that is in fluid connection with the mixing section, the second reagent input section being structured to selectively provide a second reagent selected from a plurality of reagents to test a chemical reaction with the target molecules and said first reagent; and a neutral fluid input section that is in selectable fluid connection with the sample storage component, the neutral fluid input section being structured to selectively provide a neutral fluid into the sample storage component between successive samples provided to the sample storage component to separate successive samples in a stratified arrangement.

Device for capturing circulating cells

The present invention provides devices and methods for capturing rare cells. The devices and methods described herein can be used to facilitate the diagnosis and monitoring of metastatic cancers.

Supramolecular magnetic nanoparticles

Method and device for microreactor pressure control

A microfluidic device has a device body, wherein the device body defines a microfluidic reactor and a pressure control system therein, the pressure control system being arranged proximate the microfluidic reactor. The microfluidic device also has a fluid-permeable membrane arranged between the microfluidic reactor and the pressure control system, the fluid-permeable membrane having a surface area and thickness such that a fluid can pass from a high pressure side to a low pressure side of said fluid-permeable membrane during operation of the microfluidic device to at least one of change a pressure in the microfluidic reactor in a preselected manner, to maintain a substantially constant pressure in the microfluidic reactor, to change a composition of fluids in the microfluidic reactor, or maintain a composition of fluids in the microfluidic reactor. A method on performing chemical reactions includes introducing a plurality of chemical substances into a microfluidic reactor, wherein the microfluidic reactor has a fluid-permeable membrane between at least a portion of the microfluidic reactor and a microfluidic pressure control system; and at least one of pressurizing with a fluid or evacuating a region of the pressure control system proximate the fluid-permeable membrane to cause fluid to pass through the fluid-permeable membrane in a predetermined manner in order to cause a predetermined effect on a chemical reaction in the microfluidic reactor.

Guided magnetic nanostructures for targeted and high-throughput intracellular delivery

A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.

Covalent chemistry enables extracellular vesicle purification on nanosubstrates - toward early detection of hepatocellular carcinoma

Methods for selectively capturing an extracellular vesicle (EV) from a sample, including the steps of: functionalizing a capture agent for the EV with a first molecule from a first bioorthogonal functional group such that the capture agent remains attachable to the EV and the first molecule is also able to bond to a second molecule from a second bioorthogonal functional group, the second molecule being complementary to the first molecule; mixing the functionalized capture agent with the sample such that the functionalized capture agent binds to the EV and such that an activated sample is formed; functionalizing a capture surface with the second molecule; and depositing at least a portion of the activated sample on at least a portion of the functionalized capture surface to thereby selectively capture the EV by binding of the second molecule with the first molecule.

A supramolecular approach for preparation of size controllable nanoparticles

A supramolecular approach has been developed for preparation of size-controllable nanoparticles, from three different molecular building blocks.

Cross-linked supramolecular nanoparticles for controlled release of antifungal drugs and steroids - a new therapeutic approach for onychomycosis and keloid

Compositions for delivering a drug to a subject having: a plurality of self-assembled supramolecular nanoparticles (SMNPs), each of the plurality of self-assembled supramolecular nanoparticles (SMNPs) having: a plurality of binding components, each having a plurality of binding regions; a plurality of cores that are suitable to at least provide some mechanical structure to the plurality of self-assembled supramolecular nanoparticles (SMNPs), the plurality of cores comprising at least one core binding element adapted to bind to the binding regions to form a first inclusion complex; a plurality of terminating components, each having a single terminating binding element that binds to remaining binding regions of one of said plurality of binding components by forming a second inclusion complex; the drug; and a reporter agent, and methods of use thereof.

Extracellular vesicle protein assay for noninvasive diagnostics

The embodiments of the present invention relate to devices, methods and kits for assaying a disease in a subject, by selectively capturing extracellular vesicles (EVs) by click chemistry with functionalized antibodies, selectively labeling the EVs with a plurality of DNA barcodes conjugated antibodies, optionally releasing the plurality of DNA barcodes, and assaying the plurality of DNA barcodes to determine whether the disease is present or predict the stage of the disease in the subject.

Dual supramolecular nanoparticle vectors enable crispr/cas9-mediated knockin of retinoschisin 1 gene-a potential non-viral therapeutic solutions for x-linked juvenile retinoschisis

Compositions, systems and methods for delivering CRISPR/Cas9-based genome editing system and a donor protein to a cell.

Covalent chemistry enables extracellular vesicle purification on nanosubstrates ? toward early detection of hepatocellular carcinoma

Methods for selectively capturing an extracellular vesicle (EV) from a sample. including the steps of: functionalizing a capture agent for the EV with a first molecule from a first bioorthogonal functional group such that the capture agent remains attachable to the EV and the first molecule is also able to bond to a second molecule from a second bioorthogonal functional group. the second molecule being complementary to the first molecule: mixing the functionalized capture agent with the sample such that the functionalized capture agent binds to the EV and such that an activated sample is formed: functionalizing a capture surface with the second molecule: and depositing at least a portion of the activated sample on at least a portion of the functionalized capture surface to thereby selectively capture the EV by binding of the second molecule with the first molecule.

Cross-linked supramolecular nanoparticles for controlled release of antifungal drugs and steroids - a new therapeutic approach for onychomycosis and keloid

Compositions for delivering a drug to a subject having: a plurality of self-assembled supramolecular nanoparticles (SMNPs), each of the plurality of self-assembled supramolecular nanoparticles (SMNPs) having: a plurality of binding components, each having a plurality of binding regions; a plurality of cores that are suitable to at least provide some mechanical structure to the plurality of self-assembled supramolecular nanoparticles (SMNPs), the plurality of cores comprising at least one core binding element adapted to bind to the binding regions to form a first inclusion complex; a plurality of terminating components, each having a single terminating binding element that binds to remaining binding regions of one of said plurality of binding components by forming a second inclusion complex; the drug; and a reporter agent, and methods of use thereof.

Selective capture and stimulated release of circulating cells on nanostructured devices

A device for capturing preselected cell types from a fluid sample that includes a plurality of cell types includes a substrate, a plurality of nanowires at least one of attached to or integral with a surface of the substrate such that each nanowire of the plurality of nanowires has an unattached end, and a layer of temperature-responsive material formed on at least the unattached end of each of the plurality of nanowires. The layer of temperature-responsive material has a compact configuration at a first temperature and an expanded configuration at a second temperature so as to facilitate release of cells captured at the first temperature to be released at the second temperature.

Dual supramolecular nanoparticle vectors enable crispr/cas9-mediated knockin of retinoschisin 1 gene-a potential non-viral therapeutic solutions for x-linked juvenile retinoschisis

Dual supramolecular nanoparticle vectors enable crispr/cas9-mediated knockin of retinoschisin 1 gene-a potential non-viral therapeutic solutions for x-linked juvenile retinoschisis

Compositions, systems and methods for delivering CRISPR/Cas9-based genome editing system and a donor protein to a cell.

Catalytic delivery nanoubstrates (cdns) for highly efficient delivery of biomolecules

This invention relates, e.g., to a molecular delivery system comprising A. a substrate having a nanostructured surface region which comprises a plurality of nanostructures and, covalently attached to the substrate, multiple copies of a first member of a binding pair; and B. at least one vector nanoparticle which comprises, encapsulated therein, a molecule of interest, and on its surface, multiple copies of second member of the binding pair. Methods of using the molecular delivery system to deliver a molecule of interest to a cell are also described.

Microfluidic samplers and methods for making and using them

This invention provides microfluidic samplers for withdrawing one or more precise micro- or nano-liter volumes of a sample. The invention provides microfabricated automatic systems comprising integrated poly(dimethyl-siloxane) (PDMS) micro fluidics. The sample can be biological samples, including samples from animals or plants. The samples can be fluid or gas. The samples can comprise a biological fluid, such as blood, tears, cerebral spinal fluid (CSF) and the like, from a test subject such as a human or a mouse. The invention also provides methods for making and using the microfluidic samplers of the invention.

Circulating trophoblasts as a new biomarker for risk assessment of morbidly adherent placentation

Methods and kits for isolating at least one of a plurality of single circulating trophoblasts (cTBs) and a plurality of clustered circulating trophoblasts (cTBs) from a blood sample and determining whether a subject has a placenta accrete spectrum (PAS) disorders.

Circulating trophoblasts as a new biomarker for risk assessment of morbidly adherent placentation

Methods and kits for isolating at least one of a plurality of single circulating trophoblasts (cTBs) and a plurality of clustered circulating trophoblasts (cTBs) from a blood sample and determining whether a subject has a placenta accrete spectrum (PAS) disorders.

Circulating trophoblasts as a new biomarker for risk assessment of morbidly adherent placentation

Methods and kits for isolating at least one of a plurality of single circulating trophoblasts (cTBs) and a plurality of clustered circulating trophoblasts (cTBs) from a blood sample and determining whether a subject has a placenta accrete spectrum (PAS) disorders.

Supramolecular approach for preparation of size controllable nanoparticles

A supramolecular approach has been developed for preparation of size-controllable nanoparticles, from three different molecular building blocks.

Device for capturing circulating cells

The present invention provides devices and methods for capturing rare cells. The devices and methods described herein can be used to facilitate the diagnosis and monitoring of metastatic cancers.