25-04-2013 дата публикации
Номер: US20130102022A1
Provided herein are plants having altered expression of a GAUT polypeptide. Such plants have phenotypes that may include decreased recalcitrance, increased growth, decreased lignin content, or a combination thereof. Also provided herein are methods of making and using such plants. 1. A method for using a transgenic plant , the method comprising processing a transgenic plant to result in pulp , wherein the transgenic plant comprises decreased expression of a coding region encoding a GAUT polypeptide compared to a control plant.2. The method of wherein the processing comprises a physical pretreatment claim 2 , a chemical pretreatment claim 2 , or a combination thereof.3. The method of further comprising hydrolyzing the processed pulp.4. The method of further comprising contacting the processed pulp with an ethanologenic microbe.5. The method of wherein the ethanologenic microbe is a eukaryote.6. The method of further comprising obtaining a metabolic product.7. The method of wherein the metabolic product comprises ethanol.8. The pulp of .9. A method comprising hydrolyzing a pulp claim 1 , wherein the pulp comprises cells of a transgenic plant claim 1 , wherein the cells comprise a mutation in a coding region encoding a GAUT polypeptide.10. The method of wherein the hydrolyzing comprises contacting the pulp with a composition comprising a cellulase under conditions suitable for hydrolysis.11. The method of further comprising contacting the hydrolyzed pulp with an ethanologenic microbe.12. The method of wherein the ethanologenic microbe is a eukaryote.13. The method of further comprising obtaining a metabolic product.14. The method of wherein the metabolic product comprises ethanol.15. A method for producing a metabolic product comprising:contacting under conditions suitable for the production of a metabolic product a microbe with a composition comprising a pulp obtained from a transgenic plant, wherein the transgenic plant comprises decreased expression of a coding ...
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