18-01-2018 дата публикации
Номер: US20180016646A1
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The present invention provides methods and compositions for the identification and selection of loci modulating phenotypic expression of a chloride tolerant trait in plant breeding. In addition, methods are provided for screening germplasm entries for the performance and expression of this trait. 16.-. (canceled)7. A method for obtaining a soybean plant comprising in its genome at least one chloride tolerance locus , compromising the steps of:a. genotyping a plurality of soybean plants with respect to at least one genetic locus on chromosome 3 flanked by loci NGMAX006180041 (SEQ ID NO: 4) and NGMAX006184463 (SEQ ID NO:34); andb. selecting a soybean plant comprising in its genome at least one genetic locus comprising a genotype associated with chloride tolerance.8. The method of claim 7 , wherein said genotype associated with chloride tolerance comprises at least one polymorphic allele of at least one marker selected from the group consisting of NS0096117 (SEQ ID NO:8) claim 7 , NS0205902 (SEQ ID NO: 13) claim 7 , and NS0203171 (SEQ ID NO: 31).9. The method of claim 8 , wherein said marker comprises a GG allele of NS0203171 (SEQ ID NO: 31).10. The method of claim 8 , wherein said marker comprises a TT allele of NS0205902 (SEQ ID NO: 13)11. The method of claim 7 , wherein said plurality of soybean plants comprises a population that is obtained by: i) crossing a parent plant comprising at least one chloride tolerance locus with a parent plant comprising at least one chloride sensitivity locus; or claim 7 , ii) obtaining seed or progeny from a parental plant segregating for at least one chloride tolerance locus.12. The method of claim 7 , wherein said population contains plants that contain a transgene that confers resistance to dicamba and/or a transgene that confers resistance to glyphosate.13. The method of claim 7 , further comprising the step of assaying for the presence of at least one additional marker claim 7 , wherein said additional marker is either linked or ...
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