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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 90. Отображено 90.
28-03-2013 дата публикации

Signal and detection system for keying applications

Номер: AU2011301170A1
Принадлежит:

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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09-07-2009 дата публикации

PRODUCT, DISPENSER AND METHOD OF DISPENSING PRODUCT

Номер: CA0002709996A1

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount.

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10-03-2020 дата публикации

DIFFRACTION BASED BIOSENSOR CONTAINING TWO DIFFRACTIVE GRATINGS

Номер: CA0002903282C
Принадлежит: AXELA INC, AXELA INC.

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

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01-05-2018 дата публикации

METHOD FOR PURIFICATION OF NUCLEIC ACIDS, PARTICULARLY FROM FIXED TISSUE

Номер: CA0002746489C

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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19-06-1997 дата публикации

ELECTROPHORESIS GELS AND GEL HOLDERS HAVING ADHESIVE AFFIXED FIBER SPACERS AND METHOD OF MAKING SAME

Номер: CA0002239982A1
Принадлежит: Individual

Gel holders for electrophoresis gels are made using fibers, particularly glass fibers, which are affixed to the substrates forming the gel holder using an adhesive. These gel holders can be made by placing a plurality of adhesivecoated fibers between a first planar substrate and a second planar substrate; and applying pressure to the outside of the substrates to adhere the fibers to the first and second substrates. This forms a gel chamber between the first and second substrates which has a thickness defined by diameter of the fibers. Alternatively, uncoated fibers may be laid down in pairs, with a line of adhesive disposed between each fiber of the pair. When the adhesive is cured, it binds the fibers in position as spacers. At the same time, the fibers isolate the adhesive from the gel compartment. In this way, interference of components of the adhesive with the polymerization of the gel in the gel chamber can be avoided. Gel holders formed using either of these methods may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely and used as needed.

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16-11-2011 дата публикации

Method for purification of nucleic acids, particularly from fixed tissue

Номер: CN0102245770A
Принадлежит:

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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14-05-2009 дата публикации

A METHOD AND APPARATUS FOR INCREASING THE SENSITIVITY OF A BIOSENSOR USED IN A PLANAR WAVEGUIDE

Номер: CA0002704779A1
Принадлежит: BAYER TECHNOLOGY SERVICES GMBH

Systems, methods and apparatus are provided for mixing an analyte in a planar waveguide cartridge. The invention includes adding magnetic particles to an analyte containing one or more types of target molecules; inserting the analyte and magnetic particles into the cartridge; and moving a magnetic field proximate to and around the cartridge containing the analyte and magnetic particles, wherein the movement of the magnet field causes movement in the analyte. Numerous other aspects are provided.

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17-06-2010 дата публикации

METHOD FOR PURIFICATION OF NUCLEIC ACIDS, PARTICULARLY FROM FIXED TISSUE

Номер: CA0002746489A1
Принадлежит: Siemens Healthcare Diagnostics Inc

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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04-09-2014 дата публикации

Signal and detection system for keying applications

Номер: AU2011301170B2
Принадлежит: SMART WAVE TECHNOLOGIES Inc

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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19-06-1997 дата публикации

ELECTROPHORESIS GELS AND GEL HOLDERS HAVING FIBER SPACERS AND METHOD OF MAKING SAME

Номер: CA0002239988A1
Принадлежит:

Gel holders for electrophoresis gels are made using clad fibers, particularly glass fibers as spacers between substrates. A plurality of fibers with a highmelting interior core and a low-melting external cladding are placed between a first planar substrate and a second planar substrate. The fibers are heated to a temperatrure sufficient to at least soften the exterior cladding of the fibers without softening the interior core of the fibers, and then cooled while they are in contact with the first and second substrates to resolidify the exterior cladding. This adheres the fibers to the first and second substrates, and forms a gel chamber between said first and second substrates. The gel chamber has a thickness defined by interior core of the fibers. The fibers may be heated before or after the second substrate is placed over the top of the fibers. The gel holders thus formed may be filled immediately with a gel forming solution such as a polyacrylamide, or they may be stored indefinitely ...

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18-09-2014 дата публикации

DIFFRACTION BASED BIOSENSOR CONTAINING TWO DIFFRACTIVE GRATINGS

Номер: CA0002903282A1
Принадлежит:

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

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15-03-2012 дата публикации

SIGNAL AND DETECTION SYSTEM FOR KEYING APPLICATIONS

Номер: CA0002810701A1
Принадлежит:

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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20-06-2017 дата публикации

SIGNAL AND DETECTION SYSTEM FOR KEYING APPLICATIONS

Номер: CA0002810701C

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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24-09-1998 дата публикации

HIGH DYNAMIC RANGE APPARATUS FOR SEPARATION AND DETECTION OFPOLYNUCLEOTIDE FRAGMENTS

Номер: CA0002284888A1
Принадлежит:

A high dynamic range apparatus for separation and detection of polynucleotide fragments has a housing adapted to receive an electrophoresis gel holder containing an electrophoresis gel loaded with fluorophore-labeled samples; one or more laser diodes for providing radiation of a frequency suitable for excitation of the fluorophore which irradiates an array of excitation/detection sites on the electrophoresis gel; an array of detectors aligned with the excitation/detection sites for collecting fluorescent emissions; and one or more components for increasing the dynamic range of the instrument by at least an order of magnitude. These components, which can be used individually or in combination include detectors that are connected to a signal processing system that modulates the period of signal integration employed so that large signals are totaled at short time intervals and smaller signals are totaled at longer time intervals; the use of a beam splitter to produce a high intensity beam ...

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03-07-2014 дата публикации

METHOD FOR PURIFICATION OF NUCLEIC ACIDS, PARTICULARLY FROM FIXED TISSUE

Номер: US20140186236A1
Принадлежит: Siemens Healthcare Diagnostics Inc.

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution. 2. A sample vessel holder for accommodating the at least one sample vessel , comprising at least one annular magnet , in the annular interior space of which the sample vessel can be accommodated.3. The sample vessel holder as claimed in claim 2 , wherein the ring inner diameter of the annular magnet is claim 2 , at its narrowest point claim 2 , 4 to 20 mm claim 2 , preferably 6-12 claim 2 , in particular 8 mm. This application is a divisional of U.S. application Ser. No. 13/133,182 which was filed on Jun. 7, 2011 which is the US National Stage of International Application No. PCT/EP2009/065534 filed Nov. 20, 2009. The International Application claims the benefit of German Patent Office Application No. 102008061714.8 filed Dec. 12, 2008. All of the applications are incorporated by reference herein in their entirety.The invention relates to a method for purifying nucleic acids, to a system for carrying out the method according to the invention, and also to a sample vessel holder for the purification of a biological sample.Molecular diagnostics has recently become increasingly important. It has found a way into the clinical diagnosis of diseases (inter alia, detection of infectious agents, detection ...

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23-05-2013 дата публикации

Signal and detection system for keying applications

Номер: AU2011301170A8

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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13-10-2016 дата публикации

DISPOSABLE BIOASSAY CARTRIDGE AND METHOD OF PERFORMING MULTIPLE ASSAY STEPS AND FLUID TRANSFER WITHIN THE CARTRIDGE

Номер: CA0002981990A1
Принадлежит:

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins.

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21-10-2010 дата публикации

DIAGNOSTIC DEVICES AND RELATED METHODS

Номер: CA0002758526A1
Принадлежит:

Devices, systems, and methods for detecting the presence of one or more analytes in a sample are described. In some variations, a test strip may be used to detect and/or analyze one or more analytes in a sample. In certain variations, a test strip configured to receive a sample for detection of an analyte therein may comprise a substrate and a coating on a portion of the substrate, the coating comprising a combination of a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte.

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21-10-2010 дата публикации

DIAGNOSTIC DEVICES AND RELATED METHODS

Номер: US20100267049A1
Принадлежит:

Devices, systems, and methods for detecting the presence of one or more analytes in a sample are described. In some variations, a test strip may be used to detect and/or analyze one or more analytes in a sample. In certain variations, a test strip configured to receive a sample for detection of an analyte therein may comprise a substrate and a coating on a portion of the substrate, the coating comprising a combination of a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte.

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05-06-2018 дата публикации

Diffraction based biosensor containing two diffractive gratings

Номер: US0009989525B2
Принадлежит: AXELA INC., AXELA INC

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

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16-08-2016 дата публикации

Method for purification of nucleic acids, particularly from fixed tissue

Номер: US0009416399B2

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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24-04-2012 дата публикации

Product, dispenser and method of dispensing product

Номер: US0008165716B1

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount. To determine if the sheet product is authorized, a pigment is excited to emit a light with an intensity signature. A portion of the intensity signature is measured and compared to a desired value. If the portion is substantially equal to the desired value, the sheet product is authenticated.

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18-09-2014 дата публикации

DIFFRACTION BASED BIOSENSOR CONTAINING TWO DIFFRACTIVE GRATINGS

Номер: US2014271363A1
Принадлежит:

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

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10-03-2016 дата публикации

Diagnostic devices and related methods

Номер: AU2010236424B2
Принадлежит:

Devices, systems, and methods for detecting the presence of one or more analytes in a sample are described. In some variations, a test strip may be used to detect and/or analyze one or more analytes in a sample. In certain variations, a test strip configured to receive a sample for detection of an analyte therein may comprise a substrate and a coating on a portion of the substrate, the coating comprising a combination of a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte.

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25-10-2016 дата публикации

Signal and detection system for keying applications

Номер: US0009476826B2

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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29-12-2016 дата публикации

Signal and Detection System For Keying Applications

Номер: US20160377550A1
Принадлежит:

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product. 122-. (canceled)23. A second product comprising an optical coating on a substrate ,wherein the second product is a consumable product shaped to engage with a first product, the first product being a dispenser having a receiver and a transmitter and configured to direct light to the optical coating,and wherein the second product is configured such that the optical coating is positionable between the optical transmitter and the receiver of the first product while authorizing pairing of a first product with a second product such that: interpret reflected light received at the receiver against an authorized signal by analysis of the spectral response including analysis of the degree of absorption of the reflected light at the optical coating, to thereby determine whether the reflected light received at the receiver matches the authorized signal;', 'in response to determining that the reflected light received at the receiver matches the authorized signal, authorize pairing of the second product with the first product to allow the dispensing of the second product by the first product; and', 'in response to determining that reflected light received at the receiver does not match the authorized signal, prevent pairing of the second product with the first product to prevent the dispensing of the second product by the first product., 'the optical coating partially reflects light received from the transmitter to the receiver to enable the first product to24. The keying system of claim 23 , wherein the transmitter is an LED light source.25. The keying system of claim 23 , wherein the second product comprises two or more optical coatings and the first ...

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17-06-2010 дата публикации

Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe

Номер: DE102008061714A1
Принадлежит: Siemens Healthcare Diagnostics Inc

Die Erfindung betrifft ein Verfahren zur Aufreinigung von Nukleinsäuren, ein Kit, um das erfindungsgemäße Verfahren durchzuführen, sowie eine neue Verwendung von Magnetpartikeln zur Aufreinigung einer biologischen Probe. Das erfindungsgemäße Verfahren umfasst die folgenden Schritte: a) Aufnehmen der Probe in einem ersten Probengefäß in einer wässrigen Lösung und Lysieren der Probe unter nicht-chaotropen Bedingungen; Suspendieren von ersten magnetischen Partikeln in der Lösung und Einbringen des ersten Probengefäßes in eine Probegefäßhalterung, wobei das Probengefäß in den Ringinnenraum eines mit der Probegefäßhalterung assoziierten Ringmagneten eingebracht wird; Abtrennen der Lösung von den magnetischen Partikeln; und Isolieren der Nukleinsäuren aus der Lösung.

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06-10-2010 дата публикации

A method and apparatus for increasing the sensitivity of a biosensor used in a planar waveguide

Номер: CN0101855536A
Принадлежит:

Systems, methods and apparatus are provided for mixing an analyte in a planar waveguide cartridge. The invention includes adding magnetic particles to an analyte containing one or more types of target molecules; inserting the analyte and magnetic particles into the cartridge; and moving a magnetic field proximate to and around the cartridge containing the analyte and magnetic particles, wherein the movement of the magnet field causes movement in the analyte. Numerous other aspects are provided.

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27-08-2002 дата публикации

Apparatus for sequencing of nucleic acids using multiple dyes

Номер: US0006440664B1

An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.

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13-08-2002 дата публикации

Method, apparatus and kits for sequencing of nucleic acids using multiple dyes

Номер: US0006432634B1

An instrument for sequencing oligonucleotides is loaded with the products of four sequencing reaction mixtures. These products are a combination of A, C, G and T reaction products for several sequencing reactions. The products of the different sequencing reactions are labeled with fluorescent tags which are distinguishable one from the other on the basis of their excitation or emission spectra. After separation of the oligonucleotides by electrophoresis, the order of the detected peaks is used to call the base sequence.

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09-07-2009 дата публикации

Product, Dispenser and Method of Dispensing Product

Номер: US2009177315A1
Принадлежит:

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount.

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04-11-2010 дата публикации

Method and Apparatus for Increasing the Sensitivity of a Biosensor Used in a Planar Waveguide

Номер: US20100279429A1
Принадлежит: BAYER TECHNOLOGY SERVICES GMBH

Systems, methods and apparatus are provided for mixing an analyte in a planar waveguide cartridge. The invention includes adding magnetic particles to an analyte containing one or more types of target molecules; inserting the analyte and magnetic particles into the cartridge; and moving a magnetic filed proximate to and around the cartridge containing the analyte and magnetic particles, wherein the movement of the magnet field causes movement in the analyte.

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03-12-2009 дата публикации

METHOD FOR SIGNAL INTENSITY CORRECTION IN WAVEGUIDE SENSORS

Номер: US2009294677A1
Принадлежит:

Methods are provided for enhancing the detection of analytes with waveguides by accounting for cumulative light absorptions attributable to the presence of one or more analytes in a sample as well as the waveguide material.

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06-10-2011 дата публикации

Method For Purification Of Nucleic Acids, Particularly From Fixed Tissue

Номер: US20110245483A1
Принадлежит: SIEMENS HEALTHCARE DIAGNOSTICS INC.

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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22-04-2014 дата публикации

Method for purification of nucleic acids, particularly from fixed tissue

Номер: US0008703931B2

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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16-03-2021 дата публикации

Disposable bioassay cartridge and method of performing multiple assay steps and fluid transfer within the cartridge

Номер: US0010946375B2

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins.

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21-02-2019 дата публикации

DISPOSABLE BIOASSAY CARTRIDGE AND METHOD OF PERFORMING MULTIPLE ASSAY STEPS AND FLUID TRANSFER WITHIN THE CARTRIDGE

Номер: US20190054460A1
Принадлежит:

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins. 138-. (canceled)39. A porous substrate for detection of surface bound substances , comprising:a generally planar microporous substrate material having opposed surfaces and pores extending through a thickness of said substrate in which the pores are wider near one surface of the substrate compared to a width of the pores on the opposed surface thereby improving the collection efficiency of light emitted from optical probes bound to the interior surfaces of the widened pores.40. The porous substrate according to in which the pores are progressively wider near one surface of the substrate.41. The porous substrate according to in which the pores have a rectangular cross section.42. The porous substrate according to in which the pores have a square cross section.43. The porous substrate according to in which the pores have a circular cross section.44. The porous substrate according to in which the tapering is conical.45. The porous substrate according to in which the tapering is spherical.46. The porous substrate according to in which the tapering is parabolic.47. The porous substrate according to in which the pore dimension on the side opposite the side of the widened pores are substantially smaller thereby providing structural stability.48. The porous substrate according to which the pores are of uniform dimensions and morphology.49. The porous substrate according to further comprising reinforcement ribs to provide structural stability.50. The porous substrate according to in which the reinforcement ribs are an integral part of the substrate.51. The ...

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10-11-2010 дата публикации

Product, dispenser and method of dispensing product

Номер: CN0101883514A
Принадлежит:

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount.

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06-07-2004 дата публикации

Method for alignment of DNA sequences with enhanced accuracy and read length

Номер: US0006760668B1
Принадлежит: Bayer HealthCare LLC, BAYER HEALTHCARE LLC

In order to align DNA sequence data traces, an experimental data trace representing the positions of a first species of base within a target polynucleotide and a reference data trace representing the positions of a second species of base (which may be the same as or different from the first species) within a reference polynucleotide are obtained by separating appropriate sequencing fragments generated from the target and reference polynucleotides on an electrophoresis gel. For each reference data trace, a plurality of peaks corresponding to fragments having a size in the range of 40 to 1200 bases are selected. A base number is assigned to each of the selected peaks in the reference data trace, and a numerical "peak file" is created with information about the peak number and migration time (or distance). This peak file is analyzed to determine a set of polynomial coefficients which will allow substantial linearization of a plot of peak number versus separation between adjacent peaks and ...

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25-07-2013 дата публикации

Signal and Detection System for Keying Applications

Номер: US20130188187A1
Принадлежит:

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product. 1. A system for differentiating the spectral response of one or more optical coatings on a substrate between a transmitter and receiver comprising:a transmitter operatively located adjacent the optical coating for transmitting a first light signal against the optical coating;a receiver operatively located adjacent the optical coating for receiving reflected light off the optical coating;receiver electronics operatively connected to the receiver for interpreting reflected light at the receiver against an authorized signal and determining if the optical coating is an authorized or unauthorized optical coating.2. The system as in wherein the transmitter is an LED light source.3. The system as in wherein the system includes at least two transmitters and each transmitter transmits a different wavelength of light against the optical coating.4. The system as in wherein each transmitter transmits light against a common optical coating and the optical coating has different reflection properties to each wavelength of light.5. The system as in wherein the authorized signal is a combination of received signals from each transmitter.6. The system as in wherein the optical coating includes at least two optical coatings and each optical coating is paired with a corresponding transmitter and receiver pair.7. The system as in wherein each transmitter of each transmitter and receiver pair emits different wavelength light.8. The system as in wherein the at least two optical coatings have different reflective properties.9. The system as in wherein each optical coating has different reflection properties.10. The system as in wherein the optical coating includes ...

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03-05-2018 дата публикации

Disposable bioassay cartridge and method of performing multiple assay steps and fluid transfer within the cartridge

Номер: US20180117582A1
Принадлежит: Alexa Inc, Angle Europe Ltd

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins.

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18-09-2014 дата публикации

Diffraction based biosensor containing two diffractive gratings

Номер: US20140271363A1
Принадлежит: Axela Inc

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

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05-08-2021 дата публикации

DISPOSABLE BIOASSAY CARTRIDGE AND METHOD OF PERFORMING MULTIPLE ASSAY STEPS AND FLUID TRANSFER WITHIN THE CARTRIDGE

Номер: US20210237050A1
Принадлежит:

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any internal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins. 135-. (canceled)36. A method for a performing biological assay , comprising:providing a disposable sample handling cartridge having at least one set of processing chambers with each set of processing chambers including an upper processing chamber located on top of a lower processing chamber separated by a porous substrate, said porous substrate projecting down into said lower processing chamber to form at least one head space in the lower processing chamber adjacent to the side of the portion of the porous substrate projecting into the lower processing chamber, the porous substrate being constructed of material containing pores selected to provide a uniform resistance to flow across its entire surface such that at a defined pressure differential across the porous substrate, liquids will pass through the pores but gases will not, the porous substrate having analyte specific receptors bound in said pores, a pneumatic port mounted on a top of the upper processing chamber, a pneumatic port mounted on a top of the lower processing chamber;applying a differential pressure between one or more reagent chambers, a sample chamber and the upper processing chamber for moving liquids containing reagents from one or more reagent chambers and liquid containing sample from the sample chamber through capillary channels to the upper processing chamber, the differential pressure being applied via pneumatic ports on top of said one or more reagent chambers and on top of said sample chamber and said pneumatic port on said upper processing chamber,applying a ...

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13-10-2016 дата публикации

Method for performing a biological assay

Номер: CA3162752A1
Принадлежит: Angle Europe Ltd, ENGLERT DAVID

The present disclosure provides a cartridge and method to move fluids within the cartridge that simplifies the design and removes the need for any intemal valves or metering devices. The design is amenable to injection molded manufacturing lowering cost for large volume manufacturing. The design can be adapted to carry out both sample preparation and detection of biological substances including nucleic acids and proteins.

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07-07-1999 дата публикации

Apparatus and method for performing sequencing of nucleic acid polymers

Номер: EP0927268A1
Принадлежит: Visible Genetics Inc

An apparatus for processing samples containing DNA to produce a sequencing fragment mixture comprises a sample processing element comprising: a thermocycling region having one or more chambers for receiving a DNA sequencing reaction mixture and forming sequencing fragments therefrom; a separation region comprising a separation matrix for separating the sequencing fragments formed in the thermocycling regions; a detection region for detection of the separated sequencing fragments; and means for regulating the temperature in the thermocycling region of the sample processing element to provide a plurality of thermal cycles, each cycle including at least a denaturation phase and an extension phase. The apparatus for processing sample can be placed in a holder which is associated with means for applying an electric field to the separation region of a sample processing apparatus placed within the holder to cause polynucleotide sequencing fragments to migrate through the separation region from the thermocycling region to the detection region; and means for detecting polynucleotide fragments within the detection region of the sample processing apparatus placed within the holder.

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11-01-2000 дата публикации

High dynamic range apparatus for separation and detection of polynucleotide fragments

Номер: US6014213A
Принадлежит: Visible Genetics Inc

A high dynamic range apparatus for separation and detection of polynucleotide fragments has a housing adapted to receive an electrophoresis gel holder containing an electrophoresis gel loaded with fluorophore-labeled samples; one or more laser diodes for providing radiation of a frequency suitable for excitation of the fluorophore which irradiates a an array of excitation/detection sites on the electrophoresis gel; an array of detectors aligned with the excitation/detection sites for collecting fluorescent emissions; and one or more components for increasing the dynamic range of the instrument by at least an order of magnitude. These components, which can be used individually or in combination include detectors that are connected to a signal processing system that modulates the period of signal integration employed so that large signals are totaled at short time intervals and smaller signals are totaled at longer time intervals; the use of a beam splitter to produces a high intensity beam of emitted light and a low intensity beam of emitted light from each excitation/detection site; and a device for modulating the intensity of the excitation beam can be used to improve the dynamic range of the instrument.

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15-09-2010 дата публикации

Methods for detecting peaks in a nucleic acid data trace

Номер: EP1981993A4
Принадлежит: Siemens Healthcare Diagnostics Inc

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22-05-2007 дата публикации

Electrophoretic trace simulator

Номер: US7222059B2

A simulated electrophoretic trace is prepared by first obtaining an input file containing an input base sequence comprising a string of letters (A, C, G and/or T) in an order corresponding to the input base sequence, and then modifying the input file using one or more functions to take into account perturbations associated with (1) changes in peak intensity as a function of base number; (2) peak shape as a function of base number; (3) peak skew; (4) spacing between peaks; (5) background; (6) noise; (7) spectral cross-talk; (8) instrumental effects and/or (9) gel electrophoresis effects to produce a modified file representing a simulated electrophoretic trace. The method may be performed using a specially adapted apparatus.

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29-08-2000 дата публикации

Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same

Номер: US6110339A
Принадлежит: Visible Genetics Inc

Separation matrices useful in the formation of solid-state mm- to cm-scale devices for the rapid, high-resolution separation of single-stranded DNA ladder bands generated by the Sanger dideoxy- or Maxam/Gilbert chemical DNA sequencing procedures are formed from a solid support (1) having a plurality of posts (4) disposed on a first major surface thereof to form an obstacle course of posts (4) and pores (5). The posts are arranged in a regular X, Y array and are separated one from another by a distance of 100 nm or less, preferably 10 to 30 nm, and are optionally separated into lanes 2. The separation matrix can be manufactured by first forming a mold, preferably a reusable mold using lithography techniques. The mold is the reverse of the desired pattern of posts and pores of the obstacle course, and is used for casting the obstacle course. The cast obstacle course is then fused to a solid support and separated from the mold. Alternatively, the separation matrix can be formed from a polymer which undergoes specific and quantifiable swelling in the presence of a selected chemical compound. In this case, the matrix is cast on a mold in a conventional manner with a spacing between posts greater than the desired final spacing of 100 nm or less. For use, a buffer solution saturated with the specific chemical agent that controls swelling is added, causing the posts to swell to a defined amount to achieve the desired separation.

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05-03-1998 дата публикации

Apparatus and method for performing sequencing of nucleic acid polymers

Номер: CA2264961A1
Принадлежит: Individual

An apparatus for processing samples containing DNA to produce a sequencing fragment mixture comprises a sample processing element comprising: a thermocycling region having one or more chambers for receiving a DNA sequencing reaction mixture and forming sequencing fragments therefrom; a separation region comprising a separation matrix for separating the sequencing fragments formed in the thermocycling regions; a detection region for detection of the separated sequencing fragments; and means for regulating the temperature in the thermocycling region of the sample processing element to provide a plurality of thermal cycles, each cycle including at least a denaturation phase and an extension phase. The apparatus for processing sample can be placed in a holder which is associated with means for applying an electric field to the separation region of a sample processing apparatus placed within the holder to cause polynucleotide sequencing fragments to migrate through the separation region from the thermocycling region to the detection region; and means for detecting polynucleotide fragments within the detection region of the sample processing apparatus placed within the holder.

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27-12-1996 дата публикации

Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same

Номер: CA2226405A1
Принадлежит: Individual

Separation matrices useful in the formation of solid-state mm- to cm-scale devices for the rapid, high-resolution separation of single-stranded DNA ladder bands generated by the Sanger dideoxy- or Maxam/Gilbert chemical DNA sequencing procedures are formed from a solid support (1) having a plurality of posts (4) disposed on a first major surface thereof to form an obstacle course of posts (4) and pores (5). The posts are arranged in a regular X, Y array and are separated one from another by a distance of 100 nm or less, preferably 10 to 30 nm, and are optionally separated into lanes 2. The separation matrix can be manufactured by first forming a mold, preferably a reusable mold using lithography techniques. The mold is the reverse of the desired pattern of posts and pores of the obstacle course, and is used for casting the obstacle course. The cast obstacle course is then fused to a solid support and separated from the mold. Alternatively, the separation matrix can be formed from a polymer which undergoes specific and quantifiable swelling in the presence of a selected chemical compound. In this case, the matrix is cast on a mold in a conventional manner with a spacing between posts greater than the desired final spacing of 100 nm or less. For use, a buffer solution saturated with the specific chemical agent that controls swelling is added, causing the posts to swell to a defined amount to achieve the desired separation.

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05-02-2003 дата публикации

Method and apparatus for dna sequencing

Номер: EP1280935A1
Автор: Alexandre M. Izmailov
Принадлежит: Visible Genetics Inc

The sequence of a target DNA molecule is determined by preparing four chain termination reaction mixtures, one for each base type. The first set of fragments, indicative of the positions of a first type of base, are labeled with a first fluorescent label. The second set of fragments, indicative of the positions of a second type of base, are labeled with a second fluorescent label different from the first fluorescent label. The third set of fragments, indicative of the positions of a third type of base, are labeled with a third fluorescent label, different from the first and second fluorescent labels or with at least two labels, including at least one fluorescent label selected from among the first, second and third fluorescent labels. The fourth set of fragments, indicative of the positions of a fourth type of base, are labeled differently from the third set of chain-termination fragments and with at least two different species of labels including at least one fluorescent label selected from among the first, second and third fluorescent labels. Thus, a total of only three fluorescent labels are required to label the DNA sequencing fragments. The first, second, third and fourth sets of chain-termination fragments are loaded onto the same lane of an electrophoresis separation medium and separated in an electric field. The separated fragments are detected in real-time as they migrate in the electrophoresis separation medium by irradiating the separated fragments with an excitation beam and collecting light emitted by the fluorescent labels in three optical channels. The signals from three optical channels are evaluated to determine a DNA sequence for the target species. This evaluation can be done with a specifically programmed computer.

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17-08-2011 дата публикации

Method for purification of nucleic acids, particularly from fixed tissue

Номер: EP2356232A1
Принадлежит: Siemens Healthcare Diagnostics Inc

The invention relates to a method for purification of nucleic acids, to a kit for performing the method according to the invention and to a new application of magnetic particles for purification of a biological sample. The method according to the invention comprises the following steps: a) accommodating of the sample in a first sample vessel in an aqueous solution and lysing of the sample under non-chaotropic conditions; suspending of first magnetic particles in the solution and inserting of the first sample vessel in a sample vessel holder, wherein the sample vessel is inserted in the annular interior space of a ring magnet associated with the sample vessel holder; separating of the solution from the magnetic particles; and isolating of the nucleic acids from the solution.

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21-10-2010 дата публикации

Diagnostic devices and related methods

Номер: WO2010120951A1
Принадлежит: Relia Diagnostic Systems, Inc.

Devices, systems, and methods for detecting the presence of one or more analytes in a sample are described. In some variations, a test strip may be used to detect and/or analyze one or more analytes in a sample. In certain variations, a test strip configured to receive a sample for detection of an analyte therein may comprise a substrate and a coating on a portion of the substrate, the coating comprising a combination of a first analyte capture agent configured to bind to a first analyte and a second analyte capture agent configured to bind to a second analyte that is different from the first analyte.

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27-12-1996 дата публикации

Nanofabricated separation matrix for analysis of biopolymers and methods of making and using same

Номер: WO1996042012A1
Принадлежит: Visible Genetics Inc.

Separation matrices useful in the formation of solid-state mm- to cm-scale devices for the rapid, high-resolution separation of single-stranded DNA ladder bands generated by the Sanger dideoxy- or Maxam/Gilbert chemical DNA sequencing procedures are formed from a solid support (1) having a plurality of posts (4) disposed on a first major surface thereof to form an obstacle course of posts (4) and pores (5). The posts are arranged in a regular X, Y array and are separated one from another by a distance of 100 nm or less, preferably 10 to 30 nm, and are optionally separated into lanes 2. The separation matrix can be manufactured by first forming a mold, preferably a reusable mold using lithography techniques. The mold is the reverse of the desired pattern of posts and pores of the obstacle course, and is used for casting the obstacle course. The cast obstacle course is then fused to a solid support and separated from the mold. Alternatively, the separation matrix can be formed from a polymer which undergoes specific and quantifiable swelling in the presence of a selected chemical compound. In this case, the matrix is cast on a mold in a conventional manner with a spacing between posts greater than the desired final spacing of 100 nm or less. For use, a buffer solution saturated with the specific chemical agent that controls swelling is added, causing the posts to swell to a defined amount to achieve the desired separation.

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25-03-1998 дата публикации

Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules

Номер: EP0830595A1
Принадлежит: Visible Genetics Inc

A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 νm wide and 1 to 10 νm in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

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26-06-2003 дата публикации

Electrophoretic trace simulator

Номер: US20030120471A1
Принадлежит: Bayer HealthCare LLC

A simulated electrophoretic trace is prepared by first obtaining an input file containing an input base sequence comprising a string of letters (A, C, G and/or T) in an order corresponding to the input base sequence, and then modifying the input file using one or more functions to take into account perturbations associated with (1) changes in peak intensity as a function of base number; (2) peak shape as a function of base number; (3) peak skew; (4) spacing between peaks; (5) background; (6) noise; (7) spectral cross-talk; (8) instrumental effects and/or (9) gel electrophoresis effects to produce a modified file representing a simulated electrophoretic trace. The method may be performed using a specially adapted apparatus.

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15-03-2012 дата публикации

Signal and detection system for keying applications

Номер: WO2012031354A1

Systems and methods for differentiating the spectral response of various optical coatings between a transmitter and receiver are described. The system is effective in determining if an optical coating produces an authorized spectral response for determining if a product having that optical coating is authorized to be used with another product.

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27-04-1999 дата публикации

Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling

Номер: US5897842A
Принадлежит: Visible Genetics Inc

An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reaction vessels, such as capillary tubes, for the preparation and thermal cycling of reaction mixtures. In order to prevent loss of the reaction mixture from the vessels during heating, the thermal cycling apparatus of the invention provides means for sealing the proximal and distal end of each reaction vessel. The proximal ends can be sealed by coupling to a pump which permits movement of the samples within the reaction vessels. As to the distal ends, the reaction vessels can be sealed by pressing the distal end of each vessel against a sealing element with a conformable surface, or by immersing the distal end of each vessel in the reservoir of liquid, preferably of an oil, that is not miscible with the reaction mixture.

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09-07-2009 дата публикации

Product, Dispenser and Method of Dispensing Product

Номер: US20090177315A1
Принадлежит: Georgia Pacific Consumer Products LP

A method of dispensing product includes determining by a processor if product loaded into a dispenser is authorized for use in the dispenser by identifying a reference indication associated with the product; in response to determination that the product is authorized, dispense a first amount of product; and in response to determination that the sheet product is unauthorized, dispense a second amount of sheet product, wherein the second amount of sheet product is different than the first amount.

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13-10-2016 дата публикации

Microporous substrate for detection of surface bound target analyte molecules

Номер: CA3162780A1
Принадлежит: Angle Europe Ltd

The present disclosure provides a microporous substrate for detection of target analyte molecules. The microporous substrate has opposed surfaces and tapered micropores extending through the microporous substrate in which the micropore openings are wider on one surface of the substrate compared to a width of the micropore openings on the opposed surface. The micropores have bound therein analyte specific receptors complementary to the target analyte molecules being detected for. The presence of the tapered pores gives an increase in the collection efficiency of light emitted from optical probes bound to target analyte molecules, when the target analyte molecules are captured by the analyte specific receptors, which is detected by a light detector spaced from the side of the substrate facing the larger pore openings compared to a light collection efficiency of light emitted from the optical probes when the micropores are straight and not tapered.

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27-12-1996 дата публикации

Microelectrophoresis chip for moving and separating nucleic acids and other charged molecules

Номер: CA2222628A1
Принадлежит: Individual

A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 µm wide and 1 to 10 µm in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

Подробнее
03-12-2009 дата публикации

Method for signal intensity correction in waveguide sensors

Номер: US20090294677A1
Принадлежит: Siemens Healthcare Diagnostics Inc

Methods are provided for enhancing the detection of analytes with waveguides by accounting for cumulative light absorptions attributable to the presence of one or more analytes in a sample as well as the waveguide material.

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25-10-2001 дата публикации

Method and apparatus for dna sequencing

Номер: CA2406618A1
Автор: Alexandre M. Izmailov
Принадлежит: Individual

The sequence of a target DNA molecule is determined by preparing four chain termination reaction mixtures, one for each base type. The first set of fragments, indicative of the positions of a first type of base, are labeled with a first fluorescent label. The second set of fragments, indicative of t he positions of a second type of base, are labeled with a second fluorescent label different from the first fluorescent label. The third set of fragments , indicative of the positions of a third type of base, are labeled with a thir d fluorescent label, different from the first and second fluorescent labels or with at least two labels, including at least one fluorescent label selected from among the first, second and third fluorescent labels. The fourth set of fragments, indicative of the positions of a fourth type of base, are labeled differently from the third set of chain-termination fragments and with at least two different species of labels including at least one fluorescent lab el selected from among the first, second and third fluorescent labels. Thus, a total of only three fluorescent labels are required to label the DNA sequencing fragments. The first, second, third and fourth sets of chain- termination fragments are loaded onto the same lane of an electrophoresis separation medium and separated in an electric field. The separated fragment s are detected in real-time as they migrate in the electrophoresis separation medium by irradiating the separated fragments with an excitation beam and collecting light emitted by the fluorescent labels in three optical channels . The signals from three optical channels are evaluated to determine a DNA sequence for the target species. This evaluation can be done with a specifically programmed computer.

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10-08-2016 дата публикации

Methods for resolving convoluted peaks in a chromatogram

Номер: EP1997050B1
Принадлежит: Siemens Healthcare Diagnostics Inc

Подробнее
26-06-2008 дата публикации

Method for signal intensity correction in waveguide sensors

Номер: WO2008035231A3
Принадлежит: Alexandre M Izmailov, Stephan Schwers

Methods are provided for enhancing the detection of analytes with waveguides by accounting for cumulative light absorptions attributable to the presence of one or more analytes in a sample as well as the waveguide material.

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14-03-2023 дата публикации

Cell recovery method and device

Номер: US11604119B1
Принадлежит: Angle Europe Ltd

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.

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15-12-2023 дата публикации

Cell recovery method and device

Номер: CA3170243A1
Принадлежит: Angle Europe Ltd

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.

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21-12-2023 дата публикации

Cell recovery method and device

Номер: WO2023241796A1
Принадлежит: Angle Europe Limited

The present disclosure provides a cell harvesting method and device for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology. The device has two configurations, one being for use in a centrifuge to centrifuge the sample, a second for controlled release of the liquid post centrifugation. The device includes a base to hold a solid support that receives cells on a top surface thereof that releasably holds a fluid chamber which has a first opening with a gasket surrounding the opening with that bears against a top surface of the support. An opening receives the liquid sample and the gasket defines an area into which the targeted cells deposit. A first cap closes the second opening during centrifugation. Post centrifugation the first cap is removed and a fluid absorbing element and cap, designed to provide controlled removal of the liquid and to prevent positioning of a tip of the absorbing element to a distance from the solid support shorter than a predefined distance is inserted into the fluid chamber.

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22-05-2024 дата публикации

Cell recovery method and device

Номер: EP4311402B1
Принадлежит: Angle Europe Ltd

Подробнее
22-07-2015 дата публикации

Methods for resolving convoluted peaks in a chromatogram

Номер: EP1997050A4
Принадлежит: Siemens Healthcare Diagnostics Inc

Подробнее
20-01-2016 дата публикации

Diffraction based biosensor containing two diffractive gratings

Номер: EP2972242A1
Принадлежит: Axela Inc

The present disclosure provides a diffraction based biosensor containing at least two diffraction gratings. The first grating is referred to as an in-coupling diffraction grating and the coherent light source (laser) is directed to illuminate the in-coupling grating, and the biosensor is configured such that a selected order of the light beam diffracted from the in-coupling diffraction grating illuminates a second biosensor grating coated with analyte-specific receptors which are selected to preferentially bind with analytes being tested for that may or may not be located in a sample being tested.

Подробнее
14-12-2023 дата публикации

Cell recovery method and device

Номер: US20230400392A1
Принадлежит: Angle Europe Ltd

The present disclosure provides a method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support. The method comprises placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support. The method further comprises subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support. The method further comprises, post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber. The method further comprises controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.

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14-05-2024 дата публикации

Cell recovery method and device

Номер: US11982601B2
Принадлежит: Angle Europe Ltd

The present disclosure provides a method of cell sedimentation and retention of target cells, for example circulating tumour cells, CTC, from a fluid sample onto a solid support. The method comprises placing a fluid medium comprising the target cells in a fluid chamber, the fluid chamber having an open end sealed against a surface of the solid support. The method further comprises subjecting the fluid medium to centrifugation within the fluid chamber to induce sedimentation of the target cells and promote cell adhesion to the surface of the solid support. The method further comprises, post-centrifugation, positioning a fluid absorbing element in the fluid chamber to remove fluid from the fluid chamber. The method further comprises controlling a flow rate of the fluid being absorbed by the fluid absorbing element such that the sedimented cells are not detached from the surface of the solid support.

Подробнее
22-01-2009 дата публикации

Methods for resolving convoluted peaks in a chromatogram

Номер: WO2007092855A9

The present invention relates to methods for resolving convoluted peaks in a chromatogram into one or more constituent peaks using peak resolution values. The methods of the invention determine empirical peak resolution values of 'well- defined' or 'isolated' peaks in the data, then extrapolate these empirical resolution values to peaks in neighboring regions to predict the number of constituent peaks at a given peak position. Predicted peak resolution values are compared to observed peak resolution values of low-resolution or convoluted peaks to determine the number of constituent peaks in the convoluted peaks. These methods enable extension of the region of data that can used for identifying nucleotide sequences, and increase base-calling accuracy in the low-resolution region (end region) of data.

Подробнее
03-12-2008 дата публикации

Methods for resolving convoluted peaks in a chromatogram

Номер: EP1997050A2
Принадлежит: Siemens Healthcare Diagnostics Inc

The present invention relates to methods for resolving convoluted peaks in a chromatogram into one or more constituent peaks using peak resolution values. The methods of the invention determine empirical peak resolution values of 'well- defined' or 'isolated' peaks in the data, then extrapolate these empirical resolution values to peaks in neighboring regions to predict the number of constituent peaks at a given peak position. Predicted peak resolution values are compared to observed peak resolution values of low-resolution or convoluted peaks to determine the number of constituent peaks in the convoluted peaks. These methods enable extension of the region of data that can used for identifying nucleotide sequences, and increase base-calling accuracy in the low-resolution region (end region) of data.

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30-11-2016 дата публикации

Diffraction based biosensor containing two diffractive gratings

Номер: EP2972242A4
Принадлежит: Axela Inc

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14-03-2023 дата публикации

Cell recovery method and device

Номер: US11604120B1
Принадлежит: Angle Europe Ltd

The present disclosure provides a cell harvesting method for the efficient sedimentation and retention of cells from liquid samples onto a solid support with low cell losses and low impact on cell morphology.

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17-07-2008 дата публикации

Method for increasing signal intensity in an optical waveguide sensor

Номер: WO2007082160A3
Автор: Alexandre M Izmailov

A sensor platform for use in sample analysis comprises a substrate (30) of refractive index m and a thin, optically transparent layer (32) of refractive index (n2) on the substrate, where n2 is greater than n1. The platform includes one of more sensing areas each for one or more capture elements. The platform also includes an immersion fluid with a refractive index n3 greater than an aqueous buffer but less than n2 to enhance the fluorescent signal of an affinity reaction. Also disclosed are an apparatus incorporating the platform and a method of using the platform.

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22-05-2024 дата публикации

Cell recovery method and device

Номер: EP4311402C0
Принадлежит: Angle Europe Ltd

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