23-06-2023 дата публикации
Номер: CN116287475A
Принадлежит:
The invention provides a nucleotide sequence combination and a kit for RT-LAMP-CRISPR isothermal detection of a brassica yellow virus and application of the nucleotide sequence combination and the kit. The nucleotide sequence combination comprises a primer group for LAMP detection, crRNA and an ssDNA-FQ probe; the RT-LAMP-CRISPR isothermal detection method for the brassica yellow virus comprises the following steps: extracting RNA (Ribonucleic Acid) of a sample to be detected, carrying out RT-LAMP isothermal amplification reaction by using the primer group, carrying out CRISPR isothermal detection on an amplification product by using crRNA and an ssDNA-FQ probe, and finally obtaining a detection result by reading fluorescence. The rapid detection kit based on the RT-LAMP-CRISPR system and the corresponding detection method are established according to the genotype characteristics of the brassica yellows virus, the method is high in sensitivity, good in specificity, low in detection limit ...
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